Methanohalophilus levihalophilus (DSM 28452, NBRC 110099, GTA13)

Name: Methanohalophilus levihalophilus (DSM 28452, NBRC 110099, GTA13)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 24163

Type strain

Strain Designation GTA13

Culture col. no. DSM 28452 NBRC 110099 GTA13

NCBI tax ID(s) 1431282

Special Collections DSMZ

History DSMZ <- T. Katayama, Natl. Inst. Advanced Industrial Sci. and Technol., Tsukuba, Japan; GTA13 <- T. Katayama {2013}

Links

Methanohalophilus levihalophilus GTA13 is an anaerobe archaeon that was isolated from deep aquifers filled with paleo-seawater in natural gas field.

anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 398713

NBRC 110099,DSM 28452

@ref 20215

Last LPSN update 2026-05-29 08:30:34

Domain Archaea

Phylum Methanobacteriota

Class Methanosarcinia

Order Methanosarcinales

Family Methanosarcinaceae

Genus Methanohalophilus

Species Methanohalophilus levihalophilus

Full scientific name Methanohalophilus levihalophilus Katayama et al. 2014

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	97.36

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
21018	METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c)		Medium recipe at MediaDive	Name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c) Composition: NaHCO3 4.89237 g/l Trimethylammonium chloride 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Biotin 1.95695e-05 g/l Folic acid 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
21018	positive	growth	35

Physiology and metabolism

Oxygen tolerance

21018

Oxygen toleranceanaerobe

Spore formation

@ref	Spore formation	Confidence
125439		99.816

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Groundwater
#Environmental	#Aquatic	#Marine

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
21018	deep aquifers filled with paleo-seawater in natural gas field	Chiba Prefecture, Kujukuri Town, Minami-Kanto gas field	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AB889605 (>99% sequence identity) for Methanohalophilus levihalophilus subclade from Microbeatlas

Aquatic Samples	6
Soil Samples	0
Animal Samples	0
Plant Samples	0
Total Samples	6

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
21018	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1787437v1 assembly for Methanohalophilus levihalophilus DSM 28452	contig	GCA_017874375	1431282.3	2913510308	1431282	77.3

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21018	Methanohalophilus levihalophilus gene for 16S ribosomal RNA, partial sequence	AB889605	1369		1431282

GC content

@ref	GC-content (mol%)	Method
21018	43.7	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1787437v1 assembly for Methanohalophilus levihalophilus DSM 28452
NCBI: GCA_017874375

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	53.26	no
125439	motility	BacteriaNetⓘ	no	81.74	no
125439	gram_stain	BacteriaNetⓘ	negative	97.36	no
125439	spore_formation	BacteriaNetⓘ	no	99.82	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanohalophilus levihalophilus sp. nov., a slightly halophilic, methylotrophic methanogen isolated from natural gas-bearing deep aquifers, and emended description of the genus Methanohalophilus.	Katayama T, Yoshioka H, Mochimaru H, Meng XY, Muramoto Y, Usami J, Ikeda H, Kamagata Y, Sakata S	Int J Syst Evol Microbiol	10.1099/ijs.0.063677-0	2014

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21018	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28452
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanohalophilus levihalophilus (DSM 28452, NBRC 110099, GTA13)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_017874375

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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