Name: Polynucleobacter acidiphobus MWH-PoolGreenA3
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 2079

Type strain:

Species: Polynucleobacter acidiphobus

Strain Designation: MWH-PoolGreenA3

Culture col. no.: DSM 21994, CIP 110079

Strain history: CIP <- 2009, M. Hahn, Austrian Academy of Sciences, Inst. for Limnology, Mondsee, Austria: strain MWH-PoolGreenA3

NCBI tax ID(s): 556053 (species)

SI-ID 371502

CIP 110079, DSM 21994

Special Collections

Other strains from Polynucleobacter acidiphobus

Section

Polynucleobacter acidiphobus MWH-PoolGreenA3 is an aerobe, chemoorganotroph, rod-shaped bacterium that was isolated from freshwater from a rock pool.

rod-shaped
aerobe
chemoorganotroph
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Polynucleobacter*
	Species	**Polynucleobacter acidiphobus**
	Full Scientific Name (LPSN)	Polynucleobacter acidiphobus Hahn et al. 2011

Information on morphological and physiological properties Morphology

[Ref.: #116004]	Gram stain	negative

[Ref.: #116004]	Cell shape	rod-shaped
[Ref.: #23164]	Cell shape	rod-shaped

[Ref.: #116004]	Motility	no
[Ref.: #23164]	Motility	no

[Ref.: #23164]	Cell length	0.5-1.4 µm

[Ref.: #23164]	Cell width	0.4-0.5 µm

[Ref.: #23164]	Colony shape	circular
[Ref.: #23164]	Cultivation medium used	NSY and R2A

[Ref.: #16087]	Incubation period	3-7 days

[Ref.: #23164]	Pigment production	no
[Ref.: #23164]	Pigment name	no pigments

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16087]

Culture medium

NSY-MEDIUM FOR POLYNUCLEOBACTER (DSMZ Medium 1167)

[Ref.: #16087]

Culture medium growth

[Ref.: #16087]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16087]

Culture medium composition

expand / minimize

[Ref.: #16087]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #16087]

Culture medium growth

[Ref.: #16087]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16087]

Culture medium composition

expand / minimize

[Ref.: #23164]

Culture medium

NSY and R2A

[Ref.: #23164]

Culture medium growth

[Ref.: #33656]

Culture medium

MEDIUM718 - for Polynucleobacter rarum

[Ref.: #33656]

Culture medium growth

[Ref.: #116004]

Culture medium

CIP Medium 751A

[Ref.: #116004]

Culture medium growth

[Ref.: #116004]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #16087]		growth	25 °C
[Ref.: #23164]	no	growth	5 °C
[Ref.: #23164]		growth	35 °C
[Ref.: #33656]		growth	25 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23164]	Oxygen tolerance	aerobe
[Ref.: #23164]	Oxygen tolerance	facultative anaerobe

[Ref.: #23164]

Nutrition type

chemoorganotroph

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23164]		NaCl		maximum	0.3	%(w/v)
[Ref.: #23164]		NaCl		growth	0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23164]	4-oxopentanoate ChEBI	-	carbon source
[Ref.: #23164]	4-oxopentanoate ChEBI	-	growth
[Ref.: #23164]	acetate ChEBI	+	carbon source
[Ref.: #23164]	acetate ChEBI	+	growth
[Ref.: #23164]	betaine ChEBI	-	carbon source
[Ref.: #23164]	betaine ChEBI	-	growth
[Ref.: #23164]	citrate ChEBI	-	carbon source
[Ref.: #23164]	citrate ChEBI	-	growth
[Ref.: #23164]	D-fructose ChEBI	-	carbon source
[Ref.: #23164]	D-fructose ChEBI	-	growth
[Ref.: #23164]	D-fucose ChEBI	-	carbon source
[Ref.: #23164]	D-fucose ChEBI	-	growth
[Ref.: #23164]	D-galactose ChEBI	-	carbon source
[Ref.: #23164]	D-galactose ChEBI	-	growth
[Ref.: #23164]	D-galacturonic acid ChEBI	-	carbon source
[Ref.: #23164]	D-galacturonic acid ChEBI	-	growth
[Ref.: #23164]	D-glucose ChEBI	+	carbon source
[Ref.: #23164]	D-glucose ChEBI	+	growth
[Ref.: #23164]	D-lyxose ChEBI	-	carbon source
[Ref.: #23164]	D-lyxose ChEBI	-	growth
[Ref.: #23164]	D-mannose ChEBI	-	carbon source
[Ref.: #23164]	D-mannose ChEBI	-	growth
[Ref.: #23164]	D-sorbitol ChEBI	-	carbon source
[Ref.: #23164]	D-sorbitol ChEBI	-	growth
[Ref.: #23164]	formate ChEBI	-	carbon source
[Ref.: #23164]	formate ChEBI	-	growth
[Ref.: #23164]	fumarate ChEBI	-	carbon source
[Ref.: #23164]	fumarate ChEBI	-	growth
[Ref.: #23164]	glycolate ChEBI	-	carbon source
[Ref.: #23164]	glycolate ChEBI	-	growth
[Ref.: #23164]	glyoxylate ChEBI	-	carbon source
[Ref.: #23164]	glyoxylate ChEBI	-	growth
[Ref.: #23164]	L-alanine ChEBI	-	carbon source
[Ref.: #23164]	L-alanine ChEBI	-	growth
[Ref.: #23164]	L-asparagine ChEBI	-	carbon source
[Ref.: #23164]	L-asparagine ChEBI	-	growth
[Ref.: #23164]	L-aspartate ChEBI	-	carbon source
[Ref.: #23164]	L-aspartate ChEBI	-	growth
[Ref.: #23164]	L-cysteine ChEBI	+	carbon source
[Ref.: #23164]	L-cysteine ChEBI	+	growth
[Ref.: #23164]	L-glutamate ChEBI	-	carbon source
[Ref.: #23164]	L-glutamate ChEBI	-	growth
[Ref.: #23164]	L-serine ChEBI	-	carbon source
[Ref.: #23164]	malate ChEBI	+	carbon source
[Ref.: #23164]	malate ChEBI	+	growth
[Ref.: #23164]	malonate ChEBI	+	carbon source
[Ref.: #23164]	malonate ChEBI	+	growth
[Ref.: #23164]	oxalate ChEBI	-	carbon source
[Ref.: #23164]	oxalate ChEBI	-	growth
[Ref.: #23164]	oxaloacetate ChEBI	+	carbon source
[Ref.: #23164]	oxaloacetate ChEBI	+	growth
[Ref.: #23164]	propionate ChEBI	-	carbon source
[Ref.: #23164]	propionate ChEBI	-	growth
[Ref.: #23164]	pyruvate ChEBI	+	carbon source
[Ref.: #23164]	succinate ChEBI	+	carbon source
[Ref.: #23164]	succinate ChEBI	+	growth
[Ref.: #23164]	thiosulfate ChEBI	-	growth
[Ref.: #23164]	urea ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #23164]		catalase	+	1.11.1.6
[Ref.: #23164]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16087]	Sample type/isolated from	freshwater from a rock pool
[Ref.: #16087]	Geographic location (country and/or sea, region)	Corsica, 42° 10' 21.05'' N 8° 53' 54.54''E
[Ref.: #16087]	Country	France
[Ref.: #16087]	Country ISO 3 Code	FRA
[Ref.: #16087]	Continent	Europe
[Ref.: #16087]	Geographic location	42.1725°/8.8983°

[Ref.: #116004]	Sample type/isolated from	Environment, Freshwater, rock pool
[Ref.: #116004]	Geographic location (country and/or sea, region)	Corsica
[Ref.: #116004]	Country	France
[Ref.: #116004]	Country ISO 3 Code	FRA
[Ref.: #116004]	Continent	Europe
[Ref.: #116004]	Isolation date	2005
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Pond (small)
#Environmental	#Terrestrial	#Geologic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FM208180 (>99% sequence identity) for Polynucleobacter acidiphobus from Microbeatlas

Aquatic Samples	1532
Soil Samples	7
Animal Samples	34
Plant Samples	4
Total Samples	1577

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16087]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116004]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16087]	Polynucleobacter acidiphobus 16S rRNA gene (partial), IGS and 23S rRNA gene (partial), strain MWH-PoolGreenA3	FM208180	2082	GenBank ENA	556053 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Polynucleobacter acidiphobus MWH-PoolGreenA3	GCA_003065385	chromosome	GenBank ENA	556053 tax ID	*
[Ref.: #66792]	Polynucleobacter acidiphobus MWH-PoolGreenA3	2808606701	complete	JGI IMG/M	556053 tax ID	*

[Ref.: #16087]

GC-content

48.3 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #16087]

Culture collection no.

DSM 21994, CIP 110079

[Ref.: #71726]

SI-ID 371502

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Polynucleobacter acidiphobus sp. nov., a representative of an abundant group of planktonic freshwater bacteria.	Hahn MW, Lang E, Brandt U, Sproer C	Int J Syst Evol Microbiol	10.1099/ijs.0.023929-0	2010	*

References

#16087

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21994

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23164

Martin W. Hahn, Elke Lang, Ulrike Brandt, Cathrin Spröer: Polynucleobacter acidiphobus sp. nov., a representative of an abundant group of planktonic freshwater bacteria. IJSEM 61: 788 - 794 2011 ( DOI 10.1099/ijs.0.023929-0 , PubMed 20435747 )

#33656 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71726

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID371502.1 )

#116004 Collection of Institut Pasteur ; Curators of the CIP; CIP 110079
* These data were automatically processed and therefore are not curated

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