Name: Clostridium thermarum DSM 100530
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 160635

Type strain:

Species: Clostridium thermarum

Culture col. no.: DSM 100530, YIM A18, KCTC 15658, SYSU GA15002

Strain history: <- W.-J. Li, Yunnan Univ., Yunnan Inst. Microbiol. (YIM), Kunming, China; SYSU GA15002 <- L. Liu

NCBI tax ID(s): 1716543 (species)

SI-ID 403594

DSM 100530

Section

Clostridium thermarum DSM 100530 is an obligate aerobe, spore-forming, thermophilic bacterium that was isolated from water-sludge mixture of hot spring.

Gram-positive
motile
rod-shaped
spore-forming
obligate aerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Clostridium*
	Species	**Clostridium thermarum**
	Full Scientific Name (LPSN)	Clostridium thermarum Liu et al. 2021

Information on morphological and physiological properties Morphology

[Ref.: #69625]	Gram stain	positive

[Ref.: #69625]	Cell shape	rod-shaped

[Ref.: #69625]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #67544]

Culture medium

RCM MEDIUM (DSMZ Medium 634)

[Ref.: #67544]

Culture medium growth

[Ref.: #67544]

Culture medium link

Medium recipe at MediaDive

[Ref.: #67544]

Culture medium composition

expand / minimize

[Ref.: #69625]

Culture medium

reinforced clostridial medium (RCM)

[Ref.: #69625]

Culture medium growth

		Temperature
[Ref.: #67544]	growth	45 °C
[Ref.: #69625]	growth	28-50 °C
[Ref.: #69625]	optimum	45 °C

[Ref.: #67544]	Temperature range	thermophilic
[Ref.: #69625]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #69625]			optimum	7
[Ref.: #69625]			growth	6-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69625]

Oxygen tolerance

obligate aerobe

[Ref.: #69625]	Type of spore	spore
[Ref.: #69625]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69625]		NaCl		growth	0-1	%(w/v)

[Ref.: #69625]

Observation

lignocellulose biodegradation

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69625]	carboxymethylcellulose ChEBI	+	hydrolysis
[Ref.: #69625]	cellobiose ChEBI	+	builds acid from
[Ref.: #69625]	cellulose ChEBI	+	hydrolysis
[Ref.: #69625]	D-arabinose ChEBI	-	carbon source
[Ref.: #69625]	D-mannitol ChEBI	+	assimilation
[Ref.: #69625]	D-ribose ChEBI	-	assimilation
[Ref.: #69625]	D-xylose ChEBI	+	assimilation
[Ref.: #69625]	esculin ChEBI	+	hydrolysis
[Ref.: #69625]	gelatin ChEBI	+	hydrolysis
[Ref.: #69625]	glucose ChEBI	+	builds acid from
[Ref.: #69625]	glycerol ChEBI	+	builds acid from
[Ref.: #69625]	glycerol ChEBI	+	hydrolysis
[Ref.: #69625]	L-arabinose ChEBI	+	assimilation
[Ref.: #69625]	mannitol ChEBI	+	builds acid from
[Ref.: #69625]	myo-inositol ChEBI	+	assimilation
[Ref.: #69625]	raffinose ChEBI	-	assimilation
[Ref.: #69625]	starch ChEBI	+	hydrolysis
[Ref.: #69625]	xylan ChEBI	+	hydrolysis
[Ref.: #69625]	xylose ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #69625]		ethanol ChEBI	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #69625]	alanine arylamidase	+	3.4.11.2
[Ref.: #69625]	alpha-arabinosidase	+
[Ref.: #69625]	alpha-galactosidase	-	3.2.1.22
[Ref.: #69625]	alpha-glucosidase	-	3.2.1.20
[Ref.: #69625]	arginine arylamidase	+
[Ref.: #69625]	arginine dihydrolase	-	3.5.3.6
[Ref.: #69625]	beta-glucosidase	+	3.2.1.21
[Ref.: #69625]	glutamyl-glutamate arylamidase	+
[Ref.: #69625]	glycin arylamidase	+
[Ref.: #69625]	histidine arylamidase	+
[Ref.: #69625]	leucine arylamidase	-	3.4.11.1
[Ref.: #69625]	leucyl glycin arylamidase	+	3.4.11.1
[Ref.: #69625]	phenylalanine arylamidase	-
[Ref.: #69625]	proline-arylamidase	+	3.4.11.5
[Ref.: #69625]	serine arylamidase	-
[Ref.: #69625]	tyrosine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67544]	Sample type/isolated from	water-sludge mixture of hot spring
[Ref.: #67544]	Geographic location (country and/or sea, region)	Yunnan province
[Ref.: #67544]	Country	China
[Ref.: #67544]	Country ISO 3 Code	CHN
[Ref.: #67544]	Continent	Asia

[Ref.: #69625]	Sample type/isolated from	hot spring
[Ref.: #69625]	Sampling date	2016-01-02
[Ref.: #69625]	Geographic location (country and/or sea, region)	Yunnan province
[Ref.: #69625]	Country	China
[Ref.: #69625]	Country ISO 3 Code	CHN
[Ref.: #69625]	Continent	Asia
[Ref.: #69625]	Geographic location	25.4411°/98.4600°
[Ref.: #69625]	Enrichment culture	2 × enrichment medium
[Ref.: #69625]	Enrichment culture composition	see papers materials and methods section
[Ref.: #69625]	Enrichment culture duration	2 days
[Ref.: #69625]	Isolation date	2016-09-20
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence KR052807 (>99% sequence identity) for Clostridium thermarum subclade from Microbeatlas

Aquatic Samples	455
Soil Samples	1523
Animal Samples	376
Plant Samples	156
Total Samples	2510

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67544]	Clostridium sp. SYSU GA15002 16S ribosomal RNA gene, partial sequence	KR052807	1510	ENA	1716543 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #69625]	Clostridium thermarum SYSU GA15002	GCA_006351925	complete	GenBank	1716543 tax ID
[Ref.: #69625]	Whole genome shotgun	CP040924		GenBank	1716543 tax ID

[Ref.: #67544]	GC-content	36.6 mol%	sequence analysis
[Ref.: #69625]	GC-content	36.6 mol%	genome sequence analysis

Availability in culture collections External links

[Ref.: #67544]

Culture collection no.

DSM 100530, YIM A18, KCTC 15658, SYSU GA15002

[Ref.: #112873]

SI-ID 403594

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Isolation of Clostridium from Yunnan-Tibet hot springs and description of Clostridium thermarum sp. nov. with lignocellulosic ethanol production.	Liu L, Jiao JY, Fang BZ, Lv AP, Ming YZ, Li MM, Salam N, Li WJ	Syst Appl Microbiol	10.1016/j.syapm.2020.126104	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#67544

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100530

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69625

Lan Liu, Jian-Yu Jiao, Bao-Zhu Fang, Ai-Ping Lv, Yu-Zhen Ming, Meng-Meng Li, Nimaichand Salam, Wen-Jun Li: Isolation of Clostridium from Yunnan-Tibet hot springs and description of Clostridium thermarum sp. nov. with lignocellulosic ethanol production. Syst Appl Microbiol. 43: 2020 ( DOI 10.1016/j.syapm.2020.126104 )

#112873

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403594.1 )

* These data were automatically processed and therefore are not curated

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