Glutamicibacter uratoxydans (DSM 20647, ATCC 21749, U-23)

Name: Glutamicibacter uratoxydans (DSM 20647, ATCC 21749, U-23)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7568

Type strain

Strain Designation U-23

Culture col. no. DSM 20647 ATCC 21749 U-23 VKM Ac-1979 NCIMB 702282 10 more

NCBI tax ID(s) 43667

Special Collections DSMZ JCM KCTC CIP Wink compendium

Links

Glutamicibacter uratoxydans U-23 is an obligate aerobe, mesophilic, Gram-positive prokaryote that was isolated from humus soil.

Gram-positive motile rod-shaped obligate aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 8193

LMG 16220,ATCC 21749,CIP 102367,DSM 20647,IFO 15515,NCFB 2282,NCDO 2282,NCIMB 702282,VKM Ac-1979,KCTC 3482,JCM 11944,NBRC 15515,BCRC 14857,CCRC 14857,CGMCC 1.2821

@ref 20215

Last LPSN update 2025-12-16 09:48:37

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Micrococcaceae

Genus Glutamicibacter

Species Glutamicibacter uratoxydans

Full scientific name Glutamicibacter uratoxydans (Stackebrandt et al. 1984) Busse 2016

Synonyms (1)

Arthrobacter uratoxydans

BacDive ID	Other strains from **Glutamicibacter uratoxydans** (2)	Type strain
7569	G. uratoxydans U-125, DSM 20648, ATCC 21752, CIP 102366
102941	G. uratoxydans STI09742(IMET), IFO 14480, INMI 187, IMET ...

Morphology

Cell morphology

@ref	Gram stain	Cell shape
123737	positive	rod-shaped
125438
125438	positive
125439	negative

Colony morphology

@ref	Colony color	Incubation period	Medium used
18453	Colorless	10-14 days	ISP 2
18453	Colorless	10-14 days	ISP 3

Multicellular morphology

@ref	Forms multicellular complex	Medium name
18453		ISP 2
18453		ISP 3

Culture and growth conditions

Culture medium

@ref	Name	Composition
18453	ISP 2	Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18453	ISP 3	Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
41914	MEDIUM 3 - Columbia agar	Columbia agar (39.000 g);distilled water (1000.000 ml)
8867	CORYNEBACTERIUM AGAR (DSMZ Medium 53)	Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water
123737	CIP Medium 3
123737	CIP Medium 72

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8867	positive	growth	30	mesophilic
18453	positive	optimum	28	mesophilic
41914	positive	growth	25	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

123737

Oxygen toleranceobligate aerobe

Spore formation

@ref	Spore formation	Confidence
125439		99.3

Compound production

@ref	Compound
8867	uricase
67770	Uricase (US Pat. 3,767,533)

Halophily

@ref	Salt	Growth	Tested relation	Concentration
18453	NaCl	positive	maximum	7.5 %

Murein

@ref	Murein short key	Type
8867	A11.35	A4alpha L-Lys-L-Ala-L-Glu

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity
18453	22599 ChEBI	arabinose	-
68368	29016 ChEBI	arginine	-	from API 20E
68368	16947 ChEBI	citrate	+	from API 20E
68379	17634 ChEBI	D-glucose	-	from API Coryne
68379	16899 ChEBI	D-mannitol	+	from API Coryne
68379	16988 ChEBI	D-ribose	-	from API Coryne
68379	65327 ChEBI	D-xylose	-	from API Coryne
68379	4853 ChEBI	esculin	-	from API Coryne
18453	28757 ChEBI	fructose	-
68379	5291 ChEBI	gelatin	+	from API Coryne
18453	17234 ChEBI	glucose	-
68379	28087 ChEBI	glycogen	-	from API Coryne
68379	17716 ChEBI	lactose	-	from API Coryne
68368	25094 ChEBI	lysine	-	from API 20E
68379	17306 ChEBI	maltose	+	from API Coryne
18453	29864 ChEBI	mannitol	-
18453	17268 ChEBI	myo-inositol	-
68379	17632 ChEBI	nitrate	+	from API Coryne
123737	17632 ChEBI	nitrate	+
123737	16301 ChEBI	nitrite	-
68368	18257 ChEBI	ornithine	-	from API 20E
18453	16634 ChEBI	raffinose	-
18453	26546 ChEBI	rhamnose	-
18453	17992 ChEBI	sucrose	+
68379	17992 ChEBI	sucrose	+	from API Coryne
68368	27897 ChEBI	tryptophan	-	from API 20E
68379	16199 ChEBI	urea	+	from API Coryne
68368	16199 ChEBI	urea	+	from API 20E
18453	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
123737	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
68368	15688 ChEBI	acetoin	-	from API 20E
68368	35581 ChEBI	indole		from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68382	beta-galactosidase	-	3.2.1.23	from API zym
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	+	3.2.1.31	from API Coryne
123737	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68379	gelatinase	+		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
123737	oxidase	-
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
123737	urease	+	3.5.1.5
68379	urease	+	3.5.1.5	from API Coryne
68368	urease	+	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
18453	-	-	-	-	+	-	+	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
18453	+	+	+	-	+	+	+	-	-	+	+	not determinedn.d.	-	-	-	+	+	-	+	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18453	not determinedn.d.	+	+	+	-	+	+	+	-	-	+	+	-	-	-	+	+	-	+	-
123737	-	+	+	+	+	+	+	+	+	-	+	+	-	-	-	+	-	-	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type
8867	humus soil
67770	Humus soil
123737	Environment, Humus soil

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8867	1	Risk group (German classification) According to TRBA 466
18453	1
123737	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM3952694v1 assembly for Glutamicibacter uratoxydans JCM 11944	scaffold	GCA_039526945	43667	71.45
67770	ASM653992v1 assembly for Glutamicibacter uratoxydans NBRC 15515	contig	GCA_006539925	43667	66.59

Genome browser: GCA_039526945

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
8867	A.uratoxydans 16S rRNA gene	X83410	1484		43667

GC content

@ref	GC-content (mol%)
8867	61.2
67770	61.2

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Glutamicibacter uratoxydans NBRC 15515
NCBI: GCA_006539925

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.30	no
125439	motility	BacteriaNetⓘ	yes	75.40	no
125439	gram_stain	BacteriaNetⓘ	negative	96.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	60.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Glutamicibacter uratoxydans strain NBRC 15515
PATRIC: 43667.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	91.49	no
125438	anaerobic	anaerobicⓘ	no	96.85	yes
125438	spore-forming	spore-formingⓘ	no	73.09	no
125438	aerobic	aerobicⓘ	yes	87.45	yes
125438	thermophilic	thermophileⓘ	no	98.00	yes
125438	flagellated	motile2+ⓘ	no	93.10	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Virulent Phages Isolated from a Smear-Ripened Cheese Are Also Detected in Reservoirs of the Cheese Factory.	Paillet T, Lossouarn J, Figueroa C, Midoux C, Rue O, Petit MA, Dugat-Bony E.	Viruses	10.3390/v14081620	2022
	Evaluation of heavy metal removal and antibiofilm efficiency of biologically synthesized chitosan- silver Nano-bio composite by a soil actinobacterium Glutamicibacter uratoxydans VRAK 24.	Vishnupriya C, Mohamedrizwan K, Arya PR, Vijayakumar S, Kavitha R.	Int J Biol Macromol	10.1016/j.ijbiomac.2023.128032	2024
Enzymology	Fungal endo and exochitinase production, characterization, and application for Candida biofilm removal.	de Menezes CLA, Boscolo M, da Silva R, Gomes E, da Silva RR.	Braz J Microbiol	10.1007/s42770-024-01432-9	2024
	Glutamicibacter sp. ZY1 antagonizes pathogenic Vibrio parahaemolyticus via iron competition.	Shi Z, Li Y, Shi W, Mu Z, Han Q, Zhang W.	Appl Environ Microbiol	10.1128/aem.00009-25	2025
Metabolism	Structure of hexasaccharide 1-phosphate polymer from Arthrobacter uratoxydans VKM Ac-1979(T) cell wall.	Potekhina NV, Shashkov AS, Senchenkova SN, Dorofeeva LV, Evtushenko LI	Biochemistry (Mosc)	10.1134/S0006297912110089	2012

References

#8867	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20647
#18453	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#41914	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#123737	Collection of Institut Pasteur ; Curators of the CIP; CIP 102367
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Glutamicibacter uratoxydans (DSM 20647, ATCC 21749, U-23)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Murein

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_039526945

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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