Marinilactibacillus piezotolerans (DSM 16108, LT20, CIP 108666)

Name: Marinilactibacillus piezotolerans (DSM 16108, LT20, CIP 108666)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2278

Type strain

Strain Designation LT20

Culture col. no. DSM 16108 LT20 CIP 108666 JCM 12337

NCBI tax ID(s) 258723

Special Collections DSMZ JCM CIP Literature strains

Links

Marinilactibacillus piezotolerans LT20 is a facultative anaerobe, mesophilic, Gram-positive prokaryote that was isolated from marine sediment .

Gram-positive rod-shaped facultative anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 135120

DSM 16108,JCM 12337,CIP 108666

@ref 20215

Last LPSN update 2025-12-16 09:47:16

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Carnobacteriaceae

Genus Marinilactibacillus

Species Marinilactibacillus piezotolerans

Full scientific name Marinilactibacillus piezotolerans Toffin et al. 2005

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
31295	positive	2.1 µm	0.325 µm	rod-shaped
121829	positive			rod-shaped

Colony morphology

@ref	Incubation period
6298	3-7 days
121829

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6298	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)	Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l Glucose 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
34008	Marine agar (MA)		Distilled water make up to (1000.000 ml);Marine agar (55.100 g)
121829	CIP Medium 72	Medium recipe at CIP
121829	CIP Medium 13	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6298	positive	growth	30	mesophilic
31295	positive	growth	04-50
31295	positive	optimum	38.5	mesophilic
34008	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic
121829	positive	growth	15-37
121829	negative	growth	5
121829	negative	growth	41

Culture pH

@ref	Ability	Type	PH
31295	positive	optimum	7.5
31295	positive	growth	5.5-10

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
6298	facultative anaerobe
31295	facultative anaerobe
121829	facultative anaerobe
125439	obligate aerobe

Spore formation

31295

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31295	NaCl	positive	growth	0-11.91 %
31295	NaCl	positive	optimum	1.49 %
121829	NaCl	positive	growth	2-10 %
121829	NaCl		growth	0 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31295	30089 ChEBI	acetate	+	carbon source
31295	17057 ChEBI	cellobiose	+	carbon source
121829	16947 ChEBI	citrate	-	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
121829	4853 ChEBI	esculin	+	hydrolysis
31295	16236 ChEBI	ethanol	+	carbon source
31295	28757 ChEBI	fructose	+	carbon source
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
31295	28260 ChEBI	galactose	+	carbon source
31295	17234 ChEBI	glucose	+	carbon source
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
121829	606565 ChEBI	hippurate	+	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
31295	17716 ChEBI	lactose	+	carbon source
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
121829	15792 ChEBI	malonate	-	assimilation
31295	17306 ChEBI	maltose	+	carbon source
31295	29864 ChEBI	mannitol	+	carbon source
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
121829	17632 ChEBI	nitrate	-	builds gas from
121829	17632 ChEBI	nitrate	-	reduction
121829	16301 ChEBI	nitrite	-	builds gas from
121829	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
31295	30911 ChEBI	sorbitol	+	carbon source
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
31295	17992 ChEBI	sucrose	+	carbon source
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid
31295	18222 ChEBI	xylose	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
121829	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
121829	alcohol dehydrogenase	-	1.1.1.1
31295	alkaline phosphatase	+	3.1.3.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
121829	amylase	-
68382	beta-galactosidase	-	3.2.1.23	from API zym
121829	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121829	caseinase	-	3.4.21.50
31295	catalase	+	1.11.1.6
121829	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
121829	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
121829	gamma-glutamyltransferase	-	2.3.2.2
121829	gelatinase	-
121829	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
121829	lipase	-
68382	lipase (C 14)	-		from API zym
121829	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121829	ornithine decarboxylase	-	4.1.1.17
121829	oxidase	-
121829	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	-	3.4.21.4	from API zym
121829	tryptophan deaminase	-
121829	tween esterase	-
121829	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121829	-	-	+	+	-	+	-	-	-	+	-	+	-	-	-	-	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
121829	not determinedn.d.	+/-	-	-	-	+/-	+/-	-	-	-	-	+/-	+/-	+/-	-	-	-	-	+/-	+/-	-	-	+/-	+/-	+/-	+/-	+/-	+/-	+/-	-	-	+/-	+/-	-	-	-	-	-	-	+/-	-	-	+/-	-	-	-	-	+/-	-	+/-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
121829	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Geographic location	Latitude	Longitude
6298	marine sediment (depth 4790m)	Pacific Ocean, Pacific Ocean, Ocean Drillin propgram, Nankai trough	32.245	135.025 32.245/135.025
67770	Deep sub-seafloor sediment core collected at Nankai Trough, off the coast of Japan
121829	Environment, Deep sub-seafloor sediment core	Nankai Trough

Taxonmaps

69479

Global distribution of 16S sequence AY485792 (>99% sequence identity) for Marinilactibacillus from Microbeatlas

Aquatic Samples	248
Soil Samples	153
Animal Samples	488
Plant Samples	52
Total Samples	941

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6298	1	Risk group (German classification) According to TRBA 466
121829	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	IMG-taxon 2636415975 annotated assembly for Marinilactibacillus piezotolerans DSM 16108	scaffold	GCA_900114235	258723.5	2636415975	258723	46.94

Genome browser: GCA_900114235

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
6298	Marinilactibacillus piezotolerans 16S ribosomal RNA gene, partial sequence	AY485792	1332	258723
124043	Marinilactibacillus piezotolerans gene for 16S rRNA, partial sequence.	AB247277	1510	258723
124043	Marinilactibacillus piezotolerans gene for 16S rRNA, partial sequence, strain: JCM 12337.	AB294178	1491	258723

GC content

@ref	GC-content (mol%)
31295	42
67770	42

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Marinilactibacillus piezotolerans DSM 16108
NCBI: GCA_900114235

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	57.60	no
125439	motility	BacteriaNetⓘ	yes	63.20	no
125439	gram_stain	BacteriaNetⓘ	variable	82.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Marinilactibacillus piezotolerans DSM 16108
NCBI: GCA_900114235.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.36	yes
125438	anaerobic	anaerobicⓘ	no	86.77	yes
125438	aerobic	aerobicⓘ	no	81.17	no
125438	spore-forming	spore-formingⓘ	no	77.06	yes
125438	thermophilic	thermophileⓘ	no	90.67	no
125438	flagellated	motile2+ⓘ	yes	65.86	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Culturable prokaryotic diversity of deep, gas hydrate sediments: first use of a continuous high-pressure, anaerobic, enrichment and isolation system for subseafloor sediments (DeepIsoBUG).	Parkes RJ, Sellek G, Webster G, Martin D, Anders E, Weightman AJ, Sass H.	Environ Microbiol	10.1111/j.1462-2920.2009.02018.x	2009
Phylogeny	Marinilactibacillus kalidii sp. nov., an Indole Acetic Acid-Producing Endophyte Isolated from a Shoot of Halophyte Kalidium cuspidatum.	Huang XX, Xu L, Shang J, Sun JQ	Curr Microbiol	10.1007/s00284-022-02894-6	2022
Phylogeny	Marinilactibacillus piezotolerans sp. nov., a novel marine lactic acid bacterium isolated from deep sub-seafloor sediment of the Nankai Trough.	Toffin L, Zink K, Kato C, Pignet P, Bidault A, Bienvenu N, Birrien JL, Prieur D	Int J Syst Evol Microbiol	10.1099/ijs.0.63236-0	2005

References

#6298	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16108
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#31295	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27614 (see below)
#34008	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121829	Collection of Institut Pasteur ; Curators of the CIP; CIP 108666
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Marinilactibacillus piezotolerans (DSM 16108, LT20, CIP 108666)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Enzymes

API zym

API 50CHac

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900114235

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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