Planktomarina temperata (DSM 22400, JCM 18269, RCA 23)

Name: Planktomarina temperata (DSM 22400, JCM 18269, RCA 23)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 24527

Type strain

Culture col. no. DSM 22400 JCM 18269 RCA 23

NCBI tax ID(s) 1284658

Special Collections DSMZ JCM Literature strains North Sea Microbiome

History <- H.-A. Giebel and T. Brinkhoff, ICMB, Univ. Oldenburg, Germany; RCA23 <- R. Gahl-Janßen and H.-A. Giebel

Links

Planktomarina temperata DSM 22400 is an aerobe, psychrophilic, Gram-negative prokaryote that was isolated from surface seawater.

Gram-negative motile rod-shaped aerobe psychrophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 405603

DSM 22400

@ref 20215

Last LPSN update 2026-02-09 11:24:00

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodobacterales

Family Roseobacteraceae

Genus Planktomarina

Species Planktomarina temperata

Full scientific name Planktomarina temperata Giebel et al. 2013

Synonyms (1)

"Candidatus Planktomarina temperata"

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
31138	negative	2.15 µm	0.85 µm	rod-shaped

Colony morphology

21289

Incubation period2-3 days

Pigmentation

31138

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
21289	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)		Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Sea water Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
21289	positive	growth	20	psychrophilic
31138	positive	growth	10-30
31138	positive	optimum	25	mesophilic

Culture pH

@ref	Ability	Type	PH
31138	positive	optimum	7.5
31138	positive	growth	07-08

Physiology and metabolism

Oxygen tolerance

31138

Oxygen toleranceaerobe

Spore formation

31138

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31138	NaCl	positive	growth	1.5-5.0 %
31138	NaCl	positive	optimum	3.25 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31138	30089 ChEBI	acetate	+	carbon source
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
31138	16449 ChEBI	alanine	+	carbon source
31138	22599 ChEBI	arabinose	+	carbon source
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
31138	17057 ChEBI	cellobiose	+	carbon source
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
31138	15740 ChEBI	formate	+	carbon source
31138	28757 ChEBI	fructose	+	carbon source
31138	33984 ChEBI	fucose	+	carbon source
31138	28260 ChEBI	galactose	+	carbon source
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
31138	17234 ChEBI	glucose	+	carbon source
31138	29987 ChEBI	glutamate	+	carbon source
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
31138	25115 ChEBI	malate	+	carbon source
68369	25115 ChEBI	malate	-	assimilation	from API 20NE
31138	17306 ChEBI	maltose	+	carbon source
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
31138	37684 ChEBI	mannose	+	carbon source
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
31138	28044 ChEBI	phenylalanine	+	carbon source
31138	17272 ChEBI	propionate	+	carbon source
31138	17148 ChEBI	putrescine	+	carbon source
31138	15361 ChEBI	pyruvate	+	carbon source
31138	26546 ChEBI	rhamnose	+	carbon source
31138	33942 ChEBI	ribose	+	carbon source
31138	17822 ChEBI	serine	+	carbon source
31138	30031 ChEBI	succinate	+	carbon source
31138	27082 ChEBI	trehalose	+	carbon source
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE
31138	31011 ChEBI	valerate	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
31138	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
31138	cytochrome oxidase	+	1.9.3.1
68382	esterase lipase (C 8)	-		from API zym
68369	gelatinase	-		from API 20NE
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68382	trypsin	-	3.4.21.4	from API zym
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
21289	-	+	+/-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
21289	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Surface water

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
21289	surface seawater	southern North Sea, German Wadden Sea, Neuharlinger Siel (53° 42' 20'' N; 07° 43' 11'' E)	Germany	DEU	Europe	53.7056	7.7197 53.7056/7.7197

Taxonmaps

69479

Global distribution of 16S sequence GQ369962 (>99% sequence identity) for Planktomarina temperata subclade from Microbeatlas

Aquatic Samples	8583
Soil Samples	2006
Animal Samples	253
Plant Samples	168
Total Samples	11010

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
21289	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM73843v1 assembly for Planktomarina temperata RCA23	complete	GCA_000738435	666509.3	2548877138	666509	98.99

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21289	Planktomarina temperata RCA23 16S ribosomal RNA gene, partial sequence	GQ369962	1441		666509

GC content

@ref	GC-content (mol%)	Method
21289	53.67	sequence analysis
31138	53.67

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	The green impact: bacterioplankton response toward a phytoplankton spring bloom in the southern North Sea assessed by comparative metagenomic and metatranscriptomic approaches.	Wemheuer B, Wemheuer F, Hollensteiner J, Meyer FD, Voget S, Daniel R.	Front Microbiol	10.3389/fmicb.2015.00805	2015
Genetics	Trajectories and Drivers of Genome Evolution in Surface-Associated Marine Phaeobacter.	Freese HM, Sikorski J, Bunk B, Scheuner C, Meier-Kolthoff JP, Sproer C, Gram L, Overmann J.	Genome Biol Evol	10.1093/gbe/evx249	2017
Metabolism	Conserved small mRNA with an unique, extended Shine-Dalgarno sequence.	Hahn J, Thalmann S, Migur A, von Boeselager RF, Kubatova N, Kubareva E, Schwalbe H, Evguenieva-Hackenberg E.	RNA Biol	10.1080/15476286.2016.1256534	2017
Metabolism	Complementary energy acquisition via aerobic anoxygenic photosynthesis and carbon monoxide oxidation by Planktomarina temperata of the Roseobacter group.	Giebel HA, Wolterink M, Brinkhoff T, Simon M	FEMS Microbiol Ecol	10.1093/femsec/fiz050	2019
Phylogeny	Adaptation of an abundant Roseobacter RCA organism to pelagic systems revealed by genomic and transcriptomic analyses.	Voget S, Wemheuer B, Brinkhoff T, Vollmers J, Dietrich S, Giebel HA, Beardsley C, Sardemann C, Bakenhus I, Billerbeck S, Daniel R, Simon M	ISME J	10.1038/ismej.2014.134	2014
Phylogeny	Planktomarina temperata gen. nov., sp. nov., belonging to the globally distributed RCA cluster of the marine Roseobacter clade, isolated from the German Wadden Sea.	Giebel HA, Kalhoefer D, Gahl-Janssen R, Choo YJ, Lee K, Cho JC, Tindall BJ, Rhiel E, Beardsley C, Aydogmus OO, Voget S, Daniel R, Simon M, Brinkhoff T	Int J Syst Evol Microbiol	10.1099/ijs.0.053249-0	2013

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21289	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22400
#27466	IJSEM 4207 2013 ( DOI 10.1099/ijs.0.053249-0 , PubMed 23793856 )
#31138	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27466
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Planktomarina temperata (DSM 22400, JCM 18269, RCA 23)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000738435

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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