Name: Neisseria wadsworthii 9715
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 10498

Type strain:

Species: Neisseria wadsworthii

Strain Designation: 9715, WC 05-9715

Culture col. no.: CCUG 59855, DSM 22247, CIP 109934

Strain history: CIP <- 2009, W.J. Wolfgang, Wadsworth Cent., Albany, New-York, USA <- Albany Med. Cent., New-York, USA: strain WC 05-9715

NCBI tax ID(s): 607711 (species)

SI-ID 371500

CIP 109934, DSM 22247, CCUG 59855

Special Collections

Other strains from Neisseria wadsworthii

Section

Neisseria wadsworthii 9715 is a microaerophile, Gram-negative, coccus-shaped human pathogen that was isolated from patient's hand wound.

coccus-shaped
Gram-negative
human pathogen
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Neisseriales*
	Family	*Neisseriaceae*
	Genus	*Neisseria*
	Species	**Neisseria wadsworthii**
	Full Scientific Name (LPSN)	Neisseria wadsworthii Wolfgang et al. 2011

Information on morphological and physiological properties Morphology

[Ref.: #29848]	Gram stain	negative
[Ref.: #123902]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %98.5

[Ref.: #29848]	Cell length	1.55 µm

[Ref.: #29848]	Cell width	1.55 µm

[Ref.: #29848]	Cell shape	coccus-shaped
[Ref.: #123902]	Cell shape	coccus-shaped

[Ref.: #29848]	Motility	no
[Ref.: #123902]	Motility	no

[Ref.: #16229]

Incubation period

1-2 days

[Ref.: #29848]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16229]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #16229]

Culture medium growth

[Ref.: #16229]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16229]

Culture medium composition

expand / minimize

[Ref.: #16229]

Culture medium

BHI MEDIUM (DSMZ Medium 215)

[Ref.: #16229]

Culture medium growth

[Ref.: #16229]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16229]

Culture medium composition

expand / minimize

[Ref.: #37878]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37878]

Culture medium growth

[Ref.: #37878]

Culture medium composition

expand / minimize

[Ref.: #123902]

Culture medium

CIP Medium 72

[Ref.: #123902]

Culture medium growth

[Ref.: #123902]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #16229]	growth	37 °C
[Ref.: #29848]	growth	10-42 °C
[Ref.: #37878]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16229]	Oxygen tolerance	microaerophile
[Ref.: #29848]	Oxygen tolerance	facultative anaerobe
[Ref.: #123902]	Oxygen tolerance	obligate aerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %91.414

[Ref.: #29848]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68377]	D-fructose ChEBI	-	builds acid from	* from API NH
[Ref.: #68377]	maltose ChEBI	-	builds acid from	* from API NH
[Ref.: #29848]	nitrate ChEBI	+	reduction
[Ref.: #123902]	nitrate ChEBI	+	reduction
[Ref.: #123902]	nitrite ChEBI	-	reduction
[Ref.: #68377]	ornithine ChEBI	-	degradation	* from API NH
[Ref.: #68377]	sucrose ChEBI	-	builds acid from	* from API NH
[Ref.: #68377]	tryptophan ChEBI	-	energy source	* from API NH
[Ref.: #68377]	urea ChEBI	-	hydrolysis	* from API NH

	Metabolite production	Metabolite	Production
[Ref.: #123902]		indole ChEBI	no
[Ref.: #68377]		indole ChEBI	no	* from API NH

	Physiological tests	Metabolite	Indole test
[Ref.: #68377]		indole ChEBI	-	* from API NH

	Enzyme	Enzyme activity	EC number
[Ref.: #29848]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68377]	alkaline phosphatase	-	3.1.3.1	* from API NH, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #29848]	catalase	+	1.11.1.6
[Ref.: #123902]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #29848]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68377]	gamma-glutamyltransferase	-	2.3.2.2	* from API NH
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68377]	lipase	-		* from API NH
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68377]	ornithine decarboxylase	-	4.1.1.17	* from API NH
[Ref.: #123902]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68377]	tryptophan deaminase	-	4.1.99.1	* from API NH
[Ref.: #123902]	urease	-	3.5.1.5
[Ref.: #68377]	urease	-	3.5.1.5	* from API NH
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #16229]	6157	+	-	-	-	-	-	-	-	-	+	-	-	-
[Ref.: #16229]	5715	-	+	-	-	-	-	-	-	-	-	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123902]	12230	-	-	+	-	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16229]	Sample type/isolated from	patient's hand wound
[Ref.: #16229]	Geographic location (country and/or sea, region)	New York State, Rensselaer County
[Ref.: #16229]	Country	USA
[Ref.: #16229]	Country ISO 3 Code	USA
[Ref.: #16229]	Continent	North America

[Ref.: #62132]	Sample type/isolated from	Human wound,39-yr-old male,hand
[Ref.: #62132]	Sampling date	2005-12-16
[Ref.: #62132]	Geographic location (country and/or sea, region)	New York,Rensselaer County
[Ref.: #62132]	Country	USA
[Ref.: #62132]	Country ISO 3 Code	USA
[Ref.: #62132]	Continent	North America

[Ref.: #123902]	Sample type/isolated from	Human, Hand wound
[Ref.: #123902]	Geographic location (country and/or sea, region)	New York
[Ref.: #123902]	Country	United States of America
[Ref.: #123902]	Country ISO 3 Code	USA
[Ref.: #123902]	Continent	North America
[Ref.: #123902]	Isolation date	2005
* marker position based on {}

Isolation sources categories

#Infection	#Patient	-
#Host Body-Site	#Other	#Wound
#Host Body-Site	#Limb	#Hand

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FJ654662 (>99% sequence identity) for Neisseria from Microbeatlas

Aquatic Samples	288
Soil Samples	8
Animal Samples	1573
Plant Samples	3
Total Samples	1872

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16229]	Pathogenicity (human)	yes, in single cases
[Ref.: #16229]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123902]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16229]	Neisseria wadsworthii strain WC 05-9715 16S ribosomal RNA gene, partial sequence	FJ654662	1463	GenBank ENA	1030841 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Neisseria wadsworthii 9715	GCA_000227765	scaffold	GenBank ENA	1030841 tax ID	*
[Ref.: #66792]	Neisseria wadsworthii 9715	1030841.3	wgs	PATRIC	1030841 tax ID	*
[Ref.: #66792]	Neisseria wadsworthii 9715	2513237392	draft	JGI IMG/M	1030841 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Neisseria wadsworthii 9715 9715 NCBI: GCA_000227765.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	98.5	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	94.43	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	65.123	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	91.414	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.461	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	85.821	no

Availability in culture collections External links

[Ref.: #16229]

Culture collection no.

CCUG 59855, DSM 22247, CIP 109934

[Ref.: #79775]

SI-ID 371500

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Neisseria chenwenguii sp. nov. isolated from the rectal contents of a plateau pika (Ochotona curzoniae).	Zhang G, Yang J, Lai XH, Lu S, Jin D, Pu J, Bai X, Luo X, Xiong Y, Huang Y, Chen C, Xu J	Antonie Van Leeuwenhoek	10.1007/s10482-019-01234-2	2019	*
Phylogeny	Neisseria wadsworthii sp. nov. and Neisseria shayeganii sp. nov., isolated from clinical specimens.	Wolfgang WJ, Carpenter AN, Cole JA, Gronow S, Habura A, Jose S, Nazarian EJ, Kohlerschmidt DJ, Limberger R, Schoonmaker-Bopp D, Sproer C, Musser KA	Int J Syst Evol Microbiol	10.1099/ijs.0.022426-0	2010	*

References

#16229

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22247

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29848

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26222 (see below)

#37878 ; Curators of the CIP;
#62132 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 59855

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68377

Automatically annotated from API NH .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#79775

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID371500.1 )

#123902 Collection of Institut Pasteur ; Curators of the CIP; CIP 109934

#26222

IJSEM 91 2011 ( DOI 10.1099/ijs.0.022426-0 , PubMed 20173010 )

* These data were automatically processed and therefore are not curated

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Neisseria wadsworthii 9715 9715

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