Name: Mycobacterium neumannii 2409
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8246

Type strain:

Species: Mycobacterium neumannii

Strain Designation: 2409

Culture col. no.: DSM 43532, CECT 8766, SN 1904

Strain history: <- I. Tarnok, SN 1904 <- Hauduroy, 2409

NCBI tax ID(s): 2048551 (species)

Section

Mycobacterium neumannii 2409 is a facultative anaerobe, mesophilic, Gram-positive human pathogen of the family Mycobacteriaceae.

Gram-positive
facultative anaerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	25-08-2022 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Actinomycetota
	Class	Actinomycetes
	Order	Mycobacteriales
	Family	Mycobacteriaceae
	Genus	Mycobacterium
	Species	Mycobacterium neumannii
	Full Scientific Name (PNU)	Mycobacterium neumannii Nouioui et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65228]

Gram stain

positive

[Ref.: #65228]	Colony color	yellow-orange
[Ref.: #65228]	Incubation period	5 days
[Ref.: #65228]	Cultivation medium used	Middelbrook 7H10

Multimedia content

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Caption:

Medium 645 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

Middelbrook 7H10

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Culture medium growth

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Culture medium

proteose peptone-meat extract-glycerol agar

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Culture medium growth

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Culture medium

glucose-yeast extract-malt extract

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Culture medium growth

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Culture medium

tryptic soy agar

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Culture medium growth

		Temperature
[Ref.: #11120]	growth	28 °C
[Ref.: #65228]	growth	28-37 °C
[Ref.: #65228]	optimum	37 °C

[Ref.: #11120]	Temperature range	mesophilic
[Ref.: #65228]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65228]			growth	7

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

facultative anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65228]		NaCl		growth	0-8	%(w/v)

[Ref.: #65228]	Observation	Meso-diaminopimelic acid, arabinose, galactose, glucose and ribose are present in the whole-organism hydrolysates. The predominant menaquinone is MK-9(H2).
[Ref.: #65228]	Observation	Contains dicarboxy mycolic acids with 61–64 carbon atoms and keto-mycolates with 78-83 carbon atoms.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65228]	(S)-lactate ChEBI	-	assimilation
[Ref.: #65228]	2-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #65228]	2-oxobutanoate ChEBI	-	assimilation
[Ref.: #65228]	2-oxoglutarate ChEBI	+	assimilation
[Ref.: #65228]	3-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #65228]	3-O-methyl-D-glucose ChEBI	-	assimilation
[Ref.: #65228]	4-hydroxyphenylacetic acid ChEBI	-	assimilation
[Ref.: #65228]	acetate ChEBI	+	assimilation
[Ref.: #65228]	acetoacetate ChEBI	+	assimilation
[Ref.: #65228]	beta-gentiobiose ChEBI	-	assimilation
[Ref.: #65228]	bromosuccinate ChEBI	-	assimilation
[Ref.: #65228]	cellobiose ChEBI	-	assimilation
[Ref.: #65228]	citrate ChEBI	+	assimilation
[Ref.: #65228]	D-arabitol ChEBI	+	assimilation
[Ref.: #65228]	D-aspartate ChEBI	-	assimilation
[Ref.: #65228]	D-fructose ChEBI	+	assimilation
[Ref.: #65228]	D-fructose 6-phosphate ChEBI	+	assimilation
[Ref.: #65228]	D-fucose ChEBI	-	assimilation
[Ref.: #65228]	D-galactose ChEBI	-	assimilation
[Ref.: #65228]	D-galacturonic acid ChEBI	-	assimilation
[Ref.: #65228]	D-glucarate ChEBI	-	assimilation
[Ref.: #65228]	D-gluconate ChEBI	+	assimilation
[Ref.: #65228]	D-glucose ChEBI	+	assimilation
[Ref.: #65228]	D-glucose 6-phosphate ChEBI	-	assimilation
[Ref.: #65228]	D-glucuronate ChEBI	-	assimilation
[Ref.: #65228]	D-malate ChEBI	-	assimilation
[Ref.: #65228]	D-mannitol ChEBI	+	assimilation
[Ref.: #65228]	D-mannose ChEBI	+	assimilation
[Ref.: #65228]	D-serine ChEBI	+	assimilation
[Ref.: #65228]	D-sorbitol ChEBI	+	assimilation
[Ref.: #65228]	decanoate ChEBI	+	assimilation
[Ref.: #65228]	dextrin ChEBI	+	assimilation
[Ref.: #65228]	galactarate ChEBI	-	assimilation
[Ref.: #65228]	gamma-aminobutyric acid ChEBI	+	assimilation
[Ref.: #65228]	gelatin ChEBI	-	assimilation
[Ref.: #65228]	glucuronamide ChEBI	+	assimilation
[Ref.: #65228]	glycerol ChEBI	+	assimilation
[Ref.: #65228]	glycine-proline ChEBI	-	assimilation
[Ref.: #65228]	inosine ChEBI	-	assimilation
[Ref.: #65228]	L-alanine ChEBI	-	assimilation
[Ref.: #65228]	L-arginine ChEBI	-	assimilation
[Ref.: #65228]	L-aspartate ChEBI	-	assimilation
[Ref.: #65228]	L-fucose ChEBI	-	assimilation
[Ref.: #65228]	L-galactonic acid gamma-lactone ChEBI	-	assimilation
[Ref.: #65228]	L-glutamate ChEBI	+	assimilation
[Ref.: #65228]	L-histidine ChEBI	-	assimilation
[Ref.: #65228]	L-malate ChEBI	-	assimilation
[Ref.: #65228]	L-pyroglutamic acid ChEBI	-	assimilation
[Ref.: #65228]	L-rhamnose ChEBI	-	assimilation
[Ref.: #65228]	L-serine ChEBI	-	assimilation
[Ref.: #65228]	lactose ChEBI	-	assimilation
[Ref.: #65228]	maltose ChEBI	-	assimilation
[Ref.: #65228]	melibiose ChEBI	-	assimilation
[Ref.: #65228]	methyl (R)-lactate ChEBI	-	assimilation
[Ref.: #65228]	methyl beta-D-glucopyranoside ChEBI	-	assimilation
[Ref.: #65228]	methyl pyruvate ChEBI	+	assimilation
[Ref.: #65228]	myo-inositol ChEBI	-	assimilation
[Ref.: #65228]	N-acetyl-beta-D-mannosamine ChEBI	-	assimilation
[Ref.: #65228]	N-acetylgalactosamine ChEBI	-	assimilation
[Ref.: #65228]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #65228]	n-acetylneuraminate ChEBI	-	assimilation
[Ref.: #65228]	nitrate ChEBI	-	reduction
[Ref.: #65228]	pectin ChEBI	-	assimilation
[Ref.: #65228]	propionate ChEBI	+	assimilation
[Ref.: #65228]	quinate ChEBI	-	assimilation
[Ref.: #65228]	raffinose ChEBI	-	assimilation
[Ref.: #65228]	salicin ChEBI	+	assimilation
[Ref.: #65228]	stachyose ChEBI	-	assimilation
[Ref.: #65228]	sucrose ChEBI	-	assimilation
[Ref.: #65228]	trehalose ChEBI	+	assimilation
[Ref.: #65228]	turanose ChEBI	-	assimilation
[Ref.: #65228]	tween 40 ChEBI	-	degradation
[Ref.: #65228]	tween 80 ChEBI	+	degradation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #65228]	1 % sodium lactate		yes
[Ref.: #65228]	aztreonam ChEBI		yes
[Ref.: #65228]	fusidate ChEBI	yes
[Ref.: #65228]	guanidinium chloride ChEBI		yes
[Ref.: #65228]	lincomycin ChEBI	yes
[Ref.: #65228]	lithium chloride ChEBI		yes
[Ref.: #65228]	minocycline ChEBI	yes
[Ref.: #65228]	nalidixic acid ChEBI		yes
[Ref.: #65228]	niaproof ChEBI	yes
[Ref.: #65228]	potassium tellurite ChEBI		yes
[Ref.: #65228]	rifamycin sv ChEBI		yes
[Ref.: #65228]	sodium bromate ChEBI		yes
[Ref.: #65228]	sodium chloride ChEBI		yes
[Ref.: #65228]	sodium formate ChEBI		yes
[Ref.: #65228]	tetrazolium blue ChEBI		yes
[Ref.: #65228]	tetrazolium violet ChEBI		yes
[Ref.: #65228]	troleandomycin ChEBI	yes
[Ref.: #65228]	vancomycin ChEBI		yes
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #65228]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65228]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65228]	arylsulfatase	+	3.1.6.1
[Ref.: #65228]	beta-glucosidase	-	3.2.1.21
[Ref.: #65228]	catalase	+	1.11.1.6
[Ref.: #65228]	esterase (C 4)	+
[Ref.: #65228]	urease	+	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

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Fatty acid	Percentage	ECL
C16:0	25.2
unknown	22	19
C17:1ω7c / C17:1ω6c	15.4
C18:1ω9c	8.9
C16:1ω6c	8.7
C10Me-C18:0	5.6
C18:0	4.7
C14:0	3.7
C18:2ω6,9c	1.4

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11120]	Pathogenicity (human)	yes, in single cases
[Ref.: #11120]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65228]	16S rRNA gene sequence	KY933786		GenBank
[Ref.: #11120]	Mycobacterium neumannii strain CECT 8766 16S ribosomal RNA gene, partial sequence	KY933299	1533	ENA	2048551 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mycobacterium neumannii CECT 8766	GCA_002245615	scaffold	GenBank	2048551 tax ID	*
[Ref.: #66792]	Mycobacterium sp. CECT 8766	2015071.3	wgs	PATRIC	2048551 tax ID	*

[Ref.: #11120]	GC-content	66.8 mol%	sequence analysis
[Ref.: #65228]	GC-content	66.8 mol%	genome sequence analysis

Availability in culture collections External links

[Ref.: #11120]

Culture collection no.

DSM 43532, CECT 8766, SN 1904

[Ref.: #20218]

Associated Passport(s) in StrainInfo

168471

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov.	Nouioui I, Sangal V, Carro L, Teramoto K, Jando M, Montero-Calasanz MDC, Igual JM, Sutcliffe I, Goodfellow M, Klenk HP	Int J Syst Evol Microbiol	10.1099/ijsem.0.002350	2017	*

References

#11120

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43532

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#65228

Imen Nouioui, Vartul Sangal, Lorena Carro, Kanae Teramoto, Marlen Jando, Maria del Carmen Montero-Calasanz, José Mariano Igual, Iain Sutcliffe, Michael Goodfellow, Hans-Peter Klenk: Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov.. IJSEM 67: 4948 - 4955 2017 ( DOI 10.1099/ijsem.0.002350 , PubMed 29058645 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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