Name: Ignisphaera aggregans AQ1.S1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 4192

Type strain:

Species: Ignisphaera aggregans

Strain Designation: AQ1.S1, TG 866

Culture col. no.: DSM 17230, JCM 13409

Strain history: T. Niederberger AQ1.S1.

NCBI tax ID(s): 583356 (strain), 334771 (species)

SI-ID 290976

JCM 13409, DSM 17230

Section

Ignisphaera aggregans AQ1.S1 is an anaerobe, heterotroph, spore-forming archaeon that forms irregular colonies and was isolated from hot spring.

coccus-shaped
colony-forming
spore-forming
anaerobe
heterotroph
hyperthermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Thermoproteota*
	Class	*Thermoprotei*
	Order	*Desulfurococcales*
	Family	*Desulfurococcaceae*
	Genus	*Ignisphaera*
	Species	**Ignisphaera aggregans**
	Full Scientific Name (LPSN)	Ignisphaera aggregans Niederberger et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.97

[Ref.: #23221]	Cell length	1.0-1.5 µm

[Ref.: #23221]	Cell shape	coccus-shaped

[Ref.: #23221]

Colony shape

irregular

Multimedia content

[Ref.: #66793]

Caption:

electron microscopic image

[Ref.: #66793]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6827]

Culture medium

IGNISPHAERA MEDIUM (DSMZ Medium 1043)

[Ref.: #6827]

Culture medium growth

[Ref.: #6827]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6827]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #6827]	growth	92 °C
[Ref.: #23221]	growth	85-98 °C
[Ref.: #23221]	optimum	92-95 °C
[Ref.: #67770]	growth	92 °C

[Ref.: #6827]	Temperature range	hyperthermophilic
[Ref.: #23221]	Temperature range	hyperthermophilic
[Ref.: #67770]	Temperature range	hyperthermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %99.999

	pH	Kind of pH		pH
[Ref.: #23221]			optimum	6.4
[Ref.: #23221]			growth	5.4-7.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #6827]	Oxygen tolerance	anaerobe
[Ref.: #23221]	Oxygen tolerance	obligate anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.999

[Ref.: #23221]

Nutrition type

heterotroph

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.967

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23221]		NaCl		growth	0-0.5	%
[Ref.: #23221]		NaCl		optimum	0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23221]	beta-cyclodextrin ChEBI	+	carbon source
[Ref.: #23221]	elemental sulfur ChEBI	+	electron acceptor
[Ref.: #23221]	galactose ChEBI	+	carbon source
[Ref.: #23221]	glucomannan ChEBI	+	carbon source
[Ref.: #23221]	glucose ChEBI	+	carbon source
[Ref.: #23221]	glycogen ChEBI	+	carbon source
[Ref.: #23221]	lactose ChEBI	+	carbon source
[Ref.: #23221]	maltose ChEBI	+	carbon source
[Ref.: #23221]	mannose ChEBI	+	carbon source
[Ref.: #23221]	nitrate ChEBI	+	electron acceptor
[Ref.: #23221]	nitrite ChEBI	+	electron acceptor
[Ref.: #23221]	peptone	+	carbon source
[Ref.: #23221]	starch ChEBI	+	carbon source
[Ref.: #23221]	sulfite ChEBI	+	electron acceptor
[Ref.: #23221]	thioglycolate ChEBI	+	electron acceptor
[Ref.: #23221]	thiosulfate ChEBI	+	electron acceptor
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #23221]	chloramphenicol ChEBI	yes
[Ref.: #23221]	erythromycin ChEBI	yes
[Ref.: #23221]	novobiocin ChEBI		yes
[Ref.: #23221]	rifampicin ChEBI	yes
[Ref.: #23221]	streptomycin ChEBI		yes

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	cellulose degradation	100	5 of 5
[Ref.: #66794]	ribulose monophosphate pathway	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	valine metabolism	88.89	8 of 9
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	87.5	7 of 8
[Ref.: #66794]	C4 and CAM-carbon fixation	87.5	7 of 8
[Ref.: #66794]	photosynthesis	85.71	12 of 14
[Ref.: #66794]	threonine metabolism	80	8 of 10
[Ref.: #66794]	molybdenum cofactor biosynthesis	77.78	7 of 9
[Ref.: #66794]	palmitate biosynthesis	77.27	17 of 22
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	isoleucine metabolism	75	6 of 8
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	coenzyme A metabolism	75	3 of 4
[Ref.: #66794]	pentose phosphate pathway	72.73	8 of 11
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	starch degradation	70	7 of 10
[Ref.: #66794]	Entner Doudoroff pathway	70	7 of 10
[Ref.: #66794]	urea cycle	69.23	9 of 13
[Ref.: #66794]	vitamin B1 metabolism	69.23	9 of 13
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	NAD metabolism	66.67	12 of 18
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	d-mannose degradation	66.67	6 of 9
[Ref.: #66794]	methionine metabolism	65.38	17 of 26
[Ref.: #66794]	glutamate and glutamine metabolism	64.29	18 of 28
[Ref.: #66794]	purine metabolism	63.83	60 of 94
[Ref.: #66794]	gluconeogenesis	62.5	5 of 8
[Ref.: #66794]	leucine metabolism	61.54	8 of 13
[Ref.: #66794]	phenylalanine metabolism	61.54	8 of 13
[Ref.: #66794]	degradation of hexoses	61.11	11 of 18
[Ref.: #66794]	oxidative phosphorylation	60.44	55 of 91
[Ref.: #66794]	factor 420 biosynthesis	60	3 of 5
[Ref.: #66794]	glycogen metabolism	60	3 of 5
[Ref.: #66794]	myo-inositol biosynthesis	60	6 of 10
[Ref.: #66794]	pyrimidine metabolism	60	27 of 45
[Ref.: #66794]	propanol degradation	57.14	4 of 7
[Ref.: #66794]	degradation of sugar alcohols	56.25	9 of 16
[Ref.: #66794]	serine metabolism	55.56	5 of 9
[Ref.: #66794]	d-xylose degradation	54.55	6 of 11
[Ref.: #66794]	vitamin B6 metabolism	54.55	6 of 11
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	selenocysteine biosynthesis	50	3 of 6
[Ref.: #66794]	CMP-KDO biosynthesis	50	2 of 4
[Ref.: #66794]	cysteine metabolism	50	9 of 18
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	glycolate and glyoxylate degradation	50	3 of 6
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	tetrahydrofolate metabolism	50	7 of 14
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	50	1 of 2
[Ref.: #66794]	histidine metabolism	48.28	14 of 29
[Ref.: #66794]	sulfate reduction	46.15	6 of 13
[Ref.: #66794]	alanine metabolism	44.83	13 of 29
[Ref.: #66794]	CO2 fixation in Crenarchaeota	44.44	4 of 9
[Ref.: #66794]	polyamine pathway	43.48	10 of 23
[Ref.: #66794]	degradation of pentoses	42.86	12 of 28
[Ref.: #66794]	lysine metabolism	42.86	18 of 42
[Ref.: #66794]	tyrosine metabolism	42.86	6 of 14
[Ref.: #66794]	reductive acetyl coenzyme A pathway	42.86	3 of 7
[Ref.: #66794]	tryptophan metabolism	42.11	16 of 38
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
[Ref.: #66794]	flavin biosynthesis	40	6 of 15
[Ref.: #66794]	metabolism of amino sugars and derivatives	40	2 of 5
[Ref.: #66794]	hydrogen production	40	2 of 5
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	propionate fermentation	40	4 of 10
[Ref.: #66794]	lipid metabolism	38.71	12 of 31
[Ref.: #66794]	arginine metabolism	37.5	9 of 24
[Ref.: #66794]	ketogluconate metabolism	37.5	3 of 8
[Ref.: #66794]	non-pathway related	36.84	14 of 38
[Ref.: #66794]	proline metabolism	36.36	4 of 11
[Ref.: #66794]	degradation of sugar acids	36	9 of 25
[Ref.: #66794]	vitamin B12 metabolism	35.29	12 of 34
[Ref.: #66794]	isoprenoid biosynthesis	34.62	9 of 26
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	methanogenesis from CO2	33.33	4 of 12
[Ref.: #66794]	coenzyme M biosynthesis	30	3 of 10
[Ref.: #66794]	4-hydroxyphenylacetate degradation	30	3 of 10
[Ref.: #66794]	mevalonate metabolism	28.57	2 of 7
[Ref.: #66794]	citric acid cycle	28.57	4 of 14
[Ref.: #66794]	glutathione metabolism	28.57	4 of 14
[Ref.: #66794]	cardiolipin biosynthesis	28.57	2 of 7
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	metabolism of disaccharids	27.27	3 of 11
[Ref.: #66794]	peptidoglycan biosynthesis	26.67	4 of 15
[Ref.: #66794]	ppGpp biosynthesis	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	ascorbate metabolism	22.73	5 of 22
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6827]	Sample type/isolated from	hot spring
[Ref.: #6827]	Geographic location (country and/or sea, region)	Rotorua, Kuirau Park
[Ref.: #6827]	Country	New Zealand
[Ref.: #6827]	Country ISO 3 Code	NZL
[Ref.: #6827]	Continent	Australia and Oceania

[Ref.: #23221]	Sample type/isolated from	nearneutral, boiling spring in Kuirau Park

[Ref.: #67770]	Sample type/isolated from	Terrestrial, near-neutral hot spring in Kuirau Park
[Ref.: #67770]	Geographic location (country and/or sea, region)	Rotorua
[Ref.: #67770]	Country	New Zealand
[Ref.: #67770]	Country ISO 3 Code	NZL
[Ref.: #67770]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ060321 (>99% sequence identity) for Ignisphaera aggregans subclade from Microbeatlas

Aquatic Samples	26
Soil Samples
Animal Samples
Plant Samples
Total Samples	26

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6827]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6827]	Ignisphaera aggregans strain AQ1.S1 16S ribosomal RNA gene, partial sequence	DQ060321	1491	ENA	583356 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Ignisphaera aggregans DSM 17230	GCA_000145985	complete	GenBank	583356 tax ID	*
[Ref.: #66792]	Ignisphaera aggregans AQ1.S1, DSM 17230	648028035	complete	JGI IMG/M	583356 tax ID	*
[Ref.: #66792]	Ignisphaera aggregans DSM 17230	583356.9	complete	PATRIC	583356 tax ID	*

[Ref.: #6827]	GC-content	52.9 mol%
[Ref.: #23221]	GC-content	52.9 mol%	Thermal denaturation, fluorometry
[Ref.: #67770]	GC-content	52.9 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Ignisphaera aggregans DSM 17230 DSM 17230 NCBI: GCA_000145985.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Ignisphaera aggregans DSM 17230 DSM 17230 NCBI: GCA_000145985.1
[Ref.: #69480]	motile	no	82.501	no
[Ref.: #69480]	flagellated	no	94.033	no
[Ref.: #69480]	gram-positive	no	97.69	no
[Ref.: #69480]	anaerobic	yes	99.165	yes
[Ref.: #69480]	aerobic	no	97.893	yes
[Ref.: #69480]	halophile	no	85.959	no
[Ref.: #69480]	spore-forming	no	97.316	no
[Ref.: #69480]	thermophile	yes	100	no
[Ref.: #69480]	glucose-util	yes	87.188	yes
[Ref.: #69480]	glucose-ferment	no	50	no

Availability in culture collections External links

[Ref.: #6827]

Culture collection no.

DSM 17230, JCM 13409

[Ref.: #73692]

SI-ID 290976

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete genome sequence of Ignisphaera aggregans type strain (AQ1.S1).	Goker M, Held B, Lapidus A, Nolan M, Spring S, Yasawong M, Lucas S, Glavina Del Rio T, Tice H, Cheng JF, Goodwin L, Tapia R, Pitluck S, Liolios K, Ivanova N, Mavromatis K, Mikhailova N, Pati A, Chen A, Palaniappan K, Brambilla E, Land M, Hauser L, Chang YJ, Jeffries CD, Brettin T, Detter JC, Han C, Rohde M, Sikorski J, Woyke T, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP	Stand Genomic Sci	10.4056/sigs.1072907	2010	*
Phylogeny	The microbial ecology of a high-temperature near-neutral spring situated in Rotorua, New Zealand.	Niederberger TD, Ronimus RS, Morgan HW	Microbiol Res	10.1016/j.micres.2006.09.001	2007	*
Phylogeny	Ignisphaera aggregans gen. nov., sp. nov., a novel hyperthermophilic crenarchaeote isolated from hot springs in Rotorua and Tokaanu, New Zealand.	Niederberger TD, Gotz DK, McDonald IR, Ronimus RS, Morgan HW	Int J Syst Evol Microbiol	10.1099/ijs.0.63899-0	2006	*

References

#6827

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17230

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23221

Thomas D. Niederberger, Dorothee K. Götz, Ian R. McDonald, Ron S. Ronimus, Hugh W. Morgan: Ignisphaera aggregans gen. nov., sp. nov., a novel hyperthermophilic crenarchaeote isolated from hot springs in Rotorua and Tokaanu, New Zealand. IJSEM 56: 965 - 971 2006 ( DOI 10.1099/ijs.0.63899-0 , PubMed 16627639 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#73692

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID290976.1 )

* These data were automatically processed and therefore are not curated

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Ignisphaera aggregans DSM 17230 DSM 17230

Ignisphaera aggregans DSM 17230 DSM 17230

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