Name: Chryseobacterium wanjuense R2A 10-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5682

Type strain:

Species: Chryseobacterium wanjuense

Strain Designation: R2A 10-2

Culture col. no.: DSM 17724, KACC 11468, CIP 110181

Strain history: CIP <- 2010, DSMZ <- S.W. Kwon, Nat. Inst. Agricul. Sci. Technol., Suwon, Korea: strain R2A 10-2 <- H.Y. Weon, KACC

NCBI tax ID(s): 356305 (species)

SI-ID 294440

DSM 17724, KACC 11468, CIP 110181

Section

Chryseobacterium wanjuense R2A 10-2 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from greenhouse soil cultivated with lettuce Lactuca sativa L..

Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Weeksellaceae*
	Genus	*Chryseobacterium*
	Species	**Chryseobacterium wanjuense**
	Full Scientific Name (LPSN)	Chryseobacterium wanjuense Weon et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #31750]	Gram stain	negative
[Ref.: #123905]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.993

[Ref.: #31750]	Cell length	2-3.5 µm

[Ref.: #31750]	Cell width	0.7-0.8 µm

[Ref.: #31750]	Cell shape	rod-shaped
[Ref.: #123905]	Cell shape	rod-shaped

[Ref.: #31750]	Motility	no
[Ref.: #123905]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %91.915

[Ref.: #31750]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6920]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #6920]

Culture medium growth

[Ref.: #6920]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6920]

Culture medium composition

expand / minimize

[Ref.: #39758]

Culture medium

MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromati

[Ref.: #39758]

Culture medium growth

[Ref.: #39758]

Culture medium composition

expand / minimize

[Ref.: #123905]

Culture medium

CIP Medium 566

[Ref.: #123905]

Culture medium growth

[Ref.: #123905]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #6920]	growth	28 °C
[Ref.: #31750]	growth	05-37 °C
[Ref.: #31750]	optimum	28 °C
[Ref.: #39758]	growth	30 °C

[Ref.: #6920]	Temperature range	mesophilic
[Ref.: #31750]	Temperature range	mesophilic
[Ref.: #39758]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31750]			optimum	7
[Ref.: #31750]			growth	05-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31750]	Oxygen tolerance	aerobe
[Ref.: #123905]	Oxygen tolerance	obligate aerobe

[Ref.: #31750]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.995

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31750]		NaCl		growth	0-2	%
[Ref.: #31750]		NaCl		optimum	0-1	%

[Ref.: #31750]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31750]	glucose ChEBI	+	carbon source
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31750]	glycogen ChEBI	+	carbon source
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31750]	malonate ChEBI	+	carbon source
[Ref.: #31750]	maltose ChEBI	+	carbon source
[Ref.: #31750]	mannose ChEBI	+	carbon source
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #123905]	nitrate ChEBI	-	reduction
[Ref.: #123905]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31750]	proline ChEBI	+	carbon source
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #123905]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31750]	catalase	+	1.11.1.6
[Ref.: #123905]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #31750]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #31750]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123905]	oxidase	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #123905]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #123905]	11718	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	+/-	+/-	+/-	-	-	-	-	-	-	-	-	-	+/-	-	+/-	-	-	+/-	-	-	-	+/-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123905]	12419	-	+	+	+	+	+	+	+	+	-	+	+	-	-	-	+	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6920]	Sample type/isolated from	greenhouse soil cultivated with lettuce Lactuca sativa L.
[Ref.: #6920]	Host species	Lactuca sativa
[Ref.: #6920]	Geographic location (country and/or sea, region)	Wanju Province
[Ref.: #6920]	Country	Republic of Korea
[Ref.: #6920]	Country ISO 3 Code	KOR
[Ref.: #6920]	Continent	Asia

[Ref.: #123905]	Sample type/isolated from	Environment, Greenhouse soil cultivated with lettuce, Lactua sativa
[Ref.: #123905]	Geographic location (country and/or sea, region)	Wanju
[Ref.: #123905]	Country	Republic of Korea
[Ref.: #123905]	Country ISO 3 Code	KOR
[Ref.: #123905]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Greenhouse
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ256729 (>99% sequence identity) for Chryseobacterium wanjuense subclade from Microbeatlas

Aquatic Samples	24
Soil Samples	27
Animal Samples	3
Plant Samples	253
Total Samples	307

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6920]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123905]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6920]	Chryseobacterium wanjuense strain R2A10-2 16S ribosomal RNA gene, partial sequence	DQ256729	1462	ENA	356305 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Chryseobacterium wanjuense DSM 17724	GCA_900111495	contig	GenBank	356305 tax ID	*
[Ref.: #66792]	Chryseobacterium wanjuense strain DSM 17724	356305.5	wgs	PATRIC	356305 tax ID	*
[Ref.: #66792]	Chryseobacterium wanjuense DSM 17724	2684622833	draft	JGI IMG/M	356305 tax ID	*

[Ref.: #6920]

GC-content

37.8 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Chryseobacterium wanjuense strain DSM 17724 PATRIC: 356305.5
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Chryseobacterium wanjuense strain DSM 17724 PATRIC: 356305.5
[Ref.: #69480]	gram-positive	no	98.183	yes
[Ref.: #69480]	anaerobic	no	99.603	yes
[Ref.: #69480]	halophile	no	95.175	yes
[Ref.: #69480]	spore-forming	no	96.136	yes
[Ref.: #69480]	glucose-util	yes	85.842	yes
[Ref.: #69480]	motile	no	92.794	no
[Ref.: #69480]	flagellated	no	97.033	yes
[Ref.: #69480]	thermophile	no	98.778	no
[Ref.: #69480]	aerobic	yes	90.83	yes
[Ref.: #69480]	glucose-ferment	no	92.774	no

Availability in culture collections External links

[Ref.: #6920]

Culture collection no.

DSM 17724, KACC 11468, CIP 110181

[Ref.: #75164]

SI-ID 294440

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Chryseobacterium wanjuense sp. nov., isolated from greenhouse soil in Korea.	Weon HY, Kim BY, Yoo SH, Kwon SW, Cho YH, Go SJ, Stackebrandt E	Int J Syst Evol Microbiol	10.1099/ijs.0.64179-0	2006	*

References

#6920

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17724

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31750

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28024 (see below)

#39758 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#75164

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID294440.1 )

#123905 Collection of Institut Pasteur ; Curators of the CIP; CIP 110181

#28024

IJSEM 1501 2006 ( DOI 10.1099/ijs.0.64179-0 , PubMed 16825619 )

* These data were automatically processed and therefore are not curated

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Chryseobacterium wanjuense strain DSM 17724

Chryseobacterium wanjuense strain DSM 17724

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