Name: Proteiniborus ethanoligenes GW
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 18078

Type strain:

Species: Proteiniborus ethanoligenes

Strain Designation: GW

Culture col. no.: DSM 21650, CGMCC 1.5055, JCM 14574

Strain history: X. Dong strain GW.

NCBI tax ID(s): 1123007 (strain), 415015 (species)

SI-ID 323989

CGMCC 1.5055, JCM 14574, DSM 21650

Section

Proteiniborus ethanoligenes GW is an anaerobe, mesophilic, Gram-positive bacterium that produces alcohol and was isolated from mesophilic hydrogen-producing granular sludge used to treat food industry wastewater.

alcohol production
Gram-positive
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Proteiniborus*
	Species	**Proteiniborus ethanoligenes**
	Full Scientific Name (LPSN)	Proteiniborus ethanoligenes Niu et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #32198]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #32198]	Cell length	2.6 µm

[Ref.: #32198]	Cell width	0.55 µm

[Ref.: #32198]	Cell shape	rod-shaped

[Ref.: #32198]	Motility	no
[Ref.: #69480]	Motility	yes Confidence in %95.203

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15866]

Culture medium

FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a)

[Ref.: #15866]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

CHOPPED MEAT MEDIUM (DSMZ Medium 78)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #15866]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #15866]	growth	37 °C
[Ref.: #32198]	growth	20-48 °C
[Ref.: #32198]	optimum	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #15866]	Temperature range	mesophilic
[Ref.: #32198]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #32198]			optimum	8.65
[Ref.: #32198]			growth	6.4-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #15866]	Oxygen tolerance	anaerobe
[Ref.: #32198]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %100

[Ref.: #32198]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.42

[Ref.: #15866]

Name of produced compound

ethanol

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32198]		nitrate ChEBI	+	reduction

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	isoleucine metabolism	100	8 of 8
[Ref.: #66794]	teichoic acid biosynthesis	100	1 of 1
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	palmitate biosynthesis	95.45	21 of 22
[Ref.: #66794]	threonine metabolism	90	9 of 10
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	valine metabolism	88.89	8 of 9
[Ref.: #66794]	propanol degradation	85.71	6 of 7
[Ref.: #66794]	phenylalanine metabolism	84.62	11 of 13
[Ref.: #66794]	NAD metabolism	83.33	15 of 18
[Ref.: #66794]	vitamin B12 metabolism	82.35	28 of 34
[Ref.: #66794]	pyrimidine metabolism	82.22	37 of 45
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	glycine betaine biosynthesis	80	4 of 5
[Ref.: #66794]	lipoate biosynthesis	80	4 of 5
[Ref.: #66794]	phenylacetate degradation (aerobic)	80	4 of 5
[Ref.: #66794]	purine metabolism	78.72	74 of 94
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	CO2 fixation in Crenarchaeota	77.78	7 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	77.78	7 of 9
[Ref.: #66794]	glycolysis	76.47	13 of 17
[Ref.: #66794]	alanine metabolism	75.86	22 of 29
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	butanoate fermentation	75	3 of 4
[Ref.: #66794]	C4 and CAM-carbon fixation	75	6 of 8
[Ref.: #66794]	biotin biosynthesis	75	3 of 4
[Ref.: #66794]	reductive acetyl coenzyme A pathway	71.43	5 of 7
[Ref.: #66794]	propionate fermentation	70	7 of 10
[Ref.: #66794]	glutamate and glutamine metabolism	67.86	19 of 28
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	cyanate degradation	66.67	2 of 3
[Ref.: #66794]	glycolate and glyoxylate degradation	66.67	4 of 6
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	oxidative phosphorylation	64.84	59 of 91
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	gluconeogenesis	62.5	5 of 8
[Ref.: #66794]	cysteine metabolism	61.11	11 of 18
[Ref.: #66794]	hydrogen production	60	3 of 5
[Ref.: #66794]	factor 420 biosynthesis	60	3 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	methylglyoxal degradation	60	3 of 5
[Ref.: #66794]	isoprenoid biosynthesis	57.69	15 of 26
[Ref.: #66794]	ubiquinone biosynthesis	57.14	4 of 7
[Ref.: #66794]	serine metabolism	55.56	5 of 9
[Ref.: #66794]	d-mannose degradation	55.56	5 of 9
[Ref.: #66794]	d-xylose degradation	54.55	6 of 11
[Ref.: #66794]	urea cycle	53.85	7 of 13
[Ref.: #66794]	lysine metabolism	50	21 of 42
[Ref.: #66794]	citric acid cycle	50	7 of 14
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	sulfopterin metabolism	50	2 of 4
[Ref.: #66794]	degradation of sugar alcohols	50	8 of 16
[Ref.: #66794]	CMP-KDO biosynthesis	50	2 of 4
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	non-pathway related	50	19 of 38
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	50	6 of 12
[Ref.: #66794]	ketogluconate metabolism	50	4 of 8
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	histidine metabolism	48.28	14 of 29
[Ref.: #66794]	tryptophan metabolism	47.37	18 of 38
[Ref.: #66794]	vitamin B1 metabolism	46.15	6 of 13
[Ref.: #66794]	pentose phosphate pathway	45.45	5 of 11
[Ref.: #66794]	lipid metabolism	45.16	14 of 31
[Ref.: #66794]	tetrahydrofolate metabolism	42.86	6 of 14
[Ref.: #66794]	heme metabolism	42.86	6 of 14
[Ref.: #66794]	methionine metabolism	42.31	11 of 26
[Ref.: #66794]	gallate degradation	40	2 of 5
[Ref.: #66794]	glycogen metabolism	40	2 of 5
[Ref.: #66794]	ethylmalonyl-CoA pathway	40	2 of 5
[Ref.: #66794]	flavin biosynthesis	40	6 of 15
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	starch degradation	40	4 of 10
[Ref.: #66794]	polyamine pathway	39.13	9 of 23
[Ref.: #66794]	degradation of hexoses	38.89	7 of 18
[Ref.: #66794]	leucine metabolism	38.46	5 of 13
[Ref.: #66794]	phenylpropanoid biosynthesis	38.46	5 of 13
[Ref.: #66794]	arginine metabolism	37.5	9 of 24
[Ref.: #66794]	carnitine metabolism	37.5	3 of 8
[Ref.: #66794]	ascorbate metabolism	36.36	8 of 22
[Ref.: #66794]	3-phenylpropionate degradation	33.33	5 of 15
[Ref.: #66794]	sulfoquinovose degradation	33.33	1 of 3
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	4-hydroxymandelate degradation	33.33	3 of 9
[Ref.: #66794]	degradation of pentoses	32.14	9 of 28
[Ref.: #66794]	sulfate reduction	30.77	4 of 13
[Ref.: #66794]	Entner Doudoroff pathway	30	3 of 10
[Ref.: #66794]	benzoyl-CoA degradation	28.57	2 of 7
[Ref.: #66794]	tyrosine metabolism	28.57	4 of 14
[Ref.: #66794]	glutathione metabolism	28.57	4 of 14
[Ref.: #66794]	metabolism of disaccharids	27.27	3 of 11
[Ref.: #66794]	phenol degradation	25	5 of 20
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	vitamin E metabolism	25	1 of 4
[Ref.: #66794]	androgen and estrogen metabolism	25	4 of 16
[Ref.: #66794]	dTDPLrhamnose biosynthesis	25	2 of 8
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	23.08	3 of 13
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15866]	Sample type/isolated from	mesophilic hydrogen-producing granular sludge used to treat food industry wastewater
[Ref.: #15866]	Country	China
[Ref.: #15866]	Country ISO 3 Code	CHN
[Ref.: #15866]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Mesophilic hydrogen-producing granular sludge used to treat food industry wastewater
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	-
#Engineered	#Waste	#Industrial wastewater
#Engineered	#Waste	#Activated sludge

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EF116488 (>99% sequence identity) for Proteiniborus ethanoligenes subclade from Microbeatlas

Aquatic Samples	627
Soil Samples	182
Animal Samples	367
Plant Samples	53
Total Samples	1229

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15866]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15866]	Proteiniborus ethanoligenes strain GW 16S ribosomal RNA gene, partial sequence	EF116488	1523	ENA	415015 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Proteiniborus ethanoligenes DSM 21650	GCA_900107485	scaffold	GenBank	415015 tax ID
[Ref.: #66792]	Proteiniborus ethanoligenes strain DSM 21650	415015.4	wgs	PATRIC	415015 tax ID	*
[Ref.: #66792]	Proteiniborus ethanoligenes DSM 21650	2599185206	draft	JGI IMG/M	415015 tax ID	*

[Ref.: #15866]	GC-content	38 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #32198]	GC-content	38 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Proteiniborus ethanoligenes DSM 21650 NCBI: GCA_900107485.1
[Ref.: #69481]	spore-forming	no	80	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Proteiniborus ethanoligenes strain DSM 21650 PATRIC: 415015.4
[Ref.: #69480]	gram-positive	yes	79.909	yes
[Ref.: #69480]	anaerobic	yes	98.645	yes
[Ref.: #69480]	halophile	no	89.328	no
[Ref.: #69480]	spore-forming	yes	79.372	yes
[Ref.: #69480]	glucose-util	yes	77.951	no
[Ref.: #69480]	aerobic	no	96.148	yes
[Ref.: #69480]	flagellated	no	83.736	yes
[Ref.: #69480]	thermophile	no	95.547	yes
[Ref.: #69480]	motile	yes	80.911	yes
[Ref.: #69480]	glucose-ferment	no	52.257	no

Availability in culture collections External links

[Ref.: #15866]

Culture collection no.

DSM 21650, CGMCC 1.5055, JCM 14574

[Ref.: #87066]

SI-ID 323989

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Proteiniborus ethanoligenes gen. nov., sp. nov., an anaerobic protein-utilizing bacterium.	Niu L, Song L, Dong X	Int J Syst Evol Microbiol	10.1099/ijs.0.65108-0	2008	*

References

#15866

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21650

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32198

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28440 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87066

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID323989.1 )

#28440

IJSEM 12 2008 ( DOI 10.1099/ijs.0.65108-0 , PubMed 18175674 )

* These data were automatically processed and therefore are not curated

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Proteiniborus ethanoligenes DSM 21650

Proteiniborus ethanoligenes strain DSM 21650

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