Name: Alkalihalobacillus alcalophilus 1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 572

Type strain:

Species: Alkalihalobacillus alcalophilus

Strain Designation: 1

Culture col. no.: DSM 485, ATCC 27647, JCM 5262, NCIB 10436, NCIB 8772, NCTC 4553, CCUG 28516, NRRL B-14309, BCRC 15419, CECT 1, CGMCC 1.3604, CIP 103410, IAM 12461, IFO 15653, KCTC 1824, KCTC 3884, LMG 17938, LMG 7120, NBRC 15653, NCIMB 10436, NCIMB 8772, NRRL NRS-1548, VTT E-87298

Strain history: CIP <- 1990, DSM <- ATCC <- NCTC <- A. Vedder: strain 1

NCBI tax ID(s): 1445 (species)

SI-ID 10532

LMG 17938, ATCC 27647, DSM 485, LMG 7120, NCIB 10436, NCIB 8772, NCTC 4553, NCIB 9772, CCUG 28516, NRRL B-14309, CIP 103410, JCM 5262, CCRC 15419, IFO 15653, KCTC 1824, NCIMB 10436, NCIMB 8772, IAM12461, CECT 1, NRRL NRS-1584, VTT E-87298, NBRC 15653, NRRL NRS-1548, BCRC 15419, CCT 0901, KCTC 3884, CGMCC 1.3604

Other strains from Alkalihalobacillus alcalophilus

A. alcalophilus 2, DSM 2526, NCIB 10438
A. alcalophilus PN 139, DP 486, DSM 9707
A. alcalophilus PN 101, JA 16, DSM 9708
A. alcalophilus PN 138, DP 469, DSM 9709

Section

Alkalihalobacillus alcalophilus 1 is an obligate aerobe, spore-forming, thermophilic bacterium that was isolated from human faeces.

Gram-positive
motile
rod-shaped
spore-forming
obligate aerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Alkalihalobacillus*
	Species	**Alkalihalobacillus alcalophilus**
	Full Scientific Name (LPSN)	Alkalihalobacillus alcalophilus (Vedder 1934) Patel and Gupta 2020

Synonym

Bacillus alcalophilus

Information on morphological and physiological properties Morphology

[Ref.: #23138]	Gram stain	positive
[Ref.: #116209]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #23138]	Cell shape	rod-shaped
[Ref.: #116209]	Cell shape	rod-shaped

[Ref.: #23138]	Motility	yes
[Ref.: #116209]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %96.57

[Ref.: #23138]	Colony size	2.3 mm
[Ref.: #23138]	Colony color	white
[Ref.: #23138]	Incubation period	2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #34261]

Culture medium

MEDIUM 113 - for Bacillus

[Ref.: #34261]

Culture medium growth

[Ref.: #34261]

Culture medium composition

expand / minimize

[Ref.: #226]

Culture medium

ALKALINE NUTRIENT AGAR (DSMZ Medium 31)

[Ref.: #226]

Culture medium growth

[Ref.: #226]

Culture medium link

Medium recipe at MediaDive

[Ref.: #226]

Culture medium composition

expand / minimize

[Ref.: #116209]

Culture medium

CIP Medium 113

[Ref.: #116209]

Culture medium growth

[Ref.: #116209]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #226]		growth	37 °C
[Ref.: #23138]		maximum	46 °C
[Ref.: #34261]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #116209]		growth	30 °C
[Ref.: #116209]	no	growth	10 °C
[Ref.: #116209]	no	growth	45 °C
[Ref.: #116209]	no	growth	55 °C

[Ref.: #226]	Temperature range	mesophilic
[Ref.: #23138]	Temperature range	thermophilic
[Ref.: #34261]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #116209]	Temperature range	mesophilic
[Ref.: #116209]	Temperature range	psychrophilic
[Ref.: #116209]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #116209]		no	growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #116209]

Oxygen tolerance

obligate aerobe

[Ref.: #23138]	Spore description	terminal to subterminal
[Ref.: #23138]	Type of spore	endospore
[Ref.: #23138]	Ability of spore formation	yes
[Ref.: #116209]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #226]

Name of produced compound

protease, alkaline

	Salt	Tested relation		Salt conc.
[Ref.: #23138]	NaCl	no	growth	>0	%
[Ref.: #116209]	NaCl		growth	0-6	%
[Ref.: #116209]	NaCl	no	growth	8	%
[Ref.: #116209]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #23138]	casein	+	hydrolysis
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #116209]	citrate ChEBI	-	carbon source
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23138]	D-glucose ChEBI	-	builds gas from
[Ref.: #23138]	D-glucose ChEBI	+	fermentation
[Ref.: #68371]	D-glucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	D-glucose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23138]	D-mannitol ChEBI	-	builds gas from
[Ref.: #23138]	D-mannitol ChEBI	+	fermentation
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23138]	D-xylose ChEBI	-	builds gas from
[Ref.: #23138]	D-xylose ChEBI	+	fermentation
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23138]	gelatin ChEBI	+	hydrolysis
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23138]	glycerol ChEBI	-	builds gas from
[Ref.: #23138]	glycerol ChEBI	+	fermentation
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23138]	L-arabinose ChEBI	-	builds gas from
[Ref.: #23138]	L-arabinose ChEBI	+	fermentation
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23138]	lactose ChEBI	-	builds gas from
[Ref.: #23138]	lactose ChEBI	+	fermentation
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #23138]	maltose ChEBI	-	builds gas from
[Ref.: #23138]	maltose ChEBI	+	fermentation
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23138]	methylene blue ChEBI	+	reduction
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #116209]	nitrate ChEBI	-	reduction
[Ref.: #116209]	nitrate ChEBI	-	respiration
[Ref.: #116209]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23138]	salicin ChEBI	-	builds gas from
[Ref.: #23138]	salicin ChEBI	+	fermentation
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23138]	sorbitol ChEBI	-	builds gas from
[Ref.: #23138]	sorbitol ChEBI	+	fermentation
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #23138]	starch ChEBI	+	hydrolysis
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23138]	sucrose ChEBI	-	builds gas from
[Ref.: #23138]	sucrose ChEBI	+	fermentation
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23138]	tryptophan ChEBI	+	energy source
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #23138]	acetoin ChEBI	no
[Ref.: #23138]	indole ChEBI	yes
[Ref.: #116209]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #23138]	acetoin ChEBI	-
[Ref.: #116209]	acetoin ChEBI	-
[Ref.: #23138]	indole ChEBI		+
[Ref.: #68368]	acetoin ChEBI	+		* from API 20E
[Ref.: #68368]	indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #116209]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #116209]	amylase	+
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API 20E
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116209]	caseinase	-	3.4.21.50
[Ref.: #116209]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #116209]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #116209]	gelatinase	+
[Ref.: #68368]	gelatinase	+		* from API 20E
[Ref.: #116209]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #116209]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #116209]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #116209]	oxidase	+
[Ref.: #116209]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #23138]	tryptophan deaminase	+	4.1.99.1
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #23138]	urease	+	3.5.1.5
[Ref.: #116209]	urease	-	3.5.1.5
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #49264]	5552	+	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #49264]	2976	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #116209]	407	not determinedn.d.	+	+	+	+	+	-	+	+	+	+	+	+	+	+	-	+	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	+	-	+	+	+	-	-	-	-	+	+	-	-	-	-	-	-	-	+	+	-	-	+	-	-	-	-	+	+	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	+	+	-	+	+	-	-	-	-	-	+	-	+	+	-	+	+	+	+	-	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116209]	7525	-	+	+	+	-	-	-	-	-	-	-	-	+	+	-	+	+	-	-	-

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	threonine metabolism	100	10 of 10
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	pentose phosphate pathway	100	11 of 11
[Ref.: #66794]	taurine degradation	100	1 of 1
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	teichoic acid biosynthesis	100	1 of 1
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	sulfopterin metabolism	100	4 of 4
[Ref.: #66794]	lipoate biosynthesis	100	5 of 5
[Ref.: #66794]	ribulose monophosphate pathway	100	2 of 2
[Ref.: #66794]	ketogluconate metabolism	100	8 of 8
[Ref.: #66794]	aerobactin biosynthesis	100	1 of 1
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	palmitate biosynthesis	95.45	21 of 22
[Ref.: #66794]	tetrahydrofolate metabolism	92.86	13 of 14
[Ref.: #66794]	vitamin B1 metabolism	92.31	12 of 13
[Ref.: #66794]	Entner Doudoroff pathway	90	9 of 10
[Ref.: #66794]	serine metabolism	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	valine metabolism	88.89	8 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	C4 and CAM-carbon fixation	87.5	7 of 8
[Ref.: #66794]	propanol degradation	85.71	6 of 7
[Ref.: #66794]	reductive acetyl coenzyme A pathway	85.71	6 of 7
[Ref.: #66794]	photosynthesis	85.71	12 of 14
[Ref.: #66794]	methionine metabolism	84.62	22 of 26
[Ref.: #66794]	phenylalanine metabolism	84.62	11 of 13
[Ref.: #66794]	1,4-dihydroxy-6-naphthoate biosynthesis	83.33	5 of 6
[Ref.: #66794]	glutamate and glutamine metabolism	82.14	23 of 28
[Ref.: #66794]	glycogen metabolism	80	4 of 5
[Ref.: #66794]	propionate fermentation	80	8 of 10
[Ref.: #66794]	purine metabolism	78.72	74 of 94
[Ref.: #66794]	citric acid cycle	78.57	11 of 14
[Ref.: #66794]	heme metabolism	78.57	11 of 14
[Ref.: #66794]	CO2 fixation in Crenarchaeota	77.78	7 of 9
[Ref.: #66794]	allantoin degradation	77.78	7 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	77.78	7 of 9
[Ref.: #66794]	leucine metabolism	76.92	10 of 13
[Ref.: #66794]	pyrimidine metabolism	75.56	34 of 45
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	gluconeogenesis	75	6 of 8
[Ref.: #66794]	isoleucine metabolism	75	6 of 8
[Ref.: #66794]	butanoate fermentation	75	3 of 4
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	biotin biosynthesis	75	3 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	degradation of sugar alcohols	75	12 of 16
[Ref.: #66794]	peptidoglycan biosynthesis	73.33	11 of 15
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	vitamin B6 metabolism	72.73	8 of 11
[Ref.: #66794]	NAD metabolism	72.22	13 of 18
[Ref.: #66794]	ubiquinone biosynthesis	71.43	5 of 7
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	starch degradation	70	7 of 10
[Ref.: #66794]	alanine metabolism	68.97	20 of 29
[Ref.: #66794]	histidine metabolism	68.97	20 of 29
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	glycolate and glyoxylate degradation	66.67	4 of 6
[Ref.: #66794]	cyanate degradation	66.67	2 of 3
[Ref.: #66794]	d-mannose degradation	66.67	6 of 9
[Ref.: #66794]	sulfoquinovose degradation	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	arginine metabolism	66.67	16 of 24
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	acetyl CoA biosynthesis	66.67	2 of 3
[Ref.: #66794]	non-pathway related	65.79	25 of 38
[Ref.: #66794]	oxidative phosphorylation	63.74	58 of 91
[Ref.: #66794]	d-xylose degradation	63.64	7 of 11
[Ref.: #66794]	proline metabolism	63.64	7 of 11
[Ref.: #66794]	tryptophan metabolism	63.16	24 of 38
[Ref.: #66794]	isoprenoid biosynthesis	61.54	16 of 26
[Ref.: #66794]	urea cycle	61.54	8 of 13
[Ref.: #66794]	factor 420 biosynthesis	60	3 of 5
[Ref.: #66794]	glycine betaine biosynthesis	60	3 of 5
[Ref.: #66794]	cellulose degradation	60	3 of 5
[Ref.: #66794]	gallate degradation	60	3 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	myo-inositol biosynthesis	60	6 of 10
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	58.33	7 of 12
[Ref.: #66794]	lipid metabolism	58.06	18 of 31
[Ref.: #66794]	polyamine pathway	56.52	13 of 23
[Ref.: #66794]	degradation of sugar acids	56	14 of 25
[Ref.: #66794]	phenol degradation	55	11 of 20
[Ref.: #66794]	sulfate reduction	53.85	7 of 13
[Ref.: #66794]	kanosamine biosynthesis II	50	1 of 2
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	coenzyme A metabolism	50	2 of 4
[Ref.: #66794]	tyrosine metabolism	50	7 of 14
[Ref.: #66794]	degradation of pentoses	50	14 of 28
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	50	1 of 2
[Ref.: #66794]	cysteine metabolism	50	9 of 18
[Ref.: #66794]	glutathione metabolism	50	7 of 14
[Ref.: #66794]	lysine metabolism	47.62	20 of 42
[Ref.: #66794]	ascorbate metabolism	45.45	10 of 22
[Ref.: #66794]	metabolism of disaccharids	45.45	5 of 11
[Ref.: #66794]	nitrate assimilation	44.44	4 of 9
[Ref.: #66794]	degradation of hexoses	44.44	8 of 18
[Ref.: #66794]	cardiolipin biosynthesis	42.86	3 of 7
[Ref.: #66794]	creatinine degradation	40	2 of 5
[Ref.: #66794]	phenylacetate degradation (aerobic)	40	2 of 5
[Ref.: #66794]	phenylpropanoid biosynthesis	38.46	5 of 13
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	38.46	5 of 13
[Ref.: #66794]	dTDPLrhamnose biosynthesis	37.5	3 of 8
[Ref.: #66794]	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	coenzyme M biosynthesis	30	3 of 10
[Ref.: #66794]	3-phenylpropionate degradation	26.67	4 of 15
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	catecholamine biosynthesis	25	1 of 4
[Ref.: #66794]	carnitine metabolism	25	2 of 8
[Ref.: #66794]	bile acid biosynthesis, neutral pathway	23.53	4 of 17
[Ref.: #66794]	lipid A biosynthesis	22.22	2 of 9
[Ref.: #66794]	arachidonic acid metabolism	22.22	4 of 18
[Ref.: #66794]	4-hydroxymandelate degradation	22.22	2 of 9
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #226]	Sample type/isolated from	human faeces

[Ref.: #49264]	Sample type/isolated from	Human feces

[Ref.: #67770]	Sample type/isolated from	Human feces

[Ref.: #116209]	Sample type/isolated from	Human, Faeces
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X76436 (>99% sequence identity) for Alkalihalobacillus alcalophilus subclade from Microbeatlas

Aquatic Samples	25
Soil Samples	53
Animal Samples	80
Plant Samples	8
Total Samples	166

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #226]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116209]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	B.alcalophilus 16S ribosomal RNA	X60603	1430	ENA	1218173 tax ID	*
[Ref.: #20218]	B.alcalophilus (DSM 485 T) gene for 16S rRNA	X76436	1505	ENA	1218173 tax ID	*
[Ref.: #20218]	Bacillus alcalophilus gene for 16S rRNA, partial sequence	AB006919	274	ENA	1218173 tax ID	*
[Ref.: #20218]	Bacillus alcalophilus gene for 16S rRNA, partial sequence, strain: NBRC 15653	AB363730	1476	ENA	1218173 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Alkalihalobacillus alcalophilus ATCC 27647 = CGMCC 1.3604	GCA_000292245	contig	GenBank	1218173 tax ID
[Ref.: #67770]	Alkalihalobacillus alcalophilus ATCC 27647 = CGMCC 1.3604	GCA_004802515	scaffold	GenBank	1218173 tax ID
[Ref.: #66792]	Bacillus alcalophilus ATCC 27647	1218173.3	wgs	PATRIC	1218173 tax ID	*
[Ref.: #66792]	Bacillus alcalophilus ATCC 27647 = CGMCC 1.3604	1218173.9	wgs	PATRIC	1218173 tax ID	*
[Ref.: #66792]	Bacillus alcalophilus ATCC 27647	2519899572	draft	JGI IMG/M	1218173 tax ID	*

[Ref.: #226]	GC-content	37.0 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #226]	GC-content	36.7 mol%	Buoyant density centrifugation (BD)
[Ref.: #67770]	GC-content	37 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Alkalihalobacillus alcalophilus ATCC 27647 = CGMCC 1.3604 CGMCC 1.3604 NCBI: GCA_004802515.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus alcalophilus ATCC 27647 PATRIC: 1218173.3
[Ref.: #69480]	motile	yes	89.513	no
[Ref.: #69480]	gram-positive	yes	87.377	no
[Ref.: #69480]	anaerobic	no	98.93	no
[Ref.: #69480]	aerobic	yes	87.4	no
[Ref.: #69480]	halophile	yes	60.63	no
[Ref.: #69480]	spore-forming	yes	94.578	yes
[Ref.: #69480]	thermophile	no	98.603	no
[Ref.: #69480]	glucose-util	yes	91.325	no
[Ref.: #69480]	flagellated	yes	80.354	no
[Ref.: #69480]	glucose-ferment	no	91.681	no

Availability in culture collections External links

[Ref.: #226]

Culture collection no.

DSM 485, ATCC 27647, JCM 5262, NCIB 10436, NCIB 8772, NCTC 4553, CCUG 28516, NRRL B-14309, BCRC 15419, CECT 1, CGMCC 1.3604, CIP 103410, IAM 12461, IFO 15653, KCTC 1824, KCTC 3884, LMG 17938, LMG 7120, NBRC 15653, NCIMB 10436, NCIMB 8772, NRRL NRS-1548, VTT E-87298

[Ref.: #70249]

SI-ID 10532

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus suaedae sp. nov., isolated from the stem of Suaeda aralocaspica in north-west China.	Lei RF, Ma Q, Li YQ, Abudourousuli D, Wang S, Rouzi Z, Aosiman M, An DD, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.005362	2022	*
Phylogeny	Paralkalibacillus indicireducens gen., nov., sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor used for dyeing.	Hirota K, Nishita M, Matsuyama H, Yumoto I	Int J Syst Evol Microbiol	10.1099/ijsem.0.002248	2017	*
Phylogeny	Polygonibacillus indicireducens gen. nov., sp. nov., an indigo-reducing and obligate alkaliphile isolated from indigo fermentation liquor for dyeing.	Hirota K, Okamoto T, Matsuyama H, Yumoto I	Int J Syst Evol Microbiol	10.1099/ijsem.0.001405	2016	*
Phylogeny	Bacillus bogoriensis sp. nov., a novel alkaliphilic, halotolerant bacterium isolated from a Kenyan soda lake.	Vargas VA, Delgado OD, Hatti-Kaul R, Mattiasson B	Int J Syst Evol Microbiol	10.1099/ijs.0.63318-0	2005	*
Metabolism	The electrochemical proton potential of Bacillus alcalophilus.	Hoffmann A, Dimroth P	Eur J Biochem	10.1111/j.1432-1033.1991.tb16304.x	1991	*
Phylogeny	Taxonomy of alkaliphilic Bacillus strains.	Fritze D, Flossdorf J, Claus D	Int J Syst Bacteriol	10.1099/00207713-40-1-92	1990	*

References

#226	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 485

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23138

E. W. BOYER, M. B. INGLE, G. D. MERCER: Bacillus alcalophilus subsp. halodurans subsp. nov.: An Alkaline-Amylase-Producing, Alkalophilic Organism. IJSEM 23: 238 - 242 1973 ( DOI 10.1099/00207713-23-3-238 )

#34261 ; Curators of the CIP;
#49264 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 28516

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70249

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID10532.1 )

#116209 Collection of Institut Pasteur ; Curators of the CIP; CIP 103410
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Alkalihalobacillus alcalophilus in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Alkalihalobacillus alcalophilus ATCC 27647 = CGMCC 1.3604 CGMCC 1.3604

Bacillus alcalophilus ATCC 27647

Change proposal

Change proposal