Name: Thermococcus radiotolerans EJ2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 16884

Type strain:

Species: Thermococcus radiotolerans

Strain Designation: EJ2

Culture col. no.: DSM 15228, JCM 11826

Strain history: E. Jolivet EJ2.

NCBI tax ID(s): 187880 (species)

Section

Thermococcus radiotolerans EJ2 is an anaerobe, hyperthermophilic, Gram-negative archaeon that was isolated from chimney of deep-sea hydrothermal vent.

Gram-negative
anaerobe
hyperthermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Thermococci*
	Order	*Thermococcales*
	Family	*Thermococcaceae*
	Genus	*Thermococcus*
	Species	"Thermococcus radiotolerans"
	Full Scientific Name (LPSN)	Thermococcus radiotolerans Jolivet et al. 2004

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.939

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5768]

Culture medium

MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760)

[Ref.: #5768]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #5768]

Culture medium composition

expand / minimize

[Ref.: #5768]

Culture medium

YPS MEDIUM (DSMZ Medium 990)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #5768]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #5768]			growth	88 °C
[Ref.: #67770]			growth	85 °C

[Ref.: #5768]	Temperature range	hyperthermophilic
[Ref.: #67770]	Temperature range	hyperthermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %100

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5768]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.072

[Ref.: #69481]	Ability of spore formation	no Confidence in %94
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.62

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5768]	Sample type/isolated from	chimney of deep-sea hydrothermal vent
[Ref.: #5768]	Geographic location (country and/or sea, region)	Guayma Basin, 27°01`N 111°24`W
[Ref.: #5768]	Geographic location	27.0167°/-111.4000°

[Ref.: #67770]	Sample type/isolated from	Chimney-wall from hydrothermal site in the Guaymas Basin
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Hydrothermal vent
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5768]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5768]	Thermococcus radiophilus 16S ribosomal RNA gene, partial sequence	AF479013	1422	ENA	187880 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Thermococcus radiotolerans EJ2	GCA_002214565	complete	GenBank	187880 tax ID	*
[Ref.: #66792]	Thermococcus radiotolerans EJ2	2758568021	complete	JGI IMG/M	187880 tax ID	*

[Ref.: #5768]	GC-content	54.5 mol%
[Ref.: #67770]	GC-content	54.5 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Thermococcus radiotolerans EJ2 NCBI: GCA_002214565.1
[Ref.: #69481]	spore-forming	no	94	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thermococcus radiotolerans EJ2 NCBI: GCA_002214565.1
[Ref.: #69480]	flagellated	no	96.301	no
[Ref.: #69480]	gram-positive	no	96.056	no
[Ref.: #69480]	anaerobic	yes	99.211	no
[Ref.: #69480]	aerobic	no	98.17	no
[Ref.: #69480]	halophile	no	76.867	no
[Ref.: #69480]	spore-forming	no	96.792	no
[Ref.: #69480]	glucose-util	yes	79.94	no
[Ref.: #69480]	thermophile	yes	100	no
[Ref.: #69480]	motile	no	79.444	no
[Ref.: #69480]	glucose-ferment	no	52.242	no

Availability in culture collections External links

[Ref.: #5768]

Culture collection no.

DSM 15228, JCM 11826

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Thermococcus marinus sp. nov. and Thermococcus radiotolerans sp. nov., two hyperthermophilic archaea from deep-sea hydrothermal vents that resist ionizing radiation.	Jolivet E, Corre E, L'Haridon S, Forterre P, Prieur D	Extremophiles	10.1007/s00792-004-0380-9	2004	*

References

#5768

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15228

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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