Name: Methanosarcina thermophila TM-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7121

Type strain:

Species: Methanosarcina thermophila

Strain Designation: TM-1

Culture col. no.: DSM 1825, ATCC 43570, OCM 12, VKM B-1830

Strain history: <- P. Scherer <- S.H. Zinder, TM-1

NCBI tax ID(s): 523844 (strain), 2210 (species)

SI-ID 41920

ATCC 43570, DSM 1825, VKM B-1830, BCRC 16174, CCRC 16174

Other strains from Methanosarcina thermophila

M. thermophila MST-A1, DSM 2905, OCM 96
M. thermophila CHTI-55, DSM 2906, ATCC 43170, ...
M. thermophila MP, DSM 2980, OCM 91
M. thermophila DSM 7081, MCM B705

Section

Methanosarcina thermophila TM-1 is an anaerobe, thermophilic archaeon that produces methane and was isolated from thermophilic digester sludge.

methane production
anaerobe
thermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	"Methanomicrobia"
	Order	*Methanosarcinales*
	Family	*Methanosarcinaceae*
	Genus	*Methanosarcina*
	Species	**Methanosarcina thermophila**
	Full Scientific Name (LPSN)	Methanosarcina thermophila Zinder et al. 1985

Information on culture and growth conditions Culture and growth conditions

[Ref.: #668]

Culture medium

METHANOSARCINA MEDIUM (DSMZ Medium 120)

[Ref.: #668]

Culture medium growth

[Ref.: #668]

Culture medium link

Medium recipe at MediaDive

[Ref.: #668]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #668]			growth	50 °C

[Ref.: #668]

Temperature range

thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #668]

Oxygen tolerance

anaerobe

[Ref.: #668]

Name of produced compound

methane

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #668]	Sample type/isolated from	thermophilic digester sludge
[Ref.: #668]	Geographic location (country and/or sea, region)	California, El Segundo
[Ref.: #668]	Country	USA
[Ref.: #668]	Country ISO 3 Code	USA
[Ref.: #668]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	-
#Environmental	#Terrestrial	#Mud (Sludge)
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #668]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #668]	Methanosarcina thermophila TM-1 strain DSM 1825 16S ribosomal RNA gene, partial sequence	M59140	1427	ENA	523844 tax ID
[Ref.: #20218]	Enterococcus faecalis clone OCM012 16S ribosomal RNA gene, partial sequence	GU417214	455	ENA	1351 tax ID	*
[Ref.: #20218]	Methanosarcina thermophila TM-1 16S ribosomal RNA gene, partial sequence	JQ346758	1268	ENA	523844 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanosarcina thermophila TM-1	GCA_000969885	complete	GenBank	523844 tax ID	*
[Ref.: #66792]	Methanosarcina thermophila TM-1	2636415612	complete	JGI IMG/M	523844 tax ID	*
[Ref.: #66792]	Methanosarcina thermophila TM-1	523844.20	complete	PATRIC	523844 tax ID	*

[Ref.: #668]

GC-content

42.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methanosarcina thermophila TM-1 TM-1 NCBI: GCA_000969885.1
[Ref.: #69480]	motile	no	88.757	no
[Ref.: #69480]	flagellated	no	97.568	no
[Ref.: #69480]	gram-positive	no	95.533	no
[Ref.: #69480]	anaerobic	yes	96.598	yes
[Ref.: #69480]	aerobic	no	93.554	yes
[Ref.: #69480]	halophile	no	87.958	no
[Ref.: #69480]	spore-forming	no	95.183	no
[Ref.: #69480]	thermophile	no	70.186	no
[Ref.: #69480]	glucose-util	yes	68.483	no
[Ref.: #69480]	glucose-ferment	no	71.66	no

Availability in culture collections External links

[Ref.: #668]

Culture collection no.

DSM 1825, ATCC 43570, OCM 12, VKM B-1830

[Ref.: #76576]

SI-ID 41920

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Non-autotrophic methanogens dominate in anaerobic digesters.	Kouzuma A, Tsutsumi M, Ishii S, Ueno Y, Abe T, Watanabe K	Sci Rep	10.1038/s41598-017-01752-x	2017	*
Phylogeny	Methanosarcina flavescens sp. nov., a methanogenic archaeon isolated from a full-scale anaerobic digester.	Kern T, Fischer MA, Deppenmeier U, Schmitz RA, Rother M	Int J Syst Evol Microbiol	10.1099/ijsem.0.000894	2016	*
Enzymology	Growth kinetics of thermophilic Methanosarcina spp. isolated from full-scale biogas plants treating animal manures.	Mladenovska Z, Ahring BK	FEMS Microbiol Ecol	10.1111/j.1574-6941.2000.tb00687.x	2000	*
Enzymology	The genes coding for the hsp70 (dnaK) molecular chaperone machine occur in the moderate thermophilic archaeon Methanosarcina thermophila TM-1.	Hofman-Bang J, Lange M, Conway de Macario E, Macario AJ, Ahring BK	Gene	10.1016/s0378-1119(99)00343-1	1999	*
Metabolism	Methanopterin biosynthesis: methylation of the biosynthetic intermediates.	White RH	Biochim Biophys Acta	10.1016/s0304-4165(97)00148-7	1998	*
Metabolism	Gene cloning and expression and characterization of a toxin-sensitive protein phosphatase from the methanogenic archaeon Methanosarcina thermophila TM-1.	Solow B, Young JC, Kennelly PJ	J Bacteriol	10.1128/jb.179.16.5072-5075.1997	1997	*
Metabolism	The methanogenic archaeon Methanosarcina thermophila TM-1 possesses a high-affinity glycine betaine transporter involved in osmotic adaptation.	Proctor LM, Lai R, Gunsalus RP	Appl Environ Microbiol	10.1128/aem.63.6.2252-2257.1997	1997	*
Metabolism	Protein-tyrosine phosphorylation in the Archaea.	Smith SC, Kennelly PJ, Potts M	J Bacteriol	10.1128/jb.179.7.2418-2420.1997	1997	*
Phylogeny	Bacterial artificial chromosome (BAC) library as a tool for physical mapping of the archaeon Methanosarcina thermophila TM-1.	Diaz-Perez SV, Alatriste-Mondragon F, Hernandez R, Birren B, Gunsalus RP	Microb Comp Genomics	10.1089/omi.1.1997.2.275	1997	*
Enzymology	Cloning, sequence analysis, and hyperexpression of the genes encoding phosphotransacetylase and acetate kinase from Methanosarcina thermophila.	Latimer MT, Ferry JG	J Bacteriol	10.1128/jb.175.21.6822-6829.1993	1993	*
Phylogeny	Inhibition of an archaeal protein phosphatase activity by okadaic acid, microcystin-LR, or calyculin A.	Oxenrider KA, Rasche ME, Thorsteinsson MV, Kennelly PJ	FEBS Lett	10.1016/0014-5793(93)80355-x	1993	*
Metabolism	Effect of medium composition and sludge removal on the production, composition, and architecture of thermophilic (55 degrees C) acetate-utilizing granules from an upflow anaerobic sludge blanket reactor.	Ahring BK, Schmidt JE, Winther-Nielsen M, Macario AJ, Conway de Macario E	Appl Environ Microbiol	10.1128/aem.59.8.2538-2545.1993	1993	*
Metabolism	Carbon Monoxide, Hydrogen, and Formate Metabolism during Methanogenesis from Acetate by Thermophilic Cultures of Methanosarcina and Methanothrix Strains.	Zinder SH, Anguish T	Appl Environ Microbiol	10.1128/aem.58.10.3323-3329.1992	1992	*
Metabolism	Effect of magnesium on methanogenic subpopulations in a thermophilic acetate-degrading granular consortium.	Schmidt JE, Macario AJ, Ahring BK, Conway de Macario E	Appl Environ Microbiol	10.1128/aem.58.3.862-868.1992	1992	*
Enzymology	Purification and properties of methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila.	Jablonski PE, Ferry JG	J Bacteriol	10.1128/jb.173.8.2481-2487.1991	1991	*
Enzymology	Protein content and enzyme activities in methanol- and acetate-grown Methanosarcina thermophila.	Jablonski PE, DiMarco AA, Bobik TA, Cabell MC, Ferry JG	J Bacteriol	10.1128/jb.172.3.1271-1275.1990	1990	*
Cultivation	Adaptation for growth at various saline concentrations by the archaebacterium Methanosarcina thermophila.	Sowers KR, Gunsalus RP	J Bacteriol	10.1128/jb.170.2.998-1002.1988	1988	*
	Establishment and Characterization of an Anaerobic Thermophilic (55(deg)C) Enrichment Culture Degrading Long-Chain Fatty Acids.	Angelidaki I, Ahring BK	Appl Environ Microbiol	10.1128/aem.61.6.2442-2445.1995	1995	*

References

#668	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1825

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76576

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID41920.1 )

* These data were automatically processed and therefore are not curated

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Methanosarcina thermophila TM-1 TM-1

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