Name: Gilliamella bombi H14-35
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140363

Type strain:

Species: Gilliamella bombi

Strain Designation: H14-35, R-54662

Culture col. no.: DSM 104030, LMG 29879

Strain history: <- P. Vandamme, LMU Ghent, Laboratory of Microbiology, Ghent, Belgium; R-54662

NCBI tax ID(s): 1908521 (species)

SI-ID 399621

DSM 104030

Section

Gilliamella bombi H14-35 is a mesophilic bacterium that was isolated from bumblebee gut.

mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Orbales*
	Family	*Orbaceae*
	Genus	*Gilliamella*
	Species	**Gilliamella bombi**
	Full Scientific Name (LPSN)	Gilliamella bombi Praet et al. 2017

Information on culture and growth conditions Culture and growth conditions

[Ref.: #42970]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #42970]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #42970]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #42970]			growth	37 °C

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Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68373]	acetate ChEBI	-	assimilation	* from API CAM
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68373]	citrate ChEBI	-	assimilation	* from API CAM
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE, API CAM
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68373]	hippurate ChEBI	+	hydrolysis	* from API CAM
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE, API CAM
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE, API CAM
[Ref.: #68373]	propionate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	succinate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	triphenyltetrazolium chloride ChEBI	+	reduction	* from API CAM
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE, API CAM
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #68373]	cefazolin ChEBI	yes	224	µg	* from API CAM
[Ref.: #68373]	erythromycin ChEBI	yes	14	µg	* from API CAM
[Ref.: #68373]	nalidixic acid ChEBI	yes	84	µg	* from API CAM

	Metabolite production	Metabolite	Production
[Ref.: #68373]		hydrogen sulfide ChEBI	no	* from API CAM
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68373]	alkaline phosphatase	-	3.1.3.1	* from API CAM
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #68373]	catalase	-	1.11.1.6	* from API CAM
[Ref.: #68369]	cytochrome oxidase	-	1.9.3.1	* from API 20NE
[Ref.: #68373]	esterase	-		* from API CAM
[Ref.: #68373]	gamma-glutamyltransferase	+	2.3.2.2	* from API CAM
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #68373]	L-arginine arylamidase	+		* from API CAM
[Ref.: #68373]	L-aspartate arylamidase	-	3.4.11.21	* from API CAM
[Ref.: #68373]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API CAM
[Ref.: #68373]	urease	-	3.5.1.5	* from API CAM, API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #42970]	24518	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® CAM:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Reduction of nitrateNIT	EsteraseEST	Hippuric acidHIP	gamma-Glutamyl transferaseGGT	Reduction of Triphenyl tetrazolium chlorideTTC	Pyrrolidonyl arylamidasePYRA	L-arginine arylamidaseArgA	L-aspartic acid arylamidaseAspA	Alkaline phosphatasePAL	H2S productionH2S	Assimilation of D-glucoseGLU	Assimilation of succinateSUT	Growth inhibitionNAL	Growth inhibitionCFZ	Assimilation of acetateACE	Assimilation of propionic acidPROP	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Erythromycin resistance (+) sensitivity (-)ERO	CatalaseCAT
[Ref.: #42970]	3840	-	-	-	+	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42970]	Sample type/isolated from	bumblebee gut
[Ref.: #42970]	Geographic location (country and/or sea, region)	Botanical Garden Ledeganck, Ghent (51.0368° N/3.7221° E)
[Ref.: #42970]	Country	Belgium
[Ref.: #42970]	Country ISO 3 Code	BEL
[Ref.: #42970]	Continent	Europe
[Ref.: #42970]	Geographic location	51.0368°/3.7221°
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

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Global distribution of 16S sequence LT631738 (>99% sequence identity) for Gilliamella from Microbeatlas

Aquatic Samples	496
Soil Samples	164
Animal Samples	5938
Plant Samples	136
Total Samples	6734

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #42970]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42970]	Gilliamella sp. R-54662 partial 16S rRNA gene, strain LMG29879, isolate R-54662	LT631738	1434	ENA	1908521 tax ID

[Ref.: #42970]

GC-content

34.6 mol%

sequence analysis

Availability in culture collections External links

[Ref.: #42970]

Culture collection no.

DSM 104030, LMG 29879

[Ref.: #96661]

SI-ID 399621

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Gilliamella intestini sp. nov., Gilliamella bombicola sp. nov., Gilliamella bombi sp. nov. and Gilliamella mensalis sp. nov.: Four novel Gilliamella species isolated from the bumblebee gut.	Praet J, Cnockaert M, Meeus I, Smagghe G, Vandamme P	Syst Appl Microbiol	10.1016/j.syapm.2017.03.003	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42970

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104030

#68369

Automatically annotated from API 20NE .

#68373

Automatically annotated from API CAM .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96661

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399621.1 )

* These data were automatically processed and therefore are not curated

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