Name: Arthrobacter pascens B89
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7531

Type strain:

Species: Arthrobacter pascens

Strain Designation: B89

Culture col. no.: DSM 20545, ATCC 13346, CCM 1653, NCIB 8910, CCUG 23843, JCM 11606, BCRC 12112, CDA 858, CIP 102362, HAMBI 1862, IAM 12343, IFO 12139, KCTC 3445, LMG 16255, NBRC 12139, NCIMB 8910, NRRL B-1814, VKM Ac-1116, VKM Ac-1128, IMET 10355

Strain history: CIP <- 1986, DSM <- CCM <- A.G. Lochhead: strain B89 Central Exp. Farm, Ottawa, Canada

NCBI tax ID(s): 1677 (species)

SI-ID 10202

LMG 16255, ATCC 13346, CCM 1653, DSM 20545, NCIB 8910, IFO 12139, CCUG 23843, CIP 102362, IMET 10355, NCIMB 8910, IAM12343, VKM Ac-1116, VKM Ac-1128, KCTC 3445, JCM 11606, NBRC 12139, NRRL B-1814, VKM B-665, HAMBI 1862, BCRC 12112, CCRC 12112

Section

Arthrobacter pascens B89 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from Soil.

Gram-positive
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Arthrobacter*
	Species	**Arthrobacter pascens**
	Full Scientific Name (LPSN)	Arthrobacter pascens Lochhead and Burton 1953 (Approved Lists 1980)

Information on morphological and physiological properties Morphology

[Ref.: #121615]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #121615]	Cell shape	rod-shaped

[Ref.: #121615]	Motility	no

[Ref.: #18447]	Colony color	Beige (1001)
[Ref.: #18447]	Incubation period	10-14 days
[Ref.: #18447]	Cultivation medium used	ISP 2

[Ref.: #18447]	Colony color	Light ivory (1015)
[Ref.: #18447]	Incubation period	10-14 days
[Ref.: #18447]	Cultivation medium used	ISP 3

[Ref.: #18447]	Colony color	Colorless
[Ref.: #18447]	Incubation period	10-14 days
[Ref.: #18447]	Cultivation medium used	ISP 4

[Ref.: #18447]	Colony color	Colorless
[Ref.: #18447]	Incubation period	10-14 days
[Ref.: #18447]	Cultivation medium used	ISP 5

[Ref.: #18447]	Colony color	Colorless
[Ref.: #18447]	Incubation period	10-14 days
[Ref.: #18447]	Cultivation medium used	ISP 6

[Ref.: #18447]	Colony color	Colorless
[Ref.: #18447]	Incubation period	10-14 days
[Ref.: #18447]	Cultivation medium used	ISP 7

[Ref.: #18447]	Multicellular complex forming ability	no
[Ref.: #18447]	Medium Name (multicellularity)	ISP 2

[Ref.: #18447]	Multicellular complex forming ability	no
[Ref.: #18447]	Medium Name (multicellularity)	ISP 3

[Ref.: #18447]	Multicellular complex forming ability	no
[Ref.: #18447]	Medium Name (multicellularity)	ISP 4

[Ref.: #18447]	Multicellular complex forming ability	no
[Ref.: #18447]	Medium Name (multicellularity)	ISP 5

[Ref.: #18447]	Multicellular complex forming ability	no
[Ref.: #18447]	Medium Name (multicellularity)	ISP 6

[Ref.: #18447]	Multicellular complex forming ability	no
[Ref.: #18447]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18447]

Culture medium

ISP 2

[Ref.: #18447]

Culture medium growth

[Ref.: #18447]

Culture medium composition

expand / minimize

[Ref.: #18447]

Culture medium

ISP 7

[Ref.: #18447]

Culture medium growth

[Ref.: #18447]

Culture medium composition

expand / minimize

[Ref.: #18447]

Culture medium

ISP 6

[Ref.: #18447]

Culture medium growth

[Ref.: #18447]

Culture medium composition

expand / minimize

[Ref.: #18447]

Culture medium

ISP 5

[Ref.: #18447]

Culture medium growth

[Ref.: #18447]

Culture medium composition

expand / minimize

[Ref.: #18447]

Culture medium

ISP 4

[Ref.: #18447]

Culture medium growth

[Ref.: #18447]

Culture medium composition

expand / minimize

[Ref.: #18447]

Culture medium

ISP 3

[Ref.: #18447]

Culture medium growth

[Ref.: #18447]

Culture medium composition

expand / minimize

[Ref.: #37391]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #37391]

Culture medium growth

[Ref.: #37391]

Culture medium composition

expand / minimize

[Ref.: #8774]

Culture medium

CORYNEBACTERIUM AGAR (DSMZ Medium 53)

[Ref.: #8774]

Culture medium growth

[Ref.: #8774]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8774]

Culture medium composition

expand / minimize

[Ref.: #121615]

Culture medium

CIP Medium 72

[Ref.: #121615]

Culture medium growth

[Ref.: #121615]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121615]

Culture medium

CIP Medium 3

[Ref.: #121615]

Culture medium growth

[Ref.: #121615]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8774]	growth	30 °C
[Ref.: #18447]	optimum	28 °C
[Ref.: #37391]	growth	30 °C
[Ref.: #47856]	growth	20-30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #8774]	Temperature range	mesophilic
[Ref.: #18447]	Temperature range	mesophilic
[Ref.: #37391]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #47856]	Oxygen tolerance	aerobe
[Ref.: #121615]	Oxygen tolerance	facultative anaerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.34

[Ref.: #8774]

Name of produced compound

terregens factor

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #18447]		NaCl		maximum	5	%

[Ref.: #8774]	Murein short key	A11.05
[Ref.: #8774]	Murein types	A3alpha L-Lys-L-Ala₂

[Ref.: #67770]

Observation

Production of "Terregens factor"

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #18447]	arabinose ChEBI	-
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18447]	cellulose ChEBI	-
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #18447]	fructose ChEBI	-
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18447]	glucose ChEBI	-
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #18447]	mannitol ChEBI	-
[Ref.: #18447]	myo-inositol ChEBI	-
[Ref.: #121615]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #121615]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #18447]	raffinose ChEBI	+
[Ref.: #18447]	sucrose ChEBI	-
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E, API Coryne
[Ref.: #18447]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #121615]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API Coryne, API 20E
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API Coryne
[Ref.: #68379]	beta-glucuronidase	+	3.2.1.31	* from API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121615]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #121615]	oxidase	-
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #121615]	urease	-	3.5.1.5
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne, API 20E
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #18447]	37	+	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18447]	124	-	+	+	-	+	+	+	-	-	-	+/-	not determinedn.d.	-	+	-	+	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18447]	159	not determinedn.d.	-	+	+	-	+	+	+	-	-	-	not determinedn.d.	-	+	-	+	-	-	-	-
[Ref.: #121615]	7037	-	+	+	+	+	+	+	+	+	-	+	+	+	+	-	+	-	-	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Soil

[Ref.: #121615]	Sample type/isolated from	Environment, Soil
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence X80740 (>99% sequence identity) for Arthrobacter from Microbeatlas

Aquatic Samples	227
Soil Samples	1582
Animal Samples	159
Plant Samples	3347
Total Samples	5315

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18447]	Biosafety level	1
[Ref.: #8774]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121615]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8774]	A.pascens 16S rDNA	X80740	1474	ENA	1677 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Arthrobacter pascens DSM 20545	GCA_017052465	contig	GenBank	1677 tax ID	*
[Ref.: #66792]	Arthrobacter pascens strain DSM 20545	1677.4	wgs	PATRIC	1677 tax ID	*

[Ref.: #8774]	GC-content	63.7 mol%
[Ref.: #67770]	GC-content	63.7 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	64.9 mol%	Buoyant density centrifugation (BD)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Arthrobacter pascens strain DSM 20545 PATRIC: 1677.4
[Ref.: #69481]	spore-forming	no	80	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Arthrobacter pascens strain DSM 20545 PATRIC: 1677.4
[Ref.: #69480]	motile	no	89.357	no
[Ref.: #69480]	gram-positive	yes	92.865	no
[Ref.: #69480]	anaerobic	no	99.538	yes
[Ref.: #69480]	aerobic	yes	96.704	yes
[Ref.: #69480]	halophile	no	81.474	no
[Ref.: #69480]	spore-forming	no	86.339	no
[Ref.: #69480]	glucose-util	yes	80.634	yes
[Ref.: #69480]	flagellated	no	96.469	no
[Ref.: #69480]	thermophile	no	98.763	yes
[Ref.: #69480]	glucose-ferment	no	86.358	no

Availability in culture collections External links

[Ref.: #8774]

Culture collection no.

DSM 20545, ATCC 13346, CCM 1653, NCIB 8910, CCUG 23843, JCM 11606, BCRC 12112, CDA 858, CIP 102362, HAMBI 1862, IAM 12343, IFO 12139, KCTC 3445, LMG 16255, NBRC 12139, NCIMB 8910, NRRL B-1814, VKM Ac-1116, VKM Ac-1128, IMET 10355

[Ref.: #76976]

SI-ID 10202

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Arthrobacter pokkalii sp nov, a Novel Plant Associated Actinobacterium with Plant Beneficial Properties, Isolated from Saline Tolerant Pokkali Rice, Kerala, India.	Krishnan R, Menon RR, Tanaka N, Busse HJ, Krishnamurthi S, Rameshkumar N	PLoS One	10.1371/journal.pone.0150322	2016	*
Phylogeny	Citricoccus muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting.	Altenburger P, Kampfer P, Schumann P, Steiner R, Lubitz W, Busse HJ	Int J Syst Evol Microbiol	10.1099/00207713-52-6-2095	2002	*

References

#8774

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20545

#18447

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#37391 ; Curators of the CIP;
#47856 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 23843

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76976

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID10202.1 )

#121615 Collection of Institut Pasteur ; Curators of the CIP; CIP 102362
* These data were automatically processed and therefore are not curated

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Arthrobacter pascens strain DSM 20545

Arthrobacter pascens strain DSM 20545

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