Name: Clostridium fermenticellae JN500901
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 164372

Type strain:

Species: Clostridium fermenticellae

Strain Designation: JN500901

Culture col. no.: JCM 32827, CICC 24501

Strain history: Z. Xu; Jiangnan Univ., China; JN500901.

NCBI tax ID(s): 2068654 (species)

Section

Clostridium fermenticellae JN500901 is an obligate anaerobe, spore-forming, mesophilic bacterium that forms irregular colonies and was isolated from Mud collected from the bottom layer of a baijiu fermentation cellar located in Luzhou Laojiao Group Co., Ltd..

colony-forming
Gram-positive
rod-shaped
spore-forming
mesophilic
obligate anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Clostridium*
	Species	**Clostridium fermenticellae**
	Full Scientific Name (LPSN)	Clostridium fermenticellae Xu et al. 2019

Information on morphological and physiological properties Morphology

[Ref.: #67831]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %99.725

[Ref.: #67831]	Cell length	3.5-7.5 µm

[Ref.: #67831]	Cell width	0.5-0.8 µm

[Ref.: #67831]	Cell shape	rod-shaped

[Ref.: #67831]	Motility	no

[Ref.: #67831]	Colony color	light yellow
[Ref.: #67831]	Colony shape	irregular
[Ref.: #67831]	Incubation period	5 days
[Ref.: #67831]	Cultivation medium used	RCM agar

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #67770]	growth	37 °C
[Ref.: #67831]	growth	22-40 °C
[Ref.: #67831]	optimum	37 °C

[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67831]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #67831]			optimum	5
[Ref.: #67831]			growth	4.5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67831]	Oxygen tolerance	obligate anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %100

[Ref.: #67831]	Spore description	terminal spores
[Ref.: #67831]	Type of spore	spore
[Ref.: #67831]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.447
[Ref.: #69481]	Ability of spore formation	yes Confidence in %97

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #67831]		NaCl		growth	0-2	%(w/v)

[Ref.: #67831]	Observation	encapsulated and replicates by binary fission
[Ref.: #67831]	Observation	colonies giving off an unpleasant smell

[Ref.: #67831]	Name of tolerated compound	Ethanol
[Ref.: #67831]	Tolerance percentage	0-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #67831]	D-fructose ChEBI	+	carbon source
[Ref.: #67831]	L-fucose ChEBI	+	carbon source
[Ref.: #67831]	L-rhamnose ChEBI	+	carbon source

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Mud collected from the bottom layer of a baijiu fermentation cellar located in Luzhou Laojiao Group Co., Ltd.
[Ref.: #67770]	Geographic location (country and/or sea, region)	Luzhou City, Sichuan Province
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #67831]	Sample type/isolated from	pit mud continuously used over 100 years (bottom layer of a baijiu fermentation cellar (length-width-height=4.3 m x 2.3 m x 2.3 m))
[Ref.: #67831]	Geographic location (country and/or sea, region)	Luzhou Laojiao Group Co., Ltd, located in Luzhou city, Sichuan Province
[Ref.: #67831]	Country	China
[Ref.: #67831]	Country ISO 3 Code	CHN
[Ref.: #67831]	Continent	Asia
[Ref.: #67831]	Geographic location	28.8963°/105.4970°
[Ref.: #67831]	Enrichment culture	reinforced clostridial medium
[Ref.: #67831]	Enrichment culture composition	(pH 6.5) containing 10.0 g l-1 peptone, 10.0 g l-1 beef extract, 3.0 g l-1 yeast extract, 5.0 g l-1 glucose, 1.0 g l-1 soluble starch, 0.5 g l-1 cysteine hydrochloride, 3.0 g l-1 sodium chloride and 3.0 g l-1 sodium acetate with 1 mg l-1 resazurin
[Ref.: #67831]	Enrichment culture duration	7 days
[Ref.: #67831]	Enrichment culture temperature	37 ̊C
[Ref.: #67831]	Isolation/enrichment procedure	5 g pit mud was suspended in 100 ml sterile NaCl solution (0.85 %, w/v) using a 250 ml Erlenmeyer flask and mixed thoroughly. Before inoculating into liquid reinforced clostridial medium, the mud suspension was heated at 80°C for 10 min to kill the vegetative bacterial cells. After autoclaving at 121°C for 20 min, the RCM was kept in an anaerobic system overnight with a gas mixture, including 80 % N2, 10 % CO2 and 10% H2, to reduce oxygen in the medium. Ten millilitres of the mud suspension was incubated in 100 ml RCM and cultured anaerobically for 7 days at 37°C.
* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Clostridium fermenticellae 16S ribosomal RNA gene, partial sequence	MG753792	1382	ENA	2068654 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67831]	Clostridium fermenticellae JN500901	GCA_003600355	complete	GenBank	2068654 tax ID
[Ref.: #66792]	Clostridium fermenticellae JN500901	2877458654	complete	JGI IMG/M	2068654 tax ID	*

[Ref.: #67770]

GC-content

31 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Clostridium fermenticellae JN500901 NCBI: GCA_003600355.1
[Ref.: #69481]	spore-forming	yes	97	no

Availability in culture collections External links

[Ref.: #67770]

Culture collection no.

JCM 32827, CICC 24501

Literature:

Topic	Title	Authors	Journal	DOI	Year
Biotechnology	Metabolite-Based Mutualistic Interaction between Two Novel Clostridial Species from Pit Mud Enhances Butyrate and Caproate Production.	Sun H, Chai LJ, Fang GY, Lu ZM, Zhang XJ, Wang ST, Shen CH, Shi JS, Xu ZH	Appl Environ Microbiol	10.1128/aem.00484-22	2022	*
Phylogeny	Clostridium fermenticellae sp. nov., isolated from the mud in a fermentation cellar for the production of the Chinese liquor, baijiu.	Xu PX, Chai LJ, Qiu T, Zhang XJ, Lu ZM, Xiao C, Wang ST, Shen CH, Shi JS, Xu ZH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003254	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#67831

Peng-Xiang Xu, Li-Juan Chai, Ting Qiu, Xiao-Juan Zhang, Zhen-Ming Lu, Chen Xiao, Song-Tao Wang, Cai-Hong Shen, Jin-Song Shi, Zheng-Hong Xu: Clostridium fermenticellae sp. nov., isolated from the mud in a fermentation cellar for the production of the Chinese liquor, baijiu. IJSEM 69: 859 - 865 2019 ( DOI 10.1099/ijsem.0.003254 )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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