Desulfobacula toluolica (DSM 7467, Tol 2)

Name: Desulfobacula toluolica (DSM 7467, Tol 2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 3979

Type strain

Culture col. no. DSM 7467 Tol 2

NCBI tax ID(s) 651182 28223

Special Collections DSMZ

Links

Desulfobacula toluolica DSM 7467 is an anaerobe, mesophilic prokaryote that was isolated from marine mud.

anaerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 48446

DSM 7467

@ref 20215

Last LPSN update 2025-12-16 09:47:42

Domain Pseudomonadati

Phylum Thermodesulfobacteriota

Class Desulfobacteria

Order Desulfobacterales

Family Desulfobacteraceae

Genus Desulfobacula

Species Desulfobacula toluolica

Full scientific name Desulfobacula toluolica Rabus et al. 2000

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
3172	DESULFOBACTERIUM MEDIUM (DSMZ Medium 383)		Medium recipe at MediaDive	Name: DESULFOBACTERIUM MEDIUM (DSMZ Medium 383; with strain-specific modifications) Composition: NaCl 20.9372 g/l MgCl2 x 6 H2O 2.99103 g/l Na2SO4 2.99103 g/l Na2CO3 0.997009 g/l KCl 0.498504 g/l Na2S x 9 H2O 0.398804 g/l Na-benzoate 0.398804 g/l NH4Cl 0.299103 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149551 g/l Yeast extract 0.0997009 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Riboflavin 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
3172	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

3172

Oxygen toleranceanaerobe

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	adipate degradation	100	2 of 2
66794	1,4-dihydroxy-6-naphthoate biosynthesis	100	6 of 6
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	CMP-KDO biosynthesis	100	4 of 4
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	palmitate biosynthesis	100	22 of 22
66794	methylglyoxal degradation	100	5 of 5
66794	valine metabolism	100	9 of 9
66794	biotin biosynthesis	100	4 of 4
66794	propanol degradation	100	7 of 7
66794	propionate fermentation	100	10 of 10
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	folate polyglutamylation	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	sulfopterin metabolism	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	toluene degradation	100	4 of 4
66794	vitamin B1 metabolism	92.31	12 of 13
66794	lipid A biosynthesis	88.89	8 of 9
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	phenylalanine metabolism	84.62	11 of 13
66794	leucine metabolism	84.62	11 of 13
66794	NAD metabolism	83.33	15 of 18
66794	alanine metabolism	82.76	24 of 29
66794	threonine metabolism	80	8 of 10
66794	glycine betaine biosynthesis	80	4 of 5
66794	hydrogen production	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	glutamate and glutamine metabolism	78.57	22 of 28
66794	heme metabolism	78.57	11 of 14
66794	d-mannose degradation	77.78	7 of 9
66794	nitrate assimilation	77.78	7 of 9
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	glycolysis	76.47	13 of 17
66794	vitamin B12 metabolism	76.47	26 of 34
66794	ppGpp biosynthesis	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	acetate fermentation	75	3 of 4
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	purine metabolism	74.47	70 of 94
66794	flavin biosynthesis	73.33	11 of 15
66794	vitamin B6 metabolism	72.73	8 of 11
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	photosynthesis	71.43	10 of 14
66794	benzoyl-CoA degradation	71.43	5 of 7
66794	citric acid cycle	71.43	10 of 14
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	pyrimidine metabolism	71.11	32 of 45
66794	phosphatidylethanolamine bioynthesis	69.23	9 of 13
66794	acetoin degradation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	serine metabolism	66.67	6 of 9
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	enterobactin biosynthesis	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	isoprenoid biosynthesis	65.38	17 of 26
66794	proline metabolism	63.64	7 of 11
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	gluconeogenesis	62.5	5 of 8
66794	methionine metabolism	61.54	16 of 26
66794	lipid metabolism	61.29	19 of 31
66794	phenylacetate degradation (aerobic)	60	3 of 5
66794	factor 420 biosynthesis	60	3 of 5
66794	Entner Doudoroff pathway	60	6 of 10
66794	histidine metabolism	58.62	17 of 29
66794	glutathione metabolism	57.14	8 of 14
66794	lysine metabolism	57.14	24 of 42
66794	polyamine pathway	56.52	13 of 23
66794	tryptophan metabolism	55.26	21 of 38
66794	non-pathway related	55.26	21 of 38
66794	arginine metabolism	54.17	13 of 24
66794	sulfate reduction	53.85	7 of 13
66794	urea cycle	53.85	7 of 13
66794	oxidative phosphorylation	52.75	48 of 91
66794	denitrification	50	1 of 2
66794	myo-inositol biosynthesis	50	5 of 10
66794	degradation of sugar alcohols	50	8 of 16
66794	lactate fermentation	50	2 of 4
66794	alginate biosynthesis	50	2 of 4
66794	phenylmercury acetate degradation	50	1 of 2
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	sphingosine metabolism	50	3 of 6
66794	butanoate fermentation	50	2 of 4
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	tyrosine metabolism	50	7 of 14
66794	4-hydroxyphenylacetate degradation	50	5 of 10
66794	cysteine metabolism	44.44	8 of 18
66794	daunorubicin biosynthesis	44.44	4 of 9
66794	chlorophyll metabolism	44.44	8 of 18
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	lipoate biosynthesis	40	2 of 5
66794	coenzyme M biosynthesis	40	4 of 10
66794	glycogen metabolism	40	2 of 5
66794	vitamin K metabolism	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	ketogluconate metabolism	37.5	3 of 8
66794	metabolism of disaccharids	36.36	4 of 11
66794	phenol degradation	35	7 of 20
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	degradation of hexoses	33.33	6 of 18
66794	glycolate and glyoxylate degradation	33.33	2 of 6
66794	IAA biosynthesis	33.33	1 of 3
66794	pantothenate biosynthesis	33.33	2 of 6
66794	methanogenesis from CO2	33.33	4 of 12
66794	bile acid biosynthesis, neutral pathway	29.41	5 of 17
66794	aclacinomycin biosynthesis	28.57	2 of 7
66794	mevalonate metabolism	28.57	2 of 7
66794	degradation of sugar acids	28	7 of 25
66794	ascorbate metabolism	27.27	6 of 22
66794	pentose phosphate pathway	27.27	3 of 11
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	cyclohexanol degradation	25	1 of 4
66794	dTDPLrhamnose biosynthesis	25	2 of 8
66794	vitamin E metabolism	25	1 of 4
66794	degradation of pentoses	25	7 of 28
66794	catecholamine biosynthesis	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	phenylpropanoid biosynthesis	23.08	3 of 13
66794	4-hydroxymandelate degradation	22.22	2 of 9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Terrestrial	#Mud (Sludge)
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
3172	marine mud	Massachusetts, Woods Hole	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence AJ441316 (>99% sequence identity) for Desulfobacula toluolica from Microbeatlas

Aquatic Samples	489
Soil Samples	15
Animal Samples	1
Plant Samples	4
Total Samples	509

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3172	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
3172	Desulfobacula toluolica 16S rRNA gene, strain DSM 7467	AJ441316	1518		651182

GC content

3172

GC-content (mol%)42.0

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	From Structure to Function: Analysis of the First Monomeric Pyridoxal-5'-Phosphate-Dependent Transaminase from the Bacterium Desulfobacula toluolica.	Bakunova AK, Matyuta IO, Nikolaeva AY, Rakitina TV, Boyko KM, Popov VO, Bezsudnova EY.	Biomolecules	10.3390/biom14121591	2024
Genetics	Integrated Multi-omics Investigations Reveal the Key Role of Synergistic Microbial Networks in Removing Plasticizer Di-(2-Ethylhexyl) Phthalate from Estuarine Sediments.	Wei ST, Chen YL, Wu YW, Wu TY, Lai YL, Wang PH, Ismail W, Lee TH, Chiang YR.	mSystems	10.1128/msystems.00358-21	2021
Metabolism	Structural and Functional Characterization of an Electron Transfer Flavoprotein Involved in Toluene Degradation in Strictly Anaerobic Bacteria.	Vogt MS, Schuhle K, Kolzer S, Peschke P, Chowdhury NP, Kleinsorge D, Buckel W, Essen LO, Heider J.	J Bacteriol	10.1128/jb.00326-19	2019
Metabolism	Demethylation of dimethylsulfoniopropionate to 3-S-methylmercaptopropionate by marine sulfate-reducing bacteria.	van der Maarel MJ, Jansen M, Haanstra R, Meijer WG, Hansen TA.	Appl Environ Microbiol	10.1128/aem.62.11.3978-3984.1996	1996
Metabolism	Degradative capacities and 16S rRNA-targeted whole-cell hybridization of sulfate-reducing bacteria in an anaerobic enrichment culture utilizing alkylbenzenes from crude oil.	Rabus R, Fukui M, Wilkes H, Widdle F.	Appl Environ Microbiol	10.1128/aem.62.10.3605-3613.1996	1996
Enzymology	Analysis of membrane-protein complexes of the marine sulfate reducer Desulfobacula toluolica Tol2 by 1D blue native-PAGE complexome profiling and 2D blue native-/SDS-PAGE.	Wohlbrand L, Ruppersberg HS, Feenders C, Blasius B, Braun HP, Rabus R	Proteomics	10.1002/pmic.201500360	2016
Metabolism	Genetic evidence that the degradation of para-cresol by Geobacter metallireducens is catalyzed by the periplasmic para-cresol methylhydroxylase.	Chaurasia AK, Tremblay PL, Holmes DE, Zhang T	FEMS Microbiol Lett	10.1093/femsle/fnv145	2015
Metabolism	Fifteen years of physiological proteo(geno)mics with (marine) environmental bacteria.	Rabus R	Arch Physiol Biochem	10.3109/13813455.2014.951658	2014
Genetics	Draft genome sequence of an aromatic compound-degrading bacterium, Desulfobacula sp. TS, belonging to the Deltaproteobacteria.	Kim SJ, Park SJ, Jung MY, Kim JG, Min UG, Hong HJ, Rhee SK	FEMS Microbiol Lett	10.1111/1574-6968.12591	2014
Proteome	Complete genome, catabolic sub-proteomes and key-metabolites of Desulfobacula toluolica Tol2, a marine, aromatic compound-degrading, sulfate-reducing bacterium.	Wohlbrand L, Jacob JH, Kube M, Mussmann M, Jarling R, Beck A, Amann R, Wilkes H, Reinhardt R, Rabus R	Environ Microbiol	10.1111/j.1462-2920.2012.02885.x	2012
Metabolism	Population profiles of a stable, commensalistic bacterial culture grown with toluene under sulphate-reducing conditions.	Vogt C, Losche A, Kleinsteuber S, Muller S	Cytometry A	10.1002/cyto.a.20158	2005
Metabolism	Anaerobic degradation and transformation of p-toluidine by the sulfate-reducing bacterium Desulfobacula toluolica.	Raber T, Gorontzy T, Kleinschmidt M, Steinbach K, Blotevogel KH	Curr Microbiol	10.1007/s002849900359	1998
Metabolism	Conversion studies with substrate analogues of toluene in a sulfate-reducing bacterium, strain Tol2.	Rabus R, Widdel F	Arch Microbiol	10.1007/BF02529744	1995
Metabolism	Complete oxidation of toluene under strictly anoxic conditions by a new sulfate-reducing bacterium.	Rabus R, Nordhaus R, Ludwig W, Widdel F	Appl Environ Microbiol	10.1128/aem.59.5.1444-1451.1993	1993
Enzymology	A Novel Coenzyme A Analogue in the Anaerobic, Sulfate-Reducing, Marine Bacterium Desulfobacula toluolica Tol2(T).	Bruns S, Cakic N, Mitschke N, Kopke BJ, Rabus R, Wilkes H	Chembiochem	10.1002/cbic.202200584	2022
Phylogeny	Desulfoconvexum algidum gen. nov., sp. nov., a psychrophilic sulfate-reducing bacterium isolated from a permanently cold marine sediment.	Konneke M, Kuever J, Galushko A, Jorgensen BB	Int J Syst Evol Microbiol	10.1099/ijs.0.043703-0	2012

References

#3172	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7467
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Desulfobacula toluolica (DSM 7467, Tol 2)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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