Actinomadura verrucosospora (DSM 43358, ATCC 27299, DSM 43550)

Name: Actinomadura verrucosospora (DSM 43358, ATCC 27299, DSM 43550)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16963

Type strain

Strain Designation A-184, A4, A-0147

Culture col. no. DSM 43358 ATCC 27299 DSM 43550 KCC A-0147 A-184 22 more

NCBI tax ID(s) 46165

Special Collections DSMZ JCM CIP Wink compendium JMRC

History DSMZ <- M. Goodfellow, A4 <- H. Nonomura, FYU, A-184 JCM <-- KCC A-0147 <-- H. Nonomura A-184. CIP <- 1998, JCM <- KCC: strain A-0147 <- H. Nonomura: strain A-184

Links

Actinomadura verrucosospora A-184 is an obligate aerobe, spore-forming, Gram-positive bacterium that builds an aerial mycelium and was isolated from soil.

spore-forming Gram-positive rod-shaped obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 76139

MTCC 1397,ATCC 27299,NRRL B-16129,CIP 105480,CCRC 13408,DSM 43358,DSM 43550,IFO 14100,IMET 9588,JCM 3147,NCIMB 11119,VKM Ac-668,CCM 3492,CBS 258.72,NBRC 14100,LMD 258.72,BCRC 13408,CGMCC 4.2116,CGMCC 4.1512

@ref 20215

Last LPSN update 2026-05-29 08:28:54

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Thermomonosporaceae

Genus Actinomadura

Species Actinomadura verrucosospora

Full scientific name Actinomadura verrucosospora Nonomura and Ohara 1971 (Approved Lists 1980)

BacDive ID	Other strains from **Actinomadura verrucosospora** (3)	Type strain
16964	A. verrucosospora 831-39, A 194, DSM 46190, IMET 9620
128609	A. verrucosospora STI09619(IMET), A193, D2, 831-038, IMET 9619
128611	A. verrucosospora STH00309, HKI 0309

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
125439	positive			97.098
123823	positive	rod-shaped

Colony morphology

@ref	Colony color	Incubation period	Medium used
19569		10-14 days	ISP 2
19569		10-14 days	ISP 3
19569		10-14 days	ISP 4
19569	Antique pink	10-14 days	ISP 5
19569	Antique pink	10-14 days	ISP 6
19569	Antique pink	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19569	Aerial Mycelium	White	ISP 2
19569	Aerial Mycelium	White	ISP 3
19569	Aerial Mycelium	White	ISP 4
19569			ISP 5
19569			ISP 6
19569			ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
19569	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19569	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19569	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19569	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19569	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19569	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
40185	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
10978	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
10978	OAT FLAKES AGAR WITH YEAST EXTRACT (DSMZ Medium 248)	Medium recipe at MediaDive	Name: OAT FLAKES AGAR WITH YEAST EXTRACT (DSMZ Medium 248) Composition: Agar 20.0 g/l Oat flakes 20.0 g/l Yeast extract 2.0 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water
123823	CIP Medium 3	Medium recipe at CIP
123823	CIP Medium 9	Medium recipe at CIP
123823	CIP Medium 275	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
10978	positive	growth	37
19569	positive	optimum	28
40185	positive	growth	37
67770	positive	growth	35
123823	positive	growth	15-37
123823	negative	growth	5
123823	negative	growth	41

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
123823	obligate aerobe
125439	obligate aerobe	98.744

Spore formation

@ref	Spore description	Type of spore	Spore formation
19569	Formation of spore chains (spirales)(Sp), spore surface warty	spore

Compound production

@ref	Compound
19569	SF 2487
19569	Esperamicin
19569	Pradimicin L
19569	Verucopeptin

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19569	NaCl	positive	maximum	2.5 %
123823	NaCl	positive	growth	0-2 %
123823	NaCl		growth	4 %
123823	NaCl		growth	6 %
123823	NaCl		growth	8 %
123823	NaCl		growth	10 %

Observation

67770

Observationquinones: MK-9(H₆), MK-9(H₈), MK-9(H₆)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
19569	22599 ChEBI	arabinose	+
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
19569	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
123823	4853 ChEBI	esculin	+	hydrolysis
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
19569	28757 ChEBI	fructose	+/-
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
19569	17234 ChEBI	glucose	+
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
19569	29864 ChEBI	mannitol	+
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
19569	17268 ChEBI	myo-inositol	+
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
123823	17632 ChEBI	nitrate	-	reduction
123823	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
19569	16634 ChEBI	raffinose	-
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
19569	26546 ChEBI	rhamnose	+
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
19569	17992 ChEBI	sucrose	+/-
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid
19569	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
123823	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test	Indole test
123823	15688 ChEBI	acetoin	-
68368	15688 ChEBI	acetoin	+			from API 20E
123823	17234 ChEBI	glucose		-
68368	35581 ChEBI	indole			-	from API 20E

Enzymes

@ref	Value	Activity	Ec
123823	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123823	amylase	-
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68382	beta-galactosidase	-	3.2.1.23	from API zym
123823	beta-galactosidase	-	3.2.1.23
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123823	caseinase	+	3.4.21.50
123823	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
123823	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
123823	gamma-glutamyltransferase	-	2.3.2.2
123823	gelatinase	+/-
68368	gelatinase	+		from API 20E
123823	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
123823	lipase	-
68382	lipase (C 14)	+		from API zym
123823	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123823	ornithine decarboxylase	-	4.1.1.17
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
123823	oxidase	-
123823	phenylalanine ammonia-lyase	-	4.3.1.24
123823	protease	-
123823	tryptophan deaminase	-
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
123823	tween esterase	-
123823	urease	-	3.5.1.5
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19569	-	+	-	-	+	-	-	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19569	not determinedn.d.	+	+	+	+	+	+	+	+	+	+	+	-	-	-	+	+	+	-	-
123823	-	+	+	+	+	+	+	+	-	+	-	+	-	-	-	+	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
123823	not determinedn.d.	-	-	-	-	-	+/-	-	-	-	-	+/-	-	-	-	+/-	-	-	-	-	-	-	+/-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
123823	not determinedn.d.	+	-	-	-	-	-	-	-	-	+	+	-	-	-	-	+	-	-	+	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
10978	soil
67770	Soil, dry-heated	Yamanashi Pref.	Japan	JPN	Asia
123823	Environment, Soil	Yamanashi	Japan	JPN	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
10978	1	Risk group (German classification) According to TRBA 466
19569	1	Risk group (German classification) According to TRBA 466
123823	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM4243040v1 assembly for Actinomadura verrucosospora CCM 3492	scaffold	GCA_042430405	46165	62.57
124043	ASM3954033v1 assembly for Actinomadura verrucosospora JCM 3147	scaffold	GCA_039540335	46165	41.71

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Actinomadura verrucosospora 16S-23S ribosomal RNA intergenic spacer region, partial sequence	AF134106	448	46165
20218	Actinomadura verrucospora 16S rRNA gene	U49011	1411	46165
20218	Actinomadura verrucosospora DNA for 16S rRNA, partial sequence	D50667	1339	46165
124043	Actinomadura verrucosospora strain CCM 3492 16S ribosomal RNA gene, partial sequence.	MT758000	1368	46165
124043	Actinomadura verrucosospora strain CCM 3492 16S ribosomal RNA gene, partial sequence.	MT760073	1368	46165

GC content

@ref	GC-content (mol%)	Method
67770	69	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM4243040v1 assembly for Actinomadura verrucosospora CCM 3492
NCBI: GCA_042430405

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.74	no
125439	gram_stain	BacteriaNetⓘ	positive	97.10	no
125439	motility	BacteriaNetⓘ	no	86.66	no
125439	spore_formation	BacteriaNetⓘ	yes	54.03	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	The biosynthetic pathway to tetromadurin (SF2487/A80577), a polyether tetronate antibiotic.	Little RF, Samborskyy M, Leadlay PF.	PLoS One	10.1371/journal.pone.0239054	2020
	Thioesterase enzyme families: Functions, structures, and mechanisms.	Caswell BT, de Carvalho CC, Nguyen H, Roy M, Nguyen T, Cantu DC.	Protein Sci	10.1002/pro.4263	2022
Genetics	Genome neighborhood network reveals insights into enediyne biosynthesis and facilitates prediction and prioritization for discovery.	Rudolf JD, Yan X, Shen B.	J Ind Microbiol Biotechnol	10.1007/s10295-015-1671-0	2016
Enzymology	Pradimicins L and FL: new pradimicin congeners from Actinomadura verrucosospora subsp. neohibisca.	Saitoh K, Sawada Y, Tomita K, Tsuno T, Hatori M, Oki T.	J Antibiot (Tokyo)	10.7164/antibiotics.46.387	1993
Metabolism	The effect of cerulenin on the production of esperamicin A1 by Actinomadura verrucosospora.	Lam KS, Gustavson DR, Veitch JA, Forenza S.	J Ind Microbiol	10.1007/bf01569908	1993
Metabolism	Esperamicin P, the tetrasulfide analog of esperamicin A1.	Beutler JA, Clark P, Alvarado AB, Golik J.	J Nat Prod	10.1021/np50107a010	1994
Enzymology	Microbial modification of pradimicins at C-11 leading to 11-O-demethyl- and 11-O-L-xylosylpradimicins A and FA-1.	Furumai T, Yamamoto H, Narita Y, Hasegawa T, Aburaki S, Kakushima M, Oki T.	J Antibiot (Tokyo)	10.7164/antibiotics.46.1589	1993
Metabolism	Biosynthesis of the pradimicin family of antibiotics. I. Generation and selection of pradimicin-nonproducing mutants.	Furumai T, Kakinuma S, Yamamoto H, Komiyama N, Suzuki K, Saitoh K, Oki T.	J Antibiot (Tokyo)	10.7164/antibiotics.46.412	1993
Metabolism	Biosynthesis of the pradimicin family of antibiotics. II. Fermentation, isolation and structure determination of metabolites associated with the pradimicins biosynthesis.	Tsuno T, Yamamoto H, Narita Y, Suzuki K, Hasegawa T, Kakinuma S, Saitoh K, Furumai T, Oki T.	J Antibiot (Tokyo)	10.7164/antibiotics.46.420	1993
Enzymology	Development of a self-cloning system for Actinomadura verrucosospora and identification of polyketide synthase genes essential for production of the angucyclic antibiotic pradimicin.	Dairi T, Hamano Y, Furumai T, Oki T.	Appl Environ Microbiol	10.1128/aem.65.6.2703-2709.1999	1999
	Experimental antitumor activity of BMY-28175 a new fermentation derived antitumor agent.	Schurig JE, Rose WC, Kamei H, Nishiyama Y, Bradner WT, Stringfellow DA.	Invest New Drugs	10.1007/bf00216919	1990
	Potent antitumor antibiotic complex: PD 114,759, PD 115,028, PD 119,707, and PD 119,193.	Tunac JB, Graham BD, Mamber SW, Dobson WE, Lenzini MD.	J Antibiot (Tokyo)	10.7164/antibiotics.38.1337	1985
	[Electron microscopic study of spore formation in Actinomadura verrucosospora].	Soina VS, Sokolov AA, Agre NS.	Mikrobiologiia		1977
Phylogeny	Molecular-genetic and chemotaxonomic studies on Actinomadura and Nocardiopsis.	Fischer A, Kroppenstedt RM, Stackebrandt E.	J Gen Microbiol	10.1099/00221287-129-11-3433	1983
	[New elementary structures of actinomycete spores of the genera Actinomadura and Streptomyces].	Soina VS, Agre NS.	Mikrobiologiia		1979
	Polyketide synthase chemistry does not direct biosynthetic divergence between 9- and 10-membered enediynes.	Horsman GP, Chen Y, Thorson JS, Shen B.	Proc Natl Acad Sci U S A	10.1073/pnas.1003442107	2010
Phylogeny	Rapid PCR amplification of minimal enediyne polyketide synthase cassettes leads to a predictive familial classification model.	Liu W, Ahlert J, Gao Q, Wendt-Pienkowski E, Shen B, Thorson JS.	Proc Natl Acad Sci U S A	10.1073/pnas.2034291100	2003
	Genetic dissection of the biosynthetic route to gentamicin A2 by heterologous expression of its minimal gene set.	Park JW, Hong JS, Parajuli N, Jung WS, Park SR, Lim SK, Sohng JK, Yoon YJ.	Proc Natl Acad Sci U S A	10.1073/pnas.0803164105	2008
	Efficient and accurate characterization of the Bergman cyclization for several enediynes including an expanded substructure of esperamicin A1.	Sherer EC, Kirschner KN, Pickard FC, Rein C, Feldgus S, Shields GC.	J Phys Chem B	10.1021/jp807341t	2008
Enzymology	Distribution of beta-lactamases in actinomycetes.	Ogawara H, Kawamura N, Kudo T, Suzuki KI, Nakase T.	Antimicrob Agents Chemother	10.1128/aac.43.12.3014	1999
Genetics	Pradimicin U, a promising antimicrobial agent isolated from a newly found Nonomuraea composti sp. nov.	Duangupama T, Pittayakhajonwut P, Intaraudom C, Suriyachadkun C, Tadtong S, Kuncharoen N, He YW, Tanasupawat S, Thawai C.	Sci Rep	10.1038/s41598-024-60744-w	2024

References

#10978	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43358
#19569	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#40185	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#123823	Collection of Institut Pasteur ; Curators of the CIP; CIP 105480
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinomadura verrucosospora (DSM 43358, ATCC 27299, DSM 43550)

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