Name: Mahella australiensis 50-1 BON
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17993

Type strain:

Species: Mahella australiensis

Strain Designation: 50-1 BON

Culture col. no.: DSM 15567, CIP 107919

Strain history: <- M.-L. Fardeau, Universités de Provence et de la Méditerranée, ESIL, Marseille, France; 50-1 BON <- M. B. Salinas {2002}

NCBI tax ID(s): 697281 (strain), 252966 (species)

SI-ID 134438

CIP 107919, DSM 15567

Section

Mahella australiensis 50-1 BON is an anaerobe, spore-forming, thermophilic bacterium that was isolated from oil sample.

Gram-positive
motile
rod-shaped
spore-forming
anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Thermoanaerobacterales*
	Family	"Mahellaceae"
	Genus	*Mahella*
	Species	**Mahella australiensis**
	Full Scientific Name (LPSN)	Mahella australiensis Bonilla Salinas et al. 2004

Information on morphological and physiological properties Morphology

[Ref.: #30092]	Gram stain	positive

[Ref.: #30092]	Cell length	11.5 µm

[Ref.: #30092]	Cell width	0.5 µm

[Ref.: #30092]	Cell shape	rod-shaped

[Ref.: #30092]	Motility	yes

Multimedia content

[Ref.: #66793]

Caption:

electron microscopic image

[Ref.: #66793]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6004]

Culture medium

WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2) (DSMZ Medium 339a)

[Ref.: #6004]

Culture medium growth

[Ref.: #6004]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6004]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #6004]	growth	50 °C
[Ref.: #30092]	growth	30-60 °C
[Ref.: #30092]	optimum	50 °C

[Ref.: #6004]	Temperature range	thermophilic
[Ref.: #30092]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #30092]			optimum	7.5
[Ref.: #30092]			growth	5.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #6004]	Oxygen tolerance	anaerobe
[Ref.: #30092]	Oxygen tolerance	anaerobe

[Ref.: #30092]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30092]		NaCl		growth	0-4	%
[Ref.: #30092]		NaCl		optimum	0.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30092]	arabinose ChEBI	+	carbon source
[Ref.: #30092]	cellobiose ChEBI	+	carbon source
[Ref.: #30092]	fructose ChEBI	+	carbon source
[Ref.: #30092]	galactose ChEBI	+	carbon source
[Ref.: #30092]	glucose ChEBI	+	carbon source
[Ref.: #30092]	mannose ChEBI	+	carbon source
[Ref.: #30092]	sucrose ChEBI	+	carbon source
[Ref.: #30092]	xylose ChEBI	+	carbon source

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	ribulose monophosphate pathway	100	2 of 2
[Ref.: #66794]	aminopropanol phosphate biosynthesis	100	2 of 2
[Ref.: #66794]	glycogen metabolism	100	5 of 5
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	palmitate biosynthesis	95.45	21 of 22
[Ref.: #66794]	threonine metabolism	90	9 of 10
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	valine metabolism	88.89	8 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	d-mannose degradation	88.89	8 of 9
[Ref.: #66794]	isoleucine metabolism	87.5	7 of 8
[Ref.: #66794]	glycolate and glyoxylate degradation	83.33	5 of 6
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	hydrogen production	80	4 of 5
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	Entner Doudoroff pathway	80	8 of 10
[Ref.: #66794]	glycine betaine biosynthesis	80	4 of 5
[Ref.: #66794]	vitamin B12 metabolism	79.41	27 of 34
[Ref.: #66794]	NAD metabolism	77.78	14 of 18
[Ref.: #66794]	CO2 fixation in Crenarchaeota	77.78	7 of 9
[Ref.: #66794]	serine metabolism	77.78	7 of 9
[Ref.: #66794]	phenylalanine metabolism	76.92	10 of 13
[Ref.: #66794]	vitamin B1 metabolism	76.92	10 of 13
[Ref.: #66794]	glycolysis	76.47	13 of 17
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	ketogluconate metabolism	75	6 of 8
[Ref.: #66794]	butanoate fermentation	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	biotin biosynthesis	75	3 of 4
[Ref.: #66794]	degradation of sugar alcohols	75	12 of 16
[Ref.: #66794]	gluconeogenesis	75	6 of 8
[Ref.: #66794]	C4 and CAM-carbon fixation	75	6 of 8
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	purine metabolism	72.34	68 of 94
[Ref.: #66794]	heme metabolism	71.43	10 of 14
[Ref.: #66794]	reductive acetyl coenzyme A pathway	71.43	5 of 7
[Ref.: #66794]	photosynthesis	71.43	10 of 14
[Ref.: #66794]	ubiquinone biosynthesis	71.43	5 of 7
[Ref.: #66794]	propanol degradation	71.43	5 of 7
[Ref.: #66794]	pyrimidine metabolism	71.11	32 of 45
[Ref.: #66794]	myo-inositol biosynthesis	70	7 of 10
[Ref.: #66794]	starch degradation	70	7 of 10
[Ref.: #66794]	degradation of sugar acids	68	17 of 25
[Ref.: #66794]	degradation of pentoses	67.86	19 of 28
[Ref.: #66794]	glutamate and glutamine metabolism	67.86	19 of 28
[Ref.: #66794]	oxidative phosphorylation	67.03	61 of 91
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	methane metabolism	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	tetrahydrofolate metabolism	64.29	9 of 14
[Ref.: #66794]	glutathione metabolism	64.29	9 of 14
[Ref.: #66794]	pentose phosphate pathway	63.64	7 of 11
[Ref.: #66794]	d-xylose degradation	63.64	7 of 11
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	leucine metabolism	61.54	8 of 13
[Ref.: #66794]	degradation of hexoses	61.11	11 of 18
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	propionate fermentation	60	6 of 10
[Ref.: #66794]	methionine metabolism	57.69	15 of 26
[Ref.: #66794]	isoprenoid biosynthesis	57.69	15 of 26
[Ref.: #66794]	citric acid cycle	57.14	8 of 14
[Ref.: #66794]	tryptophan metabolism	55.26	21 of 38
[Ref.: #66794]	alanine metabolism	55.17	16 of 29
[Ref.: #66794]	histidine metabolism	51.72	15 of 29
[Ref.: #66794]	lactate fermentation	50	2 of 4
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	kanosamine biosynthesis II	50	1 of 2
[Ref.: #66794]	adipate degradation	50	1 of 2
[Ref.: #66794]	CMP-KDO biosynthesis	50	2 of 4
[Ref.: #66794]	cysteine metabolism	50	9 of 18
[Ref.: #66794]	sulfopterin metabolism	50	2 of 4
[Ref.: #66794]	arginine metabolism	50	12 of 24
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	non-pathway related	47.37	18 of 38
[Ref.: #66794]	proline metabolism	45.45	5 of 11
[Ref.: #66794]	vitamin B6 metabolism	45.45	5 of 11
[Ref.: #66794]	lipid A biosynthesis	44.44	4 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	44.44	4 of 9
[Ref.: #66794]	tyrosine metabolism	42.86	6 of 14
[Ref.: #66794]	lysine metabolism	42.86	18 of 42
[Ref.: #66794]	factor 420 biosynthesis	40	2 of 5
[Ref.: #66794]	urea cycle	38.46	5 of 13
[Ref.: #66794]	dTDPLrhamnose biosynthesis	37.5	3 of 8
[Ref.: #66794]	lipid metabolism	35.48	11 of 31
[Ref.: #66794]	polyamine pathway	34.78	8 of 23
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	sulfoquinovose degradation	33.33	1 of 3
[Ref.: #66794]	4-hydroxyphenylacetate degradation	30	3 of 10
[Ref.: #66794]	coenzyme M biosynthesis	30	3 of 10
[Ref.: #66794]	metabolism of disaccharids	27.27	3 of 11
[Ref.: #66794]	ascorbate metabolism	27.27	6 of 22
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	sulfate reduction	23.08	3 of 13
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6004]	Sample type/isolated from	oil sample
[Ref.: #6004]	Geographic location (country and/or sea, region)	Queensland, Bowen-Surat Basin, Riverslea oilfield
[Ref.: #6004]	Country	Australia
[Ref.: #6004]	Country ISO 3 Code	AUS
[Ref.: #6004]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Engineered

#Industrial

#Oil reservoir

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY331143 (>99% sequence identity) for Mahella australiensis subclade from Microbeatlas

Aquatic Samples	452
Soil Samples	372
Animal Samples	1911
Plant Samples	96
Total Samples	2831

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6004]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6004]	Mahella australiensis 16S ribosomal RNA gene, partial sequence	AY331143	1531	ENA	697281 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mahella australiensis 50-1 BON, DSM 15567	2503508009	complete	JGI IMG/M	697281 tax ID	*

[Ref.: #6004]

GC-content

55.5 mol%

Availability in culture collections External links

[Ref.: #6004]

Culture collection no.

DSM 15567, CIP 107919

[Ref.: #86980]

SI-ID 134438

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Characterization and efficient production of a thermostable, halostable and organic solvent-stable cellulase from an oil reservoir.	Zhu T, Li R, Sun J, Cui Y, Wu B	Int J Biol Macromol	10.1016/j.ijbiomac.2020.05.021	2020	*
Phylogeny	Distribution and evolution of the mobile vma-1b intein.	Swithers KS, Soucy SM, Lasek-Nesselquist E, Lapierre P, Gogarten JP	Mol Biol Evol	10.1093/molbev/mst164	2013	*
Genetics	Complete genome sequence of Mahella australiensis type strain (50-1 BON).	Sikorski J, Teshima H, Nolan M, Lucas S, Hammon N, Deshpande S, Cheng JF, Pitluck S, Liolios K, Pagani I, Ivanova N, Huntemann M, Mavromatis K, Ovchinikova G, Pati A, Tapia R, Han C, Goodwin L, Chen A, Palaniappan K, Land M, Hauser L, Ngatchou-Djao OD, Rohde M, Pukall R, Spring S, Abt B, Goker M, Detter JC, Woyke T, Bristow J, Markowitz V, Hugenholtz P, Eisen JA, Kyrpides NC, Klenk HP, Lapidus A	Stand Genomic Sci	10.4056/sigs.1864526	2011	*
Phylogeny	Mahella australiensis gen. nov., sp. nov., a moderately thermophilic anaerobic bacterium isolated from an Australian oil well.	Bonilla Salinas M, Fardeau ML, Thomas P, Cayol JL, Patel BKC, Ollivier B	Int J Syst Evol Microbiol	10.1099/ijs.0.02926-0	2004	*

References

#6004

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15567

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30092

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26449 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66793

Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#86980

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID134438.1 )

#26449

IJSEM 2169 2004 ( DOI 10.1099/ijs.0.02926-0 , PubMed 15545453 )

* These data were automatically processed and therefore are not curated

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