Name: Aquaspirillum bipunctata D-409
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 10440

Type strain:

Species: Aquaspirillum bipunctata

Strain Designation: D-409

Culture col. no.: DSM 12821, CIP 107339

Strain history: CIP <- 2002, DSMZ <- G.A. Dubinina

Other strains from Aquaspirillum bipunctata

A. bipunctata D-411, DSM 12822

Section

Aquaspirillum bipunctata D-409 is a mesophilic, Gram-negative, motile Proteobacterium that was isolated from industrial sewage with a high content of thiosulfate and sulfide.

Gram-negative
motile
rod-shaped
mesophilic

Information on the name and the taxonomic classification. Name and taxonomic classification

[Ref.: #4836]	Domain	Bacteria
[Ref.: #4836]	Phylum	Proteobacteria
[Ref.: #4836]	Class	Betaproteobacteria
[Ref.: #4836]	Order	Neisseriales
[Ref.: #4836]	Family	Chromobacteriaceae
[Ref.: #4836]	Genus	Aquaspirillum
[Ref.: #4836]	Species	Aquaspirillum bipunctata
[Ref.: #4836]	Full Scientific Name	Aquaspirillum bipunctata Dubinia et al.
[Ref.: #4836]	Strain Designation	D-409

Information on morphological and physiological properties Morphology

[Ref.: #115946]	Gram stain	negative

[Ref.: #115946]	Cell shape	rod-shaped

[Ref.: #115946]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4836]

Culture medium

AQUASPIRILLUM MEDIUM II (DSMZ Medium 1495)

[Ref.: #4836]

Culture medium growth

[Ref.: #4836]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4836]

Culture medium composition

expand / minimize

[Ref.: #4836]

Culture medium

SPIRILLUM MEDIUM (DSMZ Medium 37)

[Ref.: #4836]

Culture medium growth

[Ref.: #4836]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4836]

Culture medium composition

expand / minimize

[Ref.: #36603]

Culture medium

MEDIUM 65 - for Prolinoborus fasciculus

[Ref.: #36603]

Culture medium growth

[Ref.: #36603]

Culture medium composition

expand / minimize

[Ref.: #115946]

Culture medium

CIP Medium 65

[Ref.: #115946]

Culture medium growth

[Ref.: #115946]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #4836]		growth	28 °C
[Ref.: #36603]		growth	30 °C
[Ref.: #115946]		growth	15-41 °C
[Ref.: #115946]	no	growth	5 °C

[Ref.: #4836]	Temperature range	mesophilic
[Ref.: #36603]	Temperature range	mesophilic
[Ref.: #115946]	Temperature range	psychrophilic

Information on physiology and metabolism Physiology and metabolism

	Salt	Tested relation		Salt conc.
[Ref.: #115946]	NaCl		growth	0	%
[Ref.: #115946]	NaCl	no	growth	2	%
[Ref.: #115946]	NaCl	no	growth	4	%
[Ref.: #115946]	NaCl	no	growth	6	%
[Ref.: #115946]	NaCl	no	growth	8	%
[Ref.: #115946]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #115946]	esculin ChEBI	-	hydrolysis
[Ref.: #115946]	glucose ChEBI	+	fermentation
[Ref.: #115946]	lactose ChEBI	-	fermentation
[Ref.: #115946]	nitrate ChEBI	-	reduction
[Ref.: #115946]	nitrate ChEBI	-	respiration
[Ref.: #115946]	nitrite ChEBI	-	reduction
[Ref.: #115946]	sodium thiosulfate ChEBI	-	builds gas from

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #115946]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #115946]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #115946]		acetoin ChEBI	-
[Ref.: #115946]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #115946]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #115946]	amylase	-
[Ref.: #115946]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #115946]	caseinase	-	3.4.21.50
[Ref.: #115946]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #115946]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #115946]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #115946]	gelatinase	-
[Ref.: #115946]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #115946]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #115946]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #115946]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #115946]	oxidase	+
[Ref.: #115946]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #115946]	tryptophan deaminase	-
[Ref.: #115946]	tween esterase	-
[Ref.: #115946]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #115946]	10277	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4836]	Sample type/isolated from	industrial sewage with a high content of thiosulfate and sulfide
[Ref.: #4836]	Geographic location (country and/or sea, region)	Voronezk state region
[Ref.: #4836]	Country	Russia
[Ref.: #4836]	Country ISO 3 Code	RUS
[Ref.: #4836]	Continent	Europe

[Ref.: #115946]	Sample type/isolated from	Environment, Industrial sewage with a high content of thiosulfate and sulfide
[Ref.: #115946]	Geographic location (country and/or sea, region)	Voronezk state region
[Ref.: #115946]	Country	Russian Federation
[Ref.: #115946]	Country ISO 3 Code	RUS
[Ref.: #115946]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	#Industrial wastewater
#Condition	#Sulfuric	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #115946]

Biosafety level

Risk group (French classification)

Availability in culture collections External links

[Ref.: #4836]

Culture collection no.

DSM 12821, CIP 107339

References

#4836

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12821

#36603 ; Curators of the CIP;

#68382

Automatically annotated from API zym .

#115946 Collection of Institut Pasteur ; Curators of the CIP; CIP 107339
* These data were automatically processed and therefore are not curated

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