Name: Weissella minor 3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6847

Type strain:

Species: Weissella minor

Strain Designation: 3

Culture col. no.: CCUG 30668, DSM 20014, ATCC 35412, JCM 1168, BCRC 14049, CECT 572, CIP 102978, KCTC 3604, LMG 9847, NCFB 1973, NCIMB 702780, NRIC 1625, NCDO 1973

Strain history: CIP <- 1987, NCFB, Lactobacillus minor <- 1970, O. Kandler: strain 3, Lactobacillus viridesens subsp. minor

NCBI tax ID(s): 1620 (species)

SI-ID 92288

LMG 9847, ATCC 35412, DSM 20014, NCFB 1973, CCUG 30668, CIP 102978, JCM 1168, NCDO 1973, CCRC 14049, CECT 572, NCFB2780, NCIMB 701973, NCIMB 702780, CECT 4708, KCTC 3604, BCRC 14049

Section

Weissella minor 3 is an anaerobe, mesophilic bacterium that was isolated from milking machine slime.

anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Weissella*
	Species	**Weissella minor**
	Full Scientific Name (LPSN)	Weissella minor (Kandler et al. 1983 ex Abo-Elnaga and Kandler 1965) Collins et al. 1994

	Synonym	"Lactobacillus* viridescens subsp. minor"*
	Synonym	Lactobacillus minor

Information on morphological and physiological properties Morphology

[Ref.: #120961]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #120961]	Cell shape	oval-shaped

[Ref.: #120961]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %97.059

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8427]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #8427]

Culture medium growth

[Ref.: #8427]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8427]

Culture medium composition

expand / minimize

[Ref.: #38113]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #38113]

Culture medium growth

[Ref.: #38113]

Culture medium composition

expand / minimize

[Ref.: #120961]

Culture medium

CIP Medium 40

[Ref.: #120961]

Culture medium growth

[Ref.: #120961]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #8427]		growth	30 °C
[Ref.: #38113]		growth	30 °C
[Ref.: #50205]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #120961]		growth	10-37 °C
[Ref.: #120961]	no	growth	45 °C

[Ref.: #8427]	Temperature range	mesophilic
[Ref.: #38113]	Temperature range	mesophilic
[Ref.: #50205]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #120961]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #50205]	Oxygen tolerance	anaerobe
[Ref.: #120961]	Oxygen tolerance	facultative anaerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.99

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #120961]		NaCl		growth	0-8	%
[Ref.: #120961]		NaCl	no	growth	10	%

[Ref.: #8427]	Murein short key	A11.20
[Ref.: #8427]	Murein types	A3alpha L-Lys-L-Ser-L-Ala₂

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #120961]	citrate ChEBI	-	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #120961]	nitrate ChEBI	-	reduction
[Ref.: #120961]	nitrate ChEBI	+	respiration
[Ref.: #120961]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #120961]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #120961]		polysaccharides	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #120961]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120961]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120961]	caseinase	-	3.4.21.50
[Ref.: #120961]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #120961]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120961]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120961]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120961]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #50205]

Fatty acid	Percentage	ECL
C18:1 ω7c /12t/9t	33.9	17.824
C18:1 ω9c	22.4	17.769
C16:1 ω7c	19.3	15.819
C16:0	10.3	16
C16:1 ω7c/C15:0 ISO 2OH	4.2	15.85
C20:1 ω9c	3.2	19.77
C14:0	1.4	14
Unidentified	1.1	18.189
C18:0	1.1	18
C19:1 ISO I	1.1	18.473
Unidentified	0.8	18.27
C13:0 ISO 2OH	0.7	13.814
C17:1 ω5c ISO	0.6	16.461

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #8427]	14434	-	-	-	-	-	+	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+	-	+	+	-	-	+	+	-	+	-	-	-	-	+	-	-	-	-	-	+	-	+	-	-
[Ref.: #120961]	9115	not determinedn.d.	-	-	-	-	+	-	-	-	-	+/-	+	+	+	-	-	-	-	+/-	-	-	-	+	-	-	+	-	+	+	-	-	+	+	-	+	-	-	-	-	+	-	-	-	-	-	+	-	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120961]	7287	-	+	+	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8427]	Sample type/isolated from	milking machine slime

[Ref.: #50205]	Sample type/isolated from	Milking machine slime

[Ref.: #67770]	Sample type/isolated from	Milking machine slime

[Ref.: #120961]	Sample type/isolated from	Food, Milking machine sludge
* marker position based on {}

Isolation sources categories

#Engineered

#Industrial

#Engineered product

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence M23039 (>99% sequence identity) for Weissella from Microbeatlas

Aquatic Samples	293
Soil Samples	398
Animal Samples	2522
Plant Samples	272
Total Samples	3485

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8427]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120961]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Weissella minor gene for 16S ribosomal RNA, partial sequence, strain: JCM 1168	LC065034	1514	ENA	1620 tax ID
[Ref.: #67770]	Lactobacillus minor, 16S small subunit ribosomal RNA	M23039	1524	ENA	1620 tax ID
[Ref.: #20218]	Lactobacillus minor 16S ribosomal RNA	X52569	1514	ENA	1620 tax ID	*
[Ref.: #20218]	Weissella minor gene for 16S rRNA, strain:NRIC 1625	AB022920	1478	ENA	1620 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Weissella minor DSM 20014	GCA_001437425	scaffold	GenBank	1620 tax ID
[Ref.: #66792]	Weissella minor strain DSM 20014	1620.3	wgs	PATRIC	1620 tax ID	*
[Ref.: #66792]	Weissella minor DSM 20014	2671181108	draft	JGI IMG/M	1620 tax ID	*

[Ref.: #8427]	GC-content	44.7 mol%
[Ref.: #67770]	GC-content	39.8 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Weissella minor DSM 20014 NCBI: GCA_001437425.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Weissella minor strain DSM 20014 PATRIC: 1620.3
[Ref.: #69480]	motile	no	96.253	no
[Ref.: #69480]	gram-positive	yes	96.515	no
[Ref.: #69480]	anaerobic	no	92.021	yes
[Ref.: #69480]	halophile	yes	93.536	no
[Ref.: #69480]	spore-forming	no	95.847	no
[Ref.: #69480]	thermophile	no	99.815	no
[Ref.: #69480]	glucose-util	yes	91.594	no
[Ref.: #69480]	flagellated	no	98.362	no
[Ref.: #69480]	aerobic	no	94.752	yes
[Ref.: #69480]	glucose-ferment	yes	90.559	no

Availability in culture collections External links

[Ref.: #8427]

Culture collection no.

CCUG 30668, DSM 20014, ATCC 35412, JCM 1168, BCRC 14049, CECT 572, CIP 102978, KCTC 3604, LMG 9847, NCFB 1973, NCIMB 702780, NRIC 1625, NCDO 1973

[Ref.: #76312]

SI-ID 92288

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Weissella uvarum sp. nov., isolated from wine grapes.	Nisiotou A, Dourou D, Filippousi ME, Banilas G, Tassou C	Int J Syst Evol Microbiol	10.1099/ijs.0.066209-0	2014	*
Phylogeny	Weissella ceti sp. nov., isolated from beaked whales (Mesoplodon bidens).	Vela AI, Fernandez A, Bernaldo de Quiros Y, Herraez P, Dominguez L, Fernandez-Garayzabal JF	Int J Syst Evol Microbiol	10.1099/ijs.0.028522-0	2011	*

References

#8427

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20014

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38113 ; Curators of the CIP;
#50205 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 30668

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76312

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID92288.1 )

#120961 Collection of Institut Pasteur ; Curators of the CIP; CIP 102978
* These data were automatically processed and therefore are not curated

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Weissella minor DSM 20014

Weissella minor strain DSM 20014

Change proposal

Change proposal