Brachymonas chironomi AIMA4 | Type strain | DSM 19884, LMG 24400 | BacDiveID:2909

Strain identifier

BacDive ID: 2909

Type strain:

Species: Brachymonas chironomi

Strain Designation: AIMA4

Culture col. no.: DSM 19884, LMG 24400

Strain history: <- M. Halpern, Department of Biology, University of Haifa; AIMA4

NCBI tax ID(s): 1121116 (strain), 491919 (species)

SI-ID 323188

LMG 24400, DSM 19884

Special Collections

Section

Brachymonas chironomi AIMA4 is an aerobe, Gram-negative, ovoid-shaped bacterium that was isolated from chironomid egg mass.

Gram-negative
ovoid-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Comamonadaceae*
	Genus	*Brachymonas*
	Species	**Brachymonas chironomi**
	Full Scientific Name (LPSN)	Brachymonas chironomi Halpern et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #29142]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %97.5

[Ref.: #29142]	Cell length	2 µm

[Ref.: #29142]	Cell width	0.975 µm

[Ref.: #29142]	Cell shape	ovoid-shaped

[Ref.: #29142]	Motility	no

Pigment production

yes

Multimedia content

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Caption:

electron microscopic image

Intellectual property rights:

© HZI/Manfred Rohde

Information on culture and growth conditions Culture and growth conditions

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

Culture medium growth

Culture medium link

Medium recipe at MediaDive

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #8367]			growth	28 °C
[Ref.: #29142]			growth	18-37 °C
[Ref.: #29142]			optimum	30 °C

	pH	Kind of pH		pH
[Ref.: #29142]			optimum	6.0-8.0
[Ref.: #29142]			growth	5.0-9.0

Information on physiology and metabolism Physiology and metabolism

Oxygen tolerance

aerobe

[Ref.: #69480]	Ability of spore formation	no Confidence in %91.62
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29142]		NaCl		growth	0-2.5	%
[Ref.: #29142]		NaCl		optimum	0.5	%

Observation

aggregates in chains

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #29142]		acid phosphatase	+	3.1.3.2
[Ref.: #29142]		alkaline phosphatase	+	3.1.3.1
[Ref.: #29142]		catalase	+	1.11.1.6
[Ref.: #29142]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8367]	Sample type/isolated from	chironomid egg mass
[Ref.: #8367]	Country	Israel
[Ref.: #8367]	Country ISO 3 Code	ISR
[Ref.: #8367]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body Product	#Urogenital tract	#Egg

What are isolation sources categories?

Global distribution of 16S sequence EU346912 (>99% sequence identity) for Brachymonas chironomi subclade from Microbeatlas

Aquatic Samples	679
Soil Samples	19
Animal Samples	292
Plant Samples	19
Total Samples	1009

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

Biosafety level

1

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8367]	Brachymonas chironomi strain AIMA4 16S ribosomal RNA gene, partial sequence	EU346912	1501	GenBank ENA	1121116 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Brachymonas chironomi DSM 19884	GCA_000374625	scaffold	GenBank ENA	1121116 tax ID	*
[Ref.: #66792]	Brachymonas chironomi DSM 19884	1121116.3	wgs	PATRIC	1121116 tax ID	*
[Ref.: #66792]	Brachymonas chironomi DSM 19884	2518285616	draft	JGI IMG/M	1121116 tax ID	*

[Ref.: #8367]	GC-content	60 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29142]	GC-content	60 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Brachymonas chironomi DSM 19884 DSM 19884 NCBI: GCA_000374625.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Brachymonas chironomi DSM 19884 DSM 19884 NCBI: GCA_000374625.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	97.5	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	94.338	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	78.649	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	91.62	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	93.324	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	81.108	yes

Availability in culture collections External links

Culture collection no.

DSM 19884, LMG 24400

SI-ID 323188

*

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	High quality draft genome sequence of Brachymonas chironomi AIMA4(T) (DSM 19884(T)) isolated from a Chironomus sp. egg mass.	Laviad S, Lapidus A, Han J, Haynes M, Reddy T, Huntemann M, Pati A, Ivanova NN, Mavromatis K, Lang E, Rohde M, Markowitz V, Woyke T, Klenk HP, Kyrpides NC, Halpern M	Stand Genomic Sci	10.1186/s40793-015-0010-4	2015	*
Phylogeny	Brachymonas chironomi sp. nov., isolated from a chironomid egg mass, and emended description of the genus Brachymonas.	Halpern M, Shaked T, Schumann P	Int J Syst Evol Microbiol	10.1099/ijs.0.007211-0	2009	*

References

#8367

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19884

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29142

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25566 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72432

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID323188.1 )

#25566

IJSEM 3025 2009 ( DOI 10.1099/ijs.0.007211-0 , PubMed 19643891 )

* These data were automatically processed and therefore are not curated

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