Name: Lysinibacillus pakistanensis DSM 24784
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 23762

Type strain:

Species: Lysinibacillus pakistanensis

Culture col. no.: DSM 24784, JCM 18776, KCTC 13795, NCCP 54

Strain history: <- I Ahmed, NCCP, Pakistan

NCBI tax ID(s): 759811 (species)

SI-ID 400013

DSM 24784

Section

Lysinibacillus pakistanensis DSM 24784 is an aerobe, spore-forming, mesophilic bacterium that was isolated from rhizospheric soil of soybean Glycine max L..

motile
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Caryophanaceae*
	Genus	*Lysinibacillus*
	Species	**Lysinibacillus pakistanensis**
	Full Scientific Name (LPSN)	Lysinibacillus pakistanensis Ahmed et al. 2014

Information on morphological and physiological properties Morphology

[Ref.: #67771]	Gram stain	positive

[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #67771]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21169]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #21169]

Culture medium growth

[Ref.: #21169]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21169]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #21169]	growth	30 °C
[Ref.: #67770]	growth	30 °C
[Ref.: #67771]	growth	28 °C

[Ref.: #21169]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]

Oxygen tolerance

aerobe

[Ref.: #67771]	Type of spore	endospore
[Ref.: #67771]	Ability of spore formation	yes

[Ref.: #21169]	Murein short key	A11.31
[Ref.: #21169]	Murein types	A4alpha L-Lys-D-Asp

[Ref.: #67770]	Observation	quinones: MK-7
[Ref.: #67771]	Observation	quinones: MK-7

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21169]	Sample type/isolated from	rhizospheric soil of soybean Glycine max L.
[Ref.: #21169]	Host species	Glycine max
[Ref.: #21169]	Geographic location (country and/or sea, region)	Rawaldpindi, Research Farm of Pir Mehr Ali Shah Arid Agriculture University
[Ref.: #21169]	Country	Pakistan
[Ref.: #21169]	Country ISO 3 Code	PAK
[Ref.: #21169]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Rhizospheric soil of soybean (Glycine max L.)
[Ref.: #67770]	Host species	Glycine max
[Ref.: #67770]	Geographic location (country and/or sea, region)	PMAS Arid Agric. Univ., Rawalpindi
[Ref.: #67770]	Country	Pakistan
[Ref.: #67770]	Country ISO 3 Code	PAK
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From legume (`Glycine max`) rhizospheric soil
[Ref.: #67771]	Geographic location (country and/or sea, region)	Research farm, PMAS Arid Agriculture University, Rawalpindi
[Ref.: #67771]	Country	Pakistan
[Ref.: #67771]	Country ISO 3 Code	PAK
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Rhizosphere

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21169]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21169]	Lysinibacillus pakistanensis gene for 16S rRNA, partial sequence	AB558495	1481	ENA	759811 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lysinibacillus pakistanensis JCM 18776	GCA_001312325	contig	GenBank	759811 tax ID
[Ref.: #66792]	Lysinibacillus pakistanensis strain JCM 18776	759811.5	wgs	PATRIC	759811 tax ID	*
[Ref.: #66792]	Lysinibacillus pakistanensis JCM 18776	2728369720	draft	JGI IMG/M	759811 tax ID	*

[Ref.: #21169]	GC-content	37 mol%
[Ref.: #67770]	GC-content	37 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	37.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lysinibacillus pakistanensis strain JCM 18776 PATRIC: 759811.5
[Ref.: #69480]	motile	yes	87.757	no
[Ref.: #69480]	flagellated	yes	80.068	no
[Ref.: #69480]	gram-positive	yes	89.966	yes
[Ref.: #69480]	anaerobic	no	96.44	yes
[Ref.: #69480]	aerobic	yes	87.481	yes
[Ref.: #69480]	halophile	no	95.127	no
[Ref.: #69480]	spore-forming	yes	97.387	yes
[Ref.: #69480]	glucose-util	yes	82.845	no
[Ref.: #69480]	thermophile	no	99.855	no
[Ref.: #69480]	glucose-ferment	no	91.676	no

Availability in culture collections External links

[Ref.: #21169]

Culture collection no.

DSM 24784, JCM 18776, KCTC 13795, NCCP 54

[Ref.: #87798]

SI-ID 400013

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	FRET-based fluorescent nanoprobe platform for sorting of active microorganisms by functional properties.	Luo Y, Liu F, Li E, Fang Y, Zhao G, Dai X, Li J, Wang B, Xu M, Liao B, Sun G	Biosens Bioelectron	10.1016/j.bios.2019.111832	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21169

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24784

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#87798

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400013.1 )

* These data were automatically processed and therefore are not curated

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Lysinibacillus pakistanensis strain JCM 18776

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