Clostridium fermenticellae (CICC 24501, JCM 32827, JN500901)

Name: Clostridium fermenticellae (CICC 24501, JCM 32827, JN500901)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 164372

Type strain

Strain Designation JN500901

Culture col. no. CICC 24501 JCM 32827 JN500901

NCBI tax ID(s) 2068654

Special Collections JCM Literature strains

Links

Clostridium fermenticellae JN500901 is an obligate anaerobe, spore-forming, mesophilic prokaryote that forms irregular colonies and was isolated from Mud collected from the bottom layer of a baijiu fermentation cellar located in Luzhou Laojiao Group Co., Ltd..

spore-forming Gram-positive rod-shaped colony-forming obligate anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 394535

JCM 32827

@ref 20215

Last LPSN update 2025-12-16 10:09:26

Domain Bacillati

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Clostridiaceae

Genus Clostridium

Species Clostridium fermenticellae

Full scientific name Clostridium fermenticellae Xu et al. 2019

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
67831	positive	3.5-7.5 µm	0.5-0.8 µm	rod-shaped

Colony morphology

@ref	Colony color	Colony shape	Incubation period	Medium used
67831	light yellow	irregular	5 days	RCM agar

Culture and growth conditions

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
67770	positive	growth	37	mesophilic
67831	positive	growth	22-40
67831	positive	optimum	37	mesophilic

Culture pH

@ref	Ability	Type	PH
67831	positive	optimum	5
67831	positive	growth	4.5-8

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
67831	obligate anaerobe
125439	anaerobe	99.6

Spore formation

@ref	Spore description	Type of spore	Spore formation
67831	terminal spores	spore

Halophily

@ref	Salt	Growth	Tested relation	Concentration
67831	NaCl	positive	growth	0-2 %(w/v)

Observation

@ref	Observation
67831	encapsulated and replicates by binary fission
67831	colonies giving off an unpleasant smell

Tolerance

@ref	Compound	Percentage
67831	Ethanol	0-10

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
67831	15824 ChEBI	D-fructose	+	carbon source
67831	18287 ChEBI	L-fucose	+	carbon source
67831	62345 ChEBI	L-rhamnose	+	carbon source

Isolation, sampling and environmental information

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature	Isolation procedure
67770	Mud collected from the bottom layer of a baijiu fermentation cellar located in Luzhou Laojiao Group Co., Ltd.	Luzhou City, Sichuan Province	China	CHN	Asia
67831	pit mud continuously used over 100 years (bottom layer of a baijiu fermentation cellar (length-width-height=4.3 m x 2.3 m x 2.3 m))	Luzhou Laojiao Group Co., Ltd, located in Luzhou city, Sichuan Province	China	CHN	Asia	28.8963	105.497 28.8963/105.497	reinforced clostridial medium	(pH 6.5) containing 10.0 g l-1 peptone, 10.0 g l-1 beef extract, 3.0 g l-1 yeast extract, 5.0 g l-1 glucose, 1.0 g l-1 soluble starch, 0.5 g l-1 cysteine hydrochloride, 3.0 g l-1 sodium chloride and 3.0 g l-1 sodium acetate with 1 mg l-1 resazurin	7 days	37	5 g pit mud was suspended in 100 ml sterile NaCl solution (0.85 %, w/v) using a 250 ml Erlenmeyer flask and mixed thoroughly. Before inoculating into liquid reinforced clostridial medium, the mud suspension was heated at 80°C for 10 min to kill the vegetative bacterial cells. After autoclaving at 121°C for 20 min, the RCM was kept in an anaerobic system overnight with a gas mixture, including 80 % N2, 10 % CO2 and 10% H2, to reduce oxygen in the medium. Ten millilitres of the mud suspension was incubated in 100 ml RCM and cultured anaerobically for 7 days at 37°C.

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67831	ASM360035v1 assembly for Clostridium fermenticellae JN500901	complete	GCA_003600355	2068654.3	2877458654	2068654	98.91

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
67770	Clostridium fermenticellae 16S ribosomal RNA gene, partial sequence	MG753792	1382		2068654

GC content

@ref	GC-content (mol%)	Method
67770	31	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Clostridium fermenticellae JN500901
NCBI: GCA_003600355

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	82.50	no
125439	motility	BacteriaNetⓘ	yes	80.30	no
125439	gram_stain	BacteriaNetⓘ	positive	71.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.60	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Biotechnology	Metabolite-Based Mutualistic Interaction between Two Novel Clostridial Species from Pit Mud Enhances Butyrate and Caproate Production.	Sun H, Chai LJ, Fang GY, Lu ZM, Zhang XJ, Wang ST, Shen CH, Shi JS, Xu ZH	Appl Environ Microbiol	10.1128/aem.00484-22	2022
Phylogeny	Clostridium fermenticellae sp. nov., isolated from the mud in a fermentation cellar for the production of the Chinese liquor, baijiu.	Xu PX, Chai LJ, Qiu T, Zhang XJ, Lu ZM, Xiao C, Wang ST, Shen CH, Shi JS, Xu ZH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003254	2019

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67831	Peng-Xiang Xu, Li-Juan Chai, Ting Qiu, Xiao-Juan Zhang, Zhen-Ming Lu, Chen Xiao, Song-Tao Wang, Cai-Hong Shen, Jin-Song Shi, Zheng-Hong Xu: Clostridium fermenticellae sp. nov., isolated from the mud in a fermentation cellar for the production of the Chinese liquor, baijiu. IJSEM 69: 859 - 865 2019 ( DOI 10.1099/ijsem.0.003254 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Clostridium fermenticellae (CICC 24501, JCM 32827, JN500901)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Tolerance

Metabolite utilization

Isolation, sampling and environmental information

Isolation

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_003600355

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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