Proteiniborus ethanoligenes (DSM 21650, CGMCC 1.5055, JCM 14574)

Name: Proteiniborus ethanoligenes (DSM 21650, CGMCC 1.5055, JCM 14574)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 18078

Type strain

Strain Designation GW

Culture col. no. DSM 21650 CGMCC 1.5055 JCM 14574 GW

NCBI tax ID(s) 1123007 415015

Special Collections DSMZ JCM Literature strains

Links

Proteiniborus ethanoligenes GW is an anaerobe, mesophilic, Gram-positive prokaryote that produces alcohol and was isolated from mesophilic hydrogen-producing granular sludge used to treat food industry wastewater.

alcohol production Gram-positive rod-shaped anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 323989

CGMCC 1.5055,JCM 14574,DSM 21650

@ref 20215

Last LPSN update 2025-12-16 09:51:15

Domain Bacillati

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Clostridiaceae

Genus Proteiniborus

Species Proteiniborus ethanoligenes

Full scientific name Proteiniborus ethanoligenes Niu et al. 2008

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
32198	positive	2.6 µm	0.55 µm	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
15866	FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a)		Medium recipe at MediaDive	Name: FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a) Composition: Fastidious Anaerobe Basal Broth 35.4 g/l Distilled water
15866	CHOPPED MEAT MEDIUM (DSMZ Medium 78)		Medium recipe at MediaDive	Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
15866	positive	growth	37	mesophilic
32198	positive	growth	20-48
32198	positive	optimum	37	mesophilic
67770	positive	growth	37	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
32198	positive	optimum	8.65
32198	positive	growth	6.4-10	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
15866	anaerobe
32198	anaerobe
125439	obligate anaerobe	90

Spore formation

32198

Spore formationno

Compound production

15866

Compoundethanol

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
32198	17632 ChEBI	nitrate	+	reduction

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	adipate degradation	100	2 of 2
66794	cardiolipin biosynthesis	100	7 of 7
66794	coenzyme A metabolism	100	4 of 4
66794	isoleucine metabolism	100	8 of 8
66794	teichoic acid biosynthesis	100	1 of 1
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	palmitate biosynthesis	95.45	21 of 22
66794	threonine metabolism	90	9 of 10
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	propanol degradation	85.71	6 of 7
66794	phenylalanine metabolism	84.62	11 of 13
66794	NAD metabolism	83.33	15 of 18
66794	vitamin B12 metabolism	82.35	28 of 34
66794	pyrimidine metabolism	82.22	37 of 45
66794	peptidoglycan biosynthesis	80	12 of 15
66794	cellulose degradation	80	4 of 5
66794	glycine betaine biosynthesis	80	4 of 5
66794	lipoate biosynthesis	80	4 of 5
66794	phenylacetate degradation (aerobic)	80	4 of 5
66794	purine metabolism	78.72	74 of 94
66794	photosynthesis	78.57	11 of 14
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	glycolysis	76.47	13 of 17
66794	alanine metabolism	75.86	22 of 29
66794	glycogen biosynthesis	75	3 of 4
66794	ppGpp biosynthesis	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	biotin biosynthesis	75	3 of 4
66794	reductive acetyl coenzyme A pathway	71.43	5 of 7
66794	propionate fermentation	70	7 of 10
66794	glutamate and glutamine metabolism	67.86	19 of 28
66794	acetoin degradation	66.67	2 of 3
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	octane oxidation	66.67	2 of 3
66794	oxidative phosphorylation	64.84	59 of 91
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	gluconeogenesis	62.5	5 of 8
66794	cysteine metabolism	61.11	11 of 18
66794	hydrogen production	60	3 of 5
66794	factor 420 biosynthesis	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	methylglyoxal degradation	60	3 of 5
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	ubiquinone biosynthesis	57.14	4 of 7
66794	serine metabolism	55.56	5 of 9
66794	d-mannose degradation	55.56	5 of 9
66794	d-xylose degradation	54.55	6 of 11
66794	urea cycle	53.85	7 of 13
66794	lysine metabolism	50	21 of 42
66794	citric acid cycle	50	7 of 14
66794	ethanol fermentation	50	1 of 2
66794	sulfopterin metabolism	50	2 of 4
66794	degradation of sugar alcohols	50	8 of 16
66794	CMP-KDO biosynthesis	50	2 of 4
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	non-pathway related	50	19 of 38
66794	ribulose monophosphate pathway	50	1 of 2
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	ketogluconate metabolism	50	4 of 8
66794	phenylmercury acetate degradation	50	1 of 2
66794	histidine metabolism	48.28	14 of 29
66794	tryptophan metabolism	47.37	18 of 38
66794	vitamin B1 metabolism	46.15	6 of 13
66794	pentose phosphate pathway	45.45	5 of 11
66794	lipid metabolism	45.16	14 of 31
66794	tetrahydrofolate metabolism	42.86	6 of 14
66794	heme metabolism	42.86	6 of 14
66794	methionine metabolism	42.31	11 of 26
66794	gallate degradation	40	2 of 5
66794	glycogen metabolism	40	2 of 5
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	flavin biosynthesis	40	6 of 15
66794	glycine metabolism	40	4 of 10
66794	starch degradation	40	4 of 10
66794	polyamine pathway	39.13	9 of 23
66794	degradation of hexoses	38.89	7 of 18
66794	leucine metabolism	38.46	5 of 13
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	arginine metabolism	37.5	9 of 24
66794	carnitine metabolism	37.5	3 of 8
66794	ascorbate metabolism	36.36	8 of 22
66794	3-phenylpropionate degradation	33.33	5 of 15
66794	sulfoquinovose degradation	33.33	1 of 3
66794	enterobactin biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	lipid A biosynthesis	33.33	3 of 9
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	nitrate assimilation	33.33	3 of 9
66794	4-hydroxymandelate degradation	33.33	3 of 9
66794	degradation of pentoses	32.14	9 of 28
66794	sulfate reduction	30.77	4 of 13
66794	Entner Doudoroff pathway	30	3 of 10
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	tyrosine metabolism	28.57	4 of 14
66794	glutathione metabolism	28.57	4 of 14
66794	metabolism of disaccharids	27.27	3 of 11
66794	phenol degradation	25	5 of 20
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	vitamin E metabolism	25	1 of 4
66794	androgen and estrogen metabolism	25	4 of 16
66794	dTDPLrhamnose biosynthesis	25	2 of 8
66794	phosphatidylethanolamine bioynthesis	23.08	3 of 13

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	-
#Engineered	#Waste	#Industrial wastewater
#Engineered	#Waste	#Activated sludge

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
15866	mesophilic hydrogen-producing granular sludge used to treat food industry wastewater	China	CHN	Asia
67770	Mesophilic hydrogen-producing granular sludge used to treat food industry wastewater

Taxonmaps

69479

Global distribution of 16S sequence EF116488 (>99% sequence identity) for Proteiniborus ethanoligenes subclade from Microbeatlas

Aquatic Samples	627
Soil Samples	182
Animal Samples	367
Plant Samples	53
Total Samples	1229

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15866	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2599185206 annotated assembly for Proteiniborus ethanoligenes DSM 21650	scaffold	GCA_900107485	415015.4	2599185206	415015	64.09

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15866	Proteiniborus ethanoligenes strain GW 16S ribosomal RNA gene, partial sequence	EF116488	1523		415015

GC content

@ref	GC-content (mol%)	Method
15866	38	thermal denaturation, midpoint method (Tm)
32198	38

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Proteiniborus ethanoligenes DSM 21650
NCBI: GCA_900107485

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	61.90	no
125439	motility	BacteriaNetⓘ	yes	63.00	no
125439	gram_stain	BacteriaNetⓘ	variable	70.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	90.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Proteiniborus ethanoligenes strain DSM 21650
PATRIC: 415015.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	56.95	no
125438	anaerobic	anaerobicⓘ	yes	88.74	no
125438	spore-forming	spore-formingⓘ	yes	55.15	yes
125438	aerobic	aerobicⓘ	no	95.10	no
125438	thermophilic	thermophileⓘ	no	75.67	no
125438	flagellated	motile2+ⓘ	yes	78.13	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Detailed description of Senegalia massiliensis strain SIT17^T , a bacterium isolated from the human gut.	Traore SI, Ngom II, Lo CI, Di Pinto F, Sokhna C, Fournier PE, Raoult D, Fenollar F.	New Microbes New Infect	10.1016/j.nmni.2020.100700	2020
Genetics	A Comparative Analysis of Biosynthetic Gene Clusters in Lean and Obese Humans.	Wang S, Li N, Li N, Zou H, Wu M.	Biomed Res Int	10.1155/2019/6361320	2019
Phylogeny	Proteiniborus ethanoligenes gen. nov., sp. nov., an anaerobic protein-utilizing bacterium.	Niu L, Song L, Dong X	Int J Syst Evol Microbiol	10.1099/ijs.0.65108-0	2008

References

#15866	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21650
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28440	IJSEM 12 2008 ( DOI 10.1099/ijs.0.65108-0 , PubMed 18175674 )
#32198	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28440
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Proteiniborus ethanoligenes (DSM 21650, CGMCC 1.5055, JCM 14574)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Metabolite utilization

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900107485

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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