Rothia mucilaginosa (DSM 20746, ATCC 25296, CCM 2417)

Name: Rothia mucilaginosa (DSM 20746, ATCC 25296, CCM 2417)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7777

Type strain

Strain Designation 5762/67

Culture col. no. DSM 20746 ATCC 25296 CCM 2417 NCTC 10663 CCUG 20962 14 more

NCBI tax ID(s) 553201 43675

Special Collections DSMZ CCUG JCM CIP

History <- CCM; CCM 2417 <- T. Bergan; 5762/67 CCUG 20962 <-- CCM 2417 <-- T. Bergan 5762/67. CIP <- 1971, CCM <- 1967, T. Bergan: strain 5762/67 Micrococcus mucilaginosus

Links

Rothia mucilaginosa 5762/67 is a microaerophile, Gram-positive, coccus-shaped bacterium that was isolated from throat.

Gram-positive coccus-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 389300

ATCC 25296,CCM 2417,NCTC 10663,DSM 20746,CCUG 20962,NCDO 2360,CCUG 3707,NCIMB 702360,IFO 15673,JCM 10910,NBRC 15673,IAM 14815,CIP 71.14

@ref 20215

Last LPSN update 2026-02-09 11:19:33

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Micrococcaceae

Genus Rothia

Species Rothia mucilaginosa

Full scientific name Rothia mucilaginosa (Bergan and Kocur 1982 ex Migula 1900) Collins et al. 2000

Synonyms (1)

Stomatococcus mucilaginosus

BacDive ID	Other strains from **Rothia mucilaginosa** (66)	Type strain
7775	R. mucilaginosa 421/68, DSM 20445, CCM 2485, JCM 21309, IAM ...
7776	R. mucilaginosa 2849/68, DSM 20446, CCM 2486, JCM 21310, ...
7778	R. mucilaginosa ES1-209-1, DSM 30548
137535	R. mucilaginosa CIP 71.13
142598	R. mucilaginosa CCUG 13185
142867	R. mucilaginosa CCUG 15424 A
143348	R. mucilaginosa CCUG 18678
143591	R. mucilaginosa CCUG 20499
144586	R. mucilaginosa CCUG 25828
145126	R. mucilaginosa CCUG 27714 B
145444	R. mucilaginosa CCUG 28566
145798	R. mucilaginosa CCUG 29631
145836	R. mucilaginosa CCUG 29746
145855	R. mucilaginosa CCUG 29798
146073	R. mucilaginosa CCUG 30339
146361	R. mucilaginosa CCUG 31024 A
146454	R. mucilaginosa CCUG 31188
146455	R. mucilaginosa CCUG 31189
146575	R. mucilaginosa CCUG 31477
146629	R. mucilaginosa CCUG 31653
146642	R. mucilaginosa CCUG 31703
146656	R. mucilaginosa CCUG 31734
146684	R. mucilaginosa CCUG 31798
146805	R. mucilaginosa CCUG 32149
146997	R. mucilaginosa CCUG 32620
147076	R. mucilaginosa CCUG 32803
147187	R. mucilaginosa CCUG 33020
147391	R. mucilaginosa CCUG 33515
147806	R. mucilaginosa CCUG 34263
148049	R. mucilaginosa CCUG 34730
148213	R. mucilaginosa CCUG 35048
148524	R. mucilaginosa CCUG 35589
148730	R. mucilaginosa CCUG 35870
148762	R. mucilaginosa CCUG 35912
149003	R. mucilaginosa CCUG 36703
149138	R. mucilaginosa CCUG 36920
149258	R. mucilaginosa CCUG 37354
149262	R. mucilaginosa CCUG 37373
149296	R. mucilaginosa CCUG 37443
149351	R. mucilaginosa CCUG 37563
149506	R. mucilaginosa CCUG 37871
149895	R. mucilaginosa CCUG 38635
150726	R. mucilaginosa CCUG 42088
150770	R. mucilaginosa CCUG 42258
150780	R. mucilaginosa CCUG 42305
150866	R. mucilaginosa CCUG 42589
151112	R. mucilaginosa CCUG 43297
151307	R. mucilaginosa CCUG 43707 B
151311	R. mucilaginosa CCUG 43722
151813	R. mucilaginosa CCUG 44966
152008	R. mucilaginosa CCUG 45514
152121	R. mucilaginosa CCUG 45837
152422	R. mucilaginosa CCUG 46756
152728	R. mucilaginosa CCUG 47492
153060	R. mucilaginosa CCUG 48398
153062	R. mucilaginosa CCUG 48402
153285	R. mucilaginosa CCUG 48997
154137	R. mucilaginosa CCUG 52532
154276	R. mucilaginosa CCUG 52952
154787	R. mucilaginosa CCUG 55182 A
154842	R. mucilaginosa CCUG 55378
154851	R. mucilaginosa CCUG 55400
155072	R. mucilaginosa CCUG 56385
173306	R. mucilaginosa CIMB 04-9299, CRBIP17.108
173307	R. mucilaginosa CIMB 04-7239, CRBIP17.109
174655	R. mucilaginosa 116788, DSM 116788

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
121156	positive	coccus-shaped
125439	positive		99.1
125439			96.2
125438			94

Colony morphology

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
9055	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
9055	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
37615	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
121156	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
9055	positive	growth	37
9055	positive	growth	30
37615	positive	growth	30
47240	positive	growth	37
67770	positive	growth	30
121156	positive	growth	30-41
121156	negative	growth	10
121156	negative	growth	22
121156	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
47240	microaerophile
121156	facultative anaerobe

Murein

@ref	Murein short key	Type
9055	A11.04	A3alpha L-Lys-L-Ala

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68381	40585 ChEBI	alpha-cyclodextrin	-	builds acid from	from API rID32STR
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68381	29016 ChEBI	arginine	-	hydrolysis	from API rID32STR
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68381	18333 ChEBI	D-arabitol	-	builds acid from	from API rID32STR
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68379	17634 ChEBI	D-glucose	+	fermentation	from API Coryne
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68381	16899 ChEBI	D-mannitol	-	builds acid from	from API rID32STR
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68381	16988 ChEBI	D-ribose	-	builds acid from	from API rID32STR
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68381	16443 ChEBI	D-tagatose	-	builds acid from	from API rID32STR
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68381	28087 ChEBI	glycogen	-	builds acid from	from API rID32STR
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68381	606565 ChEBI	hippurate	-	hydrolysis	from API rID32STR
121156	606565 ChEBI	hippurate	+	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68381	30849 ChEBI	L-arabinose	-	builds acid from	from API rID32STR
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68381	17716 ChEBI	lactose	-	builds acid from	from API rID32STR
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68379	17306 ChEBI	maltose	+	fermentation	from API Coryne
68381	17306 ChEBI	maltose	+	builds acid from	from API rID32STR
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68381	6731 ChEBI	melezitose	+	builds acid from	from API rID32STR
68371	6731 ChEBI	melezitose	+	builds acid from	from API 50CH acid
68381	28053 ChEBI	melibiose	-	builds acid from	from API rID32STR
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	+	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68381	320055 ChEBI	methyl beta-D-glucopyranoside	+	builds acid from	from API rID32STR
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
68379	17632 ChEBI	nitrate	+	reduction	from API Coryne
121156	17632 ChEBI	nitrate	+	reduction
121156	16301 ChEBI	nitrite	+	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68381	27941 ChEBI	pullulan	-	builds acid from	from API rID32STR
68381	16634 ChEBI	raffinose	-	builds acid from	from API rID32STR
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68381	30911 ChEBI	sorbitol	-	builds acid from	from API rID32STR
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
68381	17992 ChEBI	sucrose	+	builds acid from	from API rID32STR
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68381	27082 ChEBI	trehalose	+	builds acid from	from API rID32STR
68371	32528 ChEBI	turanose	+	builds acid from	from API 50CH acid
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
68381	16199 ChEBI	urea	-	hydrolysis	from API rID32STR
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68381	15688 ChEBI	acetoin		from API rID32STR
121156	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
68381	15688 ChEBI	acetoin	+	from API rID32STR
121156	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68381	Alanyl-Phenylalanyl-Proline arylamidase	+		from API rID32STR
121156	alcohol dehydrogenase	-	1.1.1.1
68381	alkaline phosphatase	-	3.1.3.1	from API rID32STR
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68381	alpha-galactosidase	-	3.2.1.22	from API rID32STR
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
121156	amylase	-
68381	arginine dihydrolase	-	3.5.3.6	from API rID32STR
68381	beta-galactosidase	-	3.2.1.23	from API rID32STR
68382	beta-galactosidase	-	3.2.1.23	from API zym
121156	beta-galactosidase	-	3.2.1.23
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68381	beta-glucuronidase	-	3.2.1.31	from API rID32STR
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
68381	beta-mannosidase	-	3.2.1.25	from API rID32STR
121156	caseinase	-	3.4.21.50
121156	catalase	-	1.11.1.6
68379	catalase	-	1.11.1.6	from API Coryne
121156	coagulase	-
68382	cystine arylamidase	-	3.4.11.3	from API zym
121156	DNase	-
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
121156	gamma-glutamyltransferase	-	2.3.2.2
121156	gelatinase	-
68379	gelatinase	+		from API Coryne
121156	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
121156	lipase	-
68382	lipase (C 14)	-		from API zym
121156	lysine decarboxylase	-	4.1.1.18
68381	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32STR
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
121156	ornithine decarboxylase	-	4.1.1.17
121156	oxidase	-
121156	phenylalanine ammonia-lyase	-	4.3.1.24
121156	protease	-
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68381	pyrrolidonyl arylamidase	+	3.4.19.3	from API rID32STR
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
121156	tween esterase	-
68381	urease	-	3.5.1.5	from API rID32STR
121156	urease	-	3.5.1.5
68379	urease	-	3.5.1.5	from API Coryne
68382	valine arylamidase	-		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
9055	+	-	+	-	-	-	+	-	+	-	+	-	+	-	-	-	+	-	+	-	-

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121156	-	-	-	-	-	+	-	-	-	-	+	-	-	-	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
121156	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	+	-	-	-	+/-	-	-	+	-	-	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-	+/-

API rID32STR

@ref	ADH (Arg)	beta GLU	beta GAR	beta GUR	alpha GAL	PAL	RIB	MAN	SOR	LAC	TRE	RAF	SAC	LARA	DARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	HIP	GLYG	PUL	MAL	MEL	MLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	TAG	beta MAN	URE
9055	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-	-	not determinedn.d.	+	-	+	-	+	-	-	-	+	-	+	+	-	-	-
9055	-	+	-	-	-	-	-	-	-	-	+	-	+	-	-	-	+	+	-	+	-	-	-	-	-	+	-	+	+	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
121156	not determinedn.d.	+	-	-	-	-	-	-	+	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host Body-Site	#Oral cavity and airways	#Throat

Isolation

@ref	Sample type	Sampling date	Country	Country ISO 3 Code	Continent	Isolation date
9055	throat
47240	Human pharynx	1967	Norway	NOR	Europe
67770	Human throat		Norway	NOR	Europe
121156	Human, Throat		Norway	NOR	Europe	1967

Taxonmaps

69479

Global distribution of 16S sequence X87758 (>99% sequence identity) for Rothia mucilaginosa from Microbeatlas

Aquatic Samples	6900
Soil Samples	4381
Animal Samples	205395
Plant Samples	2095
Total Samples	218771

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
9055	2	Risk group (German classification) According to TRBA 466
121156	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM17561v1 assembly for Rothia mucilaginosa ATCC 25296	contig	GCA_000175615	553201.3	645058800	553201	74.37

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	M.mucilaginosus 16S rRNA gene	X87758	1467	43675
20218	S.mucilaginosus 16S rRNA gene	X95483	1435	43675
124043	Rothia mucilaginosa DSM 20746 gene for 16S rRNA, partial sequence.	LC752357	570	43675

GC content

@ref	GC-content (mol%)	Method
9055	59.0
67770	59	thermal denaturation, midpoint method (Tm)
67770	59.5	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Rothia mucilaginosa ATCC 25296
NCBI: GCA_000175615

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	87.30	no
125439	motility	BacteriaNetⓘ	no	96.20	no
125439	gram_stain	BacteriaNetⓘ	positive	99.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	82.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Rothia mucilaginosa ATCC 25296
PATRIC: 553201.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.35	no
125438	anaerobic	anaerobicⓘ	no	81.15	no
125438	spore-forming	spore-formingⓘ	no	84.18	no
125438	aerobic	aerobicⓘ	no	57.11	no
125438	thermophilic	thermophileⓘ	no	95.82	yes
125438	flagellated	motile2+ⓘ	no	94.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Titanium Dioxide Nanoparticles Induce Inhibitory Effects against Planktonic Cells and Biofilms of Human Oral Cavity Isolates of Rothia mucilaginosa, Georgenia sp. and Staphylococcus saprophyticus.	Fatima S, Ali K, Ahmed B, Al Kheraif AA, Syed A, Elgorban AM, Musarrat J, Lee J.	Pharmaceutics	10.3390/pharmaceutics13101564	2021
Genetics	Acetaldehyde production by Rothia mucilaginosa isolates from patients with oral leukoplakia.	Amer A, Whelan A, Al-Hebshi NN, Healy CM, Moran GP.	J Oral Microbiol	10.1080/20002297.2020.1743066	2020
Pathogenicity	Developing selective media for quantification of multispecies biofilms following antibiotic treatment.	Vandeplassche E, Coenye T, Crabbe A.	PLoS One	10.1371/journal.pone.0187540	2017
	Nitrate reduction capacity of the oral microbiota is impaired in periodontitis: potential implications for systemic nitric oxide availability.	Rosier BT, Johnston W, Carda-Dieguez M, Simpson A, Cabello-Yeves E, Piela K, Reilly R, Artacho A, Easton C, Burleigh M, Culshaw S, Mira A.	Int J Oral Sci	10.1038/s41368-023-00266-9	2024
	A Single Dose of Nitrate Increases Resilience Against Acidification Derived From Sugar Fermentation by the Oral Microbiome.	Rosier BT, Palazon C, Garcia-Esteban S, Artacho A, Galiana A, Mira A.	Front Cell Infect Microbiol	10.3389/fcimb.2021.692883	2021
Enzymology	Bacterial Interference With Lactate Dehydrogenase Assay Leads to an Underestimation of Cytotoxicity.	Van den Bossche S, Vandeplassche E, Ostyn L, Coenye T, Crabbe A.	Front Cell Infect Microbiol	10.3389/fcimb.2020.00494	2020
Pathogenicity	Host metabolites stimulate the bacterial proton motive force to enhance the activity of aminoglycoside antibiotics.	Crabbe A, Ostyn L, Staelens S, Rigauts C, Risseeuw M, Dhaenens M, Daled S, Van Acker H, Deforce D, Van Calenbergh S, Coenye T.	PLoS Pathog	10.1371/journal.ppat.1007697	2019
Genetics	Seven genome sequences of Rothia spp. isolated from human saliva.	Choi A, Pia L, Kaspar JR.	Microbiol Resour Announc	10.1128/mra.00465-25	2025
	Draft genome sequences of six Rothia mucilaginosa strains assembled from the human oral microbiome.	Saito D, Saito CPB, Cannavan FDS, Tsai SM.	Microbiol Resour Announc	10.1128/mra.00508-25	2025
	New Phage-Derived Antibacterial Enzyme PolaR Targeting Rothia spp.	Miernikiewicz P, Barylski J, Wilczak A, Dragos A, Rybicka I, Baldysz S, Szymczak A, Dogsa I, Rokush K, Harhala MA, Ciekot J, Ferenc S, Gnus J, Witkiewicz W, Dabrowska K.	Cells	10.3390/cells12151997	2023
Genetics	Genome-scale model of Rothia mucilaginosa predicts gene essentialities and reveals metabolic capabilities.	Leonidou N, Ostyn L, Coenye T, Crabbe A, Drager A.	Microbiol Spectr	10.1128/spectrum.04006-23	2024
Metabolism	Commensal Bacterium Rothia aeria Degrades and Detoxifies Gluten via a Highly Effective Subtilisin Enzyme.	Wei G, Darwish G, Oppenheim FG, Schuppan D, Helmerhorst EJ.	Nutrients	10.3390/nu12123724	2020
Enzymology	Effect of Rothia mucilaginosa enzymes on gliadin (gluten) structure, deamidation, and immunogenic epitopes relevant to celiac disease.	Tian N, Wei G, Schuppan D, Helmerhorst EJ.	Am J Physiol Gastrointest Liver Physiol	10.1152/ajpgi.00144.2014	2014
	Microbial signals in primary and metastatic brain tumors.	Morad G, Damania AV, Melendez B, Singh BB, Veguilla FJ, Soto RA, Hoballah YM, Sahasrabhojane PV, Wong MC, Ahmed MM, Rico RN, Lewis KN, Wani K, Shamsutdinova DD, Lazcano Segura RN, Ingram DR, Goethe EA, Day A, Flores II, McDaniel LK, Chelvanambi M, Johnson SB, Dimitriou F, Gupta P, Oberai S, Zal MA, Doss P, Jamal MA, Hayase E, Wathoo C, Norberg LM, Jenkins SL, Nass S, Gumin J, Long L, Yang J, Bradley GR, Bekal MP, Dono AG, Pichardo-Rojas PS, Andrewes SW, Ballester LY, Losh JS, Liang J, Huo L, Nielsen DC, Parker Kerrigan BC, Brastianos PK, Fowlkes NW, Chang CC, Jenq RR, Gomez-Manzano C, Huse JT, Davies MA, Lazar AJ, Bhat KP, Tandon N, Esquenazi Y, Peterson CB, Puduvalli VK, Lang FF, Johnston CD, Bullman S, Ajami NJ, Ferguson SD, Wargo JA.	Nat Med	10.1038/s41591-025-03957-4	2025
Metabolism	Identification of food-grade subtilisins as gluten-degrading enzymes to treat celiac disease.	Wei G, Tian N, Siezen R, Schuppan D, Helmerhorst EJ.	Am J Physiol Gastrointest Liver Physiol	10.1152/ajpgi.00185.2016	2016
Metabolism	Identification of Rothia bacteria as gluten-degrading natural colonizers of the upper gastro-intestinal tract.	Zamakhchari M, Wei G, Dewhirst F, Lee J, Schuppan D, Oppenheim FG, Helmerhorst EJ.	PLoS One	10.1371/journal.pone.0024455	2011
Genetics	Mechanistic model of Rothia mucilaginosa adaptation toward persistence in the CF lung, based on a genome reconstructed from metagenomic data.	Lim YW, Schmieder R, Haynes M, Furlan M, Matthews TD, Whiteson K, Poole SJ, Hayes CS, Low DA, Maughan H, Edwards R, Conrad D, Rohwer F.	PLoS One	10.1371/journal.pone.0064285	2013
Pathogenicity	Spatial Ecology of the Human Tongue Dorsum Microbiome.	Wilbert SA, Mark Welch JL, Borisy GG.	Cell Rep	10.1016/j.celrep.2020.02.097	2020
Metabolism	Bioorthogonal non-canonical amino acid tagging reveals translationally active subpopulations of the cystic fibrosis lung microbiota.	Valentini TD, Lucas SK, Binder KA, Cameron LC, Motl JA, Dunitz JM, Hunter RC.	Nat Commun	10.1038/s41467-020-16163-2	2020
	Alcohol-Derived Acetaldehyde Exposure in the Oral Cavity.	Stornetta A, Guidolin V, Balbo S.	Cancers (Basel)	10.3390/cancers10010020	2018
	Commensal Oral Rothia mucilaginosa Produces Enterobactin, a Metal-Chelating Siderophore.	Uranga CC, Arroyo P Jr, Duggan BM, Gerwick WH, Edlund A	mSystems	10.1128/mSystems.00161-20	2020
Phylogeny	Identification of disulphide stress-responsive extracytoplasmic function sigma factors in Rothia mucilaginosa.	Nambu T, Yamane K, Yamanaka T, Mashimo C, Maruyama H, Yoshida M, Hayashi H, Leung KP, Fukushima H	Arch Oral Biol	10.1016/j.archoralbio.2012.10.017	2013
Phylogeny	[Isolation and comparative analysis of glycolipid fractions in bifidobacteria].	Novik GI, Astapovich NI, Grzegorzewicz A, Gamian A	Mikrobiologiia		2005

References

#9055	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20746
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#37615	; Curators of the CIP;
#47240	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 20962
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68379	Automatically annotated from API Coryne .
#68381	Automatically annotated from API rID32STR .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121156	Collection of Institut Pasteur ; Curators of the CIP; CIP 71.14
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Rothia mucilaginosa (DSM 20746, ATCC 25296, CCM 2417)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Murein

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API coryne

API zym

API 50CHac

API rID32STR

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000175615

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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