Agromyces mediolanus (DSM 20152, ATCC 14004, JCM 3346)

Name: Agromyces mediolanus (DSM 20152, ATCC 14004, JCM 3346)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7265

Type strain

Culture col. no. DSM 20152 ATCC 14004 JCM 3346 NCIB 7206 VKM Ac-1388 5 more

NCBI tax ID(s) 41986

Special Collections DSMZ JCM KCTC CIP Wink compendium

History <- ATCC <- NCIB <- NCTC <- F.M. Berger <- Metropolitan Police Lab. <- A. Ercoli and L. Molina (Corynebacterium mediolanum) N. S. Agre <-- D. Jones <-- NCIB 7206 <-- F. M. Berger <-- Metropolitan Police Lab. <-- A. Ercoli and L. Molina. CIP <- 1996, JCM <- N.S. Agre <- D. Jones <- NCIB <- F.M. Berger <- Metropolitan Police Lab. <- A. Ercoli and L. Molina

Links

Agromyces mediolanus DSM 20152 is an obligate aerobe, Gram-positive, rod-shaped bacterium of the family Microbacteriaceae.

Gram-positive rod-shaped obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 8227

LMG 17243,ATCC 14004,DSM 20152,IFO 15704,JCM 3346,KCTC 3136,NCIMB 7206,NCTC 7206,NRRL B-16529,NCIB 7206,CIP 104860,VKM Ac-1388,NBRC 15704

@ref 20215

Last LPSN update 2026-02-09 11:19:25

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Microbacteriaceae

Genus Agromyces

Species Agromyces mediolanus

Full scientific name Agromyces mediolanus (ex Mamoli 1939) Suzuki et al. 1996

BacDive ID	Other strains from **Agromyces mediolanus** (8)	Type strain
7264	A. mediolanus DSM 40, ATCC 13930, JCM 1376, IAM 15149, ...
103272	A. mediolanus ST033323(HKI),
146260	A. mediolanus CCUG 30720
148068	A. mediolanus CCUG 34759
156798	A. mediolanus CCUG 65378
166041	A. mediolanus JCM 9631, LMG 18118
166042	A. mediolanus JCM 9632
166043	A. mediolanus JCM 9633

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
120869	positive	rod-shaped
125439	positive		99.4
125438	positive		90.329

Colony morphology

@ref	Colony color	Incubation period	Medium used
18426	Beige (1001)	10-14 days	ISP 2
18426	Beige (1001)	10-14 days	ISP 3
18426	Beige (1001)	10-14 days	ISP 4
18426	Beige (1001)	10-14 days	ISP 5
18426	Beige (1001)	10-14 days	ISP 6
18426	Beige (1001)	10-14 days	ISP 7
120869

Multicellular morphology

@ref	Forms multicellular complex	Medium name
18426		ISP 2
18426		ISP 3
18426		ISP 4
18426		ISP 5
18426		ISP 6
18426		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8576	CORYNEBACTERIUM AGAR (DSMZ Medium 53)	Medium recipe at MediaDive	Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water
18426	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18426	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18426	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18426	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18426	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18426	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
37050	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
120869	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
8576	positive	growth	30
18426	positive	optimum	28
37050	positive	growth	30
67770	positive	growth	30
120869	positive	growth	25-41
120869	negative	growth	10
120869	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
120869	obligate aerobe
125439	obligate aerobe	98.9

Compound production

8576

Compoundsteroids

Halophily

@ref	Salt	Growth	Tested relation	Concentration
120869	NaCl	positive	growth	0-6 %
120869	NaCl		growth	8 %
120869	NaCl		growth	10 %

Murein

@ref	Murein short key	Type
8576	B07	B2gamma {Gly} [L-Dab] D-Glu-D-Dab

Observation

67770

Observationquinones: MK-12, MK-11

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	+	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
120869	16947 ChEBI	citrate	-	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
120869	4853 ChEBI	esculin	+	hydrolysis
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	+	builds acid from	from API 50CH acid
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
120869	606565 ChEBI	hippurate	-	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	+	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	+	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
120869	17632 ChEBI	nitrate	-	reduction
120869	17632 ChEBI	nitrate	-	respiration
120869	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
120869	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120869	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
120869	15688 ChEBI	acetoin	-
120869	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120869	alcohol dehydrogenase	-	1.1.1.1
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	+	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
120869	amylase	-
68382	beta-galactosidase	+	3.2.1.23	from API zym
120869	beta-galactosidase	+	3.2.1.23
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
120869	caseinase	-	3.4.21.50
120869	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
120869	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
120869	gamma-glutamyltransferase	-	2.3.2.2
120869	gelatinase	-
68379	gelatinase	-		from API Coryne
120869	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
120869	lipase	-
120869	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120869	ornithine decarboxylase	-	4.1.1.17
120869	oxidase	-
120869	phenylalanine ammonia-lyase	-	4.3.1.24
120869	protease	-
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
120869	tryptophan deaminase	-
120869	tween esterase	-
120869	urease	-	3.5.1.5
68379	urease	+	3.5.1.5	from API Coryne
68382	valine arylamidase	+		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
18426	-	+	+	+	-	+	+	-	+	+	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18426	not determinedn.d.	+	+	+	+	+	+	+	-	-	+	+	-	+	-	+	+	+	-	+
120869	-	-	+	+	-	+	+	+	+	-	+	+	-	+	-	+	+	+	-	+

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
120869	not determinedn.d.	+	-	-	-	+	-	-	-	-	+	+	+	+	-	+	-	-	-	-	-	-	-	+	+	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
120869	not determinedn.d.	+	-	+	+	+	-	-	-	-	-	+	-	-	-	-	+	-	-	+	+	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Taxonmaps

69479

Global distribution of 16S sequence X77449 (>99% sequence identity) for Agromyces mediolanus from Microbeatlas

Aquatic Samples	780
Soil Samples	3220
Animal Samples	1854
Plant Samples	557
Total Samples	6411

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8576	1	Risk group (German classification) According to TRBA 466
18426	1	German classification According to TRBA 466
120869	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM4264680v1 assembly for Agromyces mediolanus JCM 3346	contig	GCA_042646805			41986	55.73
66792	ASM1464857v1 assembly for Agromyces mediolanus JCM 3346	scaffold	GCA_014648575	41986.5	3002691605	41986	52.66
124043	ASM2792182v1 assembly for Agromyces mediolanus	scaffold	GCA_027921825	41986.10		41986

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Agromyces mediolanus strain JCM3346 16S ribosomal RNA gene, partial sequence	HM641754	1372	41986
67770	Agromyces mediolanus strain VKM Ac-1388 16S ribosomal RNA gene, partial sequence	JX186590	1479	41986
67770	C.medislanum (DSM 20152) 16S rRNA gene	X77449	1517	41986
8576	Agromyces mediolanus strain DSM 20152 16S ribosomal RNA gene, complete sequence	NR_044857	1517	41986
124043	Agromyces mediolanus strain JCM 3346 16S ribosomal RNA gene, partial sequence.	MT760454	1355	41986

GC content

@ref	GC-content (mol%)	Method
8576	74.7
67770	74.4	thermal denaturation, midpoint method (Tm)
67770	72.3	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Agromyces mediolanus JCM 3346
NCBI: GCA_014648575

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	55.70	no
125439	motility	BacteriaNetⓘ	no	66.10	no
125439	gram_stain	BacteriaNetⓘ	positive	99.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Agromyces mediolanus JCM 3346
NCBI: GCA_014648575.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.33	no
125438	anaerobic	anaerobicⓘ	no	97.72	no
125438	aerobic	aerobicⓘ	yes	88.73	yes
125438	spore-forming	spore-formingⓘ	no	74.25	no
125438	thermophilic	thermophileⓘ	no	97.47	yes
125438	flagellated	motile2+ⓘ	no	79.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Isolation and characterization of endophytic bacteria isolated from the leaves of the common bean (Phaseolus vulgaris).	de Oliveira Costa LE, de Queiroz MV, Borges AC, de Moraes CA, de Araujo EF.	Braz J Microbiol	10.1590/s1517-838220120004000041	2012
	Reevaluation and reduction of a PCR bias caused by reannealing of templates.	Kurata S, Kanagawa T, Magariyama Y, Takatsu K, Yamada K, Yokomaku T, Kamagata Y.	Appl Environ Microbiol	10.1128/aem.70.12.7545-7549.2004	2004
Enzymology	First Report of Human Infection by Agromyces mediolanus, a Gram-Positive Organism Found in Soil.	Sridhar S, Wang AY, Chan JF, Yip CC, Lau SK, Woo PC, Yuen KY.	J Clin Microbiol	10.1128/jcm.01508-15	2015
	A simplified synthetic community from the healthy watermelon rhizosphere effectively mitigates Fusarium wilt.	Yu H, Wang Z, Tian R, Xu W.	Pest Manag Sci	10.1002/ps.70193	2025
	A rare case of long-term dialysis catheter-associated Agromyces mediolanus bacteremia: A case report and literature review.	Haq K, Pan S, Pacheco E, Ghaly M.	Clin Nephrol Case Stud	10.5414/cncs110980	2023
	Species-specific Microorganisms in Acid-tolerant Chironomus Larvae Reared in a Neutral pH Range under Laboratory Conditions: Single Dataset Analysis.	Fujii S, Kawai K, Sambongi Y, Wakai S.	Microbes Environ	10.1264/jsme2.me23029	2023
Metabolism	Enantioselective Hydrolysis of Styrene Oxide and Benzyl Glycidyl Ether by a Variant of Epoxide Hydrolase from Agromyces mediolanus.	Jin H, Li Y, Zhang Q, Lin S, Yang Z, Ding G.	Mar Drugs	10.3390/md17060367	2019
	Purification, gene cloning, and characterization of a novel halohydrin dehalogenase from Agromyces mediolanus ZJB120203.	Xue F, Liu ZQ, Wan NW, Zheng YG.	Appl Biochem Biotechnol	10.1007/s12010-014-1111-z	2014
Metabolism	Biosynthetic Potential of a Novel Antarctic Actinobacterium Marisediminicola antarctica ZS314^T Revealed by Genomic Data Mining and Pigment Characterization.	Liao L, Su S, Zhao B, Fan C, Zhang J, Li H, Chen B.	Mar Drugs	10.3390/md17070388	2019
Metabolism	Response of Rhodococcus cerastii IEGM 1278 to toxic effects of ibuprofen.	Ivshina IB, Tyumina EA, Bazhutin GA, Vikhareva EV.	PLoS One	10.1371/journal.pone.0260032	2021
Metabolism	The metabolism of neonicotinoid insecticide thiamethoxam by soil enrichment cultures, and the bacterial diversity and plant growth-promoting properties of the cultured isolates.	Zhou GC, Wang Y, Ma Y, Zhai S, Zhou LY, Dai YJ, Yuan S.	J Environ Sci Health B	10.1080/03601234.2014.894761	2014
	Application of cold-adapted microbial agents in soil contaminate remediation: biodegradation mechanisms, case studies, and safety assessments.	Li J, Wen Y, Fang Z, Yang W, Song X.	RSC Adv	10.1039/d4ra01510j	2024
Phylogeny	Microbiological Characteristics of Some Stations of Moscow Subway.	Pochtovyi AA, Vasina DV, Verdiev BI, Shchetinin AM, Yuzhakov AG, Ovchinnikov RS, Tkachuk AP, Gushchin VA, Gintsburg AL.	Biology (Basel)	10.3390/biology11020170	2022
	Comparison of the carotenoid profiles of commonly consumed smear-ripened cheeses.	Yeluri Jonnala BR, McSweeney PLH, Cotter PD, Zhong S, Sheehan JJ, Kopec RE.	Lebensm Wiss Technol	10.1016/j.lwt.2020.110241	2021
	Genes from a Dietzia sp. for synthesis of C40 and C50 beta-cyclic carotenoids.	Tao L, Yao H, Cheng Q.	Gene	10.1016/j.gene.2006.08.006	2007
	Evaluating Ylehd, a recombinant epoxide hydrolase from Yarrowia lipolytica as a potential biocatalyst for the resolution of benzyl glycidyl ether.	Bendigiri C, Harini K, Yenkar S, Zinjarde S, Sowdhamini R, RaviKumar A.	RSC Adv	10.1039/c8ra00628h	2018
Phylogeny	Agromyces endophyticus sp. nov., isolated from endosphere of garlic.	Kim S, Heo J, Choi H, Choi Y, Weon HY, Kwon SW, Naito H, Yamada T, Hamada M, Kim Y.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006948	2025
Phylogeny	Agromyces protaetiae sp. nov., isolated from gut of larva of Protaetia brevitarsis seulensis.	Heo J, Hamada M, Tamura T, Saito S, Lee SD, Kim JS, Hong SB, Kwon SW, Kim SJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.003908	2020
Phylogeny	Agromyces ulmi sp. nov., a xylanolytic bacterium isolated from Ulmus nigra in Spain.	Rivas R, Trujillo ME, Mateos PF, Martinez-Molina E, Velazquez E	Int J Syst Evol Microbiol	10.1099/ijs.0.63058-0	2004
Phylogeny	Agromyces mediolanus sp. nov., nom. rev., comb. nov., a species for "Corynebacterium mediolanum" Mamoli 1939 and for some aniline-assimilating bacteria which contain 2,4-diaminobutyric acid in the cell wall peptidoglycan.	Suzuki K, Sasaki J, Uramoto M, Nakase T, Komagata K	Int J Syst Bacteriol	10.1099/00207713-46-1-88	1996
Phylogeny	Agromyces soli sp. nov., isolated from farm soil.	Lee M, Ten LN, Woo SG, Park J	Int J Syst Evol Microbiol	10.1099/ijs.0.021568-0	2010

References

#8576	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20152
#18426	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#37050	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120869	Collection of Institut Pasteur ; Curators of the CIP; CIP 104860
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Agromyces mediolanus (DSM 20152, ATCC 14004, JCM 3346)

Name and taxonomic classification

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