Desulfonatronum cooperativum (DSM 16749, VKM B-2329, Z-7999)

Name: Desulfonatronum cooperativum (DSM 16749, VKM B-2329, Z-7999)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 4065

Type strain

Strain Designation Z-7999

Culture col. no. DSM 16749 VKM B-2329 Z-7999

NCBI tax ID(s) 292807

Special Collections DSMZ Literature strains

Links

Desulfonatronum cooperativum Z-7999 is an anaerobe, lithotroph, mesophilic prokaryote that was isolated from deposits of soda lake Khadin.

Gram-negative motile vibrio-shaped anaerobe lithotroph mesophilic 16S sequence

Name and taxonomic classification

SI-ID 138977

DSM 16749,VKM B-2329

@ref 20215

Last LPSN update 2025-12-16 09:47:43

Domain Pseudomonadati

Phylum Thermodesulfobacteriota

Class Desulfovibrionia

Order Desulfovibrionales

Family Desulfonatronaceae

Genus Desulfonatronum

Species Desulfonatronum cooperativum

Full scientific name Desulfonatronum cooperativum Zhilina et al. 2005

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
23210	negative	1.0-2.5 µm	0.4-0.5 µm	vibrio-shaped		polar

Colony morphology

6492

Incubation period3-7 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6492	DESULFONATRONUM MEDIUM (DSMZ Medium 813)		Medium recipe at MediaDive	Name: DESULFONATRONUM MEDIUM (DSMZ Medium 813) Composition: NaCl 9.98004 g/l Na2SO4 4.99002 g/l Na-formate 3.99202 g/l Na2CO3 3.49301 g/l Yeast extract 0.998004 g/l NH4Cl 0.998004 g/l Na2S x 9 H2O 0.499002 g/l KCl 0.199601 g/l KH2PO4 0.199601 g/l MgCl2 x 6 H2O 0.0998004 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l CoCl2 x 6 H2O 0.000189621 g/l MnCl2 x 4 H2O 9.98004e-05 g/l Pyridoxine hydrochloride 9.98004e-05 g/l ZnCl2 6.98603e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l Folic acid 1.99601e-05 g/l Biotin 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6492	positive	growth	35	mesophilic
23210	positive	growth	15-40
23210	positive	optimum	35-38	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
23210	positive	optimum	8.0-9.0
23210	positive	growth	6.7-10.3	alkaliphile

Physiology and metabolism

Oxygen tolerance

6492

Oxygen toleranceanaerobe

Nutrition type

23210

Typelithotroph

Spore formation

23210

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23210	NaCl	positive	growth	>10-80 g/L
23210	NaCl	positive	optimum	5-15 g/L

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23210	30089 ChEBI	acetate	-	growth
23210	30089 ChEBI	acetate	+	carbon source
23210	35391 ChEBI	aspartate	-	growth
23210	17750 ChEBI	betaine	-	growth
23210	17968 ChEBI	butyrate	-	growth
23210		casamino acids	-	growth
23210	15354 ChEBI	choline	-	growth
23210	42160 ChEBI	dithionite	-	electron acceptor
23210	33403 ChEBI	elemental sulfur	-	electron acceptor
23210	16236 ChEBI	ethanol	-	growth
23210	15740 ChEBI	formate	+	electron donor
23210	29806 ChEBI	fumarate	-	electron acceptor
23210	29806 ChEBI	fumarate	-	growth
23210	29987 ChEBI	glutamate	-	growth
23210	24996 ChEBI	lactate	+	electron donor
23210	25115 ChEBI	malate	-	growth
23210	15792 ChEBI	malonate	-	growth
23210	17790 ChEBI	methanol	-	growth
23210	17632 ChEBI	nitrate	-	electron acceptor
23210	16301 ChEBI	nitrite	-	electron acceptor
23210	30623 ChEBI	oxalate	-	growth
23210	17272 ChEBI	propionate	-	growth
23210	15361 ChEBI	pyruvate	-	growth
23210	30031 ChEBI	succinate	-	growth
23210	16189 ChEBI	sulfate	+	electron acceptor
23210	16189 ChEBI	sulfate	+	reduction
23210	17359 ChEBI	sulfite	+	reduction
23210	16094 ChEBI	thiosulfate	+	reduction
23210		yeast extract	-	growth

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Laboratory	#Lab enrichment
#Environmental	#Aquatic	#Lake (large)
#Condition	#Alkaline	-
#Condition	#Anoxic (anaerobic)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6492	deposits of soda lake Khadin	Tuva	Russia	RUS	Asia
23210	syntrophic acetate- decomposing culture enriched from anaerobic deposits of soda lake

Taxonmaps

69479

Global distribution of 16S sequence AY725424 (>99% sequence identity) for Desulfonatronum cooperativum subclade from Microbeatlas

Aquatic Samples	152
Soil Samples	11
Animal Samples	11
Plant Samples	2
Total Samples	176

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6492	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6492	Desulfonatronum cooperativum strain Z-7999 16S ribosomal RNA gene, partial sequence	AY725424	1493		292807

GC content

@ref	GC-content (mol%)	Method
6492	56.5
23210	55.5-57.5	Thermal denaturation, fluorometry

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genomic Insights into Syntrophic Lifestyle of 'Candidatus Contubernalis alkaliaceticus' Based on the Reversed Wood-Ljungdahl Pathway and Mechanism of Direct Electron Transfer.	Frolov EN, Gavrilov SN, Toshchakov SV, Zavarzina DG.	Life (Basel)	10.3390/life13102084	2023
Metabolism	Sulfate-dependent acetate oxidation under extremely natron-alkaline conditions by syntrophic associations from hypersaline soda lakes.	Sorokin DY, Abbas B, Tourova TP, Bumazhkin BK, Kolganova TV, Muyzer G.	Microbiology (Reading)	10.1099/mic.0.075093-0	2014
	Microbial taxa related to natural hydrogen and methane emissions in serpentinite-hosted hyperalkaline springs of New Caledonia.	Quemeneur M, Mei N, Monnin C, Postec A, Guasco S, Jeanpert J, Maurizot P, Pelletier B, Erauso G.	Front Microbiol	10.3389/fmicb.2023.1196516	2023
Metabolism	[Alkaliphilic sulfidogenesis on cellulose by combined cultures].	Zavarzin GA, Zhilina TN, Dulov LE.	Mikrobiologiia	10.1134/s0026261708040061	2008
Metabolism	["Candidatus contubernalis alkalaceticum," an obligately syntrophic alkaliphilic bacterium capable of anaerobic acetate oxidation in a coculture with Desulfonatronum cooperativum].	Zhilina TN, Zavarzina DG, Kolganova TV, Turova TP, Zavarzin GA.	Mikrobiologiia	10.1007/s11021-005-0126-4	2005
Metabolism	Syntrophic growth of alkaliphilic anaerobes controlled by ferric and ferrous minerals transformation coupled to acetogenesis.	Zavarzina DG, Gavrilov SN, Chistyakova NI, Antonova AV, Gracheva MA, Merkel AY, Perevalova AA, Chernov MS, Zhilina TN, Bychkov AY, Bonch-Osmolovskaya EA.	ISME J	10.1038/s41396-019-0527-4	2020
Phylogeny	Temperature-dependent variations in sulfate-reducing communities associated with a terrestrial hydrocarbon seep.	Cheng TW, Lin LH, Lin YT, Song SR, Wang PL.	Microbes Environ	10.1264/jsme2.me14086	2014
Enzymology	Desulfonatronobacter acetoxydans sp. nov.,: a first acetate-oxidizing, extremely salt-tolerant alkaliphilic SRB from a hypersaline soda lake.	Sorokin DY, Chernyh NA, Poroshina MN.	Extremophiles	10.1007/s00792-015-0765-y	2015
Metabolism	Propionate and butyrate dependent bacterial sulfate reduction at extremely haloalkaline conditions and description of Desulfobotulus alkaliphilus sp. nov.	Sorokin DY, Detkova EN, Muyzer G.	Extremophiles	10.1007/s00792-009-0288-5	2010
Metabolism	Desulfohalophilus alkaliarsenatis gen. nov., sp. nov., an extremely halophilic sulfate- and arsenate-respiring bacterium from Searles Lake, California.	Blum JS, Kulp TR, Han S, Lanoil B, Saltikov CW, Stolz JF, Miller LG, Oremland RS.	Extremophiles	10.1007/s00792-012-0468-6	2012
Phylogeny	Desulfonatronum cooperativum sp. nov., a novel hydrogenotrophic, alkaliphilic, sulfate-reducing bacterium, from a syntrophic culture growing on acetate.	Zhilina TN, Zavarzina DG, Kuever J, Lysenko AM, Zavarzin GA	Int J Syst Evol Microbiol	10.1099/ijs.0.63490-0	2005

References

#6492	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16749
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23210	Tatjana N. Zhilina, Daria G. Zavarzina, Jan Kuever, Anatoly M. Lysenko, Georgy A. Zavarzin: Desulfonatronum cooperativum sp. nov., a novel hydrogenotrophic, alkaliphilic, sulfate-reducing bacterium, from a syntrophic culture growing on acetate. IJSEM 55: 1001 - 1006 2005 ( DOI 10.1099/ijs.0.63490-0 , PubMed 15879225 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Desulfonatronum cooperativum (DSM 16749, VKM B-2329, Z-7999)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Metabolite utilization

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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