Hellea balneolensis 26III/A02/215 | Type strain | DSM 19091, CIP 109500, OOB 269 | BacDiveID:6220

Strain identifier

BacDive ID: 6220

Type strain:

Species: Hellea balneolensis

Strain Designation: 26III/A02/215

Culture col. no.: DSM 19091, CIP 109500, OOB 269

Strain history: CIP <- 2008 <- K. Alain, Paris VI Univ. CNRS, UMR 7621, Banyuls sur mer, France: strain 26III/A02/215, "Eidothea erythraea"

NCBI tax ID(s): 1121948 (strain), 287478 (species)

SI-ID 360132

DSM 19091, CIP 109500

Section

Hellea balneolensis 26III/A02/215 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from surface microlayer of coastal water.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Caulobacterales*
	Family	*Hyphomonadaceae*
	Genus	*Hellea*
	Species	**Hellea balneolensis**
	Full Scientific Name (LPSN)	Hellea balneolensis Alain et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #32393]	Gram stain	negative
[Ref.: #116717]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.807

[Ref.: #32393]	Cell length	2.7-5.6 µm

[Ref.: #32393]	Cell width	0.28-0.48 µm

[Ref.: #32393]	Cell shape	rod-shaped
[Ref.: #116717]	Cell shape	rod-shaped

[Ref.: #32393]	Motility	yes
[Ref.: #116717]	Motility	no

Pigment production

yes

Multimedia content

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Information on culture and growth conditions Culture and growth conditions

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

Culture medium

Marine agar (MA)

Culture medium growth

Culture medium composition

expand / minimize

[Ref.: #116717]

Culture medium

CIP Medium 13

[Ref.: #116717]

Culture medium growth

[Ref.: #116717]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #7882]			growth	28 °C
[Ref.: #32393]			growth	15-37 °C
[Ref.: #32393]			optimum	30 °C

[Ref.: #7882]	Temperature range	mesophilic
[Ref.: #32393]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #32393]			optimum	7
[Ref.: #32393]			growth	06-08

Information on physiology and metabolism Physiology and metabolism

Oxygen tolerance

aerobe

[Ref.: #32393]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32393]		NaCl		growth	0.02-5	%
[Ref.: #32393]		NaCl		optimum	3	%

Observation

aggregates in chains

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32393]		acetate ChEBI	+	carbon source
[Ref.: #32393]		alanine ChEBI	+	carbon source
[Ref.: #32393]		aspartate ChEBI	+	carbon source
[Ref.: #32393]		citrate ChEBI	+	carbon source
[Ref.: #32393]		esculin ChEBI	+	hydrolysis
[Ref.: #32393]		glutamate ChEBI	+	carbon source
[Ref.: #32393]		histidine ChEBI	+	carbon source
[Ref.: #116717]		nitrate ChEBI	-	reduction
[Ref.: #116717]		nitrite ChEBI	-	reduction
[Ref.: #32393]		proline ChEBI	+	carbon source
[Ref.: #32393]		propionate ChEBI	+	carbon source
[Ref.: #32393]		pyruvate ChEBI	+	carbon source
[Ref.: #32393]		succinate ChEBI	+	carbon source
		Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #116717]		indole ChEBI	no

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #68382]		acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #32393]		alkaline phosphatase	+	3.1.3.1
[Ref.: #68382]		alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]		alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]		alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]		alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]		alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]		alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]		beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]		beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]		beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #32393]		catalase	+	1.11.1.6
[Ref.: #116717]		catalase	+	1.11.1.6
[Ref.: #68382]		cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]		esterase (C 4)	+		* from API zym
[Ref.: #68382]		esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]		leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]		lipase (C 14)	-		* from API zym
[Ref.: #68382]		N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]		naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #116717]		oxidase	-
[Ref.: #68382]		trypsin	-	3.4.21.4	* from API zym
[Ref.: #116717]		urease	-	3.5.1.5
[Ref.: #68382]		valine arylamidase	+		* from API zym
		Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116717]	11912	-	+	+	+	-	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7882]	Sample type/isolated from	surface microlayer of coastal water
[Ref.: #7882]	Geographic location (country and/or sea, region)	bay of Banyuls-sur-mer (42° 29' N 3° 08' E)
[Ref.: #7882]	Country	France
[Ref.: #7882]	Country ISO 3 Code	FRA
[Ref.: #7882]	Continent	Europe
[Ref.: #7882]	Geographic location	42.4833°/3.1333°

[Ref.: #116717]	Sample type/isolated from	Environment, Sea
[Ref.: #116717]	Geographic location (country and/or sea, region)	Banyuls sur mer
[Ref.: #116717]	Country	France
[Ref.: #116717]	Country ISO 3 Code	FRA
[Ref.: #116717]	Continent	Europe
[Ref.: #116717]	Isolation date	2002
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Surface water
#Environmental	#Terrestrial	#Coast

What are isolation sources categories?

Global distribution of 16S sequence AY576758 (>99% sequence identity) for Hellea balneolensis subclade from Microbeatlas

Aquatic Samples	14372
Soil Samples	409
Animal Samples	508
Plant Samples	193
Total Samples	15482

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7882]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116717]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7882]	Hellea balneolensis strain 26III/A02/215 16S ribosomal RNA gene, partial sequence	AY576758	1412	ENA	287478 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Hellea balneolensis DSM 19091	GCA_000428525	scaffold	GenBank	1121948 tax ID	*
[Ref.: #66792]	Hellea balneolensis DSM 19091	1121948.5	wgs	PATRIC	1121948 tax ID	*
[Ref.: #66792]	Hellea balneolensis DSM 19091	2524614726	draft	JGI IMG/M	1121948 tax ID	*

[Ref.: #7882]	GC-content	46.8 mol%	high performance liquid chromatography (HPLC)
[Ref.: #32393]	GC-content	46.8 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Hellea balneolensis DSM 19091 DSM 19091 NCBI: GCA_000428525.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Hellea balneolensis DSM 19091 DSM 19091 NCBI: GCA_000428525.1
[Ref.: #69480]	motile	no	66.553	yes
[Ref.: #69480]	flagellated	no	96.264	no
[Ref.: #69480]	gram-positive	no	98.466	yes
[Ref.: #69480]	anaerobic	no	99.436	yes
[Ref.: #69480]	aerobic	yes	93.518	yes
[Ref.: #69480]	halophile	no	59.717	no
[Ref.: #69480]	spore-forming	no	97.576	no
[Ref.: #69480]	glucose-util	yes	67.295	no
[Ref.: #69480]	thermophile	no	98.295	yes
[Ref.: #69480]	glucose-ferment	no	91.716	no

Availability in culture collections External links

Culture collection no.

DSM 19091, CIP 109500, OOB 269

SI-ID 360132

*

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Algimonas porphyrae gen. nov., sp. nov., a member of the family Hyphomonadaceae, isolated from the red alga Porphyra yezoensis.	Fukui Y, Abe M, Kobayashi M, Saito H, Oikawa H, Yano Y, Satomi M	Int J Syst Evol Microbiol	10.1099/ijs.0.040485-0	2012	*
Phylogeny	Litorimonas taeanensis gen. nov., sp. nov., isolated from a sandy beach.	Jung JY, Kim JM, Jin HM, Kim SY, Park W, Jeon CO	Int J Syst Evol Microbiol	10.1099/ijs.0.022129-0	2010	*
Phylogeny	Hellea balneolensis gen. nov., sp. nov., a prosthecate alphaproteobacterium from the Mediterranean Sea.	Alain K, Tindall BJ, Intertaglia L, Catala P, Lebaron P	Int J Syst Evol Microbiol	10.1099/ijs.0.65424-0	2008	*

References

#7882

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19091

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32393

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28617 (see below)

#36077 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#75699

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID360132.1 )

#116717 Collection of Institut Pasteur ; Curators of the CIP; CIP 109500

#28617

IJSEM 2511 2008 ( DOI 10.1099/ijs.0.65424-0 , PubMed 18984685 )

* These data were automatically processed and therefore are not curated

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