Mycoplasmopsis bovis (DSM 22781, ATCC 25523, NCTC 10131)

Name: Mycoplasmopsis bovis (DSM 22781, ATCC 25523, NCTC 10131)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 8597

Type strain

Strain Designation Donetta, PG 45

Culture col. no. DSM 22781 ATCC 25523 NCTC 10131 Donetta PG 45

NCBI tax ID(s) 28903

Special Collections DSMZ

History <- NCTC; NCTC 10131 <- Public Health Laboratory Services, Colindale, London, United Kingdom <- D. G. ff. Edward, Public Health Laboratory, Dulwich Hospital, London, United Kingdom <- M. E. Tourtellotte <- W. Plastridge

Links

Mycoplasmopsis bovis Donetta is an animal pathogen that was isolated from mammal, udder of a cow with mastitis.

animal pathogen 16S sequence Bacteria

Name and taxonomic classification

SI-ID 38578

DSM 22781,ATCC 25523,KCTC 2612,NCTC 10131

@ref 20215

Last LPSN update 2026-02-09 11:19:44

Domain Bacteria

Phylum Mycoplasmatota

Class Mollicutes

Order Mycoplasmoidales

Family Metamycoplasmataceae

Genus Mycoplasmopsis

Species Mycoplasmopsis bovis

Full scientific name Mycoplasmopsis bovis (Hale et al. 1962) Gupta et al. 2018

Synonyms (2)

"Mycoplasma agalactiae subsp. bovis"
Mycoplasma bovis

BacDive ID	Other strains from **Mycoplasmopsis bovis** (2)	Type strain
175203	M. bovis Madison, DSM 116317
175204	M. bovis 70-213 (Del Giudice), DSM 116318

Morphology

Colony morphology

16514

Incubation period2-3 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
16514	SP4-Z MEDIUM (DSMZ Medium 1076b)		Medium recipe at MediaDive	Name: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
16514	positive	growth	37

Physiology and metabolism

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Disease	-
#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body-Site	-	-

Isolation

16514

Sample typemammal, udder of a cow with mastitis

Taxonmaps

69479

Global distribution of 16S sequence AJ419905 (>99% sequence identity) for Mycoplasmopsis from Microbeatlas

Aquatic Samples	330
Soil Samples	237
Animal Samples	2146
Plant Samples	38
Total Samples	2751

Interaction and safety

Risk assessment

@ref	Pathogenicity animal	Biosafety level	Biosafety level comment
16514		2	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Mycoplasma bovis 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AY729934	941		28903
16514	Mycoplasma bovis 16S rRNA gene, strain PG45	AJ419905	1388		28903

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Toward a Method for Harmonized Susceptibility Testing of Mycoplasma bovis by Broth Microdilution.	Gutgemann F, Muller A, Churin Y, Kumm F, Braun AS, Yue M, Eisenberg T, Entorf M, Peters T, Kehrenberg C.	J Clin Microbiol	10.1128/jcm.01905-22	2023
Genetics	Enhanced metagenomic surveillance for bovine respiratory disease pathogens and antimicrobial resistance by hybridization capture sequencing.	Russell JN, Kos D, Yacoub E, Sies AN, Warr B, Jelinski M, Ruzzini A, Cameron ADS.	Appl Environ Microbiol	10.1128/aem.00977-25	2025
	Temperature impacts the bovine ex vivo immune response towards Mycoplasmopsis bovis.	Demoulins T, Yimthin T, Lindtke D, Eggerschwiler L, Siegenthaler R, Labroussaa F, Jores J.	Vet Res	10.1186/s13567-024-01272-3	2024
	Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes.	Nishi K, Gondaira S, Hirano Y, Ohashi M, Sato A, Matsuda K, Iwasaki T, Kanda T, Uemura R, Higuchi H.	Vet Res	10.1186/s13567-025-01468-1	2025
	Extracellular vesicles of minimalistic Mollicutes as mediators of immune modulation and horizontal gene transfer.	Wagner TM, Torres-Puig S, Yimthin T, Irobalieva RN, Heller M, Kaessmeyer S, Demoulins T, Jores J.	Commun Biol	10.1038/s42003-025-08099-4	2025
	Mycoplasma bovis 5'-nucleotidase is a virulence factor conferring mammary fitness in bovine mastitis.	Gelgie AE, Schneider P, Citti C, Dordet-Frisoni E, Gillespie BE, Almeida RA, Agga GE, Amoah YS, Shpigel NY, Kerro Dego O, Lysnyansky I.	PLoS Pathog	10.1371/journal.ppat.1012628	2024
Enzymology	MALDI-TOF mass spectrometry and high-resolution melting PCR for the identification of Mycoplasma bovis isolates.	McDaniel AJ, Derscheid RJ.	BMC Vet Res	10.1186/s12917-021-02870-5	2021
	Immune profiling of experimental murine mastitis reveals conserved response to mammary pathogenic Escherichia coli, Mycoplasma bovis, and Streptococcus uberis.	Schneider P, Salamon H, Weizmann N, Nissim-Eliraz E, Lysnyansky I, Shpigel NY.	Front Microbiol	10.3389/fmicb.2023.1126896	2023
Metabolism	Minimalistic mycoplasmas harbor different functional toxin-antitoxin systems.	Hill V, Akarsu H, Barbarroja RS, Cippa VL, Kuhnert P, Heller M, Falquet L, Heller M, Stoffel MH, Labroussaa F, Jores J.	PLoS Genet	10.1371/journal.pgen.1009365	2021
Metabolism	Immunoproteomic identification of MbovP579, a promising diagnostic biomarker for serological detection of Mycoplasma bovis infection.	Khan FA, Faisal M, Chao J, Liu K, Chen X, Zhao G, Menghwar H, Zhang H, Zhu X, Rasheed MA, He C, Hu C, Chen Y, Baranowski E, Chen H, Guo A.	Oncotarget	10.18632/oncotarget.9799	2016
	The addition of Lactobacillus spp. negatively affects Mycoplasma bovis viability in bovine cervical mucus.	Garcia-Galan A, De la Fe C, Gomis J, Bataller E, Sanchez A, Quereda JJ, Garcia-Rosello E, Gomez-Martin A	BMC Vet Res	10.1186/s12917-020-02454-9	2020
	The Addition of Lactobacillus spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of Mycoplasma bovis in Diluted Bovine Semen.	Garcia-Galan A, Gomez-Martin A, Bataller E, Gomis J, Sanchez A, Gadea J, Vieira LA, Garcia-Rosello E, De la Fe C	Animals (Basel)	10.3390/ani10050837	2020
Enzymology	Molecular epidemiological analysis of Mycoplasma bovis isolates from the Pennsylvania Animal Diagnostic Laboratory showing genetic diversity.	Soehnlen MK, Kariyawasam S, Lumadue JA, Pierre TA, Wolfgang DR, Jayarao BM	J Dairy Sci	10.3168/jds.2010-3967	2011
Genetics	Complete genome sequence of Mycoplasma bovis type strain PG45 (ATCC 25523).	Wise KS, Calcutt MJ, Foecking MF, Roske K, Madupu R, Methe BA	Infect Immun	10.1128/IAI.00726-10	2010
Enzymology	Determination of Mycoplasma bovis susceptibilities against six antimicrobial agents using the E test method.	Francoz D, Fortin M, Fecteau G, Messier S	Vet Microbiol	10.1016/j.vetmic.2004.10.006	2004
Genetics	ISMmy1, a novel insertion sequence of Mycoplasma mycoides subsp. mycoides small colony type.	Westberg J, Persson A, Pettersson B, Uhlen M, Johansson KE	FEMS Microbiol Lett	10.1111/j.1574-6968.2002.tb11083.x	2002
	The effect of Mycoplasma bovis on fertilization processes in vitro with bull spermatozoa and zona-free hamster oocytes.	Eaglesome MD, Garcia MM	Vet Microbiol	10.1016/0378-1135(90)90004-f	1990

References

#16514	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22781
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Mycoplasmopsis bovis (DSM 22781, ATCC 25523, NCTC 10131)

Name and taxonomic classification

Morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

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