Strain identifier

BacDive ID: 10498

Type strain: Yes

Species: Neisseria wadsworthii

Strain Designation: 9715, WC 05-9715

Strain history: CIP <- 2009, W.J. Wolfgang, Wadsworth Cent., Albany, New-York, USA <- Albany Med. Cent., New-York, USA: strain WC 05-9715

NCBI tax ID(s): 607711 (species)

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General

@ref: 16229

BacDive-ID: 10498

DSM-Number: 22247

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, Gram-negative, coccus-shaped, human pathogen

description: Neisseria wadsworthii 9715 is a microaerophile, Gram-negative, coccus-shaped human pathogen that was isolated from patient's hand wound.

NCBI tax id

  • NCBI tax id: 607711
  • Matching level: species

strain history

@refhistory
16229<- W. J. Wolfgang and V. Derbyshire, Wadsworth Center, NYS Dept. of Health, Albany, USA; WC 05-9715 <- Albany Medical Center
123902CIP <- 2009, W.J. Wolfgang, Wadsworth Cent., Albany, New-York, USA <- Albany Med. Cent., New-York, USA: strain WC 05-9715

doi: 10.13145/bacdive10498.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Neisseriales
  • family: Neisseriaceae
  • genus: Neisseria
  • species: Neisseria wadsworthii
  • full scientific name: Neisseria wadsworthii Wolfgang et al. 2011

@ref: 16229

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Neisseriales

family: Neisseriaceae

genus: Neisseria

species: Neisseria wadsworthii

full scientific name: Neisseria wadsworthii Wolfgang et al. 2011

strain designation: 9715, WC 05-9715

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
29848negative1.55 µm1.55 µmcoccus-shapedno
123902negativecoccus-shapedno
69480negative98.5

colony morphology

  • @ref: 16229
  • incubation period: 1-2 days

pigmentation

  • @ref: 29848
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16229COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
16229BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
37878MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123902CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
16229positivegrowth37
29848positivegrowth10-42
37878positivegrowth37

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16229microaerophile
29848facultative anaerobe
123902obligate aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 91.414

observation

  • @ref: 29848
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2984817632nitrate+reduction
12390217632nitrate+reduction
12390216301nitrite-reduction
6837715824D-fructose-builds acid from
6837717306maltose-builds acid from
6837717992sucrose-builds acid from
6837718257ornithine-degradation
6837716199urea-hydrolysis
6837727897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
12390235581indoleno

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
29848acid phosphatase+3.1.3.2
29848catalase+1.11.1.6
29848cytochrome oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377gamma-glutamyltransferase-2.3.2.2
68377alkaline phosphatase-3.1.3.1
68377lipase-
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
123902oxidase+
123902catalase+1.11.1.6
123902urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123902--+--++---++--------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
16229+--------+---
16229-+--------+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
16229patient's hand woundNew York State, Rensselaer CountyUSAUSANorth America
62132Human wound,39-yr-old male,handNew York,Rensselaer CountyUSAUSANorth America2005-12-16
123902Human, Hand woundNew YorkUnited States of AmericaUSANorth America2005

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host Body-Site#Other#Wound
#Host Body-Site#Limb#Hand

taxonmaps

  • @ref: 69479
  • File name: preview.99_6049.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15355;96_837;97_979;98_1155;99_6049&stattab=map
  • Last taxonomy: Neisseria
  • 16S sequence: FJ654662
  • Sequence Identity:
  • Total samples: 1872
  • soil counts: 8
  • aquatic counts: 288
  • animal counts: 1573
  • plant counts: 3

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
16229yes, in single cases1Risk group (German classification)
1239021Risk group (French classification)

Sequence information

16S sequences

  • @ref: 16229
  • description: Neisseria wadsworthii strain WC 05-9715 16S ribosomal RNA gene, partial sequence
  • accession: FJ654662
  • length: 1463
  • database: nuccore
  • NCBI tax ID: 1030841

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Neisseria wadsworthii 9715GCA_000227765scaffoldncbi1030841
66792Neisseria wadsworthii 97151030841.3wgspatric1030841
66792Neisseria wadsworthii 97152513237392draftimg1030841

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingno98.5yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no94.43yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes65.123no
69480spore-formingspore-formingAbility to form endo- or exosporesno91.414no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.461no
69480flagellatedmotile2+Ability to perform flagellated movementno85.821no

External links

@ref: 16229

culture collection no.: CCUG 59855, DSM 22247, CIP 109934

straininfo link

  • @ref: 79775
  • straininfo: 371500

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny20173010Neisseria wadsworthii sp. nov. and Neisseria shayeganii sp. nov., isolated from clinical specimens.Wolfgang WJ, Carpenter AN, Cole JA, Gronow S, Habura A, Jose S, Nazarian EJ, Kohlerschmidt DJ, Limberger R, Schoonmaker-Bopp D, Sproer C, Musser KAInt J Syst Evol Microbiol10.1099/ijs.0.022426-02010Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Humans, Molecular Sequence Data, Neisseria/chemistry/*classification/genetics/*isolation & purification, Neisseriaceae Infections/*microbiology, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Sequence Analysis, DNAPathogenicity
Phylogeny30798492Neisseria chenwenguii sp. nov. isolated from the rectal contents of a plateau pika (Ochotona curzoniae).Zhang G, Yang J, Lai XH, Lu S, Jin D, Pu J, Bai X, Luo X, Xiong Y, Huang Y, Chen C, Xu JAntonie Van Leeuwenhoek10.1007/s10482-019-01234-22019Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Lagomorpha/*microbiology, Neisseria/classification/genetics/*isolation & purification/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Rectum/*microbiology, TibetMetabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16229Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22247)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22247
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29848Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2622228776041
37878Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7733
62132Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 59855)https://www.ccug.se/strain?id=59855
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
79775Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID371500.1StrainInfo: A central database for resolving microbial strain identifiers
123902Curators of the CIPCollection of Institut Pasteur (CIP 109934)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109934