Strain identifier

BacDive ID: 8246

Type strain: Yes

Species: Mycobacterium neumannii

Strain Designation: 2409

Strain history: <- I. Tarnok, SN 1904 <- Hauduroy, 2409

NCBI tax ID(s): 2048551 (species)

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General

@ref: 11120

BacDive-ID: 8246

DSM-Number: 43532

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, human pathogen

description: Mycobacterium neumannii 2409 is a facultative anaerobe, mesophilic, Gram-positive human pathogen of the family Mycobacteriaceae.

NCBI tax id

  • NCBI tax id: 2048551
  • Matching level: species

strain history

  • @ref: 11120
  • history: <- I. Tarnok, SN 1904 <- Hauduroy, 2409

doi: 10.13145/bacdive8246.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium neumannii
  • full scientific name: Mycobacterium neumannii Nouioui et al. 2017

@ref: 11120

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium neumannii

full scientific name: Mycobacterium neumannii Nouioui et al. 2017

strain designation: 2409

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
65228positiveno
69480no94.84
69480positive100

colony morphology

  • @ref: 65228
  • colony color: yellow-orange
  • incubation period: 5 days
  • medium used: Middelbrook 7H10

multimedia

  • @ref: 11120
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43532.jpg
  • caption: Medium 645 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11120GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
11120MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
65228Middelbrook 7H10yes
65228proteose peptone-meat extract-glycerol agaryes
65228glucose-yeast extract-malt extractyes
65228tryptic soy agaryes

culture temp

@refgrowthtypetemperaturerange
11120positivegrowth28mesophilic
65228positivegrowth28-37mesophilic
65228positiveoptimum37mesophilic

culture pH

  • @ref: 65228
  • ability: positive
  • type: growth
  • pH: 7

Physiology and metabolism

oxygen tolerance

  • @ref: 65228
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.883

halophily

  • @ref: 65228
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-8 %(w/v)

observation

@refobservation
65228Meso-diaminopimelic acid, arabinose, galactose, glucose and ribose are present in the whole-organism hydrolysates. The predominant menaquinone is MK-9(H2).
65228Contains dicarboxy mycolic acids with 61–64 carbon atoms and keto-mycolates with 78-83 carbon atoms.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6522815588D-malate-assimilation
6522816651(S)-lactate-assimilation
6522815589L-malate-assimilation
65228167632-oxobutanoate-assimilation
65228309162-oxoglutarate+assimilation
65228370543-hydroxybutyrate+assimilation
65228739183-O-methyl-D-glucose-assimilation
65228181014-hydroxyphenylacetic acid-assimilation
6522830089acetate+assimilation
6522813705acetoacetate+assimilation
65228645522-hydroxybutyrate+assimilation
6522871422beta-gentiobiose-assimilation
6522873706bromosuccinate-assimilation
6522827689decanoate+assimilation
6522817057cellobiose-assimilation
6522816947citrate+assimilation
6522818333D-arabitol+assimilation
6522829990D-aspartate-assimilation
6522815824D-fructose+assimilation
6522878697D-fructose 6-phosphate+assimilation
6522828847D-fucose-assimilation
6522812936D-galactose-assimilation
6522818024D-galacturonic acid-assimilation
6522830612D-glucarate-assimilation
652288391D-gluconate+assimilation
6522817634D-glucose+assimilation
6522814314D-glucose 6-phosphate-assimilation
6522815748D-glucuronate-assimilation
6522816899D-mannitol+assimilation
6522816024D-mannose+assimilation
6522816523D-serine+assimilation
6522817924D-sorbitol+assimilation
6522832528turanose-assimilation
6522823652dextrin+assimilation
6522816537galactarate-assimilation
6522816865gamma-aminobutyric acid+assimilation
652285291gelatin-assimilation
6522832323glucuronamide+assimilation
6522817754glycerol+assimilation
6522870744glycine-proline-assimilation
6522817596inosine-assimilation
6522816977L-alanine-assimilation
6522816467L-arginine-assimilation
6522829991L-aspartate-assimilation
6522818287L-fucose-assimilation
6522817464L-galactonic acid gamma-lactone-assimilation
6522829985L-glutamate+assimilation
6522815971L-histidine-assimilation
6522818183L-pyroglutamic acid-assimilation
6522862345L-rhamnose-assimilation
6522817115L-serine-assimilation
6522817716lactose-assimilation
6522817306maltose-assimilation
6522828053melibiose-assimilation
6522874611methyl (R)-lactate-assimilation
65228320055methyl beta-D-glucopyranoside-assimilation
6522851850methyl pyruvate+assimilation
6522817268myo-inositol-assimilation
6522863154N-acetyl-beta-D-mannosamine-assimilation
6522828037N-acetylgalactosamine-assimilation
65228506227N-acetylglucosamine+assimilation
6522835418n-acetylneuraminate-assimilation
6522817632nitrate-reduction
6522817309pectin-assimilation
6522817272propionate+assimilation
6522826490quinate-assimilation
6522816634raffinose-assimilation
6522817814salicin+assimilation
6522817164stachyose-assimilation
6522817992sucrose-assimilation
6522827082trehalose+assimilation
6522853423tween 40-degradation
6522853426tween 80+degradation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistant
6522871321fusidateyesyes
652286472lincomycinyesyes
6522850694minocyclineyesyes
6522875273niaproofyesyes
6522845735troleandomycinyesyes
65228161680aztreonamyesyes
6522848607lithium chlorideyesyes
65228100147nalidixic acidyesyes
6522829673rifamycin svyesyes
6522875248potassium telluriteyesyes
6522862965sodium formateyesyes
652281 % sodium lactateyesyes
6522875229sodium bromateyesyes
6522832735guanidinium chlorideyesyes
6522875198tetrazolium blueyesyes
6522875193tetrazolium violetyesyes
6522828001vancomycinyesyes
6522826710sodium chlorideyesyes

enzymes

@refvalueactivityec
65228arylsulfatase+3.1.6.1
65228esterase (C 4)+
65228alkaline phosphatase+3.1.3.1
65228alpha-glucosidase-3.2.1.20
65228beta-glucosidase-3.2.1.21
65228catalase+1.11.1.6
65228urease+3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    65228C16:025.2
    65228C16:1ω6c8.7
    65228C18:04.7
    65228C18:1ω9c8.9
    65228C18:2ω6,9c1.4
    65228C10Me-C18:05.6
    65228C14:03.7
    65228C17:1ω7c / C17:1ω6c15.4
    65228unknown2219
  • type of FA analysis: whole cell analysis
  • incubation medium: MD7H10 broth
  • incubation temperature: 37
  • incubation time: 7
  • software version: Sherlock 4.5
  • library/peak naming table: Myco5
  • system: MIS MIDI

Safety information

risk assessment

  • @ref: 11120
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessiondatabaselengthNCBI tax ID
6522816S rRNA gene sequenceKY933786nuccore
11120Mycobacterium neumannii strain CECT 8766 16S ribosomal RNA gene, partial sequenceKY933299ena15332048551

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium neumannii CECT 8766GCA_002245615scaffoldncbi2048551
66792Mycobacterium sp. CECT 87662015071.3wgspatric2048551

GC content

@refGC-contentmethod
1112066.8sequence analysis
6522866.8genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno86no
flagellatedno98.722no
gram-positiveyes90.226yes
anaerobicno99.48no
aerobicyes86.913yes
halophileno86.483no
spore-formingno89.485no
glucose-utilyes86.492yes
thermophileno99.472no
motileno93.892no
glucose-fermentno91.356no

External links

@ref: 11120

culture collection no.: DSM 43532, CECT 8766, SN 1904

straininfo link

  • @ref: 77648
  • straininfo: 50329

literature

  • topic: Phylogeny
  • Pubmed-ID: 29058645
  • title: Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov.
  • authors: Nouioui I, Sangal V, Carro L, Teramoto K, Jando M, Montero-Calasanz MDC, Igual JM, Sutcliffe I, Goodfellow M, Klenk HP
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002350
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Multilocus Sequence Typing, Mycobacterium/*classification/isolation & purification, Mycolic Acids/chemistry, Nucleic Acid Hybridization, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11120Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43532)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43532
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
65228Imen Nouioui, Vartul Sangal, Lorena Carro, Kanae Teramoto, Marlen Jando, Maria del Carmen Montero-Calasanz, José Mariano Igual, Iain Sutcliffe, Michael Goodfellow, Hans-Peter Klenk10.1099/ijsem.0.002350Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov.IJSEM 67: 4948-4955 201729058645
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
77648Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID50329.1StrainInfo: A central database for resolving microbial strain identifiers