Actinomadura hibisca (DSM 44148, ATCC 53557, IFO 15177)

Name: Actinomadura hibisca (DSM 44148, ATCC 53557, IFO 15177)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16979

Type strain

Strain Designation SA 26015, P157-2

Culture col. no. DSM 44148 ATCC 53557 IFO 15177 NBRC 15177 NCIMB 13253 5 more

NCBI tax ID(s) 1220559 68565

Special Collections DSMZ JCM Wink compendium

History <- ATCC <- Bristol-Myers Co., P157-2 NCIMB 13253 <-- ATCC 53557 <-- Bristol-Myers Co.; P157-2 (SA26015).

Links

Actinomadura hibisca SA 26015 is a spore-forming, mesophilic prokaryote that builds an aerial mycelium and was isolated from soil.

spore-forming mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 125863

ATCC 53557,DSM 44148,IFO 15177,NCIMB 13253,JCM 9627,HUT 6599,NBRC 15177

@ref 20215

Last LPSN update 2026-02-09 11:22:06

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Thermomonosporaceae

Genus Actinomadura

Species Actinomadura hibisca

Full scientific name Actinomadura hibisca Tomita et al. 1991

BacDive ID	Other strains from **Actinomadura hibisca** (1)	Type strain
128618	A. hibisca ST033258(HKI),

Morphology

Cell morphology

@ref	Gram stain	Confidence	Motility
125439	positive	99.7
125439		90

Colony morphology

@ref	Colony color	Incubation period	Medium used
20006	Raspberry red (3027)	10-14 days	ISP 2
20006	Raspberry red (3027)	10-14 days	ISP 3
20006	Raspberry red (3027)	10-14 days	ISP 4
20006	Raspberry red (3027)	10-14 days	ISP 5
20006	Raspberry red (3027)	10-14 days	ISP 6
20006	Raspberry red (3027)	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
20006	Aerial Mycelium	White	ISP 2
20006	Aerial Mycelium	White	ISP 3
20006			ISP 4
20006			ISP 5
20006			ISP 6
20006			ISP 7

Multimedia

@ref:	11525
multimedia content:	DSM_44148.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44148.jpg
caption:	Medium 65 28°C
intellectual property rights:	Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11525	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20006	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20006	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20006	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20006	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20006	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20006	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11525	positive	growth	28	mesophilic
20006	positive	optimum	30	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	95

Spore formation

20006

Spore formationyes

Compound production

@ref	Compound
11525	pradimicin A
11525	pradimicin B
11525	pradimicin M
11525	pradimicin O
11525	pradimicin N
11525	pradimicin P
20216	Pradimicin A
20216	Pradimicin B
20216	Pradimicin C
20006	Pradimicins

Halophily

@ref	Salt	Growth	Tested relation	Concentration
20006	NaCl	positive	maximum	2.5 %

Observation

67770

Observationquinones: MK-9(H₆)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
20006	22599 ChEBI	arabinose	+
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
20006	62968 ChEBI	cellulose	+
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
20006	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
20006	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	+	degradation	from API 20E
20006	29864 ChEBI	mannitol	+
20006	17268 ChEBI	myo-inositol	+
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
20006	16634 ChEBI	raffinose	+
20006	26546 ChEBI	rhamnose	+
20006	17992 ChEBI	sucrose	+
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
20006	18222 ChEBI	xylose	+

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E
67770	83230 ChEBI	pradimicin

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	-		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	+	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68382	beta-galactosidase	+	3.2.1.23	from API zym
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
20006	-	+	+	+	+	-	-	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20006	not determinedn.d.	+	+	+	-	+	+	+	+	+	+	+	-	+	-	+	+	+	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
11525	soil
67770	Soil	Fiji	FJI	Australia and Oceania

Taxonmaps

69479

Global distribution of 16S sequence AJ420136 (>99% sequence identity) for Actinomadura hibisca subclade from Microbeatlas

Aquatic Samples	3
Soil Samples	42
Animal Samples	3
Plant Samples	33
Total Samples	81

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11525	1	Risk group (German classification) According to TRBA 466
20006	1	German classification According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM155263v1 assembly for Actinomadura hibisca NBRC 15177	contig	GCA_001552635	1220559.3	2731957594	1220559	39.8

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Actinomadura hibisca 16S rRNA gene, strain DSM 44148T	AJ420136	1453	68565
20218	Actinomadura hibisca partial 16S rRNA gene, strain IMSNU 22185T	AJ293705	1516	68565
20218	Actinomadura hibisca 16S ribosomal RNA gene, partial sequence	AF163115	1457	68565
20218	Actinomadura hibisca 16S-23S ribosomal RNA intergenic spacer sequence	AF163126	473	68565

GC content

@ref	GC-content (mol%)	Method
67770	72.4	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinomadura hibisca NBRC 15177
NCBI: GCA_001552635

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	86.40	no
125439	motility	BacteriaNetⓘ	no	90.00	no
125439	gram_stain	BacteriaNetⓘ	positive	99.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	95.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinomadura hibisca NBRC 15177
PATRIC: 1220559.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	86.30	no
125438	anaerobic	anaerobicⓘ	no	96.17	no
125438	spore-forming	spore-formingⓘ	yes	88.84	yes
125438	aerobic	aerobicⓘ	yes	87.26	no
125438	thermophilic	thermophileⓘ	no	93.75	no
125438	flagellated	motile2+ⓘ	no	60.33	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Synergistic actions of tailoring enzymes in pradimicin biosynthesis.	Napan K, Zhang S, Morgan W, Anderson T, Takemoto JY, Zhan J.	Chembiochem	10.1002/cbic.201402306	2014
	Distribution of thiols in microorganisms: mycothiol is a major thiol in most actinomycetes.	Newton GL, Arnold K, Price MS, Sherrill C, Delcardayre SB, Aharonowitz Y, Cohen G, Davies J, Fahey RC, Davis C.	J Bacteriol	10.1128/jb.178.7.1990-1995.1996	1996
Pathogenicity	In vitro and in vivo antifungal activities of BMY-28864, a water-soluble pradimicin derivative.	Kakushima M, Masuyoshi S, Hirano M, Shinoda M, Ohta A, Kamei H, Oki T.	Antimicrob Agents Chemother	10.1128/aac.35.11.2185	1991
Metabolism	Biosynthesis and functions of mycothiol, the unique protective thiol of Actinobacteria.	Newton GL, Buchmeier N, Fahey RC.	Microbiol Mol Biol Rev	10.1128/mmbr.00008-08	2008
	Draft genome of Thermomonospora sp. CIT 1 (Thermomonosporaceae) and in silico evidence of its functional role in filter cake biomass deconstruction.	Omori WP, Pinheiro DG, Kishi LT, Fernandes CC, Fernandes GC, Gomes-Pepe ES, Pavani CD, Lemos EGM, Souza JAM.	Genet Mol Biol	10.1590/1678-4685-gmb-2017-0376	2019
	The potential biofortification role of Actinopolyspora sp. JTT-01 in enhancing the yield and tissue chemical composition of caraway plants.	Mahmoud AM, Sheteiwy MS, El-Keblawy A, Ulhassan Z, Khalaf MH, Mohamed HS, Okla MK, AlGarawi AM, El-Sawah AM, Ahmed ES, Reyad AM.	BMC Plant Biol	10.1186/s12870-025-06137-1	2025
	Investigating the Endophyte Actinomycetota sp. JW0824 Strain as a Potential Bioinoculant to Enhance the Yield, Nutritive Value, and Chemical Composition of Different Cultivars of Anise (Pimpinella anisum L.) Seeds.	Mahmoud AM, Reyad AM, Khalaf MH, Sheteiwy MS, Dawood MFA, El-Sawah AM, Shaban Ahmed E, Malik A, Al-Qahtani WH, Abdel-Maksoud MA, Mousa NHS, Alyafei M, AbdElgawad H.	Biology (Basel)	10.3390/biology13080553	2024
Enzymology	New antifungal antibiotics, pradimicins D and E. Glycine analogs of pradimicins A and C.	Sawada Y, Nishio M, Yamamoto H, Hatori M, Miyaki T, Konishi M, Oki T	J Antibiot (Tokyo)	10.7164/antibiotics.43.771	1990
Pathogenicity	Pradimicins A, B and C: new antifungal antibiotics. II. In vitro and in vivo biological activities.	Oki T, Tenmyo O, Hirano M, Tomatsu K, Kamei H	J Antibiot (Tokyo)	10.7164/antibiotics.43.763	1990
Genetics	Pradimicin U, a promising antimicrobial agent isolated from a newly found Nonomuraea composti sp. nov.	Duangupama T, Pittayakhajonwut P, Intaraudom C, Suriyachadkun C, Tadtong S, Kuncharoen N, He YW, Tanasupawat S, Thawai C.	Sci Rep	10.1038/s41598-024-60744-w	2024
Phylogeny	Actinomadura rubteroloni sp. nov. and Actinomadura macrotermitis sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis.	Benndorf R, Martin K, Kufner M, de Beer ZW, Vollmers J, Kaster AK, Beemelmanns C	Int J Syst Evol Microbiol	10.1099/ijsem.0.004403	2020
Phylogeny	Actinomadura parmotrematis sp. nov., isolated from the foliose lichen, Parmotrema praesorediosum (Nyl.) Hale.	Somphong A, Poengsungnoen V, Buaruang K, Suriyachadkun C, Sripreechasak P, Tanasupawat S, Phongsopitanun W	Int J Syst Evol Microbiol	10.1099/ijsem.0.005447	2022

References

#11525	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44148
#20006	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) . Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinomadura hibisca (DSM 44148, ATCC 53557, IFO 15177)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001552635

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

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