Name: Actinomadura hibisca SA 26015
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 16979

Type strain:

Species: Actinomadura hibisca

Strain Designation: SA 26015

Culture col. no.: DSM 44148, ATCC 53557, IFO 15177, NBRC 15177, NCIMB 13253, JCM 9627, HUT 6599, IMSNU 22185

Strain history: NCIMB 13253 <-- ATCC 53557 <-- Bristol-Myers Co.; P157-2 (SA26015).

NCBI tax ID(s): 1220559 (strain), 68565 (species)

SI-ID 125863

ATCC 53557, DSM 44148, IFO 15177, NCIMB 13253, JCM 9627, HUT 6599, NBRC 15177

Other strains from Actinomadura hibisca

A. hibisca ST033258(HKI),

Section

Actinomadura hibisca SA 26015 is a spore-forming, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from soil.

Gram-positive
spore-forming
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Thermomonosporaceae*
	Genus	*Actinomadura*
	Species	**Actinomadura hibisca**
	Full Scientific Name (LPSN)	Actinomadura hibisca Tomita et al. 1991

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #20006]	Colony color	Raspberry red (3027)
[Ref.: #20006]	Incubation period	10-14 days
[Ref.: #20006]	Cultivation medium used	ISP 2

[Ref.: #20006]	Colony color	Raspberry red (3027)
[Ref.: #20006]	Incubation period	10-14 days
[Ref.: #20006]	Cultivation medium used	ISP 3

[Ref.: #20006]	Colony color	Raspberry red (3027)
[Ref.: #20006]	Incubation period	10-14 days
[Ref.: #20006]	Cultivation medium used	ISP 4

[Ref.: #20006]	Colony color	Raspberry red (3027)
[Ref.: #20006]	Incubation period	10-14 days
[Ref.: #20006]	Cultivation medium used	ISP 5

[Ref.: #20006]	Colony color	Raspberry red (3027)
[Ref.: #20006]	Incubation period	10-14 days
[Ref.: #20006]	Cultivation medium used	ISP 6

[Ref.: #20006]	Colony color	Raspberry red (3027)
[Ref.: #20006]	Incubation period	10-14 days
[Ref.: #20006]	Cultivation medium used	ISP 7

[Ref.: #20006]	Multicellular complex forming ability	yes
[Ref.: #20006]	Multicellular complex name	Aerial Mycelium
[Ref.: #20006]	Multicellular complex color	White
[Ref.: #20006]	Medium Name (multicellularity)	ISP 3

[Ref.: #20006]	Multicellular complex forming ability	no
[Ref.: #20006]	Medium Name (multicellularity)	ISP 4

[Ref.: #20006]	Multicellular complex forming ability	no
[Ref.: #20006]	Medium Name (multicellularity)	ISP 5

[Ref.: #20006]	Multicellular complex forming ability	no
[Ref.: #20006]	Medium Name (multicellularity)	ISP 6

[Ref.: #20006]	Multicellular complex forming ability	no
[Ref.: #20006]	Medium Name (multicellularity)	ISP 7

[Ref.: #20006]	Multicellular complex name	Aerial Mycelium
[Ref.: #20006]	Multicellular complex color	White
[Ref.: #20006]	Medium Name (multicellularity)	ISP 2

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11525]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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Culture medium

ISP 2

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 3

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 4

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 5

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 6

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 7

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Culture medium growth

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Culture medium composition

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Culture medium

PEPTON-CORN MEAL MEDIUM (DSMZ Medium 831)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #11525]

Culture medium composition

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[Ref.: #11525]

Culture medium

CZAPEK PEPTONE AGAR (DSMZ Medium 83)

[Ref.: #11525]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #11525]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #11525]	growth	28 °C
[Ref.: #20006]	optimum	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #11525]	Temperature range	mesophilic
[Ref.: #20006]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #20006]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #11525]	Name of produced compound	pradimicin A
[Ref.: #11525]	Name of produced compound	pradimicin B
[Ref.: #11525]	Name of produced compound	pradimicin M
[Ref.: #11525]	Name of produced compound	pradimicin O
[Ref.: #11525]	Name of produced compound	pradimicin N
[Ref.: #11525]	Name of produced compound	pradimicin P
[Ref.: #20216]	Name of produced compound	Pradimicin A
[Ref.: #20216]	Name of produced compound	Pradimicin B
[Ref.: #20216]	Name of produced compound	Pradimicin C
[Ref.: #20006]	Name of produced compound	Pradimicins

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #20006]		NaCl		maximum	2.5	%

[Ref.: #67770]

Observation

quinones: MK-9(H₆)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #20006]	arabinose ChEBI	+
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #20006]	cellulose ChEBI	+
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #20006]	fructose ChEBI	+
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E
[Ref.: #20006]	glucose ChEBI	+
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #20006]	mannitol ChEBI	+
[Ref.: #20006]	myo-inositol ChEBI	+
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #20006]	raffinose ChEBI	+
[Ref.: #20006]	rhamnose ChEBI	+
[Ref.: #20006]	sucrose ChEBI	+
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
[Ref.: #20006]	xylose ChEBI	+
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #67770]	pradimicin ChEBI	yes
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	+	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68368]	gelatinase	+		* from API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #20006]	601	-	+	+	+	+	-	-	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #20006]	795	not determinedn.d.	+	+	+	-	+	+	+	+	+	+	+	-	+	-	+	+	+	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11525]	Sample type/isolated from	soil

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Country	Fiji
[Ref.: #67770]	Country ISO 3 Code	FJI
[Ref.: #67770]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ420136 (>99% sequence identity) for Actinomadura hibisca subclade from Microbeatlas

Aquatic Samples	3
Soil Samples	42
Animal Samples	3
Plant Samples	33
Total Samples	81

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11525]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #20006]	Biosafety level	1	German classification

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Actinomadura hibisca 16S rRNA gene, strain DSM 44148T	AJ420136	1453	ENA	68565 tax ID	*
[Ref.: #20218]	Actinomadura hibisca partial 16S rRNA gene, strain IMSNU 22185T	AJ293705	1516	ENA	68565 tax ID	*
[Ref.: #20218]	Actinomadura hibisca 16S ribosomal RNA gene, partial sequence	AF163115	1457	ENA	68565 tax ID	*
[Ref.: #20218]	Actinomadura hibisca 16S-23S ribosomal RNA intergenic spacer sequence	AF163126	473	ENA	68565 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Actinomadura hibisca NBRC 15177	GCA_001552635	contig	GenBank	1220559 tax ID
[Ref.: #66792]	Actinomadura hibisca NBRC 15177	1220559.3	wgs	PATRIC	1220559 tax ID	*
[Ref.: #66792]	Actinomadura hibisca NBRC 15177	2731957594	draft	JGI IMG/M	1220559 tax ID	*

[Ref.: #67770]

GC-content

72.4 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinomadura hibisca NBRC 15177 PATRIC: 1220559.3
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinomadura hibisca NBRC 15177 PATRIC: 1220559.3
[Ref.: #69480]	motile	no	51.166	no
[Ref.: #69480]	gram-positive	yes	88.056	no
[Ref.: #69480]	anaerobic	no	98.743	no
[Ref.: #69480]	halophile	no	93.778	no
[Ref.: #69480]	spore-forming	yes	94.103	yes
[Ref.: #69480]	glucose-util	yes	88.768	yes
[Ref.: #69480]	flagellated	no	92.449	no
[Ref.: #69480]	aerobic	yes	90.121	no
[Ref.: #69480]	thermophile	no	97.67	no
[Ref.: #69480]	glucose-ferment	no	90.126	no

Availability in culture collections External links

[Ref.: #11525]

Culture collection no.

DSM 44148, ATCC 53557, IFO 15177, NBRC 15177, NCIMB 13253, JCM 9627, HUT 6599, IMSNU 22185

[Ref.: #85983]

SI-ID 125863

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinomadura rubteroloni sp. nov. and Actinomadura macrotermitis sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis.	Benndorf R, Martin K, Kufner M, de Beer ZW, Vollmers J, Kaster AK, Beemelmanns C	Int J Syst Evol Microbiol	10.1099/ijsem.0.004403	2020	*
Enzymology	New antifungal antibiotics, pradimicins D and E. Glycine analogs of pradimicins A and C.	Sawada Y, Nishio M, Yamamoto H, Hatori M, Miyaki T, Konishi M, Oki T	J Antibiot (Tokyo)	10.7164/antibiotics.43.771	1990	*
Pathogenicity	Pradimicins A, B and C: new antifungal antibiotics. II. In vitro and in vivo biological activities.	Oki T, Tenmyo O, Hirano M, Tomatsu K, Kamei H	J Antibiot (Tokyo)	10.7164/antibiotics.43.763	1990	*
Phylogeny	Actinomadura parmotrematis sp. nov., isolated from the foliose lichen, Parmotrema praesorediosum (Nyl.) Hale.	Somphong A, Poengsungnoen V, Buaruang K, Suriyachadkun C, Sripreechasak P, Tanasupawat S, Phongsopitanun W	Int J Syst Evol Microbiol	10.1099/ijsem.0.005447	2022	*

References

#11525

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44148

#20006

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20216

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#85983

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID125863.1 )

* These data were automatically processed and therefore are not curated

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Actinomadura hibisca NBRC 15177

Actinomadura hibisca NBRC 15177

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