Name: Methanosarcina thermophila TM-1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 7121

Type strain:

Species: Methanosarcina thermophila

Strain Designation: TM-1

Culture col. no.: DSM 1825, ATCC 43570, OCM 12, VKM B-1830

Strain history: <- P. Scherer <- S.H. Zinder, TM-1

NCBI tax ID(s): 523844 (strain), 2210 (species)

SI-ID 41920

ATCC 43570, DSM 1825, VKM B-1830, BCRC 16174, CCRC 16174

Special Collections

DSMZ

Other strains from Methanosarcina thermophila

Section

Methanosarcina thermophila TM-1 is an anaerobe, Gram-negative archaeon that produces methane and was isolated from thermophilic digester sludge.

methane production
Gram-negative
anaerobe
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	"Methanomicrobia"
	Order	*Methanosarcinales*
	Family	*Methanosarcinaceae*
	Genus	*Methanosarcina*
	Species	**Methanosarcina thermophila**
	Full Scientific Name (LPSN)	Methanosarcina thermophila Zinder et al. 1985

Information on culture and growth conditions Culture and growth conditions

[Ref.: #668]

Culture medium

METHANOSARCINA MEDIUM (DSMZ Medium 120)

[Ref.: #668]

Culture medium growth

[Ref.: #668]

Culture medium link

Medium recipe at MediaDive

[Ref.: #668]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #668]			growth	50 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #668]

Oxygen tolerance

anaerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %90.123

[Ref.: #668]

Name of produced compound

methane

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #668]	Sample type/isolated from	thermophilic digester sludge
[Ref.: #668]	Geographic location (country and/or sea, region)	California, El Segundo
[Ref.: #668]	Country	USA
[Ref.: #668]	Country ISO 3 Code	USA
[Ref.: #668]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	-
#Environmental	#Terrestrial	#Mud (Sludge)
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #668]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #668]	Methanosarcina thermophila TM-1 strain DSM 1825 16S ribosomal RNA gene, partial sequence	M59140	1427	GenBank ENA	523844 tax ID
[Ref.: #20218]	Methanosarcina thermophila TM-1 culture DSM:1825 16S ribosomal RNA gene, partial sequence	JQ346758	1268	GenBank ENA	523844 tax ID	*
[Ref.: #124043]	Methanosarcina thermophila gene for 16S ribosomal RNA, partial sequence, strain: DSM 1825.	AB973357	1409	GenBank ENA	523844 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanosarcina thermophila TM-1	GCA_000969885	complete	GenBank ENA	523844 tax ID	*
[Ref.: #66792]	Methanosarcina thermophila TM-1	2636415612	complete	JGI IMG/M	523844 tax ID	*
[Ref.: #66792]	Methanosarcina thermophila TM-1	523844.20	complete	PATRIC	523844 tax ID	*

[Ref.: #668]

GC-content

42.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methanosarcina thermophila TM-1 TM-1 NCBI: GCA_000969885.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	64.78	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	83.101	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	88.793	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	90.123	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	54.636	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	82.458	no

Availability in culture collections External links

[Ref.: #668]

Culture collection no.

DSM 1825, ATCC 43570, OCM 12, VKM B-1830

[Ref.: #76576]

SI-ID 41920

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Non-autotrophic methanogens dominate in anaerobic digesters.	Kouzuma A, Tsutsumi M, Ishii S, Ueno Y, Abe T, Watanabe K	Sci Rep	10.1038/s41598-017-01752-x	2017	*
Phylogeny	Methanosarcina flavescens sp. nov., a methanogenic archaeon isolated from a full-scale anaerobic digester.	Kern T, Fischer MA, Deppenmeier U, Schmitz RA, Rother M	Int J Syst Evol Microbiol	10.1099/ijsem.0.000894	2016	*
Enzymology	Growth kinetics of thermophilic Methanosarcina spp. isolated from full-scale biogas plants treating animal manures.	Mladenovska Z, Ahring BK	FEMS Microbiol Ecol	10.1111/j.1574-6941.2000.tb00687.x	2000	*
Enzymology	The genes coding for the hsp70 (dnaK) molecular chaperone machine occur in the moderate thermophilic archaeon Methanosarcina thermophila TM-1.	Hofman-Bang J, Lange M, Conway de Macario E, Macario AJ, Ahring BK	Gene	10.1016/s0378-1119(99)00343-1	1999	*
Metabolism	Methanopterin biosynthesis: methylation of the biosynthetic intermediates.	White RH	Biochim Biophys Acta	10.1016/s0304-4165(97)00148-7	1998	*
Metabolism	Gene cloning and expression and characterization of a toxin-sensitive protein phosphatase from the methanogenic archaeon Methanosarcina thermophila TM-1.	Solow B, Young JC, Kennelly PJ	J Bacteriol	10.1128/jb.179.16.5072-5075.1997	1997	*
Metabolism	The methanogenic archaeon Methanosarcina thermophila TM-1 possesses a high-affinity glycine betaine transporter involved in osmotic adaptation.	Proctor LM, Lai R, Gunsalus RP	Appl Environ Microbiol	10.1128/aem.63.6.2252-2257.1997	1997	*
Metabolism	Protein-tyrosine phosphorylation in the Archaea.	Smith SC, Kennelly PJ, Potts M	J Bacteriol	10.1128/jb.179.7.2418-2420.1997	1997	*
Phylogeny	Bacterial artificial chromosome (BAC) library as a tool for physical mapping of the archaeon Methanosarcina thermophila TM-1.	Diaz-Perez SV, Alatriste-Mondragon F, Hernandez R, Birren B, Gunsalus RP	Microb Comp Genomics	10.1089/omi.1.1997.2.275	1997	*
Enzymology	Cloning, sequence analysis, and hyperexpression of the genes encoding phosphotransacetylase and acetate kinase from Methanosarcina thermophila.	Latimer MT, Ferry JG	J Bacteriol	10.1128/jb.175.21.6822-6829.1993	1993	*
Phylogeny	Inhibition of an archaeal protein phosphatase activity by okadaic acid, microcystin-LR, or calyculin A.	Oxenrider KA, Rasche ME, Thorsteinsson MV, Kennelly PJ	FEBS Lett	10.1016/0014-5793(93)80355-x	1993	*
Metabolism	Effect of medium composition and sludge removal on the production, composition, and architecture of thermophilic (55 degrees C) acetate-utilizing granules from an upflow anaerobic sludge blanket reactor.	Ahring BK, Schmidt JE, Winther-Nielsen M, Macario AJ, Conway de Macario E	Appl Environ Microbiol	10.1128/aem.59.8.2538-2545.1993	1993	*
Metabolism	Carbon Monoxide, Hydrogen, and Formate Metabolism during Methanogenesis from Acetate by Thermophilic Cultures of Methanosarcina and Methanothrix Strains.	Zinder SH, Anguish T	Appl Environ Microbiol	10.1128/aem.58.10.3323-3329.1992	1992	*
Metabolism	Effect of magnesium on methanogenic subpopulations in a thermophilic acetate-degrading granular consortium.	Schmidt JE, Macario AJ, Ahring BK, Conway de Macario E	Appl Environ Microbiol	10.1128/aem.58.3.862-868.1992	1992	*
Enzymology	Purification and properties of methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila.	Jablonski PE, Ferry JG	J Bacteriol	10.1128/jb.173.8.2481-2487.1991	1991	*
Enzymology	Protein content and enzyme activities in methanol- and acetate-grown Methanosarcina thermophila.	Jablonski PE, DiMarco AA, Bobik TA, Cabell MC, Ferry JG	J Bacteriol	10.1128/jb.172.3.1271-1275.1990	1990	*
Cultivation	Adaptation for growth at various saline concentrations by the archaebacterium Methanosarcina thermophila.	Sowers KR, Gunsalus RP	J Bacteriol	10.1128/jb.170.2.998-1002.1988	1988	*
	Establishment and Characterization of an Anaerobic Thermophilic (55(deg)C) Enrichment Culture Degrading Long-Chain Fatty Acids.	Angelidaki I, Ahring BK	Appl Environ Microbiol	10.1128/aem.61.6.2442-2445.1995	1995	*

References

#668	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1825

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76576

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID41920.1 )

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

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Methanosarcina thermophila TM-1 TM-1

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