Viridibacillus neidei (DSM 15031, JCM 11077, NRRL BD-87)

Name: Viridibacillus neidei (DSM 15031, JCM 11077, NRRL BD-87)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 569

Type strain

Culture col. no. DSM 15031 JCM 11077 NRRL BD-87 CIP 107432

NCBI tax ID(s) 101059

Special Collections DSMZ JCM Literature strains CIP

Links

Viridibacillus neidei DSM 15031 is an aerobe, spore-forming, mesophilic prokaryote that forms circular colonies and was isolated from soil.

spore-forming Gram-positive motile rod-shaped colony-forming aerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 100522

JCM 11077,NRRL BD-87,DSM 15031,KCTC 13924,CIP 107432,LMG 21635,LMG 22737

@ref 20215

Last LPSN update 2025-12-16 09:46:53

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Viridibacillus

Species Viridibacillus neidei

Full scientific name Viridibacillus neidei (Nakamura et al. 2002) Albert et al. 2007

Synonyms (1)

Bacillus neidei

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
23137	positive	3.0-5.0 µm	1.0 µm	rod-shaped
121257	positive			rod-shaped

Colony morphology

@ref	Colony size	Colony shape	Incubation period	Medium used
23137	1.0 mm	circular	1 day	TGY
23358				PCA, TSA and R2A
121257

Pigmentation

@ref	Production	Name
23137		no pigments

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5682	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
23358	PCA, TSA and R2A
23137	TGY
33794	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121257	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
5682	positive	growth	30	mesophilic
23137	positive	maximum	40-45	thermophilic
23137	positive	minimum	5-10	psychrophilic
23137	positive	optimum	28-30	mesophilic
33794	positive	growth	30	mesophilic
121257	positive	growth	10-37
121257	negative	growth	45
121257	negative	growth	55

Culture pH

@ref	Ability	Type	PH
23137	positive	growth	5-7
121257	positive	growth	6

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
23137	aerobe
121257	obligate aerobe

Spore formation

@ref	Type of spore	Spore formation
23358	endospore
121257

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23137	NaCl	positive	growth	5.0 %
121257	NaCl	positive	growth	0-10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23137	17256 ChEBI	2-deoxyadenosine	-	oxidation
23137	37054 ChEBI	3-hydroxybutyrate	+	oxidation
23137	16027 ChEBI	amp	-	oxidation
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
23137	15956 ChEBI	biotin	+	required for growth
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
23137	16947 ChEBI	citrate	-	oxidation
121257	16947 ChEBI	citrate	-	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	-	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
121257	4853 ChEBI	esculin	-	hydrolysis
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
23137	73784 ChEBI	glycyl-l-glutamate	-	oxidation
121257	606565 ChEBI	hippurate	+	hydrolysis
23137	16136 ChEBI	hydrogen sulfide	-	other
23137	17596 ChEBI	inosine	-	oxidation
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
23137	16977 ChEBI	L-alanine	-	oxidation
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
23137	16283 ChEBI	L-cystine	+	required for growth
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
23137	17632 ChEBI	nitrate	-	reduction
121257	17632 ChEBI	nitrate	-	reduction
121257	17632 ChEBI	nitrate	-	respiration
121257	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
23137	17272 ChEBI	propionate	-	oxidation
23137	15361 ChEBI	pyruvate	+	oxidation
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
23137	26948 ChEBI	thiamine	+	required for growth
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
23137	27897 ChEBI	tryptophan	-	energy source
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
23137	53423 ChEBI	tween 40	-	hydrolysis
23137	53426 ChEBI	tween 80	-	hydrolysis
23137	16695 ChEBI	uridine 5'-monophosphate	-	oxidation
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	ChEBI	Metabolite
23137	17698	chloramphenicol
23137	48923	erythromycin
23137	17076	streptomycin
23137	27902	tetracycline
23137	28864	tobramycin

Metabolite production

@ref	Chebi-ID	Metabolite	Production
23137	35581 ChEBI	indole
121257	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test	Indole test
121257	15688 ChEBI	acetoin	-
121257	17234 ChEBI	glucose		-
23137	35581 ChEBI	indole			-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
121257	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
121257	amylase	-
68382	beta-galactosidase	+	3.2.1.23	from API zym
121257	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121257	caseinase	-	3.4.21.50
23137	catalase	+	1.11.1.6
121257	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
121257	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
121257	gamma-glutamyltransferase	+	2.3.2.2
121257	gelatinase	+
121257	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
121257	lipase	-
68382	lipase (C 14)	-		from API zym
121257	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121257	ornithine decarboxylase	-	4.1.1.17
121257	oxidase	+
121257	protease	+
68382	trypsin	-	3.4.21.4	from API zym
23137	tryptophan deaminase	-	4.1.99.1
121257	tween esterase	-
121257	urease	+	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121257	-	+	+	+	-	+	+	-	-	+	+	+	-	+	-	-	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
121257	not determinedn.d.	-	-	-	-	+/-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
121257	not determinedn.d.	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type
5682	soil
121257	Environment, Soil

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5682	1	Risk group (German classification) According to TRBA 466
121257	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Bacillus sp. BD-87 16S ribosomal RNA gene, partial sequence	AF169520	1413		101059

GC content

@ref	GC-content (mol%)	Method
23358	35.0	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Proposal of Viridibacillus gen. nov. and reclassification of Bacillus arvi, Bacillus arenosi and Bacillus neidei as Viridibacillus arvi gen. nov., comb. nov., Viridibacillus arenosi comb. nov. and Viridibacillus neidei comb. nov.	Albert RA, Archambault J, Lempa M, Hurst B, Richardson C, Gruenloh S, Duran M, Worliczek HL, Huber BE, Rossello-Mora R, Schumann P, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.65256-0	2007
Phylogeny	Bacillus pycnus sp. nov. and Bacillus neidei sp. nov., round-spored bacteria from soil.	Nakamura LK, Shida O, Takagi H, Komagata K	Int J Syst Evol Microbiol	10.1099/00207713-52-2-501	2002

References

#5682	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15031
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#23137	L K Nakamura, O Shida, H Takagi, K Komagata: Bacillus pycnus sp. nov. and Bacillus neidei sp. nov., round-spored bacteria from soil.. IJSEM 52: 501 - 505 2002 ( DOI 10.1099/00207713-52-2-501 , PubMed 11931162 )
#23358	Richard A. Albert, Julieta Archambault, Melissa Lempa, Beth Hurst, Christine Richardson, Stephanie Gruenloh, Metin Duran, Hanna Lucia Worliczek, Birgit E. Huber, Ramon Rosselló-Mora, Peter Schumann, Hans-Jürgen Busse: Proposal of Viridibacillus gen. nov. and reclassification of Bacillus arvi, Bacillus arenosi and Bacillus neidei as Viridibacillus arvi gen. nov., comb. nov., Viridibacillus arenosi comb. nov. and Viridibacillus neidei comb. nov.. IJSEM 57: 2729 - 2737 2007 ( DOI 10.1099/ijs.0.65256-0 , PubMed 18048716 )
#33794	; Curators of the CIP;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#121257	Collection of Institut Pasteur ; Curators of the CIP; CIP 107432
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Viridibacillus neidei (DSM 15031, JCM 11077, NRRL BD-87)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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