Polynucleobacter acidiphobus (DSM 21994, CIP 110079, MWH-PoolGreenA3)

Name: Polynucleobacter acidiphobus (DSM 21994, CIP 110079, MWH-PoolGreenA3)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2079

Type strain

Strain Designation MWH-PoolGreenA3

Culture col. no. DSM 21994 CIP 110079 MWH-PoolGreenA3

NCBI tax ID(s) 556053

Special Collections DSMZ CIP Literature strains

History <- M. W. Hahn, Austrian Academy of Sci., Inst. Limnology; MWH-PoolGreenA3 CIP <- 2009, M. Hahn, Austrian Academy of Sciences, Inst. for Limnology, Mondsee, Austria: strain MWH-PoolGreenA3

Links

Polynucleobacter acidiphobus MWH-PoolGreenA3 is an aerobe, chemoorganotroph, rod-shaped bacterium that was isolated from freshwater from a rock pool.

rod-shaped aerobe chemoorganotroph genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 371502

CIP 110079,DSM 21994

@ref 20215

Last LPSN update 2026-02-09 11:18:14

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Polynucleobacter

Species Polynucleobacter acidiphobus

Full scientific name Polynucleobacter acidiphobus Hahn et al. 2011

BacDive ID	Other strains from **Polynucleobacter acidiphobus** (1)	Type strain
162199	P. acidiphobus JCM 18220, NBRC 107833

Morphology

Cell morphology

@ref	Cell length	Cell width	Cell shape	Motility	Gram stain
23164	0.5-1.4 µm	0.4-0.5 µm	rod-shaped
116004			rod-shaped		negative

Colony morphology

@ref	Colony shape	Medium used	Incubation period
16087			3-7 days
23164	circular	NSY and R2A

Pigmentation

23164

Productionno

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
16087	NSY-MEDIUM FOR POLYNUCLEOBACTER (DSMZ Medium 1167)	Medium recipe at MediaDive	Name: NSY-MEDIUM FOR POLYNUCLEOBACTER (DSMZ Medium 1167) Composition: Soy peptone 1.0 g/l Nutrient broth 1.0 g/l Yeast extract 1.0 g/l MgSO4 x 7 H2O 0.075 g/l Ca(NO3)2 x 4 H2O 0.043 g/l NaHCO3 0.016 g/l KCl 0.005 g/l K2HPO4 x 3 H2O 0.0037 g/l Na2-EDTA 5e-05 g/l H3BO3 3e-05 g/l CoCl2 x 6 H2O 2e-05 g/l FeSO4 x 7 H2O 2e-05 g/l ZnSO4 x 7 H2O 1e-05 g/l MnCl2 x 4 H2O 3e-06 g/l Na2MoO4 x 2 H2O 3e-06 g/l NiCl2 x 6 H2O 2e-06 g/l CuCl2 x 2 H2O 1e-06 g/l Tap water Distilled water
16087	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
23164	NSY and R2A
33656	MEDIUM718 - for Polynucleobacter rarum
116004	CIP Medium 751A	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
16087	positive	growth	25
23164	negative	growth	5
23164	positive	growth	35
33656	positive	growth	25

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
23164	aerobe
23164	facultative anaerobe

Nutrition type

23164

Typechemoorganotroph

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23164	NaCl	positive	maximum	0.3 %(w/v)
23164	NaCl	positive	growth	0 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23164	39150 ChEBI	4-oxopentanoate	-	carbon source
23164	39150 ChEBI	4-oxopentanoate	-	growth
23164	30089 ChEBI	acetate	+	carbon source
23164	30089 ChEBI	acetate	+	growth
23164	17750 ChEBI	betaine	-	carbon source
23164	17750 ChEBI	betaine	-	growth
23164	16947 ChEBI	citrate	-	carbon source
23164	16947 ChEBI	citrate	-	growth
23164	15824 ChEBI	D-fructose	-	carbon source
23164	15824 ChEBI	D-fructose	-	growth
23164	28847 ChEBI	D-fucose	-	carbon source
23164	28847 ChEBI	D-fucose	-	growth
23164	12936 ChEBI	D-galactose	-	carbon source
23164	12936 ChEBI	D-galactose	-	growth
23164	18024 ChEBI	D-galacturonic acid	-	carbon source
23164	18024 ChEBI	D-galacturonic acid	-	growth
23164	17634 ChEBI	D-glucose	+	carbon source
23164	17634 ChEBI	D-glucose	+	growth
23164	62318 ChEBI	D-lyxose	-	carbon source
23164	62318 ChEBI	D-lyxose	-	growth
23164	16024 ChEBI	D-mannose	-	carbon source
23164	16024 ChEBI	D-mannose	-	growth
23164	17924 ChEBI	D-sorbitol	-	carbon source
23164	17924 ChEBI	D-sorbitol	-	growth
23164	15740 ChEBI	formate	-	carbon source
23164	15740 ChEBI	formate	-	growth
23164	29806 ChEBI	fumarate	-	carbon source
23164	29806 ChEBI	fumarate	-	growth
23164	29805 ChEBI	glycolate	-	carbon source
23164	29805 ChEBI	glycolate	-	growth
23164	36655 ChEBI	glyoxylate	-	carbon source
23164	36655 ChEBI	glyoxylate	-	growth
23164	16977 ChEBI	L-alanine	-	carbon source
23164	16977 ChEBI	L-alanine	-	growth
23164	17196 ChEBI	L-asparagine	-	carbon source
23164	17196 ChEBI	L-asparagine	-	growth
23164	29991 ChEBI	L-aspartate	-	carbon source
23164	29991 ChEBI	L-aspartate	-	growth
23164	17561 ChEBI	L-cysteine	+	carbon source
23164	17561 ChEBI	L-cysteine	+	growth
23164	29985 ChEBI	L-glutamate	-	carbon source
23164	29985 ChEBI	L-glutamate	-	growth
23164	17115 ChEBI	L-serine	-	carbon source
23164	25115 ChEBI	malate	+	carbon source
23164	25115 ChEBI	malate	+	growth
23164	15792 ChEBI	malonate	+	carbon source
23164	15792 ChEBI	malonate	+	growth
23164	30623 ChEBI	oxalate	-	carbon source
23164	30623 ChEBI	oxalate	-	growth
23164	16452 ChEBI	oxaloacetate	+	carbon source
23164	16452 ChEBI	oxaloacetate	+	growth
23164	17272 ChEBI	propionate	-	carbon source
23164	17272 ChEBI	propionate	-	growth
23164	15361 ChEBI	pyruvate	+	carbon source
23164	30031 ChEBI	succinate	+	carbon source
23164	30031 ChEBI	succinate	+	growth
23164	16094 ChEBI	thiosulfate	-	growth
23164	16199 ChEBI	urea	-	growth

Enzymes

@ref	Value	Activity	Ec
23164	catalase	+	1.11.1.6
23164	cytochrome oxidase	+	1.9.3.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Pond (small)
#Environmental	#Terrestrial	#Geologic

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Isolation date
16087	freshwater from a rock pool	Corsica, 42° 10' 21.05'' N 8° 53' 54.54''E	France	FRA	Europe	42.1725	8.8983 42.1725/8.8983
116004	Environment, Freshwater, rock pool	Corsica	France	FRA	Europe			2005

Taxonmaps

69479

Global distribution of 16S sequence FM208180 (>99% sequence identity) for Polynucleobacter acidiphobus from Microbeatlas

Aquatic Samples	1532
Soil Samples	7
Animal Samples	34
Plant Samples	4
Total Samples	1577

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16087	1	Risk group (German classification) According to TRBA 466
116004	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM306538v1 assembly for Polynucleobacter acidiphobus MWH-PoolGreenA3	chromosome	GCA_003065385	556053.3	2808606701	556053	78.94

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
16087	Polynucleobacter acidiphobus 16S rRNA gene (partial), IGS and 23S rRNA gene (partial), strain MWH-PoolGreenA3	FM208180	2082		556053

GC content

@ref	GC-content (mol%)	Method
16087	48.3	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Polynucleobacter acidiphobus sp. nov., a representative of an abundant group of planktonic freshwater bacteria.	Hahn MW, Lang E, Brandt U, Sproer C	Int J Syst Evol Microbiol	10.1099/ijs.0.023929-0	2010

References

#16087	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21994
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23164	Martin W. Hahn, Elke Lang, Ulrike Brandt, Cathrin Spröer: Polynucleobacter acidiphobus sp. nov., a representative of an abundant group of planktonic freshwater bacteria. IJSEM 61: 788 - 794 2011 ( DOI 10.1099/ijs.0.023929-0 , PubMed 20435747 )
#33656	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#116004	Collection of Institut Pasteur ; Curators of the CIP; CIP 110079
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Polynucleobacter acidiphobus (DSM 21994, CIP 110079, MWH-PoolGreenA3)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Nutrition type

Halophily

Metabolite utilization

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003065385

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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