Name: Simplicispira limi EMB 325
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 2999

Type strain:

Species: Simplicispira limi

Strain Designation: EMB 325

Culture col. no.: DSM 17964, KCTC 12608

Strain history: <- CO Jeon, Gyungsang Univ.

NCBI tax ID(s): 370980 (species)

SI-ID 399655

DSM 17964

Special Collections

Other strains from Simplicispira limi

Section

Simplicispira limi EMB 325 is an aerobe, Gram-negative, motile bacterium that was isolated from activated sludge.

Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Comamonadaceae*
	Genus	*Simplicispira*
	Species	**Simplicispira limi**
	Full Scientific Name (LPSN)	Simplicispira limi Lu et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #31869]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative

[Ref.: #31869]	Cell length	1.6 µm

[Ref.: #31869]	Cell width	0.75 µm

[Ref.: #31869]	Cell shape	rod-shaped

[Ref.: #31869]	Motility	yes

[Ref.: #31869]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7296]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #7296]

Culture medium growth

[Ref.: #7296]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7296]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #7296]	growth	25 °C
[Ref.: #31869]	growth	10-40 °C
[Ref.: #31869]	optimum	30 °C
[Ref.: #67771]	growth	25 °C

	pH	Kind of pH		pH
[Ref.: #31869]			optimum	07-08
[Ref.: #31869]			growth	06-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31869]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #67771]

Observation

quinones: Q-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31869]	2,3-butanediol ChEBI	+	carbon source
[Ref.: #31869]	2-oxopentanoate ChEBI	+	carbon source
[Ref.: #31869]	esculin ChEBI	+	hydrolysis
[Ref.: #31869]	fructose ChEBI	+	carbon source
[Ref.: #31869]	lactate ChEBI	+	carbon source
[Ref.: #31869]	lactose ChEBI	+	carbon source
[Ref.: #31869]	mannitol ChEBI	+	carbon source
[Ref.: #31869]	methyl pyruvate ChEBI	+	carbon source
[Ref.: #31869]	nitrate ChEBI	+	reduction
[Ref.: #31869]	proline ChEBI	+	carbon source
[Ref.: #31869]	raffinose ChEBI	+	carbon source
[Ref.: #31869]	tween 40 ChEBI	+	carbon source
[Ref.: #31869]	tween 80 ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #31869]	alkaline phosphatase	+	3.1.3.1
[Ref.: #31869]	catalase	+	1.11.1.6
[Ref.: #31869]	cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7296]	Sample type/isolated from	activated sludge
[Ref.: #7296]	Geographic location (country and/or sea, region)	Pohang
[Ref.: #7296]	Country	Republic of Korea
[Ref.: #7296]	Country ISO 3 Code	KOR
[Ref.: #7296]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From activated sludge
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Activated sludge

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ372987 (>99% sequence identity) for Simplicispira limi subclade from Microbeatlas

Aquatic Samples	18420
Soil Samples	5888
Animal Samples	3868
Plant Samples	1543
Total Samples	29719

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7296]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7296]	Simplicispira limi strain EMB325 16S ribosomal RNA gene, partial sequence	DQ372987	1455	GenBank ENA	370980 tax ID

[Ref.: #7296]	GC-content	63.3 mol%	high performance liquid chromatography (HPLC)
[Ref.: #31869]	GC-content	63.3 mol%

Availability in culture collections External links

[Ref.: #7296]

Culture collection no.

DSM 17964, KCTC 12608

[Ref.: #72521]

SI-ID 399655

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Simplicispira lacusdiani sp. nov., a novel betaproteobacterium isolated from a freshwater reservoir.	Shi YL, Sun Y, Jiang ZM, Ruan ZY, Su J, Yu LY, Zhang YQ	Int J Syst Evol Microbiol	10.1099/ijsem.0.003112	2018	*
Phylogeny	Simplicispira limi sp. nov., isolated from activated sludge.	Lu S, Ryu SH, Chung BS, Chung YR, Park W, Jeon CO	Int J Syst Evol Microbiol	10.1099/ijs.0.64566-0	2007	*

References

#7296

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17964

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31869

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28129 (see below)

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#72521

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399655.1 )

#28129

IJSEM 31 2007 ( DOI 10.1099/ijs.0.64566-0 , PubMed 17220436 )

* These data were automatically processed and therefore are not curated

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