Chromobacterium subtsugae (DSM 17043, NRRL B-30655, PRAA4-1)

Name: Chromobacterium subtsugae (DSM 17043, NRRL B-30655, PRAA4-1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 10451

Type strain

Strain Designation PRAA4-1

Culture col. no. DSM 17043 NRRL B-30655 PRAA4-1

NCBI tax ID(s) 251747

Special Collections DSMZ Literature strains

History DSMZ <- P. A. W. Martin, Baltimore; PRAA4-1

Links

Chromobacterium subtsugae PRAA4-1 is an aerobe, Gram-negative, motile animal pathogen that was isolated from Hemlock leaf litter.

Gram-negative motile rod-shaped aerobe animal pathogen genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 406588

DSM 17043

@ref 20215

Last LPSN update 2026-05-29 08:27:20

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Neisseriales

Family Chromobacteriaceae

Genus Chromobacterium

Species Chromobacterium subtsugae

Full scientific name Chromobacterium subtsugae Martin et al. 2007

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
31894	negative	2.4 µm	0.7 µm	rod-shaped
125439					92.024
125439	negative				99.269

Pigmentation

31894

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6704	CASO AGAR (MERCK 105458) (DSMZ Medium 220)		Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
6704	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)		Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
6704	positive	growth	28
31894	positive	growth	04-40
31894	positive	optimum	26.5

Culture pH

@ref	Ability	Type	PH	PH range
31894	positive	optimum	7.25
31894	positive	growth	4.5-9.0	alkaliphile

Physiology and metabolism

Oxygen tolerance

31894

Oxygen toleranceaerobe

Spore formation

@ref	Spore formation	Confidence
125439		99.865

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31894	NaCl	positive	growth	0-2 %
31894	NaCl	positive	optimum	1 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31894	30089 ChEBI	acetate	+	carbon source
31894	16449 ChEBI	alanine	+	carbon source
31894	22653 ChEBI	asparagine	+	carbon source
31894	35391 ChEBI	aspartate	+	carbon source
31894	27689 ChEBI	decanoate	+	carbon source
31894	28757 ChEBI	fructose	+	carbon source
31894	17234 ChEBI	glucose	+	carbon source
31894	29987 ChEBI	glutamate	+	carbon source
31894	27570 ChEBI	histidine	+	carbon source
31894	17596 ChEBI	inosine	+	carbon source
31894	24996 ChEBI	lactate	+	carbon source
31894	506227 ChEBI	N-acetylglucosamine	+	carbon source
31894	17632 ChEBI	nitrate	+	reduction
31894	17272 ChEBI	propionate	+	carbon source
31894	17822 ChEBI	serine	+	carbon source
31894	26986 ChEBI	threonine	+	carbon source
31894	27082 ChEBI	trehalose	+	carbon source
31894	53423 ChEBI	tween 40	+	carbon source
31894	53426 ChEBI	tween 80	+	carbon source

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6704	Hemlock leaf litter	Maryland	USA	USA	North America

Interaction and safety

Risk assessment

@ref	Pathogenicity animal	Biosafety level	Biosafety level comment
6704		1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	IMG accession	NCBI tax ID	Score
66792	ASM167687v1 assembly for Chromobacterium subtsugae PRAA4-1	contig	GCA_001676875	2843400765	251747	63.01

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
31894	Chromobacterium subtsugae strain PRAA4-1 16S ribosomal RNA gene, complete sequence	AY344056	1440		251747

GC content

31894

GC-content (mol%)64.5

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM167687v1 assembly for Chromobacterium subtsugae PRAA4-1
NCBI: GCA_001676875

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	67.48	no
125439	gram_stain	BacteriaNetⓘ	negative	99.27	no
125439	motility	BacteriaNetⓘ	yes	92.02	no
125439	spore_formation	BacteriaNetⓘ	no	99.87	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Transcriptome	The mosquito adulticidal Chromobacterium sp. Panama causes transgenerational impacts on fitness parameters and elicits xenobiotic gene responses.	Short SM, van Tol S, Smith B, Dong Y, Dimopoulos G.	Parasit Vectors	10.1186/s13071-018-2822-8	2018
Pathogenicity	High-level production of violacein by the newly isolated Duganella violaceinigra str. NI28 and its impact on Staphylococcus aureus.	Choi SY, Kim S, Lyuck S, Kim SB, Mitchell RJ.	Sci Rep	10.1038/srep15598	2015
	The genome of the insecticidal Chromobacterium subtsugae PRAA4-1 and its comparison with that of Chromobacterium violaceum ATCC 12472.	Blackburn MB, Sparks ME, Gundersen-Rindal DE	Genom Data	10.1016/j.gdata.2016.08.013	2016
	Identification of N-acyl-l-homoserine lactones produced by non-pigmented Chromobacterium aquaticum CC-SEYA-1(T) and pigmented Chromobacterium subtsugae PRAA4-1(T).	Rekha PD, Young CC, Arun AB	3 Biotech	10.1007/s13205-011-0029-1	2011
Phylogeny	Pseudogulbenkiania subflava gen. nov., sp. nov., isolated from a cold spring.	Lin MC, Chou JH, Arun AB, Young CC, Chen WM	Int J Syst Evol Microbiol	10.1099/ijs.0.65755-0	2008
Phylogeny	Chromobacterium haemolyticum sp. nov., a strongly haemolytic species.	Han XY, Han FS, Segal J	Int J Syst Evol Microbiol	10.1099/ijs.0.64681-0	2008
Phylogeny	Chromobacterium subtsugae sp. nov., a betaproteobacterium toxic to Colorado potato beetle and other insect pests.	Martin PAW, Gundersen-Rindal D, Blackburn M, Buyer J	Int J Syst Evol Microbiol	10.1099/ijs.0.64611-0	2007

References

#6704	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17043
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28153	IJSEM 993 2007 ( DOI 10.1099/ijs.0.64611-0 , PubMed 17473247 )
#31894	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28153
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Chromobacterium subtsugae (DSM 17043, NRRL B-30655, PRAA4-1)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001676875

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media