Methanocaldococcus villosus (DSM 22612, JCM 16315, KIN24-T80)

Name: Methanocaldococcus villosus (DSM 22612, JCM 16315, KIN24-T80)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6986

Type strain

Strain Designation KIN24-T80

Culture col. no. DSM 22612 JCM 16315 KIN24-T80

NCBI tax ID(s) 667126

Special Collections DSMZ JCM

Links

Methanocaldococcus villosus KIN24-T80 is an anaerobe, hyperthermophilic prokaryote that was isolated from hot marine sediment.

anaerobe hyperthermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 397262

JCM 16315,DSM 22612

@ref 20215

Last LPSN update 2025-12-16 09:48:28

Domain Methanobacteriati

Phylum Methanobacteriota

Class Methanococci

Order Methanococcales

Family Methanocaldococcaceae

Genus Methanocaldococcus

Species Methanocaldococcus villosus

Full scientific name Methanocaldococcus villosus Bellack et al. 2011

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
16431	METHANOCALDOCOCCUS VILLOSUS MEDIUM (DSMZ Medium 282a)		Medium recipe at MediaDive	Name: METHANOCALDOCOCCUS VILLOSUS MEDIUM (DSMZ Medium 282a) Composition: MgCl2 x 6 H2O 4.05138 g/l NaHCO3 0.988142 g/l Na2S x 9 H2O 0.494071 g/l L-Cysteine HCl x H2O 0.494071 g/l KCl 0.326087 g/l NH4Cl 0.247036 g/l K2HPO4 0.13834 g/l MgSO4 x 7 H2O 0.0296443 g/l Nitrilotriacetic acid 0.0148221 g/l NaCl 0.00988142 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00988142 g/l MnSO4 x H2O 0.00494071 g/l ZnSO4 x 7 H2O 0.00177866 g/l CoSO4 x 7 H2O 0.00177866 g/l CaCl2 x 2 H2O 0.000988142 g/l FeSO4 x 7 H2O 0.000988142 g/l Sodium resazurin 0.000494071 g/l NiCl2 x 6 H2O 0.000296443 g/l AlK(SO4)2 x 12 H2O 0.000197628 g/l Na2MoO4 x 2 H2O 9.88142e-05 g/l CuSO4 x 5 H2O 9.88142e-05 g/l H3BO3 9.88142e-05 g/l Pyridoxine hydrochloride 9.88142e-05 g/l Thiamine HCl 4.94071e-05 g/l (DL)-alpha-Lipoic acid 4.94071e-05 g/l p-Aminobenzoic acid 4.94071e-05 g/l Riboflavin 4.94071e-05 g/l Nicotinic acid 4.94071e-05 g/l Calcium D-(+)-pantothenate 4.94071e-05 g/l Biotin 1.97628e-05 g/l Folic acid 1.97628e-05 g/l Na2WO4 x 2 H2O 3.95257e-06 g/l Na2SeO3 x 5 H2O 2.96443e-06 g/l Vitamin B12 9.88142e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
16431	positive	growth	80	hyperthermophilic
67770	positive	growth	80	hyperthermophilic

Physiology and metabolism

Oxygen tolerance

16431

Oxygen toleranceanaerobe

Spore formation

@ref	Spore formation	Confidence
125439		96.6

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
16431	hot marine sediment	Kolbeinsey Ridge	Iceland	ISL	Europe	67.0961	-18.7114 67.0961/-18.7114
67770	Submarine hydrothermal system at the Kolbeinsei RIdgeissn

Taxonmaps

69479

Global distribution of 16S sequence FJ766848 (>99% sequence identity) for Methanocaldococcus villosus subclade from Microbeatlas

Aquatic Samples	20
Soil Samples
Animal Samples
Plant Samples
Total Samples	20

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16431	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM37180v1 assembly for Methanocaldococcus villosus KIN24-T80	contig	GCA_000371805	1069083.4	2517093008	1069083	77.68
66792	MethanoVillo1.0 assembly for Methanocaldococcus villosus KIN24-T80	contig	GCA_000363885	1069083.5	2554235511	1069083	51.8

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
16431	Methanocaldococcus villosus strain KIN24-T80 16S ribosomal RNA gene, partial sequence	FJ766848	1378		1069083

GC content

@ref	GC-content (mol%)	Method
16431	30	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Methanocaldococcus villosus KIN24-T80
NCBI: GCA_000363885

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	88.60	no
125439	gram_stain	BacteriaNetⓘ	variable	77.50	no
125439	motility	BacteriaNetⓘ	yes	87.70	no
125439	spore_formation	BacteriaNetⓘ	yes	96.60	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Biotechnology	Method for automated high performance closed batch cultivation of gas-utilizing methanogens.	Hofmann W, Orthofer M, Salas Wallach N, Ruddyard A, Ungerank M, Paulik C, Rittmann SKR.	AMB Express	10.1186/s13568-025-01872-y	2025
Biotechnology	Quantitative analysis of amino acid excretion by Methanothermobacter marburgensis under N₂-fixing conditions.	Reischl B, Schupp B, Palabikyan H, Steger-Mahnert B, Fink C, Rittmann SKR.	Sci Rep	10.1038/s41598-025-87686-1	2025
Biotechnology	Lipidomics and Comparative Metabolite Excretion Analysis of Methanogenic Archaea Reveal Organism-Specific Adaptations to Varying Temperatures and Substrate Concentrations.	Taubner RS, Baumann LMF, Steiner M, Pfeifer K, Reischl B, Korynt K, Bauersachs T, Mahnert B, Clifford EL, Peckmann J, Schuster B, Birgel D, Rittmann SKR.	mSystems	10.1128/msystems.01159-22	2023
	Method for Indirect Quantification of CH4 Production via H2O Production Using Hydrogenotrophic Methanogens.	Taubner RS, Rittmann SK.	Front Microbiol	10.3389/fmicb.2016.00532	2016
Genetics	Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).	Thennarasu S, Polireddy D, Antony A, Yada MR, Algarawi S, Sivakumar N	Genome Announc	10.1128/genomeA.00481-13	2013
Phylogeny	Methanocaldococcus villosus sp. nov., a heavily flagellated archaeon that adheres to surfaces and forms cell-cell contacts.	Bellack A, Huber H, Rachel R, Wanner G, Wirth R	Int J Syst Evol Microbiol	10.1099/ijs.0.023663-0	2010

References

#16431	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22612
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanocaldococcus villosus (DSM 22612, JCM 16315, KIN24-T80)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000371805

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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