Name: Microbulbifer hydrolyticus IRE-31
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 465

Type strain:

Species: Microbulbifer hydrolyticus

Strain Designation: IRE-31

Culture col. no.: DSM 11525, ATCC 700072, IRE 31, CIP 105235

Strain history: CIP <- 1997, J. Gonzales, Georgia Univ., Athens, GA, USA: strain IRE-31

NCBI tax ID(s): 48074 (species)

SI-ID 43755

ATCC 700072, CIP 105235, DSM 11525, NCIMB 14044

Section

Microbulbifer hydrolyticus IRE-31 is a facultative aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from enrichment community growing on the high molecular weight fraction of a black liquor sample from the Federal Paper Board Company Inc..

colony-forming
Gram-negative
rod-shaped
facultative aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Cellvibrionales*
	Family	*Microbulbiferaceae*
	Genus	*Microbulbifer*
	Species	**Microbulbifer hydrolyticus**
	Full Scientific Name (LPSN)	Microbulbifer hydrolyticus González et al. 1997

Information on morphological and physiological properties Morphology

[Ref.: #23292]	Gram stain	negative
[Ref.: #120004]	Gram stain	negative

[Ref.: #23292]	Cell length	1.1-1.7 µm

[Ref.: #23292]	Cell width	0.3-0.5 µm

[Ref.: #23292]	Cell shape	rod-shaped
[Ref.: #120004]	Cell shape	oval-shaped

[Ref.: #23292]	Motility	no
[Ref.: #120004]	Motility	no

[Ref.: #23292]	Colony size	1 mm
[Ref.: #23292]	Colony color	cream
[Ref.: #23292]	Colony shape	circular
[Ref.: #23292]	Incubation period	7 days
[Ref.: #23292]	Cultivation medium used	Marine agar 2216

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4400]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #4400]

Culture medium growth

[Ref.: #4400]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4400]

Culture medium composition

expand / minimize

[Ref.: #36399]

Culture medium

Marine agar (MA)

[Ref.: #36399]

Culture medium growth

[Ref.: #36399]

Culture medium composition

expand / minimize

[Ref.: #120004]

Culture medium

CIP Medium 13

[Ref.: #120004]

Culture medium growth

[Ref.: #120004]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #4400]		growth	28-37 °C
[Ref.: #36399]		growth	25 °C
[Ref.: #120004]		growth	10-41 °C
[Ref.: #120004]	no	growth	5 °C

[Ref.: #4400]	Temperature range	mesophilic
[Ref.: #36399]	Temperature range	mesophilic
[Ref.: #120004]	Temperature range	psychrophilic

	pH	Kind of pH		pH
[Ref.: #23292]			optimum	7.5
[Ref.: #23292]			growth	6.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23292]	Oxygen tolerance	facultative aerobe
[Ref.: #120004]	Oxygen tolerance	obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #23292]	NaCl		growth	0.1-1	M
[Ref.: #23292]	NaCl		optimum	0.1-0.5	M
[Ref.: #120004]	NaCl		growth	2-6	%
[Ref.: #120004]	NaCl	no	growth	0	%
[Ref.: #120004]	NaCl	no	growth	8	%
[Ref.: #120004]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23292]	2-propanol ChEBI	-	growth
[Ref.: #23292]	3-hydroxybutyrate ChEBI	+	growth
[Ref.: #23292]	4-coumarate ChEBI	-	growth
[Ref.: #23292]	4-hydroxybenzoate ChEBI	-	growth
[Ref.: #23292]	acetate ChEBI	+	growth
[Ref.: #23292]	agar ChEBI	-	hydrolysis
[Ref.: #23292]	benzene ChEBI	-	growth
[Ref.: #23292]	benzoate ChEBI	-	growth
[Ref.: #23292]	butanol ChEBI	-	growth
[Ref.: #23292]	butyrate ChEBI	+	growth
[Ref.: #23292]	cellobiose ChEBI	+	growth
[Ref.: #23292]	cellulose ChEBI	+	hydrolysis
[Ref.: #23292]	chitin ChEBI	+	hydrolysis
[Ref.: #23292]	cinnamate ChEBI	-	growth
[Ref.: #23292]	citrate ChEBI	-	growth
[Ref.: #120004]	esculin ChEBI	-	hydrolysis
[Ref.: #23292]	ethanol ChEBI	-	growth
[Ref.: #23292]	ferulate ChEBI	+	assimilation
[Ref.: #23292]	ferulate ChEBI	+	growth
[Ref.: #23292]	formate ChEBI	-	growth
[Ref.: #23292]	fructose ChEBI	-	growth
[Ref.: #23292]	gelatin ChEBI	+	hydrolysis
[Ref.: #23292]	glucose ChEBI	-	builds acid from
[Ref.: #23292]	glucose ChEBI	+	growth
[Ref.: #23292]	glycerol ChEBI	-	growth
[Ref.: #23292]	glycine ChEBI	-	growth
[Ref.: #23292]	L-alanine ChEBI	+	growth
[Ref.: #23292]	L-arginine ChEBI	+	growth
[Ref.: #23292]	L-asparagine ChEBI	-	growth
[Ref.: #23292]	L-aspartate ChEBI	-	growth
[Ref.: #23292]	L-glutamate ChEBI	+	growth
[Ref.: #23292]	L-glutamine ChEBI	+	growth
[Ref.: #23292]	L-histidine ChEBI	+	growth
[Ref.: #23292]	L-isoleucine ChEBI	-	growth
[Ref.: #23292]	L-leucine ChEBI	+	growth
[Ref.: #23292]	L-lysine ChEBI	-	growth
[Ref.: #23292]	L-methionine ChEBI	-	growth
[Ref.: #23292]	L-phenylalanine ChEBI	-	growth
[Ref.: #23292]	L-proline ChEBI	+	growth
[Ref.: #23292]	L-serine ChEBI	+	growth
[Ref.: #23292]	L-threonine ChEBI	+	growth
[Ref.: #23292]	L-tryptophan ChEBI	-	growth
[Ref.: #23292]	L-valine ChEBI	-	growth
[Ref.: #23292]	lactose ChEBI	-	growth
[Ref.: #23292]	malate ChEBI	+	growth
[Ref.: #23292]	mannose ChEBI	-	growth
[Ref.: #23292]	methanol ChEBI	-	growth
[Ref.: #23292]	methylamine ChEBI	-	growth
[Ref.: #23292]	N-acetylglucosamine ChEBI	+	growth
[Ref.: #23292]	nitrate ChEBI	-	reduction
[Ref.: #120004]	nitrate ChEBI	-	reduction
[Ref.: #120004]	nitrate ChEBI	-	respiration
[Ref.: #120004]	nitrite ChEBI	-	reduction
[Ref.: #23292]	phenol ChEBI	-	growth
[Ref.: #23292]	propanol ChEBI	-	growth
[Ref.: #23292]	propionate ChEBI	+	growth
[Ref.: #23292]	pyruvate ChEBI	+	growth
[Ref.: #23292]	rhamnose ChEBI	-	growth
[Ref.: #23292]	salicylate ChEBI	-	growth
[Ref.: #23292]	starch ChEBI	+	hydrolysis
[Ref.: #23292]	succinate ChEBI	+	growth
[Ref.: #23292]	sucrose ChEBI	-	growth
[Ref.: #23292]	tween 80 ChEBI	+	hydrolysis
[Ref.: #23292]	vanillate ChEBI	+	assimilation
[Ref.: #23292]	vanillate ChEBI	+	growth
[Ref.: #23292]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #120004]	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes			no
[Ref.: #23292]	ampicillin ChEBI	yes	10	µg
[Ref.: #23292]	chloramphenicol ChEBI	yes	30	µg
[Ref.: #23292]	kanamycin ChEBI	yes	30	µg
[Ref.: #23292]	penicillin g ChEBI				yes	10	Unit
[Ref.: #23292]	streptomycin ChEBI				yes	10	µg
[Ref.: #23292]	tetracycline ChEBI	yes	30	µg
[Ref.: #23292]	vancomycin ChEBI				yes	30	µg

	Metabolite production	Metabolite	Production
[Ref.: #120004]		indole ChEBI	no
[Ref.: #23292]		polyhydroxybutyrate ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120004]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #23292]	amylase	+
[Ref.: #120004]	amylase	-
[Ref.: #120004]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120004]	caseinase	+	3.4.21.50
[Ref.: #23292]	catalase	+	1.11.1.6
[Ref.: #120004]	catalase	+	1.11.1.6
[Ref.: #23292]	cellulase	+	3.2.1.4
[Ref.: #23292]	chitinase	+	3.2.1.14
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #23292]	cytochrome oxidase	+	1.9.3.1
[Ref.: #120004]	DNase	+
[Ref.: #23292]	endo-1,4-beta-xylanase	+	3.2.1.8
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #23292]	gelatinase	+
[Ref.: #120004]	gelatinase	+
[Ref.: #120004]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #120004]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #23292]	lipase (Tween 80)	+
[Ref.: #120004]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120004]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120004]	oxidase	+
[Ref.: #120004]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120004]	tryptophan deaminase	-
[Ref.: #120004]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120004]	8550	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4400]	Sample type/isolated from	enrichment community growing on the high molecular weight fraction of a black liquor sample from the Federal Paper Board Company Inc.
[Ref.: #4400]	Geographic location (country and/or sea, region)	Georgia, Augusta
[Ref.: #4400]	Country	USA
[Ref.: #4400]	Country ISO 3 Code	USA
[Ref.: #4400]	Continent	North America

[Ref.: #120004]	Sample type/isolated from	Environment, Coastal seawater
[Ref.: #120004]	Geographic location (country and/or sea, region)	Georgia
[Ref.: #120004]	Country	United States of America
[Ref.: #120004]	Country ISO 3 Code	USA
[Ref.: #120004]	Continent	North America
[Ref.: #120004]	Isolation date	1994
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Industrial production
#Engineered	#Laboratory	#Lab enrichment
#Environmental	#Microbial community	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ608704 (>99% sequence identity) for Microbulbifer from Microbeatlas

Aquatic Samples	25
Soil Samples	5
Animal Samples
Plant Samples
Total Samples	30

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4400]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120004]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4400]	Microbulbifer hydrolyticus 16S ribosomal RNA gene, partial sequence	U58338	1393	ENA	48074 tax ID
[Ref.: #20218]	Microbulbifer hydrolyticus 16S rRNA gene, type strain DSM 11525T	AJ608704	1503	ENA	48074 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Microbulbifer hydrolyticus DSM 11525	GCA_014202835	contig	GenBank	48074 tax ID	*
[Ref.: #66792]	Microbulbifer hydrolyticus strain DSM 11525	48074.4	wgs	PATRIC	48074 tax ID	*
[Ref.: #66792]	Microbulbifer hydrolyticus DSM 11525	2861623431	draft	JGI IMG/M	48074 tax ID	*

[Ref.: #23292]

GC-content

57.7 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Microbulbifer hydrolyticus strain DSM 11525 PATRIC: 48074.4
[Ref.: #69480]	gram-positive	no	98.967	yes
[Ref.: #69480]	anaerobic	no	98.712	yes
[Ref.: #69480]	halophile	yes	73.016	no
[Ref.: #69480]	spore-forming	no	96.178	no
[Ref.: #69480]	glucose-util	yes	90.68	yes
[Ref.: #69480]	aerobic	yes	85.913	yes
[Ref.: #69480]	thermophile	no	98.849	yes
[Ref.: #69480]	motile	no	84.153	yes
[Ref.: #69480]	flagellated	no	88.274	yes
[Ref.: #69480]	glucose-ferment	no	88.688	no

Availability in culture collections External links

[Ref.: #4400]

Culture collection no.

DSM 11525, ATCC 700072, IRE 31, CIP 105235

[Ref.: #70144]

SI-ID 43755

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Effect of Different Carbon Sources on Cellulase Production by Marine Strain Microbulbifer hydrolyticus IRE-31-192.	Liu H, Zeng L, Jin Y, Nie K, Deng L, Wang F	Appl Biochem Biotechnol	10.1007/s12010-018-02948-1	2019	*
Phylogeny	Microbulbifer aestuariivivens sp. nov., isolated from a tidal flat.	Park S, Yoon SY, Ha MJ, Yoon JH	Int J Syst Evol Microbiol	10.1099/ijsem.0.001831	2017	*
Phylogeny	Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh.	Yoon JH, Kim IG, Shin DY, Kang KH, Park YH	Int J Syst Evol Microbiol	10.1099/ijs.0.02342-0	2003	*
Phylogeny	Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community.	Gonzalez JM, Mayer F, Moran MA, Hodson RE, Whitman WB	Int J Syst Bacteriol	10.1099/00207713-47-2-369	1997	*

References

#4400

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11525

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23292

J. M. GONZáLEZ, F. MAYER, M. A. MORAN, R. E. HODSON, W. B. WHITMAN: Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., Two Marine Bacteria from a Lignin-Rich Pulp Mill Waste Enrichment Community. IJSEM 47: 369 - 376 1997 ( DOI 10.1099/00207713-47-2-369 , PubMed 9103623 )

#36399 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#70144

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID43755.1 )

#120004 Collection of Institut Pasteur ; Curators of the CIP; CIP 105235
* These data were automatically processed and therefore are not curated

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Microbulbifer hydrolyticus strain DSM 11525

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