Name: Streptococcus rubneri beta 112/2b
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24609

Type strain:

Species: Streptococcus rubneri

Strain Designation: beta 112/2b

Culture col. no.: DSM 26920, LMG 27207, CCUG 66516

Strain history: <- C. M. A. P. Franz, Max Rubner-Inst. (MRI), Federal Res. Inst. Nutrition and Food, Karlsruhe, Germany; LMG 27207 <- A. Bub and C. M. A. P. Franz

NCBI tax ID(s): 1234680 (species)

SI-ID 310322

LMG 27207, DSM 26920, KCTC 15529

Section

Streptococcus rubneri beta 112/2b is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from throat of a healthy human volunteer.

coccus-shaped
Gram-positive
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Streptococcaceae*
	Genus	*Streptococcus*
	Species	**Streptococcus rubneri**
	Full Scientific Name (LPSN)	Streptococcus rubneri Huch et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30961]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #30961]	Cell shape	coccus-shaped

[Ref.: #69480]	Motility	no Confidence in %94.172

[Ref.: #21039]	Type of hemolysis	alpha
[Ref.: #21039]	Hemolysis ability	1
[Ref.: #21039]	Incubation period	1-2 days

[Ref.: #30961]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21039]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #21039]

Culture medium growth

[Ref.: #21039]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21039]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #21039]	growth	37 °C
[Ref.: #30961]	optimum	25-37 °C
[Ref.: #63344]	growth	37 °C

[Ref.: #21039]	Temperature range	mesophilic
[Ref.: #30961]	Temperature range	mesophilic
[Ref.: #63344]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #21039]	Oxygen tolerance	microaerophile
[Ref.: #30961]	Oxygen tolerance	facultative anaerobe
[Ref.: #63344]	Oxygen tolerance	microaerophile

[Ref.: #30961]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %99
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.96

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30961]	cellobiose ChEBI	+	carbon source
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-mannitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-ribose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-tagatose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #30961]	fructose ChEBI	+	carbon source
[Ref.: #30961]	galactose ChEBI	+	carbon source
[Ref.: #30961]	glucose ChEBI	+	carbon source
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	hippurate ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #68381]	L-arabinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #30961]	lactose ChEBI	+	carbon source
[Ref.: #68381]	lactose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #30961]	maltose ChEBI	+	carbon source
[Ref.: #68381]	maltose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #30961]	mannose ChEBI	+	carbon source
[Ref.: #68381]	melezitose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #30961]	melibiose ChEBI	+	carbon source
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #30961]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #68381]	pullulan ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #30961]	raffinose ChEBI	+	carbon source
[Ref.: #68381]	raffinose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #30961]	salicin ChEBI	+	carbon source
[Ref.: #30961]	sorbitol ChEBI	+	carbon source
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #30961]	sucrose ChEBI	+	carbon source
[Ref.: #68381]	sucrose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	trehalose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	urea ChEBI	-	hydrolysis	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68381]		acetoin ChEBI	no	* from API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68381]		acetoin ChEBI	-	* from API rID32STR

	Enzyme	Enzyme activity	EC number
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	+		* from API rID32STR
[Ref.: #68381]	alkaline phosphatase	-	3.1.3.1	* from API rID32STR
[Ref.: #68381]	alpha-galactosidase	+	3.2.1.22	* from API rID32STR
[Ref.: #68381]	beta-galactosidase	-	3.2.1.23	* from API rID32STR
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR
[Ref.: #68381]	beta-mannosidase	-	3.2.1.25	* from API rID32STR
[Ref.: #21039]	catalase	-	1.11.1.6
[Ref.: #21039]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68381]	glycyl tryptophan arylamidase	+		* from API rID32STR
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32STR
[Ref.: #68381]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32STR
[Ref.: #68381]	urease	-	3.5.1.5	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #21039]	10611	+	+/-	+	-	+	-	-	-	-	+	-	+	+	-	-	+/-	-	+	-	-	-	+	-	-	+	+	+/-	-	-	+	-	-
[Ref.: #21039]	10610	-	-	+	-	+	-	-	-	-	+	-	+	+	-	-	-	-	+	-	-	-	+	-	-	+	+	+/-	-	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21039]	Sample type/isolated from	throat of a healthy human volunteer
[Ref.: #21039]	Geographic location (country and/or sea, region)	Karlsruhe
[Ref.: #21039]	Country	Germany
[Ref.: #21039]	Country ISO 3 Code	DEU
[Ref.: #21039]	Continent	Europe

[Ref.: #63344]	Sample type/isolated from	Throat,healthy human volunteer
[Ref.: #63344]	Geographic location (country and/or sea, region)	Karlsruhe
[Ref.: #63344]	Country	Germany
[Ref.: #63344]	Country ISO 3 Code	DEU
[Ref.: #63344]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Oral cavity and airways	#Throat

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JX861483 (>99% sequence identity) for Streptococcus from Microbeatlas

Aquatic Samples	678
Soil Samples	635
Animal Samples	54450
Plant Samples	421
Total Samples	56184

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21039]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21039]	Streptococcus rubneri strain LMG 27207 16S ribosomal RNA gene, partial sequence	JX861483	1457	ENA	1234680 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Streptococcus rubneri DSM 26920	GCA_004785935	contig	GenBank	1234680 tax ID	*
[Ref.: #66792]	Streptococcus rubneri CCUG 66516	GCA_016642415	scaffold	GenBank	1234680 tax ID	*
[Ref.: #66792]	Streptococcus rubneri strain CCUG 66516	1234680.7	wgs	PATRIC	1234680 tax ID	*
[Ref.: #66792]	Streptococcus rubneri strain DSM 26920	1234680.3	wgs	PATRIC	1234680 tax ID	*

[Ref.: #21039]

GC-content

40.9 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Streptococcus rubneri strain DSM 26920 PATRIC: 1234680.8
[Ref.: #69481]	spore-forming	no	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Streptococcus rubneri CCUG 66516 NCBI: GCA_016642415.1
[Ref.: #69480]	flagellated	no	95.956	no
[Ref.: #69480]	gram-positive	yes	95.421	no
[Ref.: #69480]	anaerobic	no	93.55	yes
[Ref.: #69480]	aerobic	no	97.181	no
[Ref.: #69480]	halophile	yes	67.327	no
[Ref.: #69480]	spore-forming	no	92.337	yes
[Ref.: #69480]	thermophile	no	99.346	no
[Ref.: #69480]	glucose-util	yes	86.713	yes
[Ref.: #69480]	motile	no	94.236	no
[Ref.: #69480]	glucose-ferment	yes	84.473	no

Availability in culture collections External links

[Ref.: #21039]

Culture collection no.

DSM 26920, LMG 27207, CCUG 66516

[Ref.: #88454]

SI-ID 310322

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Streptococcus rubneri sp. nov., isolated from the human throat.	Huch M, De Bruyne K, Cleenwerck I, Bub A, Cho GS, Watzl B, Snauwaert I, Franz CMAP, Vandamme P	Int J Syst Evol Microbiol	10.1099/ijs.0.048538-0	2013	*
Phylogeny	Streptococcus xiaochunlingii sp. nov. E24 Isolated From the Oropharynx of Healthy Chinese Children.	Zou Y, Sun Y, Qi H, Liu D, Tian H, Wang N, Li X	Front Microbiol	10.3389/fmicb.2020.563213	2020	*
Phylogeny	Streptococcus koreensis sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion.	Lim YK, Park SN, Shin JH, Ji S, Jo E, Chang YH, Shin Y, Paek J, Kim H, Kook JK	Curr Microbiol	10.1007/s00284-019-01778-6	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21039

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26920

#30961

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27291 (see below)

#63344 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 66516

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68381

Automatically annotated from API rID32STR .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88454

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID310322.1 )

#27291

IJSEM 4026 2013 ( DOI 10.1099/ijs.0.048538-0 , PubMed 23749274 )

* These data were automatically processed and therefore are not curated

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Streptococcus rubneri strain DSM 26920

Streptococcus rubneri CCUG 66516

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