Strain identifier

BacDive ID: 10440

Type strain: No

Species: Aquaspirillum bipunctata

Strain Designation: D-409

Strain history: CIP <- 2002, DSMZ <- G.A. Dubinina

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General

@ref: 4836

BacDive-ID: 10440

DSM-Number: 12821

keywords: Gram-negative, motile, rod-shaped

description: Aquaspirillum bipunctata D-409 is a Gram-negative, motile, rod-shaped Proteobacterium that was isolated from industrial sewage with a high content of thiosulfate and sulfide.

strain history

@refhistory
4836<- G. A. Dubinina
115946CIP <- 2002, DSMZ <- G.A. Dubinina

doi: 10.13145/bacdive10440.20240510.9

Name and taxonomic classification

@ref: 4836

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Neisseriales

family: Chromobacteriaceae

genus: Aquaspirillum

species: Aquaspirillum bipunctata

full scientific name: Aquaspirillum bipunctata Dubinia et al.

strain designation: D-409

type strain: no

Morphology

cell morphology

  • @ref: 115946
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4836AQUASPIRILLUM MEDIUM II (DSMZ Medium 1495)yeshttps://mediadive.dsmz.de/medium/1495Name: AQUASPIRILLUM MEDIUM II (DSMZ Medium 1495) Composition: Agar 15.0 g/l Peptone 2.0 g/l Succinic acid 1.0 g/l (NH4)2SO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l CaCl2 x 2 H2O 0.03 g/l EDTA 0.005 g/l FeSO4 x 6 H2O 0.0022 g/l Biotin 0.0002 g/l CoCl2 x 6 H2O 0.0002 g/l Riboflavin 0.0001 g/l ZnSO4 x 7 H2O 0.0001 g/l Pyridoxine hydrochloride 0.0001 g/l Nicotinic acid 5e-05 g/l Thiamine HCl 5e-05 g/l Para-aminobenzoic acid 5e-05 g/l DL-Pantothenic acid 5e-05 g/l MnCl2 x 4 H2O 3e-05 g/l H3BO3 3e-05 g/l CuCl2 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l Folic acid 2e-05 g/l Vitamin B12 1e-06 g/l Distilled water
4836SPIRILLUM MEDIUM (DSMZ Medium 37)yeshttps://mediadive.dsmz.de/medium/37Name: SPIRILLUM MEDIUM (DSMZ Medium 37) Composition: Peptone 10.0 g/l (NH4)2SO4 1.0 g/l Succinic acid 1.0 g/l MgSO4 x 7 H2O 1.0 g/l FeCl3 x 6 H2O 0.002 g/l MnSO4 x H2O 0.002 g/l Distilled water
36603MEDIUM 65 - for Prolinoborus fasciculusyesDistilled water make up to (1000.000 ml);Manganese II sulphate monohydrate(2.000 mg);Magnesium sulphate heptahydrate (1.000 g);Agar (15.000 g);Ammonium sulphate (1.000 g);Peptone (10.000 g);Succinic acid (1.680 g);Ferric III chloride hexahydrate (2.000mg)
115946CIP Medium 65yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=65

culture temp

@refgrowthtypetemperature
4836positivegrowth28
36603positivegrowth30
115946positivegrowth15-41
115946nogrowth5

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
115946NaClpositivegrowth0 %
115946NaClnogrowth2 %
115946NaClnogrowth4 %
115946NaClnogrowth6 %
115946NaClnogrowth8 %
115946NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1159464853esculin-hydrolysis
11594617234glucose+fermentation
11594617716lactose-fermentation
11594617632nitrate-reduction
11594616301nitrite-reduction
115946132112sodium thiosulfate-builds gas from
11594617632nitrate-respiration

antibiotic resistance

  • @ref: 115946
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 115946
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11594615688acetoin-
11594617234glucose-

enzymes

@refvalueactivityec
115946oxidase+
115946beta-galactosidase-3.2.1.23
115946alcohol dehydrogenase-1.1.1.1
115946gelatinase-
115946amylase-
115946DNase-
115946caseinase-3.4.21.50
115946catalase+1.11.1.6
115946tween esterase-
115946gamma-glutamyltransferase+2.3.2.2
115946lecithinase-
115946lipase-
115946lysine decarboxylase-4.1.1.18
115946ornithine decarboxylase-4.1.1.17
115946phenylalanine ammonia-lyase-4.3.1.24
115946tryptophan deaminase-
115946urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
115946-+++-+----++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
4836industrial sewage with a high content of thiosulfate and sulfideVoronezk state regionRussiaRUSEurope
115946Environment, Industrial sewage with a high content of thiosulfate and sulfideVoronezk state regionRussian FederationRUSEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Industrial wastewater
#Condition#Sulfuric

Safety information

risk assessment

  • @ref: 115946
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 4836

culture collection no.: DSM 12821, CIP 107339

Reference

@idauthorscataloguedoi/urltitle
4836Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12821)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12821
36603Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4764
68382Automatically annotated from API zym
115946Curators of the CIPCollection of Institut Pasteur (CIP 107339)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107339