Name: Mycobacterium neumannii 2409
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 8246

Type strain:

Species: Mycobacterium neumannii

Strain Designation: 2409

Culture col. no.: DSM 43532, CECT 8766, SN 1904

Strain history: <- I. Tarnok, SN 1904 <- Hauduroy, 2409

NCBI tax ID(s): 2048551 (species)

SI-ID 50329

DSM 43532

Special Collections

Section

Mycobacterium neumannii 2409 is a facultative anaerobe, Gram-positive human pathogen of the family Mycobacteriaceae.

Gram-positive
facultative anaerobe
human pathogen
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium neumannii**
	Full Scientific Name (LPSN)	Mycobacterium neumannii Nouioui et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65228]	Gram stain	positive

[Ref.: #65228]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %90

[Ref.: #65228]	Colony color	yellow-orange
[Ref.: #65228]	Incubation period	5 days
[Ref.: #65228]	Cultivation medium used	Middelbrook 7H10

Multimedia content

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Caption:

Medium 645 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

Middelbrook 7H10

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Culture medium growth

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Culture medium

proteose peptone-meat extract-glycerol agar

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Culture medium growth

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Culture medium

glucose-yeast extract-malt extract

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Culture medium growth

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Culture medium

tryptic soy agar

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Culture medium growth

		Temperature
[Ref.: #11120]	growth	28 °C
[Ref.: #65228]	growth	28-37 °C
[Ref.: #65228]	optimum	37 °C

	pH	Kind of pH		pH
[Ref.: #65228]			growth	7

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

facultative anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65228]		NaCl		growth	0-8	%(w/v)

[Ref.: #65228]	Observation	Meso-diaminopimelic acid, arabinose, galactose, glucose and ribose are present in the whole-organism hydrolysates. The predominant menaquinone is MK-9(H2).
[Ref.: #65228]	Observation	Contains dicarboxy mycolic acids with 61–64 carbon atoms and keto-mycolates with 78-83 carbon atoms.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65228]	(S)-lactate ChEBI	-	assimilation
[Ref.: #65228]	2-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #65228]	2-oxobutanoate ChEBI	-	assimilation
[Ref.: #65228]	2-oxoglutarate ChEBI	+	assimilation
[Ref.: #65228]	3-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #65228]	3-O-methyl-D-glucose ChEBI	-	assimilation
[Ref.: #65228]	4-hydroxyphenylacetic acid ChEBI	-	assimilation
[Ref.: #65228]	acetate ChEBI	+	assimilation
[Ref.: #65228]	acetoacetate ChEBI	+	assimilation
[Ref.: #65228]	beta-gentiobiose ChEBI	-	assimilation
[Ref.: #65228]	bromosuccinate ChEBI	-	assimilation
[Ref.: #65228]	cellobiose ChEBI	-	assimilation
[Ref.: #65228]	citrate ChEBI	+	assimilation
[Ref.: #65228]	D-arabitol ChEBI	+	assimilation
[Ref.: #65228]	D-aspartate ChEBI	-	assimilation
[Ref.: #65228]	D-fructose ChEBI	+	assimilation
[Ref.: #65228]	D-fructose 6-phosphate ChEBI	+	assimilation
[Ref.: #65228]	D-fucose ChEBI	-	assimilation
[Ref.: #65228]	D-galactose ChEBI	-	assimilation
[Ref.: #65228]	D-galacturonic acid ChEBI	-	assimilation
[Ref.: #65228]	D-glucarate ChEBI	-	assimilation
[Ref.: #65228]	D-gluconate ChEBI	+	assimilation
[Ref.: #65228]	D-glucose ChEBI	+	assimilation
[Ref.: #65228]	D-glucose 6-phosphate ChEBI	-	assimilation
[Ref.: #65228]	D-glucuronate ChEBI	-	assimilation
[Ref.: #65228]	D-malate ChEBI	-	assimilation
[Ref.: #65228]	D-mannitol ChEBI	+	assimilation
[Ref.: #65228]	D-mannose ChEBI	+	assimilation
[Ref.: #65228]	D-serine ChEBI	+	assimilation
[Ref.: #65228]	D-sorbitol ChEBI	+	assimilation
[Ref.: #65228]	decanoate ChEBI	+	assimilation
[Ref.: #65228]	dextrin ChEBI	+	assimilation
[Ref.: #65228]	galactarate ChEBI	-	assimilation
[Ref.: #65228]	gamma-aminobutyric acid ChEBI	+	assimilation
[Ref.: #65228]	gelatin ChEBI	-	assimilation
[Ref.: #65228]	glucuronamide ChEBI	+	assimilation
[Ref.: #65228]	glycerol ChEBI	+	assimilation
[Ref.: #65228]	glycine-proline ChEBI	-	assimilation
[Ref.: #65228]	inosine ChEBI	-	assimilation
[Ref.: #65228]	L-alanine ChEBI	-	assimilation
[Ref.: #65228]	L-arginine ChEBI	-	assimilation
[Ref.: #65228]	L-aspartate ChEBI	-	assimilation
[Ref.: #65228]	L-fucose ChEBI	-	assimilation
[Ref.: #65228]	L-galactonic acid gamma-lactone ChEBI	-	assimilation
[Ref.: #65228]	L-glutamate ChEBI	+	assimilation
[Ref.: #65228]	L-histidine ChEBI	-	assimilation
[Ref.: #65228]	L-malate ChEBI	-	assimilation
[Ref.: #65228]	L-pyroglutamic acid ChEBI	-	assimilation
[Ref.: #65228]	L-rhamnose ChEBI	-	assimilation
[Ref.: #65228]	L-serine ChEBI	-	assimilation
[Ref.: #65228]	lactose ChEBI	-	assimilation
[Ref.: #65228]	maltose ChEBI	-	assimilation
[Ref.: #65228]	melibiose ChEBI	-	assimilation
[Ref.: #65228]	methyl (R)-lactate ChEBI	-	assimilation
[Ref.: #65228]	methyl beta-D-glucopyranoside ChEBI	-	assimilation
[Ref.: #65228]	methyl pyruvate ChEBI	+	assimilation
[Ref.: #65228]	myo-inositol ChEBI	-	assimilation
[Ref.: #65228]	N-acetyl-beta-D-mannosamine ChEBI	-	assimilation
[Ref.: #65228]	N-acetylgalactosamine ChEBI	-	assimilation
[Ref.: #65228]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #65228]	n-acetylneuraminate ChEBI	-	assimilation
[Ref.: #65228]	nitrate ChEBI	-	reduction
[Ref.: #65228]	pectin ChEBI	-	assimilation
[Ref.: #65228]	propionate ChEBI	+	assimilation
[Ref.: #65228]	quinate ChEBI	-	assimilation
[Ref.: #65228]	raffinose ChEBI	-	assimilation
[Ref.: #65228]	salicin ChEBI	+	assimilation
[Ref.: #65228]	stachyose ChEBI	-	assimilation
[Ref.: #65228]	sucrose ChEBI	-	assimilation
[Ref.: #65228]	trehalose ChEBI	+	assimilation
[Ref.: #65228]	turanose ChEBI	-	assimilation
[Ref.: #65228]	tween 40 ChEBI	-	degradation
[Ref.: #65228]	tween 80 ChEBI	+	degradation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #65228]	1 % sodium lactate		yes
[Ref.: #65228]	aztreonam ChEBI		yes
[Ref.: #65228]	fusidate ChEBI	yes
[Ref.: #65228]	guanidinium chloride ChEBI		yes
[Ref.: #65228]	lincomycin ChEBI	yes
[Ref.: #65228]	lithium chloride ChEBI		yes
[Ref.: #65228]	minocycline ChEBI	yes
[Ref.: #65228]	nalidixic acid ChEBI		yes
[Ref.: #65228]	niaproof ChEBI	yes
[Ref.: #65228]	potassium tellurite ChEBI		yes
[Ref.: #65228]	rifamycin sv ChEBI		yes
[Ref.: #65228]	sodium bromate ChEBI		yes
[Ref.: #65228]	sodium chloride ChEBI		yes
[Ref.: #65228]	sodium formate ChEBI		yes
[Ref.: #65228]	tetrazolium blue ChEBI		yes
[Ref.: #65228]	tetrazolium violet ChEBI		yes
[Ref.: #65228]	troleandomycin ChEBI	yes
[Ref.: #65228]	vancomycin ChEBI		yes
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #65228]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65228]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65228]	arylsulfatase	+	3.1.6.1
[Ref.: #65228]	beta-glucosidase	-	3.2.1.21
[Ref.: #65228]	catalase	+	1.11.1.6
[Ref.: #65228]	esterase (C 4)	+
[Ref.: #65228]	urease	+	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65228]

Fatty acid	Percentage	ECL
C16:0	25.2
unknown	22	19
C17:1ω7c / C17:1ω6c	15.4
C18:1ω9c	8.9
C16:1ω6c	8.7
C10Me-C18:0	5.6
C18:0	4.7
C14:0	3.7
C18:2ω6,9c	1.4

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11120]	Pathogenicity (human)	yes, in single cases
[Ref.: #11120]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11120]	Mycobacterium neumannii strain CECT 8766 16S ribosomal RNA gene, partial sequence	KY933299	1533	GenBank ENA	2048551 tax ID
[Ref.: #65228]	Mycobacterium lehmannii strain CECT 8763 heat shock protein 65 KDa gene, complete cds	KY933786		GenBank ENA

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mycobacterium neumannii CECT 8766	GCA_002245615	scaffold	GenBank ENA	2048551 tax ID	*
[Ref.: #66792]	Mycobacterium sp. CECT 8766	2015071.3	wgs	PATRIC	2048551 tax ID	*

[Ref.: #11120]	GC-content	66.8 mol%	sequence analysis
[Ref.: #65228]	GC-content	66.8 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Mycobacterium neumannii CECT 8766 NCBI: GCA_002245615.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	86	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycobacterium neumannii CECT 8766 NCBI: GCA_002245615.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	89.951	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	97.991	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	84.47	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	60.567	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	90	no

Availability in culture collections External links

[Ref.: #11120]

Culture collection no.

DSM 43532, CECT 8766, SN 1904

[Ref.: #77648]

SI-ID 50329

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov.	Nouioui I, Sangal V, Carro L, Teramoto K, Jando M, Montero-Calasanz MDC, Igual JM, Sutcliffe I, Goodfellow M, Klenk HP	Int J Syst Evol Microbiol	10.1099/ijsem.0.002350	2017	*

References

#11120

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43532

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65228

Imen Nouioui, Vartul Sangal, Lorena Carro, Kanae Teramoto, Marlen Jando, Maria del Carmen Montero-Calasanz, José Mariano Igual, Iain Sutcliffe, Michael Goodfellow, Hans-Peter Klenk: Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov.. IJSEM 67: 4948 - 4955 2017 ( DOI 10.1099/ijsem.0.002350 , PubMed 29058645 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#77648

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID50329.1 )

* These data were automatically processed and therefore are not curated

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Mycobacterium neumannii CECT 8766

Mycobacterium neumannii CECT 8766

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