Name: Kaistia hirudinis E94
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24471

Type strain:

Species: Kaistia hirudinis

Strain Designation: E94

Culture col. no.: DSM 25966, CCM 8401, CIP 110381, LMG 26925

Strain history: CIP <- 2012, P. Kämpfer, Giessen Univ., Giessen, Germany: strain E94

NCBI tax ID(s): 1293440 (species)

SI-ID 379504

LMG 26925, CIP 110381, CCM 8401, DSM 25966

Section

Kaistia hirudinis E94 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from skin of a juvenile medicinal leech .

Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Kaistiaceae*
	Genus	*Kaistia*
	Species	**Kaistia hirudinis**
	Full Scientific Name (LPSN)	Kaistia hirudinis Glaeser et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #31006]	Gram stain	negative
[Ref.: #119914]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.992

[Ref.: #31006]	Cell length	1 µm

[Ref.: #31006]	Cell width	0.5 µm

[Ref.: #31006]	Cell shape	rod-shaped
[Ref.: #119914]	Cell shape	oval-shaped

[Ref.: #119914]	Motility	yes

[Ref.: #20615]

Incubation period

2-3 days

[Ref.: #31006]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #34223]

Culture medium

MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromati

[Ref.: #34223]

Culture medium growth

[Ref.: #34223]

Culture medium composition

expand / minimize

[Ref.: #20615]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #20615]

Culture medium growth

[Ref.: #20615]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20615]

Culture medium composition

expand / minimize

[Ref.: #119914]

Culture medium

CIP Medium 566

[Ref.: #119914]

Culture medium growth

[Ref.: #119914]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #20615]	growth	25 °C
[Ref.: #31006]	growth	15-37 °C
[Ref.: #34223]	growth	25 °C

[Ref.: #20615]	Temperature range	mesophilic
[Ref.: #34223]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31006]			optimum	7
[Ref.: #31006]			growth	4.5-10.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31006]	Oxygen tolerance	aerobe
[Ref.: #119914]	Oxygen tolerance	obligate aerobe

[Ref.: #31006]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.989

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31006]		NaCl		growth	01-10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31006]	3-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #31006]	acetate ChEBI	+	carbon source
[Ref.: #31006]	alanine ChEBI	+	carbon source
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31006]	lactate ChEBI	+	carbon source
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31006]	nitrate ChEBI	+	reduction
[Ref.: #119914]	nitrate ChEBI	+	reduction
[Ref.: #119914]	nitrite ChEBI	-	reduction
[Ref.: #31006]	phenylacetate ChEBI	+	carbon source
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31006]	proline ChEBI	+	carbon source
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #119914]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #31006]	catalase	+	1.11.1.6
[Ref.: #119914]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31006]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119914]	oxidase	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #119914]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #119914]	11765	not determinedn.d.	-	-	+/-	+/-	+/-	+/-	-	-	-	+/-	+/-	+/-	+/-	-	+/-	-	-	-	-	-	+/-	-	-	+/-	-	+/-	+/-	+/-	-	-	+/-	+/-	-	+/-	+/-	-	-	-	+/-	+/-	+/-	-	+/-	+/-	+/-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119914]	12561	-	-	+	-	-	+	-	-	+	-	+	+	-	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20615]	Sample type/isolated from	skin of a juvenile medicinal leech (Hirudo verbana)
[Ref.: #20615]	Host species	Hirudo verbana
[Ref.: #20615]	Geographic location (country and/or sea, region)	Hessen, Biebertal, Leech Breeding Station
[Ref.: #20615]	Country	Germany
[Ref.: #20615]	Country ISO 3 Code	DEU
[Ref.: #20615]	Continent	Europe

[Ref.: #119914]	Sample type/isolated from	Animal, Surface of a juvenile leech, Hirudo verbana
[Ref.: #119914]	Geographic location (country and/or sea, region)	Biebertal, Hessen
[Ref.: #119914]	Country	Germany
[Ref.: #119914]	Country ISO 3 Code	DEU
[Ref.: #119914]	Continent	Europe
[Ref.: #119914]	Isolation date	2009
* marker position based on {}

Isolation sources categories

#Host	#Invertebrates (Other)	#Annelida
#Host Body-Site	#Organ	#Skin, Nail, Hair

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KC254734 (>99% sequence identity) for Hyphomicrobiales from Microbeatlas

Aquatic Samples	150
Soil Samples	34
Animal Samples	22
Plant Samples	105
Total Samples	311

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20615]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119914]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20615]	Kaistia hirudinis strain E94 16S ribosomal RNA gene, partial sequence	KC254734	1308	ENA	1293440 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Kaistia hirudinis DSM 25966	GCA_014196455	contig	GenBank	1293440 tax ID	*
[Ref.: #66792]	Kaistia hirudinis strain DSM 25966	1293440.3	wgs	PATRIC	1293440 tax ID	*
[Ref.: #66792]	Kaistia hirudinis DSM 25966	2829893694	draft	JGI IMG/M	1293440 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Kaistia hirudinis strain DSM 25966 PATRIC: 1293440.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Kaistia hirudinis strain DSM 25966 PATRIC: 1293440.3
[Ref.: #69480]	motile	no	91.248	no
[Ref.: #69480]	gram-positive	no	97.633	yes
[Ref.: #69480]	anaerobic	no	98.595	yes
[Ref.: #69480]	aerobic	yes	90.699	yes
[Ref.: #69480]	halophile	no	90.94	no
[Ref.: #69480]	spore-forming	no	96.635	yes
[Ref.: #69480]	glucose-util	yes	92.365	no
[Ref.: #69480]	flagellated	no	97.011	no
[Ref.: #69480]	thermophile	no	96.777	yes
[Ref.: #69480]	glucose-ferment	no	91.606	no

Availability in culture collections External links

[Ref.: #20615]

Culture collection no.

DSM 25966, CCM 8401, CIP 110381, LMG 26925

[Ref.: #88340]

SI-ID 379504

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Kaistia hirudinis sp. nov., isolated from the skin of Hirudo verbana.	Glaeser SP, Galatis H, Martin K, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.049619-0	2013	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20615

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25966

#31006

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27336 (see below)

#34223 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88340

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID379504.1 )

#119914 Collection of Institut Pasteur ; Curators of the CIP; CIP 110381

#27336

IJSEM 3209 2013 ( DOI 10.1099/ijs.0.049619-0 , PubMed 23456803 )

* These data were automatically processed and therefore are not curated

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Kaistia hirudinis strain DSM 25966

Kaistia hirudinis strain DSM 25966

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