Name: Chromobacterium subtsugae PRAA4-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 10451

Type strain:

Species: Chromobacterium subtsugae

Strain Designation: PRAA4-1

Culture col. no.: DSM 17043, NRRL B-30655

Strain history: <- P. A. W. Martin, Baltimore; PRAA4-1

NCBI tax ID(s): 251747 (species)

SI-ID 406588

DSM 17043

Section

Chromobacterium subtsugae PRAA4-1 is an aerobe, mesophilic, Gram-negative animal pathogen that was isolated from Hemlock leaf litter.

Gram-negative
motile
rod-shaped
aerobe
animal pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Neisseriales*
	Family	*Chromobacteriaceae*
	Genus	*Chromobacterium*
	Species	**Chromobacterium subtsugae**
	Full Scientific Name (LPSN)	Chromobacterium subtsugae Martin et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #31894]	Gram stain	negative

[Ref.: #31894]	Cell length	2.4 µm

[Ref.: #31894]	Cell width	0.7 µm

[Ref.: #31894]	Cell shape	rod-shaped

[Ref.: #31894]	Motility	yes

[Ref.: #31894]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6704]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #6704]

Culture medium growth

[Ref.: #6704]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6704]

Culture medium composition

expand / minimize

[Ref.: #6704]

Culture medium

TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)

[Ref.: #6704]

Culture medium growth

[Ref.: #6704]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6704]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #6704]	growth	28 °C
[Ref.: #31894]	growth	04-40 °C
[Ref.: #31894]	optimum	26.5 °C

[Ref.: #6704]	Temperature range	mesophilic
[Ref.: #31894]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31894]			optimum	7.25
[Ref.: #31894]			growth	4.5-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31894]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31894]		NaCl		growth	0-2	%
[Ref.: #31894]		NaCl		optimum	1	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31894]	acetate ChEBI	+	carbon source
[Ref.: #31894]	alanine ChEBI	+	carbon source
[Ref.: #31894]	asparagine ChEBI	+	carbon source
[Ref.: #31894]	aspartate ChEBI	+	carbon source
[Ref.: #31894]	decanoate ChEBI	+	carbon source
[Ref.: #31894]	fructose ChEBI	+	carbon source
[Ref.: #31894]	glucose ChEBI	+	carbon source
[Ref.: #31894]	glutamate ChEBI	+	carbon source
[Ref.: #31894]	histidine ChEBI	+	carbon source
[Ref.: #31894]	inosine ChEBI	+	carbon source
[Ref.: #31894]	lactate ChEBI	+	carbon source
[Ref.: #31894]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #31894]	nitrate ChEBI	+	reduction
[Ref.: #31894]	propionate ChEBI	+	carbon source
[Ref.: #31894]	serine ChEBI	+	carbon source
[Ref.: #31894]	threonine ChEBI	+	carbon source
[Ref.: #31894]	trehalose ChEBI	+	carbon source
[Ref.: #31894]	tween 40 ChEBI	+	carbon source
[Ref.: #31894]	tween 80 ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6704]	Sample type/isolated from	Hemlock leaf litter
[Ref.: #6704]	Geographic location (country and/or sea, region)	Maryland
[Ref.: #6704]	Country	USA
[Ref.: #6704]	Country ISO 3 Code	USA
[Ref.: #6704]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6704]	Pathogenicity (animal)	yes
[Ref.: #6704]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #31894]	Chromobacterium subtsugae strain PRAA4-1 16S ribosomal RNA gene, complete sequence	AY344056	1440	GenBank	251747 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Chromobacterium subtsugae PRAA4-1	GCA_001676875	contig	GenBank	251747 tax ID	*
[Ref.: #66792]	Chromobacterium subtsugae PRAA4-1	2843400765	draft	JGI IMG/M	251747 tax ID	*

[Ref.: #31894]

GC-content

64.5 mol%

Availability in culture collections External links

[Ref.: #6704]

Culture collection no.

DSM 17043, NRRL B-30655

[Ref.: #79728]

SI-ID 406588

Literature:

	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudogulbenkiania subflava gen. nov., sp. nov., isolated from a cold spring.	Lin MC, Chou JH, Arun AB, Young CC, Chen WM	Int J Syst Evol Microbiol	10.1099/ijs.0.65755-0	2008	*
Phylogeny	Chromobacterium haemolyticum sp. nov., a strongly haemolytic species.	Han XY, Han FS, Segal J	Int J Syst Evol Microbiol	10.1099/ijs.0.64681-0	2008	*
Phylogeny	Chromobacterium subtsugae sp. nov., a betaproteobacterium toxic to Colorado potato beetle and other insect pests.	Martin PAW, Gundersen-Rindal D, Blackburn M, Buyer J	Int J Syst Evol Microbiol	10.1099/ijs.0.64611-0	2007	*
	The genome of the insecticidal Chromobacterium subtsugae PRAA4-1 and its comparison with that of Chromobacterium violaceum ATCC 12472.	Blackburn MB, Sparks ME, Gundersen-Rindal DE	Genom Data	10.1016/j.gdata.2016.08.013	2016	*
	Identification of N-acyl-l-homoserine lactones produced by non-pigmented Chromobacterium aquaticum CC-SEYA-1(T) and pigmented Chromobacterium subtsugae PRAA4-1(T).	Rekha PD, Young CC, Arun AB	3 Biotech	10.1007/s13205-011-0029-1	2011	*

References

#6704

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17043

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31894

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28153 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#79728

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406588.1 )

#28153

IJSEM 993 2007 ( DOI 10.1099/ijs.0.64611-0 , PubMed 17473247 )

* These data were automatically processed and therefore are not curated

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