Cite this strain

"Thermococcus radiotolerans" (DSM 15228, JCM 11826, EJ2)

BacDive ID 16884

Type strain

Strain Designation EJ2

Culture col. no. DSM 15228 JCM 11826 EJ2

NCBI tax ID(s) 187880

Special Collections DSMZ JCM

Links

"Thermococcus radiotolerans" EJ2 is an anaerobe, hyperthermophilic prokaryote that was isolated from chimney of deep-sea hydrothermal vent.

anaerobe hyperthermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 231970

JCM 11826,DSM 15228

@ref 20215

Last LPSN update 2025-12-16 09:50:57

Domain Methanobacteriati

Phylum Methanobacteriota

Class Thermococci

Order Thermococcales

Family Thermococcaceae

Genus Thermococcus

Species "Thermococcus radiotolerans"

Full scientific name Thermococcus radiotolerans Jolivet et al. 2004

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	94.7

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
5768	MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760)		Medium recipe at MediaDive	Name: MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
5768	YPS MEDIUM (DSMZ Medium 990)		Medium recipe at MediaDive	Name: YPS MEDIUM (DSMZ Medium 990) Composition: Sea Salt 35.0 g/l Elemental sulphur 5.0 g/l Peptone 4.0 g/l PIPES 3.46 g/l Yeast extract 1.0 g/l NH4Cl 0.5 g/l KH2PO4 0.35 g/l Na2S x 9 H2O 0.25 g/l CaCl2 0.2 g/l FeCl3 0.0067 g/l Na2WO4 0.0029 g/l Resazurin 0.0001 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
5768	positive	growth	88	hyperthermophilic
67770	positive	growth	85	hyperthermophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
5768	anaerobe
125439	anaerobe

Spore formation

@ref	Spore formation	Confidence
125439		95.4
125438		91.136

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Hydrothermal vent
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Latitude	Longitude
5768	chimney of deep-sea hydrothermal vent	Guayma Basin, 27°01`N 111°24`W	27.0167	-111.4 27.0167/-111.4
67770	Chimney-wall from hydrothermal site in the Guaymas Basin

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5768	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM221456v1 assembly for Thermococcus radiotolerans EJ2	complete	GCA_002214565	187880.4	2758568021	187880	99.41

Genome browser: GCA_002214565

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
5768	Thermococcus radiophilus 16S ribosomal RNA gene, partial sequence	AF479013	1422		187880

GC content

@ref	GC-content (mol%)
5768	54.5
67770	54.5

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Thermococcus radiotolerans EJ2
NCBI: GCA_002214565

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	95.40	no
125439	motility	BacteriaNetⓘ	no	67.20	no
125439	gram_stain	BacteriaNetⓘ	negative	94.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Thermococcus radiotolerans EJ2
NCBI: GCA_002214565.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	69.43	no
125438	anaerobic	anaerobicⓘ	yes	87.82	yes
125438	aerobic	aerobicⓘ	no	86.56	no
125438	spore-forming	spore-formingⓘ	no	91.14	no
125438	thermophilic	thermophileⓘ	yes	82.27	yes
125438	flagellated	motile2+ⓘ	no	79.58	no

Literature

Title	Authors	Journal	Year
Biochemical and in silico evaluation of a recombinant, glucose tolerant, and highly thermostable beta-glucosidase from Thermococcus radiotolerans DSM-15228	Albalawi H, Altayeb HN, Iftikhar S, Al-Ghamdi MAA, Khan JA, Nadeem MS.	Electron J Biotechnol	2023
Thermococcus marinus sp. nov. and Thermococcus radiotolerans sp. nov., two hyperthermophilic archaea from deep-sea hydrothermal vents that resist ionizing radiation.	Jolivet E, Corre E, L'Haridon S, Forterre P, Prieur D	Extremophiles	2004

References

#5768	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15228
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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"Thermococcus radiotolerans" (DSM 15228, JCM 11826, EJ2)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002214565

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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