Name: Planktomarina temperata DSM 22400
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24527

Type strain:

Species: Planktomarina temperata

Culture col. no.: DSM 22400, JCM 18269, RCA 23

Strain history: <- H.-A. Giebel and T. Brinkhoff, ICMB, Univ. Oldenburg, Germany; RCA23 <- R. Gahl-Janßen and H.-A. Giebel

NCBI tax ID(s): 1284658 (species)

SI-ID 405603

DSM 22400

Section

Planktomarina temperata DSM 22400 is an aerobe, psychrophilic, Gram-negative bacterium that was isolated from surface seawater.

Gram-negative
motile
rod-shaped
aerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodobacterales*
	Family	*Roseobacteraceae*
	Genus	*Planktomarina*
	Species	**Planktomarina temperata**
	Full Scientific Name (LPSN)	Planktomarina temperata Giebel et al. 2013

Synonym

"Candidatus Planktomarina temperata"

Information on morphological and physiological properties Morphology

[Ref.: #31138]	Gram stain	negative

[Ref.: #31138]	Cell length	2.15 µm

[Ref.: #31138]	Cell width	0.85 µm

[Ref.: #31138]	Cell shape	rod-shaped

[Ref.: #31138]	Motility	yes

[Ref.: #21289]

Incubation period

2-3 days

[Ref.: #31138]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21289]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #21289]

Culture medium growth

[Ref.: #21289]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21289]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #21289]	growth	20 °C
[Ref.: #31138]	growth	10-30 °C
[Ref.: #31138]	optimum	25 °C

[Ref.: #21289]	Temperature range	psychrophilic
[Ref.: #31138]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31138]			optimum	7.5
[Ref.: #31138]			growth	07-08

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31138]

Oxygen tolerance

aerobe

[Ref.: #31138]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31138]		NaCl		growth	1.5-5.0	%
[Ref.: #31138]		NaCl		optimum	3.25	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31138]	acetate ChEBI	+	carbon source
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #31138]	alanine ChEBI	+	carbon source
[Ref.: #31138]	arabinose ChEBI	+	carbon source
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #31138]	cellobiose ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #31138]	formate ChEBI	+	carbon source
[Ref.: #31138]	fructose ChEBI	+	carbon source
[Ref.: #31138]	fucose ChEBI	+	carbon source
[Ref.: #31138]	galactose ChEBI	+	carbon source
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #31138]	glucose ChEBI	+	carbon source
[Ref.: #31138]	glutamate ChEBI	+	carbon source
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #31138]	malate ChEBI	+	carbon source
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #31138]	maltose ChEBI	+	carbon source
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #31138]	mannose ChEBI	+	carbon source
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #31138]	phenylalanine ChEBI	+	carbon source
[Ref.: #31138]	propionate ChEBI	+	carbon source
[Ref.: #31138]	putrescine ChEBI	+	carbon source
[Ref.: #31138]	pyruvate ChEBI	+	carbon source
[Ref.: #31138]	rhamnose ChEBI	+	carbon source
[Ref.: #31138]	ribose ChEBI	+	carbon source
[Ref.: #31138]	serine ChEBI	+	carbon source
[Ref.: #31138]	succinate ChEBI	+	carbon source
[Ref.: #31138]	trehalose ChEBI	+	carbon source
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #31138]	valerate ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31138]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31138]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #21289]	24816	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #21289]	14149	-	+	+/-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21289]	Sample type/isolated from	surface seawater
[Ref.: #21289]	Geographic location (country and/or sea, region)	southern North Sea, German Wadden Sea, Neuharlinger Siel (53° 42' 20'' N; 07° 43' 11'' E)
[Ref.: #21289]	Country	Germany
[Ref.: #21289]	Country ISO 3 Code	DEU
[Ref.: #21289]	Continent	Europe
[Ref.: #21289]	Geographic location	53.7056°/7.7197°
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Surface water

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence GQ369962 (>99% sequence identity) for Planktomarina temperata subclade from Microbeatlas

Aquatic Samples	8583
Soil Samples	2006
Animal Samples	253
Plant Samples	168
Total Samples	11010

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21289]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21289]	Planktomarina temperata RCA23 16S ribosomal RNA gene, partial sequence	GQ369962	1441	ENA	666509 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Planktomarina temperata RCA23, DSM 22400	2548877138	complete	JGI IMG/M	666509 tax ID	*

[Ref.: #21289]	GC-content	53.67 mol%	sequence analysis
[Ref.: #31138]	GC-content	53.67 mol%

Availability in culture collections External links

[Ref.: #21289]

Culture collection no.

DSM 22400, JCM 18269, RCA 23

[Ref.: #88382]

SI-ID 405603

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Complementary energy acquisition via aerobic anoxygenic photosynthesis and carbon monoxide oxidation by Planktomarina temperata of the Roseobacter group.	Giebel HA, Wolterink M, Brinkhoff T, Simon M	FEMS Microbiol Ecol	10.1093/femsec/fiz050	2019	*
Phylogeny	Adaptation of an abundant Roseobacter RCA organism to pelagic systems revealed by genomic and transcriptomic analyses.	Voget S, Wemheuer B, Brinkhoff T, Vollmers J, Dietrich S, Giebel HA, Beardsley C, Sardemann C, Bakenhus I, Billerbeck S, Daniel R, Simon M	ISME J	10.1038/ismej.2014.134	2014	*
Phylogeny	Planktomarina temperata gen. nov., sp. nov., belonging to the globally distributed RCA cluster of the marine Roseobacter clade, isolated from the German Wadden Sea.	Giebel HA, Kalhoefer D, Gahl-Janssen R, Choo YJ, Lee K, Cho JC, Tindall BJ, Rhiel E, Beardsley C, Aydogmus OO, Voget S, Daniel R, Simon M, Brinkhoff T	Int J Syst Evol Microbiol	10.1099/ijs.0.053249-0	2013	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21289

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22400

#31138

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27466 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#88382

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405603.1 )

#27466

IJSEM 4207 2013 ( DOI 10.1099/ijs.0.053249-0 , PubMed 23793856 )

* These data were automatically processed and therefore are not curated

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