Name: Oceanobacillus piezotolerans YLB-02
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 164402

Type strain:

Species: Oceanobacillus piezotolerans

Strain Designation: YLB-02

Culture col. no.: JCM 32870, MCCC 1A12699

Strain history: X. Tang; Third Inst. of State Oceanic Admin., China; YLB-02.

NCBI tax ID(s): 2448030 (species)

Section

Oceanobacillus piezotolerans YLB-02 is an aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from Deep-sea sediment at 4435 m of the Yap Trench.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Oceanobacillus*
	Species	**Oceanobacillus piezotolerans**
	Full Scientific Name (LPSN)	Oceanobacillus piezotolerans Yu et al. 2019

Information on morphological and physiological properties Morphology

[Ref.: #68177]	Gram stain	positive

[Ref.: #68177]	Cell length	1.2-3.0 µm

[Ref.: #68177]	Cell width	0.6-0.7 µm

[Ref.: #68177]	Cell shape	rod-shaped

[Ref.: #68177]	Motility	yes

[Ref.: #68177]	Flagellum arrangement	monotrichous

[Ref.: #68177]	Colony size	1-2 mm
[Ref.: #68177]	Colony color	cream
[Ref.: #68177]	Colony shape	circular
[Ref.: #68177]	Incubation period	1 day
[Ref.: #68177]	Cultivation medium used	Marine agar (MA)

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #67770]	growth	37 °C
[Ref.: #68177]	growth	15-50 °C
[Ref.: #68177]	optimum	37 °C

[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #68177]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #68177]			optimum	7.5
[Ref.: #68177]			growth	5-10.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #68177]

Oxygen tolerance

aerobe

[Ref.: #68177]	Type of spore	endospore
[Ref.: #68177]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #68177]		NaCl		growth	0-9	%(w/v)
[Ref.: #68177]		NaCl		optimum	5	%(w/v)

[Ref.: #67770]	Observation	quinones: MK-7
[Ref.: #68177]	Observation	Ellipsoidal spores are central within swollen sporangia
[Ref.: #68177]	Observation	Growth occurs within the hydropressure range 0.1-50 MPa (optimal growth at 0.1 MPa)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68177]	amygdalin ChEBI	-	assimilation
[Ref.: #68177]	arbutin ChEBI	-	assimilation
[Ref.: #68177]	cellobiose ChEBI	-	assimilation
[Ref.: #68177]	cellulose ChEBI	-	hydrolysis
[Ref.: #68177]	D-arabitol ChEBI	-	assimilation
[Ref.: #68177]	D-fructose ChEBI	+	assimilation
[Ref.: #68177]	D-galactose ChEBI	+	assimilation
[Ref.: #68177]	D-mannose ChEBI	-	assimilation
[Ref.: #68177]	D-ribose ChEBI	-	assimilation
[Ref.: #68177]	D-tagatose ChEBI	-	assimilation
[Ref.: #68177]	D-xylose ChEBI	-	assimilation
[Ref.: #68177]	esculin ChEBI	+	hydrolysis
[Ref.: #68177]	esculin ferric citrate	+	assimilation
[Ref.: #68177]	gelatin ChEBI	+	hydrolysis
[Ref.: #68177]	gentiobiose ChEBI	-	assimilation
[Ref.: #68177]	glycerol ChEBI	-	assimilation
[Ref.: #68177]	L-arabitol ChEBI	-	assimilation
[Ref.: #68177]	L-fucose ChEBI	-	assimilation
[Ref.: #68177]	L-rhamnose ChEBI	-	assimilation
[Ref.: #68177]	lactose ChEBI	+	assimilation
[Ref.: #68177]	maltose ChEBI	-	assimilation
[Ref.: #68177]	methyl alpha-D-glucopyranoside ChEBI	+	assimilation
[Ref.: #68177]	methyl alpha-D-mannoside ChEBI	-	assimilation
[Ref.: #68177]	potassium 2-ketogluconate	-	assimilation
[Ref.: #68177]	Potassium 5-ketogluconate	-	assimilation
[Ref.: #68177]	potassium gluconate ChEBI	-	assimilation
[Ref.: #68177]	raffinose ChEBI	-	assimilation
[Ref.: #68177]	salicin ChEBI	-	assimilation
[Ref.: #68177]	skimmed milk	-	hydrolysis
[Ref.: #68177]	starch ChEBI	-	hydrolysis
[Ref.: #68177]	starch ChEBI	-	assimilation
[Ref.: #68177]	sucrose ChEBI	-	assimilation
[Ref.: #68177]	turanose ChEBI	+	assimilation
[Ref.: #68177]	tween 20 ChEBI	+	hydrolysis
[Ref.: #68177]	tween 40 ChEBI	+	hydrolysis
[Ref.: #68177]	tween 60 ChEBI	+	hydrolysis
[Ref.: #68177]	tween 80 ChEBI	+	hydrolysis
[Ref.: #68177]	xylitol ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68177]		acetoin ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68177]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68177]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68177]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #68177]	alpha-galactosidase	-	3.2.1.22
[Ref.: #68177]	alpha-glucosidase	+	3.2.1.20
[Ref.: #68177]	beta-galactosidase	+	3.2.1.23
[Ref.: #68177]	beta-glucosidase	-	3.2.1.21
[Ref.: #68177]	beta-glucuronidase	+	3.2.1.31
[Ref.: #68177]	cystine arylamidase	-	3.4.11.3
[Ref.: #68177]	esterase Lipase (C 8)	-
[Ref.: #68177]	leucine arylamidase	+	3.4.11.1
[Ref.: #68177]	lipase (C 14)	-
[Ref.: #68177]	naphthol-AS-BI-phosphohydrolase	-
[Ref.: #68177]	nitrate reductase	-	1.7.99.4
[Ref.: #68177]	trypsin	+	3.4.21.4
[Ref.: #68177]	urease	+	3.5.1.5
[Ref.: #68177]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #68177]

Fatty acid	Percentage
C14:0 iso	24.5
C16:0 iso	19.4
C15:0 iso	14.2
C16:1 w7c alcohol	11.7
C16:0	4.7
C15:0 anteiso	4.6
C18:0	4.3
C18:1 w7c/C18:1 w6c	2.6
C17:0 iso	1.6
C12:0 iso	1.6
C16:0 2OH	1.6
C11:0	1.6
C16:1 w11c	1.4
C18:1 w9c	1.3
C13:0 iso	1.1
C17:0 anteiso	1.1
C14:0	0.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Deep-sea sediment at 4435 m of the Yap Trench
[Ref.: #67770]	Country	Pacific Ocean

[Ref.: #68177]	Sample type/isolated from	sediment of hadal zones
[Ref.: #68177]	Sampling date	2017-06-01
[Ref.: #68177]	Geographic location (country and/or sea, region)	West Pacific Yap Trench
[Ref.: #68177]	Country	Pacific Ocean
[Ref.: #68177]	Geographic location	-9.8986°/-138.3860°
[Ref.: #68177]	Enrichment culture	marine broth
[Ref.: #68177]	Enrichment culture duration	7 days
[Ref.: #68177]	Isolation/enrichment procedure	enriched at 60 MPa
* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Oceanobacillus sp. strain ylb-02 16S ribosomal RNA gene, partial sequence	MG913995	1428	ENA	2448030 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #68177]	Oceanobacillus piezotolerans YLB-02	GCA_003665065	contig	GenBank	2448030 tax ID

[Ref.: #67770]

GC-content

37.3 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Oceanobacillus piezotolerans YLB-02 NCBI: GCA_003665065.1
[Ref.: #69480]	motile	yes	88.08	yes
[Ref.: #69480]	flagellated	yes	82.512	no
[Ref.: #69480]	gram-positive	yes	87.766	yes
[Ref.: #69480]	anaerobic	no	98.458	yes
[Ref.: #69480]	aerobic	yes	91.561	no
[Ref.: #69480]	halophile	yes	87.022	no
[Ref.: #69480]	spore-forming	yes	91.606	yes
[Ref.: #69480]	glucose-util	yes	85.261	no
[Ref.: #69480]	thermophile	no	99.261	yes
[Ref.: #69480]	glucose-ferment	no	88.224	no

Availability in culture collections External links

[Ref.: #67770]

Culture collection no.

JCM 32870, MCCC 1A12699

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Two novel species of the family Bacillaceae: Oceanobacillus piezotolerans sp. nov. and Bacillus piezotolerans sp. nov., from deep-sea sediment samples of Yap Trench.	Yu L, Tang X, Wei S, Qiu Y, Xu X, Xu G, Wang Q, Yang Q	Int J Syst Evol Microbiol	10.1099/ijsem.0.003559	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68177

Libo Yu, Xixiang Tang, Shiping Wei, Yinkun Qiu, Xiashutong Xu, Guangxin Xu, Qilin Wang and Qian Yang: Two novel species of the family Bacillaceae: Oceanobacillus piezotolerans sp. nov. and Bacillus piezotolerans sp. nov., from deep-sea sediment samples of Yap Trench. IJSEM 69: 3022 - 3030 2019 ( DOI 10.1099/ijsem.0.003559 )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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