Weissella minor (CCUG 30668, DSM 20014, ATCC 35412)

Name: Weissella minor (CCUG 30668, DSM 20014, ATCC 35412)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6847

Type strain

Strain Designation 3

Culture col. no. CCUG 30668 DSM 20014 ATCC 35412 3 NRIC 1625 9 more

NCBI tax ID(s) 1620

Special Collections DSMZ CCUG JCM KCTC CIP

History <- I. G. Abo-Elnaga, 3 (Lactobacillus viridescens subsp. minor) T. Mitsuoka S8-21 <-- DSM 20014 <-- I. G. Abo-Elnaga 3 ("Lactobacillus viridescens subsp. minor"). CIP <- 1987, NCFB, Lactobacillus minor <- 1970, O. Kandler: strain 3, Lactobacillus viridesens subsp. minor

Links

Weissella minor 3 is an anaerobe, Gram-positive, oval-shaped bacterium that was isolated from milking machine slime.

Gram-positive oval-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 92288

LMG 9847,ATCC 35412,DSM 20014,NCFB 1973,CCUG 30668,CIP 102978,JCM 1168,NCDO 1973,CCRC 14049,CECT 572,NCFB2780,NCIMB 701973,NCIMB 702780,CECT 4708,KCTC 3604,BCRC 14049

@ref 20215

Last LPSN update 2026-02-09 11:19:20

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Weissella

Species Weissella minor

Full scientific name Weissella minor (Kandler et al. 1983 ex Abo-Elnaga and Kandler 1965) Collins et al. 1994

Synonyms (2)

"Lactobacillus viridescens subsp. minor"
Lactobacillus minor

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
120961	positive	oval-shaped
125438			92.5
125438	positive		95.817

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8427	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
38113	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
120961	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
8427	positive	growth	30
38113	positive	growth	30
50205	positive	growth	30
67770	positive	growth	30
120961	positive	growth	10-37
120961	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
50205	anaerobe
120961	facultative anaerobe
125439	facultative anaerobe	92.6

Halophily

@ref	Salt	Growth	Tested relation	Concentration
120961	NaCl	positive	growth	0-8 %
120961	NaCl		growth	10 %

Murein

@ref	Murein short key	Type
8427	A11.20	A3alpha L-Lys-L-Ser-L-Ala₂

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
120961	16947 ChEBI	citrate	-	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	+	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	+	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	+	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
120961	17632 ChEBI	nitrate	-	reduction
120961	17632 ChEBI	nitrate	+	respiration
120961	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
120961	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Metabolite	Production
120961	polysaccharides

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
120961	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120961	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120961	caseinase	-	3.4.21.50
120961	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
120961	gelatinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
120961	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120961	ornithine decarboxylase	-	4.1.1.17
120961	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

Fatty acid profile

Metadata FA analysis

@ref

50205

Fatty acid	Percentage	ECL
C18:1 ω7c /12t/9t	33.9	17.824
C18:1 ω9c	22.4	17.769
C16:1 ω7c	19.3	15.819
C16:0	10.3	16
C16:1 ω7c/C15:0 ISO 2OH	4.2	15.85
C20:1 ω9c	3.2	19.77
C14:0	1.4	14
Unidentified	1.1	18.189
C18:0	1.1	18
C19:1 ISO I	1.1	18.473
Unidentified	0.8	18.27
C13:0 ISO 2OH	0.7	13.814
C17:1 ω5c ISO	0.6	16.461

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120961	-	+	+	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
8427	-	-	-	-	-	+	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+	-	+	+	-	-	+	+	-	+	-	-	-	-	+	-	-	-	-	-	+	-	+	-	-
120961	not determinedn.d.	-	-	-	-	+	-	-	-	-	+/-	+	+	+	-	-	-	-	+/-	-	-	-	+	-	-	+	-	+	+	-	-	+	+	-	+	-	-	-	-	+	-	-	-	-	-	+	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Engineered product

Isolation

@ref	Sample type
8427	milking machine slime
50205	Milking machine slime
67770	Milking machine slime
120961	Food, Milking machine sludge

Taxonmaps

69479

Global distribution of 16S sequence M23039 (>99% sequence identity) for Weissella from Microbeatlas

Aquatic Samples	293
Soil Samples	398
Animal Samples	2522
Plant Samples	272
Total Samples	3485

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8427	1	Risk group (German classification) According to TRBA 466
120961	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM143742v1 assembly for Weissella minor DSM 20014	scaffold	GCA_001437425	1620.3	2671181108	1620	68.59

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Lactobacillus minor 16S ribosomal RNA	X52569	1514	1620
20218	Weissella minor gene for 16S rRNA, strain:NRIC 1625	AB022920	1478	1620
67770	Weissella minor gene for 16S ribosomal RNA, partial sequence, strain: JCM 1168	LC065034	1514	1620
67770	Lactobacillus minor, 16S small subunit ribosomal RNA	M23039	1524	1620

GC content

@ref	GC-content (mol%)
8427	44.7
67770	39.8

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Weissella minor DSM 20014
NCBI: GCA_001437425

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	83.20	no
125439	motility	BacteriaNetⓘ	yes	78.10	no
125439	gram_stain	BacteriaNetⓘ	positive	79.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	92.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Weissella minor strain DSM 20014
PATRIC: 1620.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	95.82	no
125438	anaerobic	anaerobicⓘ	no	89.08	no
125438	spore-forming	spore-formingⓘ	no	88.37	no
125438	aerobic	aerobicⓘ	no	93.55	no
125438	thermophilic	thermophileⓘ	no	96.00	yes
125438	flagellated	motile2+ⓘ	no	92.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Profiling the gut structure and microbiota, and identifying two dominant bacteria belonging to the Weissella genus in mandarin fish (Siniperca chuatsi) fed an artificial diet.	Wang J, Hao Y, Zhang L, Gao X, Xu Y, Wang J, Hanafiah F, Khor W, Sun Y, Wu C.	Front Microbiol	10.3389/fmicb.2024.1486501	2024
	Comparison of Real-Time PCR and Droplet Digital PCR for the Quantitative Detection of Lactiplantibacillus plantarum subsp. plantarum	Choi CH, Kim E, Yang SM, Kim DS, Suh SM, Lee GY, Kim HY.	Foods	10.3390/foods11091331	2022
Pathogenicity	Biological activities and structural properties of the atypical bacteriocins mesenterocin 52b and leucocin b-ta33a.	Corbier C, Krier F, Mulliert G, Vitoux B, Revol-Junelles AM.	Appl Environ Microbiol	10.1128/aem.67.4.1418-1422.2001	2001
	Detection and characterization of Lactobacillus spp. in the porcine seminal plasma and their influence on boar semen quality.	Schulze M, Schafer J, Simmet C, Jung M, Gabler C.	PLoS One	10.1371/journal.pone.0202699	2018
	Nucleotide sequence of 16S ribosomal RNA from Lactobacillus kandleri and Lactobacillus minor.	Martinez-Murcia AJ, Collins MD.	Nucleic Acids Res	10.1093/nar/18.11.3401	1990
Phylogeny	Weissella uvarum sp. nov., isolated from wine grapes.	Nisiotou A, Dourou D, Filippousi ME, Banilas G, Tassou C	Int J Syst Evol Microbiol	10.1099/ijs.0.066209-0	2014
Phylogeny	Weissella ceti sp. nov., isolated from beaked whales (Mesoplodon bidens).	Vela AI, Fernandez A, Bernaldo de Quiros Y, Herraez P, Dominguez L, Fernandez-Garayzabal JF	Int J Syst Evol Microbiol	10.1099/ijs.0.028522-0	2011

References

#8427	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20014
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#38113	; Curators of the CIP;
#50205	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 30668
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120961	Collection of Institut Pasteur ; Curators of the CIP; CIP 102978
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Weissella minor (CCUG 30668, DSM 20014, ATCC 35412)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Murein

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

Fatty acid profile

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001437425

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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