Name: Neorhizobium lilium 24NR
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 166100

Type strain:

Species: Neorhizobium lilium

Strain Designation: 24NR

Culture col. no.: ACCC 61588, JCM 33731

NCBI tax ID(s): 2503024 (species)

Special Collections

Section

Neorhizobium lilium 24NR is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from bulbs of Lilium pumilum.

colony-forming
Gram-negative
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Rhizobiaceae*
	Genus	*Neorhizobium*
	Species	"Neorhizobium lilium"
	Full Scientific Name (LPSN)	Neorhizobium lilium Liu et al. 2020

Information on morphological and physiological properties Morphology

[Ref.: #68262]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %97.333

[Ref.: #68262]	Cell length	0.7-1.7 µm

[Ref.: #68262]	Cell width	0.3-0.5 µm

[Ref.: #68262]	Cell shape	rod-shaped

[Ref.: #68262]	Motility	no

[Ref.: #68262]	Colony size	2-3 mm
[Ref.: #68262]	Colony shape	circular
[Ref.: #68262]	Incubation period	2 days
[Ref.: #68262]	Cultivation medium used	YMA

Information on culture and growth conditions Culture and growth conditions

	Temperatures	Kind of temperature		Temperature
[Ref.: #68262]			growth	15-42 °C
[Ref.: #68262]			optimum	30 °C

	pH	Kind of pH		pH
[Ref.: #68262]			optimum	7-8
[Ref.: #68262]			growth	6-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #68262]

Oxygen tolerance

aerobe

[Ref.: #68262]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #68262]		NaCl		growth	0-2	%(w/v)
[Ref.: #68262]		NaCl		optimum	0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68262]	3-O-methyl-D-glucose ChEBI	+/-	carbon source
[Ref.: #68262]	acetic acid ChEBI	+	carbon source
[Ref.: #68262]	acetoacetate ChEBI	+	carbon source
[Ref.: #68262]	arabinose ChEBI	+	assimilation
[Ref.: #68262]	beta-D-glucuronic acid ChEBI	+	carbon source
[Ref.: #68262]	beta-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #68262]	bromosuccinate ChEBI	+	carbon source
[Ref.: #68262]	casein	-	hydrolysis
[Ref.: #68262]	cellobiose ChEBI	+	carbon source
[Ref.: #68262]	D-fructose ChEBI	+	carbon source
[Ref.: #68262]	D-galactose ChEBI	+	carbon source
[Ref.: #68262]	D-galacturonic acid ChEBI	+	carbon source
[Ref.: #68262]	D-mannose ChEBI	+	carbon source
[Ref.: #68262]	gentiobiose ChEBI	+	carbon source
[Ref.: #68262]	glucose ChEBI	+	assimilation
[Ref.: #68262]	glycyl-L-proline ChEBI	+	carbon source
[Ref.: #68262]	L-alanine ChEBI	+	carbon source
[Ref.: #68262]	L-arginine-2-naphthylamide ChEBI	+/-	carbon source
[Ref.: #68262]	L-aspartate ChEBI	+	carbon source
[Ref.: #68262]	L-malate ChEBI	+	carbon source
[Ref.: #68262]	L-serine ChEBI	+	carbon source
[Ref.: #68262]	malate ChEBI	+	assimilation
[Ref.: #68262]	mannitol ChEBI	+	assimilation
[Ref.: #68262]	mannose ChEBI	+	assimilation
[Ref.: #68262]	mannose ChEBI	-	fermentation
[Ref.: #68262]	melibiose ChEBI	-	fermentation
[Ref.: #68262]	myo-inositol ChEBI	+	carbon source
[Ref.: #68262]	nitrate ChEBI	-	reduction
[Ref.: #68262]	rhamnose ChEBI	-	fermentation
[Ref.: #68262]	sorbitol ChEBI	-	fermentation
[Ref.: #68262]	starch ChEBI	-	hydrolysis
[Ref.: #68262]	turanose ChEBI	+/-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68262]		acetoin ChEBI	yes

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68262]		acetoin ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #68262]	acid phosphatase	+	3.1.3.2
[Ref.: #68262]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68262]	beta-galactosidase	-	3.2.1.23
[Ref.: #68262]	beta-glucuronidase	+	3.2.1.31
[Ref.: #68262]	catalase	+	1.11.1.6
[Ref.: #68262]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68262]	leucine arylamidase	+	3.4.11.1
[Ref.: #68262]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #68262]	urease	-	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #68262]

Fatty acid	Percentage
C18:1 w7c/C18:1 w6c	35.3
C19:0 cyclo w8c	30
C16:0	8
C14:0 3OH/iso-C16:1 I	5.6
C16:0 3OH	4.3
C16:1 w7c/C16:1 w6c	2.5
C18:1 11-methyl w7c	2.4
C18:1 2OH	2.2
C20:2 w6,9c	1.7
C18:0 3OH	1.6
C17:0 cyclo	1.2
C18:0	1.1
C19:1 w7c / C19:1 w6c	1.1
C16:1 w11c	1
C12:0	0.9
C19:0	0.5
C11:0 2OH	0.5
C14:0	0.4

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #68262]	Sample type/isolated from	bulbs of Lilium pumilum
[Ref.: #68262]	Geographic location (country and/or sea, region)	Hebei Province
[Ref.: #68262]	Country	China
[Ref.: #68262]	Country ISO 3 Code	CHN
[Ref.: #68262]	Continent	Asia
[Ref.: #68262]	Enrichment culture	R2A and NA
[Ref.: #68262]	Enrichment culture duration	5-7 days
[Ref.: #68262]	Enrichment culture temperature	30 ̊C
* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #68262]	Neorhizobium lilium strain 24NR 16S ribosomal RNA gene, partial sequence	MK386721	1471	GenBank ENA	391 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Neorhizobium lilium 24NR	GCA_004053875	scaffold	GenBank ENA	2503024 tax ID	*

[Ref.: #68262]

GC-content

60.3 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Neorhizobium lilium 24NR NCBI: GCA_004053875.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	97.333	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	95.709	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	82.679	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	87.886	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96.014	no
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	72.359	no

Availability in culture collections External links

[Ref.: #68262]

Culture collection no.

ACCC 61588, JCM 33731

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Neorhizobium lilium sp. nov., an endophytic bacterium isolated from Lilium pumilum bulbs in Hebei province.	Liu L, Shi S, Liang L, Xu L, Chi M, Zhang X, Li L	Arch Microbiol	10.1007/s00203-019-01774-1	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68262

Lei Liu, Shaowen Shi, Lixiong Liang, Lijun Xu, Miao Chi, Xiaoxia Zhang & Lubin Li: Neorhizobium lilium sp. nov., an endophytic bacterium isolated from Lilium pumilum bulbs in Hebei province. Arch Microbiol 202: 609 - 616 2020 ( DOI 10.1007/s00203-019-01774-1 )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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