Name: Nakamurella multipartita Y-104
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 10173

Type strain:

Species: Nakamurella multipartita

Strain Designation: Y-104

Culture col. no.: DSM 44233, ATCC 700099, CIP 104796, JCM 9543, CCM 4621, FERM P-14709, NBRC 105858

Strain history: CIP <- 1996, K. Nakamura, Nat. Inst. of Bioscience and Human Technol., Japan: strain Y-104

NCBI tax ID(s): 479431 (strain), 53461 (species)

SI-ID 46213

ATCC 700099, JCM 9543, CIP 104796, DSM 44233, CCM 4621

Section

Nakamurella multipartita Y-104 is an obligate aerobe, mesophilic bacterium that was isolated from activated sludge.

mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Nakamurellales*
	Family	*Nakamurellaceae*
	Genus	*Nakamurella*
	Species	**Nakamurella multipartita**
	Full Scientific Name (LPSN)	Nakamurella multipartita (Yoshimi et al. 1996) Tao et al. 2004

Synonym

Microsphaera multipartita

Information on morphological and physiological properties Morphology

[Ref.: #121173]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #121173]	Cell shape	coccus-shaped

[Ref.: #121173]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %91.125

[Ref.: #20027]	Colony color	Cream (9001)
[Ref.: #20027]	Incubation period	10-14 days
[Ref.: #20027]	Cultivation medium used	5006

[Ref.: #20027]	Colony color	Cream (9001)
[Ref.: #20027]	Incubation period	10-14 days
[Ref.: #20027]	Cultivation medium used	5425

[Ref.: #20027]	Colony color	Cream (9001)
[Ref.: #20027]	Incubation period	10-14 days
[Ref.: #20027]	Cultivation medium used	5428

[Ref.: #20027]	Colony color	Cream (9001)
[Ref.: #20027]	Incubation period	10-14 days
[Ref.: #20027]	Cultivation medium used	5530

[Ref.: #20027]	Colony color	Cream (9001)
[Ref.: #20027]	Incubation period	10-14 days
[Ref.: #20027]	Cultivation medium used	ISP 2

[Ref.: #20027]	Multicellular complex forming ability	no
[Ref.: #20027]	Medium Name (multicellularity)	5006

[Ref.: #20027]	Multicellular complex forming ability	no
[Ref.: #20027]	Medium Name (multicellularity)	5425

[Ref.: #20027]	Multicellular complex forming ability	no
[Ref.: #20027]	Medium Name (multicellularity)	5428

[Ref.: #20027]	Multicellular complex forming ability	no
[Ref.: #20027]	Medium Name (multicellularity)	5530

[Ref.: #20027]	Medium Name (multicellularity)	ISP 2

Multimedia content

[Ref.: #66793]

Caption:

electron microscopic image

[Ref.: #66793]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11495]

Culture medium

RICH MEDIUM (DSMZ Medium 736)

[Ref.: #11495]

Culture medium growth

[Ref.: #11495]

Culture medium link

Medium recipe at MediaDive

[Ref.: #11495]

Culture medium composition

expand / minimize

[Ref.: #11495]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #11495]

Culture medium growth

[Ref.: #11495]

Culture medium link

Medium recipe at MediaDive

[Ref.: #11495]

Culture medium composition

expand / minimize

[Ref.: #20027]

Culture medium

ISP 2

[Ref.: #20027]

Culture medium growth

[Ref.: #20027]

Culture medium composition

expand / minimize

[Ref.: #20027]

Culture medium

5006

[Ref.: #20027]

Culture medium growth

[Ref.: #20027]

Culture medium composition

expand / minimize

[Ref.: #20027]

Culture medium

5425

[Ref.: #20027]

Culture medium growth

[Ref.: #20027]

Culture medium composition

expand / minimize

[Ref.: #20027]

Culture medium

5428

[Ref.: #20027]

Culture medium growth

[Ref.: #20027]

Culture medium

5530

[Ref.: #20027]

Culture medium growth

[Ref.: #41306]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #41306]

Culture medium growth

[Ref.: #41306]

Culture medium composition

expand / minimize

[Ref.: #121173]

Culture medium

CIP Medium 135

[Ref.: #121173]

Culture medium growth

[Ref.: #121173]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121173]

Culture medium

CIP Medium 216

[Ref.: #121173]

Culture medium growth

[Ref.: #121173]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121173]

Culture medium

CIP Medium 3

[Ref.: #121173]

Culture medium growth

[Ref.: #121173]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #11495]		growth	28 °C
[Ref.: #20027]		optimum	28 °C
[Ref.: #41306]		growth	30 °C
[Ref.: #67770]		growth	25 °C
[Ref.: #121173]		growth	22-37 °C
[Ref.: #121173]	no	growth	10 °C
[Ref.: #121173]	no	growth	41 °C
[Ref.: #121173]	no	growth	45 °C

[Ref.: #11495]	Temperature range	mesophilic
[Ref.: #20027]	Temperature range	mesophilic
[Ref.: #41306]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #121173]	Temperature range	psychrophilic
[Ref.: #121173]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #121173]

Oxygen tolerance

obligate aerobe

[Ref.: #20027]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	yes Confidence in %99
[Ref.: #69480]	Ability of spore formation	no Confidence in %90.898

[Ref.: #67770]

Observation

quinones: MK-8(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]	D-sorbitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E, API Coryne
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #121173]	hippurate ChEBI	-	hydrolysis
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]	melezitose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121173]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #121173]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E, API Coryne
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #121173]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #121173]	acetoin ChEBI	-
[Ref.: #68368]	acetoin ChEBI	-		* from API 20E
[Ref.: #68368]	indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121173]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121173]	amylase	-
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #121173]	beta-galactosidase	+	3.2.1.23
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #121173]	caseinase	-	3.4.21.50
[Ref.: #121173]	catalase	+	1.11.1.6
[Ref.: #121173]	coagulase	-
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #121173]	DNase	-
[Ref.: #121173]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #121173]	gelatinase	-
[Ref.: #68379]	gelatinase	-		* from API Coryne, API 20E
[Ref.: #121173]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #121173]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121173]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #121173]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #121173]	oxidase	-
[Ref.: #121173]	protease	-
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121173]	tryptophan deaminase	-
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #121173]	tween esterase	+
[Ref.: #121173]	urease	+	3.5.1.5
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne, API 20E
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.