Name: Cellulophaga fucicola NN015860
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 5502

Type strain:

Species: Cellulophaga fucicola

Strain Designation: NN015860

Culture col. no.: DSM 24786, ATCC 700863, CIP 106308, JCM 21778, LMG 18536, NCIMB 13611, IAM 15330

Strain history: CIP <- 1999, P. Nielsen, Novo Nordisk, Denmark: strain NN015860

NCBI tax ID(s): 1121273 (strain), 76595 (species)

SI-ID 12968

LMG 18536, ATCC 700863, NCIMB 13611, CIP 106308, JCM 21778, IAM 15330, DSM 24786

Special Collections

Section

Cellulophaga fucicola NN015860 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from surface of brown alga Fucus serratus L..

Gram-negative
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Cellulophaga*
	Species	**Cellulophaga fucicola**
	Full Scientific Name (LPSN)	Cellulophaga fucicola Johansen et al. 1999

Information on morphological and physiological properties Morphology

[Ref.: #119478]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %98.444

[Ref.: #119478]	Cell shape	rod-shaped

[Ref.: #119478]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %92.5

[Ref.: #17772]

Incubation period

1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17772]

Culture medium

TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)

[Ref.: #17772]

Culture medium growth

[Ref.: #17772]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17772]

Culture medium composition

expand / minimize

[Ref.: #17772]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #17772]

Culture medium growth

[Ref.: #17772]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17772]

Culture medium composition

expand / minimize

[Ref.: #40288]

Culture medium

Marine agar (MA)

[Ref.: #40288]

Culture medium growth

[Ref.: #40288]

Culture medium composition

expand / minimize

[Ref.: #119478]

Culture medium

CIP Medium 326

[Ref.: #119478]

Culture medium growth

[Ref.: #119478]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #119478]

Culture medium

CIP Medium 13

[Ref.: #119478]

Culture medium growth

[Ref.: #119478]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #17772]		growth	28 °C
[Ref.: #40288]		growth	25 °C
[Ref.: #67770]		growth	25 °C
[Ref.: #119478]		growth	5-30 °C
[Ref.: #119478]	no	growth	37 °C
[Ref.: #119478]	no	growth	41 °C
[Ref.: #119478]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #119478]

Oxygen tolerance

obligate aerobe

[Ref.: #69480]	Ability of spore formation	no Confidence in %92.333
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Salt	Tested relation		Salt conc.
[Ref.: #119478]	NaCl		growth	2-4	%
[Ref.: #119478]	NaCl	no	growth	0	%
[Ref.: #119478]	NaCl	no	growth	6	%
[Ref.: #119478]	NaCl	no	growth	8	%
[Ref.: #119478]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #119478]	esculin ChEBI	-	hydrolysis
[Ref.: #119478]	hippurate ChEBI	-	hydrolysis
[Ref.: #119478]	malonate ChEBI	-	assimilation
[Ref.: #119478]	nitrate ChEBI	-	reduction
[Ref.: #119478]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #119478]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #119478]		acetoin ChEBI	+
[Ref.: #119478]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #119478]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #119478]	amylase	+
[Ref.: #119478]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119478]	caseinase	+	3.4.21.50
[Ref.: #119478]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #119478]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #119478]	gelatinase	-
[Ref.: #119478]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #119478]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119478]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119478]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119478]	oxidase	-
[Ref.: #119478]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #119478]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119478]	tryptophan deaminase	-
[Ref.: #119478]	tween esterase	-
[Ref.: #119478]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #119478]	1635	not determinedn.d.	+	-	+	+	+	-	+	+	-	+	+	+	-	-	-	+	+	+	+	-	-	+	+	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+	+	+	-	+	+	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119478]	9388	-	+	-	+	-	+	+	+	-	-	+	+	-	-	-	-	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17772]	Sample type/isolated from	surface of brown alga Fucus serratus L.
[Ref.: #17772]	Host species	Fucus serratus
[Ref.: #17772]	Geographic location (country and/or sea, region)	Kattegat
[Ref.: #17772]	Country	Denmark
[Ref.: #17772]	Country ISO 3 Code	DNK
[Ref.: #17772]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Surface of the brown alga (Fucus serratus) L. on the island of Hirsholm, offshore from Frederikshavn
[Ref.: #67770]	Host species	Fucus serratus
[Ref.: #67770]	Geographic location (country and/or sea, region)	Jutland
[Ref.: #67770]	Country	Denmark
[Ref.: #67770]	Country ISO 3 Code	DNK
[Ref.: #67770]	Continent	Europe

[Ref.: #119478]	Sample type/isolated from	Surface of brown alga Fucus serratus L.
[Ref.: #119478]	Geographic location (country and/or sea, region)	Kattegat
[Ref.: #119478]	Country	Denmark
[Ref.: #119478]	Country ISO 3 Code	DNK
[Ref.: #119478]	Continent	Europe
[Ref.: #119478]	Isolation date	1995
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Host	#Algae	#Brown Algae

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ005973 (>99% sequence identity) for Cellulophaga fucicola subclade from Microbeatlas

Aquatic Samples	350
Soil Samples	7
Animal Samples	19
Plant Samples
Total Samples	376

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17772]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119478]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17772]	Cytophaga fucicola 16S rRNA gene, type strain NN015860, partial	AJ005973	1472	GenBank ENA	76595 tax ID
[Ref.: #20218]	Cellulophaga fucicola 16S-23S ribosomal RNA intergenic spacer, partial sequence	EF667142	662	GenBank ENA	76595 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Cellulophaga fucicola DSM 24786	GCA_900119145	scaffold	GenBank ENA	76595 tax ID
[Ref.: #66792]	Cellulophaga fucicola strain DSM 24786	76595.6	wgs	PATRIC	76595 tax ID	*
[Ref.: #66792]	Cellulophaga fucicola DSM 24786	2595699004	draft	JGI IMG/M	76595 tax ID	*

[Ref.: #67770]

GC-content

32.4 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Cellulophaga fucicola DSM 24786 NCBI: GCA_900119145.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Cellulophaga fucicola strain DSM 24786 PATRIC: 76595.6
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	98.444	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.219	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	87.489	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	92.333	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	99.797	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	92.5	no

Availability in culture collections External links

[Ref.: #17772]

Culture collection no.

DSM 24786, ATCC 700863, CIP 106308, JCM 21778, LMG 18536, NCIMB 13611, IAM 15330

[Ref.: #74987]

SI-ID 12968

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Cellulophaga geojensis sp. nov., a member of the family Flavobacteriaceae isolated from marine sand.	Park S, Oh KH, Lee SY, Oh TK, Yoon JH	Int J Syst Evol Microbiol	10.1099/ijs.0.033340-0	2011	*
Phylogeny	Description of Cellulophaga baltica gen. nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of [Cytophaga] lytica to Cellulophaga lytica gen. nov., comb. nov.	Johansen JE, Nielsen P, Sjoholm C	Int J Syst Bacteriol	10.1099/00207713-49-3-1231	1999	*

References

#17772

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24786

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40288 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#74987

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID12968.1 )

#119478 Collection of Institut Pasteur ; Curators of the CIP; CIP 106308
* These data were automatically processed and therefore are not curated

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Cellulophaga fucicola DSM 24786

Cellulophaga fucicola strain DSM 24786

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