Alkalihalobacillus alcalophilus (DSM 485, ATCC 27647, JCM 5262)

Name: Alkalihalobacillus alcalophilus (DSM 485, ATCC 27647, JCM 5262)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 572

Type strain

Strain Designation 1

Culture col. no. DSM 485 ATCC 27647 JCM 5262 NCIB 10436 NCIB 8772 19 more

NCBI tax ID(s) 1445

Special Collections DSMZ CCUG JCM KCTC CIP Literature strains

History DSMZ <- ATCC <- NCTC <- A. Vedder, strain 1 JCM <-- DSM 485 <-- ATCC 27647 <-- NCTC 4553 <-- A. Vedder strain 1. CIP <- 1990, DSM <- ATCC <- NCTC <- A. Vedder: strain 1

Links

Alkalihalobacillus alcalophilus 1 is an obligate aerobe, spore-forming, Gram-positive bacterium that was isolated from human faeces.

spore-forming Gram-positive motile rod-shaped obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 10532

LMG 17938,ATCC 27647,DSM 485,LMG 7120,NCIB 10436,NCIB 8772,NCTC 4553,NCIB 9772,CCUG 28516,NRRL B-14309,CIP 103410,JCM 5262,CCRC 15419,IFO 15653,KCTC 1824,NCIMB 10436,NCIMB 8772,IAM12461,CECT 1,NRRL NRS-1584,VTT E-87298,NBRC 15653,NRRL NRS-1548,BCRC 15419,CCT 0901,KCTC 3884,CGMCC 1.3604

@ref 20215

Last LPSN update 2026-05-29 08:25:03

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Alkalihalobacillus

Species Alkalihalobacillus alcalophilus

Full scientific name Alkalihalobacillus alcalophilus (Vedder 1934) Patel and Gupta 2020

Synonyms (1)

Bacillus alcalophilus

BacDive ID	Other strains from **Alkalihalobacillus alcalophilus** (4)	Type strain
573	A. alcalophilus 2, DSM 2526, NCIB 10438
574	A. alcalophilus PN 139, DP 486, DSM 9707
575	A. alcalophilus PN 101, JA 16, DSM 9708
576	A. alcalophilus PN 138, DP 469, DSM 9709

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
23138	positive	rod-shaped
116209	positive	rod-shaped

Colony morphology

@ref	Colony size	Colony color	Incubation period
23138	2.3 mm	white	2 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
34261	MEDIUM 113 - for Bacillus		Distilled water make up to (900.000 ml);Columbia agar (39.000 g);Solution 1 - M00343 (100.000 ml)
226	ALKALINE NUTRIENT AGAR (DSMZ Medium 31)	Medium recipe at MediaDive	Name: ALKALINE NUTRIENT AGAR (DSMZ Medium 31) Composition: Agar 15.0 g/l Na2CO3 anhydrous 5.3 g/l Peptone 5.0 g/l NaHCO3 4.2 g/l Meat extract 3.0 g/l Distilled water
116209	CIP Medium 113	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
226	positive	growth	37
23138	positive	maximum	46
34261	positive	growth	30
67770	positive	growth	30
116209	positive	growth	30
116209	negative	growth	10
116209	negative	growth	45
116209	negative	growth	55

Culture pH

@ref	Ability	Type	PH
116209		growth	6

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
116209	obligate aerobe
125439	facultative anaerobe	93.814

Spore formation

@ref	Spore description	Type of spore	Confidence
23138	terminal to subterminal	endospore
116209
125438			94.791
125439			90.349

Compound production

226

Compoundprotease, alkaline

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23138	NaCl		growth	>0 %
116209	NaCl	positive	growth	0-6 %
116209	NaCl		growth	8 %
116209	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68368	27613 ChEBI	amygdalin	-	fermentation	from API 20E
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
23138		casein	+	hydrolysis
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
116209	16947 ChEBI	citrate	-	carbon source
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
23138	17634 ChEBI	D-glucose	-	builds gas from
23138	17634 ChEBI	D-glucose	+	fermentation
68371	17634 ChEBI	D-glucose	-	builds acid from	from API 50CH acid
68368	17634 ChEBI	D-glucose	-	fermentation	from API 20E
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
23138	16899 ChEBI	D-mannitol	-	builds gas from
23138	16899 ChEBI	D-mannitol	+	fermentation
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68368	16899 ChEBI	D-mannitol	-	fermentation	from API 20E
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
23138	65327 ChEBI	D-xylose	-	builds gas from
23138	65327 ChEBI	D-xylose	+	fermentation
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
23138	5291 ChEBI	gelatin	+	hydrolysis
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
23138	17754 ChEBI	glycerol	-	builds gas from
23138	17754 ChEBI	glycerol	+	fermentation
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
23138	30849 ChEBI	L-arabinose	-	builds gas from
23138	30849 ChEBI	L-arabinose	+	fermentation
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68368	30849 ChEBI	L-arabinose	-	fermentation	from API 20E
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68368	62345 ChEBI	L-rhamnose	-	fermentation	from API 20E
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
23138	17716 ChEBI	lactose	-	builds gas from
23138	17716 ChEBI	lactose	+	fermentation
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68368	25094 ChEBI	lysine	-	degradation	from API 20E
23138	17306 ChEBI	maltose	-	builds gas from
23138	17306 ChEBI	maltose	+	fermentation
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68368	28053 ChEBI	melibiose	-	fermentation	from API 20E
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
23138	6872 ChEBI	methylene blue	+	reduction
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68368	17268 ChEBI	myo-inositol	-	fermentation	from API 20E
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
116209	17632 ChEBI	nitrate	-	reduction
116209	17632 ChEBI	nitrate	-	respiration
116209	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	+	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
23138	17814 ChEBI	salicin	-	builds gas from
23138	17814 ChEBI	salicin	+	fermentation
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
23138	30911 ChEBI	sorbitol	-	builds gas from
23138	30911 ChEBI	sorbitol	+	fermentation
68368	30911 ChEBI	sorbitol	-	fermentation	from API 20E
23138	28017 ChEBI	starch	+	hydrolysis
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
23138	17992 ChEBI	sucrose	-	builds gas from
23138	17992 ChEBI	sucrose	+	fermentation
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68368	17992 ChEBI	sucrose	-	fermentation	from API 20E
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
23138	27897 ChEBI	tryptophan	+	energy source
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite
23138	15688 ChEBI	acetoin
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
23138	35581 ChEBI	indole
116209	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
23138	15688 ChEBI	acetoin	-
116209	15688 ChEBI	acetoin	-
68368	15688 ChEBI	acetoin	+		from API 20E
23138	35581 ChEBI	indole		+
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
116209	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
116209	amylase	+
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68382	beta-galactosidase	+	3.2.1.23	from API zym
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
116209	caseinase	-	3.4.21.50
116209	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68368	cytochrome oxidase	-	1.9.3.1	from API 20E
116209	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
116209	gelatinase	+
68368	gelatinase	+		from API 20E
116209	lecithinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
116209	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
116209	ornithine decarboxylase	-	4.1.1.17
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
116209	oxidase	+
116209	protease	-
68382	trypsin	-	3.4.21.4	from API zym
23138	tryptophan deaminase	+	4.1.99.1
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
23138	urease	+	3.5.1.5
116209	urease	-	3.5.1.5
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	-		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	threonine metabolism	100	10 of 10
66794	adipate degradation	100	2 of 2
66794	formaldehyde oxidation	100	3 of 3
66794	pentose phosphate pathway	100	11 of 11
66794	taurine degradation	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	teichoic acid biosynthesis	100	1 of 1
66794	L-lactaldehyde degradation	100	3 of 3
66794	sulfopterin metabolism	100	4 of 4
66794	lipoate biosynthesis	100	5 of 5
66794	ribulose monophosphate pathway	100	2 of 2
66794	ketogluconate metabolism	100	8 of 8
66794	aerobactin biosynthesis	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	methylglyoxal degradation	100	5 of 5
66794	palmitate biosynthesis	95.45	21 of 22
66794	tetrahydrofolate metabolism	92.86	13 of 14
66794	vitamin B1 metabolism	92.31	12 of 13
66794	Entner Doudoroff pathway	90	9 of 10
66794	serine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	propanol degradation	85.71	6 of 7
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	photosynthesis	85.71	12 of 14
66794	methionine metabolism	84.62	22 of 26
66794	phenylalanine metabolism	84.62	11 of 13
66794	1,4-dihydroxy-6-naphthoate biosynthesis	83.33	5 of 6
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	glycogen metabolism	80	4 of 5
66794	propionate fermentation	80	8 of 10
66794	purine metabolism	78.72	74 of 94
66794	citric acid cycle	78.57	11 of 14
66794	heme metabolism	78.57	11 of 14
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	allantoin degradation	77.78	7 of 9
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	leucine metabolism	76.92	10 of 13
66794	pyrimidine metabolism	75.56	34 of 45
66794	ppGpp biosynthesis	75	3 of 4
66794	gluconeogenesis	75	6 of 8
66794	isoleucine metabolism	75	6 of 8
66794	butanoate fermentation	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	biotin biosynthesis	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	degradation of sugar alcohols	75	12 of 16
66794	peptidoglycan biosynthesis	73.33	11 of 15
66794	flavin biosynthesis	73.33	11 of 15
66794	vitamin B6 metabolism	72.73	8 of 11
66794	NAD metabolism	72.22	13 of 18
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	glycolysis	70.59	12 of 17
66794	starch degradation	70	7 of 10
66794	alanine metabolism	68.97	20 of 29
66794	histidine metabolism	68.97	20 of 29
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	cyanate degradation	66.67	2 of 3
66794	d-mannose degradation	66.67	6 of 9
66794	sulfoquinovose degradation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	arginine metabolism	66.67	16 of 24
66794	octane oxidation	66.67	2 of 3
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	non-pathway related	65.79	25 of 38
66794	oxidative phosphorylation	63.74	58 of 91
66794	d-xylose degradation	63.64	7 of 11
66794	proline metabolism	63.64	7 of 11
66794	tryptophan metabolism	63.16	24 of 38
66794	isoprenoid biosynthesis	61.54	16 of 26
66794	urea cycle	61.54	8 of 13
66794	factor 420 biosynthesis	60	3 of 5
66794	glycine betaine biosynthesis	60	3 of 5
66794	cellulose degradation	60	3 of 5
66794	gallate degradation	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	myo-inositol biosynthesis	60	6 of 10
66794	degradation of aromatic, nitrogen containing compounds	58.33	7 of 12
66794	lipid metabolism	58.06	18 of 31
66794	polyamine pathway	56.52	13 of 23
66794	degradation of sugar acids	56	14 of 25
66794	phenol degradation	55	11 of 20
66794	sulfate reduction	53.85	7 of 13
66794	kanosamine biosynthesis II	50	1 of 2
66794	glycine metabolism	50	5 of 10
66794	pantothenate biosynthesis	50	3 of 6
66794	phenylmercury acetate degradation	50	1 of 2
66794	coenzyme A metabolism	50	2 of 4
66794	tyrosine metabolism	50	7 of 14
66794	degradation of pentoses	50	14 of 28
66794	ethanol fermentation	50	1 of 2
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	CDP-diacylglycerol biosynthesis	50	1 of 2
66794	cysteine metabolism	50	9 of 18
66794	glutathione metabolism	50	7 of 14
66794	lysine metabolism	47.62	20 of 42
66794	ascorbate metabolism	45.45	10 of 22
66794	metabolism of disaccharids	45.45	5 of 11
66794	nitrate assimilation	44.44	4 of 9
66794	degradation of hexoses	44.44	8 of 18
66794	cardiolipin biosynthesis	42.86	3 of 7
66794	creatinine degradation	40	2 of 5
66794	phenylacetate degradation (aerobic)	40	2 of 5
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	phosphatidylethanolamine bioynthesis	38.46	5 of 13
66794	dTDPLrhamnose biosynthesis	37.5	3 of 8
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	coenzyme M biosynthesis	30	3 of 10
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	toluene degradation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	bile acid biosynthesis, neutral pathway	23.53	4 of 17
66794	lipid A biosynthesis	22.22	2 of 9
66794	arachidonic acid metabolism	22.22	4 of 18
66794	4-hydroxymandelate degradation	22.22	2 of 9

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
49264	+	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
116209	-	+	+	+	-	-	-	-	-	-	-	-	+	+	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
49264	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
116209	not determinedn.d.	+	+	+	+	+	-	+	+	+	+	+	+	+	+	-	+	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	+	-	+	+	+	-	-	-	-	+	+	-	-	-	-	-	-	-	+	+	-	-	+	-	-	-	-	+	+	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	+	+	-	+	+	-	-	-	-	-	+	-	+	+	-	+	+	+	+	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Human	-
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

Isolation

@ref	Sample type	Host species
226	human faeces	Homo sapiens
49264	Human feces	Homo sapiens
67770	Human feces	Homo sapiens
116209	Human, Faeces	Homo sapiens

Taxonmaps

69479

Global distribution of 16S sequence X76436 (>99% sequence identity) for Alkalihalobacillus alcalophilus subclade from Microbeatlas

Aquatic Samples	25
Soil Samples	53
Animal Samples	80
Plant Samples	8
Total Samples	166

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
226	1	Risk group (German classification) According to TRBA 466
116209	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM29224v2 assembly for Alkalihalobacillus alcalophilus ATCC 27647 = CGMCC 1.3604 AV1934	contig	GCA_000292245	1218173.3	2519899572	1218173	38.68
67770	ASM480251v1 assembly for Alkalihalobacillus alcalophilus ATCC 27647 = CGMCC 1.3604	scaffold	GCA_004802515	1218173.9		1218173	0

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	B.alcalophilus 16S ribosomal RNA	X60603	1430	1218173
20218	B.alcalophilus (DSM 485 T) gene for 16S rRNA	X76436	1505	1218173
20218	Bacillus alcalophilus gene for 16S rRNA, partial sequence	AB006919	274	1218173
20218	Bacillus alcalophilus gene for 16S rRNA, partial sequence, strain: NBRC 15653	AB363730	1476	1218173

GC content

@ref	GC-content (mol%)	Method
226	37.0	thermal denaturation, midpoint method (Tm)
226	36.7	Buoyant density centrifugation (BD)
67770	37	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM29224v2 assembly for Alkalihalobacillus alcalophilus ATCC 27647 = CGMCC 1.3604 AV1934
BV-BRC: 1218173.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	93.81	no
125439	gram_stain	BacteriaNetⓘ	variable	63.87	no
125439	motility	BacteriaNetⓘ	yes	79.87	no
125439	spore_formation	BacteriaNetⓘ	yes	90.35	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Bacillus alcalophilus ATCC 27647
PATRIC: 1218173.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	79.89	yes
125438	anaerobic	anaerobicⓘ	no	96.81	yes
125438	spore-forming	spore-formingⓘ	yes	94.79	yes
125438	aerobic	aerobicⓘ	yes	70.14	no
125438	thermophilic	thermophileⓘ	no	93.55	yes
125438	flagellated	motile2+ⓘ	yes	85.18	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genomic insights into novel extremotolerant bacteria isolated from the NASA Phoenix mission spacecraft assembly cleanrooms.	Schultz J, Jamil T, Sengupta P, Sivabalan SKM, Rawat A, Patel N, Krishnamurthi S, Alam I, Singh NK, Raman K, Rosado AS, Venkateswaran K.	Microbiome	10.1186/s40168-025-02082-1	2025
	Molecular Structure of Phosphoserine Aminotransferase from Saccharomyces cerevisiae.	Jang J, Chang JH.	Int J Mol Sci	10.3390/ijms24065139	2023
Phylogeny	Diversity of Endolysin Domain Architectures in Bacteriophages Infecting Bacilli.	Koposova ON, Kazantseva OA, Shadrin AM.	Biomolecules	10.3390/biom14121586	2024
Genetics	Genotype-Dependent Response of Root Microbiota and Leaf Metabolism in Olive Seedlings Subjected to Drought Stress.	Azri R, Lamine M, Bensalem-Fnayou A, Hamdi Z, Mliki A, Ruiz-Lozano JM, Aroca R.	Plants (Basel)	10.3390/plants13060857	2024
	H⁺-capacitor and ATP production in obligate alkaliphilic Bacillaceae: insights into cytochrome c and H⁺ transport mechanisms.	Yumoto I.	Front Microbiol	10.3389/fmicb.2025.1637315	2025
	Biochemical characterization of a novel oxidatively stable, halotolerant, and high-alkaline subtilisin from Alkalihalobacillus okhensis Kh10-101^T.	Falkenberg F, Rahba J, Fischer D, Bott M, Bongaerts J, Siegert P.	FEBS Open Bio	10.1002/2211-5463.13457	2022
Metabolism	Time-restricted feeding ameliorates non-alcoholic fatty liver disease through modulating hepatic nicotinamide metabolism via gut microbiota remodeling.	Feng R, Yang W, Feng W, Huang X, Cen M, Peng G, Wu W, Wang Z, Jing Y, Long T, Liu Y, Li Z, Chang G, Huang K.	Gut Microbes	10.1080/19490976.2024.2390164	2024
Transcriptome	Pectinolytic lyases: a comprehensive review of sources, category, property, structure, and catalytic mechanism of pectate lyases and pectin lyases.	Zheng L, Xu Y, Li Q, Zhu B.	Bioresour Bioprocess	10.1186/s40643-021-00432-z	2021
	Differences in Bioenergetic Metabolism of Obligately Alkaliphilic Bacillaceae Under High pH Depend on the Aeration Conditions.	Goto T, Ogami S, Yoshimume K, Yumoto I.	Front Microbiol	10.3389/fmicb.2022.842785	2022
Metabolism	The electrochemical proton potential of Bacillus alcalophilus.	Hoffmann A, Dimroth P	Eur J Biochem	10.1111/j.1432-1033.1991.tb16304.x	1991
Phylogeny	Taxonomy of alkaliphilic Bacillus strains.	Fritze D, Flossdorf J, Claus D	Int J Syst Bacteriol	10.1099/00207713-40-1-92	1990
	Alkalihalobacterium elongatum gen. nov. sp. nov.: An Antibiotic-Producing Bacterium Isolated From Lonar Lake and Reclassification of the Genus Alkalihalobacillus Into Seven Novel Genera.	Joshi A, Thite S, Karodi P, Joseph N, Lodha T.	Front Microbiol	10.3389/fmicb.2021.722369	2021
Phylogeny	Bacillus suaedae sp. nov., isolated from the stem of Suaeda aralocaspica in north-west China.	Lei RF, Ma Q, Li YQ, Abudourousuli D, Wang S, Rouzi Z, Aosiman M, An DD, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.005362	2022
Phylogeny	Paralkalibacillus indicireducens gen., nov., sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor used for dyeing.	Hirota K, Nishita M, Matsuyama H, Yumoto I	Int J Syst Evol Microbiol	10.1099/ijsem.0.002248	2017
Phylogeny	Polygonibacillus indicireducens gen. nov., sp. nov., an indigo-reducing and obligate alkaliphile isolated from indigo fermentation liquor for dyeing.	Hirota K, Okamoto T, Matsuyama H, Yumoto I	Int J Syst Evol Microbiol	10.1099/ijsem.0.001405	2016
Phylogeny	Bacillus bogoriensis sp. nov., a novel alkaliphilic, halotolerant bacterium isolated from a Kenyan soda lake.	Vargas VA, Delgado OD, Hatti-Kaul R, Mattiasson B	Int J Syst Evol Microbiol	10.1099/ijs.0.63318-0	2005

References

#226	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 485
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#23138	E. W. BOYER, M. B. INGLE, G. D. MERCER: Bacillus alcalophilus subsp. halodurans subsp. nov.: An Alkaline-Amylase-Producing, Alkalophilic Organism. IJSEM 23: 238 - 242 1973 ( DOI 10.1099/00207713-23-3-238 )
#34261	; Curators of the CIP;
#49264	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 28516
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#116209	Collection of Institut Pasteur ; Curators of the CIP; CIP 103410
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Alkalihalobacillus alcalophilus (DSM 485, ATCC 27647, JCM 5262)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Pathway annotation

API 20E

API zym

API 50CHac

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000292245

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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