Name: Methylobacterium brachythecii 99b
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24174

Type strain:

Species: Methylobacterium brachythecii

Strain Designation: 99b

Culture col. no.: DSM 24105, NBRC 107710

Strain history: <- A. Tani, Okayama University, Japan; 99b

NCBI tax ID(s): 1176177 (species)

SI-ID 397582

DSM 24105

Section

Methylobacterium brachythecii 99b is an aerobe, mesophilic, Gram-negative bacterium that was isolated from rusty feather-moss Brachythecium plumosum.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Methylobacteriaceae*
	Genus	*Methylobacterium*
	Species	**Methylobacterium brachythecii**
	Full Scientific Name (LPSN)	Methylobacterium brachythecii Tani and Sahin 2013

Information on morphological and physiological properties Morphology

[Ref.: #30947]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.998

[Ref.: #30947]	Cell length	2.5 µm

[Ref.: #30947]	Cell width	1.2 µm

[Ref.: #30947]	Cell shape	rod-shaped

[Ref.: #30947]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %95.666

[Ref.: #20649]

Incubation period

2-3 days

[Ref.: #30947]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20649]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #20649]

Culture medium growth

[Ref.: #20649]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20649]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #20649]			growth	28 °C

[Ref.: #20649]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30947]

Oxygen tolerance

aerobe

[Ref.: #30947]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.993

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30947]	acetate ChEBI	+	carbon source
[Ref.: #30947]	arabinose ChEBI	+	carbon source
[Ref.: #30947]	asparagine ChEBI	+	carbon source
[Ref.: #30947]	aspartate ChEBI	+	carbon source
[Ref.: #30947]	dextrin ChEBI	+	carbon source
[Ref.: #30947]	formate ChEBI	+	carbon source
[Ref.: #30947]	glucose ChEBI	+	carbon source
[Ref.: #30947]	glutamate ChEBI	+	carbon source
[Ref.: #30947]	glycerol ChEBI	+	carbon source
[Ref.: #30947]	glycogen ChEBI	+	carbon source
[Ref.: #30947]	lactate ChEBI	+	carbon source
[Ref.: #30947]	malonate ChEBI	+	carbon source
[Ref.: #30947]	methyl pyruvate ChEBI	+	carbon source
[Ref.: #30947]	nitrate ChEBI	+	reduction
[Ref.: #30947]	propionate ChEBI	+	carbon source
[Ref.: #30947]	succinate ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #30947]	catalase	+	1.11.1.6
[Ref.: #30947]	cytochrome oxidase	+	1.9.3.1
[Ref.: #30947]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20649]	Sample type/isolated from	rusty feather-moss Brachythecium plumosum
[Ref.: #20649]	Host species	Brachythecium plumosum
[Ref.: #20649]	Geographic location (country and/or sea, region)	premises of the Institute of Plant Science and Resources, Okayama University
[Ref.: #20649]	Country	Japan
[Ref.: #20649]	Country ISO 3 Code	JPN
[Ref.: #20649]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host

#Plants

#Moss

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20649]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20649]	Methylobacterium brachythecii gene for 16S rRNA, partial sequence, strain: 99b	AB703239	1444	ENA	1176177 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methylobacterium brachythecii DSM 24105	GCA_014196345	contig	GenBank	1176177 tax ID	*
[Ref.: #66792]	Methylobacterium brachythecii strain DSM 24105	1176177.3	wgs	PATRIC	1176177 tax ID	*
[Ref.: #66792]	Methylobacterium brachythecii DSM 24105	2829871319	draft	JGI IMG/M	1176177 tax ID	*

[Ref.: #20649]	GC-content	66.5 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #30947]	GC-content	66.5 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Methylobacterium brachythecii strain DSM 24105 PATRIC: 1176177.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methylobacterium brachythecii strain DSM 24105 PATRIC: 1176177.3
[Ref.: #69480]	motile	yes	86.696	yes
[Ref.: #69480]	gram-positive	no	98.076	yes
[Ref.: #69480]	anaerobic	no	98.268	no
[Ref.: #69480]	halophile	no	94.284	no
[Ref.: #69480]	spore-forming	no	92.35	yes
[Ref.: #69480]	flagellated	no	73.001	no
[Ref.: #69480]	thermophile	no	99.456	yes
[Ref.: #69480]	glucose-util	yes	90.292	no
[Ref.: #69480]	aerobic	yes	94.158	no
[Ref.: #69480]	glucose-ferment	no	91.122	no

Availability in culture collections External links

[Ref.: #20649]

Culture collection no.

DSM 24105, NBRC 107710

[Ref.: #88129]

SI-ID 397582

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methylobacterium haplocladii sp. nov. and Methylobacterium brachythecii sp. nov., isolated from bryophytes.	Tani A, Sahin N	Int J Syst Evol Microbiol	10.1099/ijs.0.048215-0	2013	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20649

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24105

#30947

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27277 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88129

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397582.1 )

#27277

IJSEM 3287 2013 ( DOI 10.1099/ijs.0.048215-0 , PubMed 23475343 )

* These data were automatically processed and therefore are not curated

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Methylobacterium brachythecii strain DSM 24105

Methylobacterium brachythecii strain DSM 24105

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