Name: Kushneria phyllosphaerae EAod3
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 159726

Type strain:

Species: Kushneria phyllosphaerae

Strain Designation: EAod3

Culture col. no.: CECT 9073, LMG 29856

NCBI tax ID(s): 2100822 (species)

Special Collections

Literature strains

Section

When using BacDive for research please cite our paper

Kushneria phyllosphaerae EAod3 is an aerobe, moderately halophilic, mesophilic bacterium that forms irregular colonies and was isolated from from the aerial part of Arthrocnemum macrostachyum.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
mesophilic
moderately halophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Oceanospirillales*
	Family	*Halomonadaceae*
	Genus	*Kushneria*
	Species	**Kushneria phyllosphaerae**
	Full Scientific Name (LPSN)	Kushneria phyllosphaerae Navarro-Torre et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66633]	Gram stain	negative
[Ref.: #125439]	Gram stain	negative Confidence in %99.9

[Ref.: #66633]	Cell length	0.2-0.3 µm

[Ref.: #66633]	Cell width	0.1 µm

[Ref.: #66633]	Cell shape	rod-shaped

[Ref.: #66633]	Motility	yes

[Ref.: #66633]	Colony size	3 mm
[Ref.: #66633]	Colony color	light salmon (RAL 060 80 40)
[Ref.: #66633]	Colony shape	irregular
[Ref.: #66633]	Incubation period	2 days
[Ref.: #66633]	Cultivation medium used	Tryptic Soy Agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66633]

Culture medium

MacConkey agar

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Culture medium growth

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Culture medium

Marine agar (MA) 2216

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Culture medium growth

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Culture medium

tryptic soy agar (TSA)

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

cetrimide agar

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Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #66633]			optimum	30 °C
[Ref.: #66633]			growth	4-37 °C

	pH	Kind of pH		pH
[Ref.: #66633]			optimum	7
[Ref.: #66633]			growth	5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66633]	Oxygen tolerance	aerobe
[Ref.: #125439]	Oxygen tolerance	aerobe Confidence in %94.5

[Ref.: #66633]	Ability of spore formation	no
[Ref.: #125439]	Ability of spore formation	no Confidence in %99.7

[Ref.: #66633]

Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66633]		NaCl		growth	0.5-25	%(w/v)
[Ref.: #66633]		NaCl		minimum	0.5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66633]	(S)-malic acid ChEBI	+	oxidation
[Ref.: #66633]	4-hydroxyphenylacetic acid ChEBI	+	oxidation
[Ref.: #66633]	adipate ChEBI	-	assimilation
[Ref.: #66633]	butyrate ChEBI	+	oxidation
[Ref.: #66633]	casein	+	hydrolysis
[Ref.: #66633]	cellulose ChEBI	-	hydrolysis
[Ref.: #66633]	chitin ChEBI	-	hydrolysis
[Ref.: #66633]	citric acid ChEBI	+	oxidation
[Ref.: #66633]	D-arabitol ChEBI	+	oxidation
[Ref.: #66633]	D-fructose ChEBI	+	oxidation
[Ref.: #66633]	D-fucose ChEBI	+	oxidation
[Ref.: #66633]	D-galactose ChEBI	+	oxidation
[Ref.: #66633]	D-galacturonic acid ChEBI	+	oxidation
[Ref.: #66633]	D-glucarate ChEBI	+	oxidation
[Ref.: #66633]	D-gluconate ChEBI	+	oxidation
[Ref.: #66633]	D-glucose ChEBI	-	assimilation
[Ref.: #66633]	D-glucose ChEBI	+	fermentation
[Ref.: #66633]	D-glucose ChEBI	+	oxidation
[Ref.: #66633]	D-glucuronate ChEBI	+	oxidation
[Ref.: #66633]	D-mannitol ChEBI	-	assimilation
[Ref.: #66633]	D-mannitol ChEBI	-	builds acid from
[Ref.: #66633]	D-mannose ChEBI	-	assimilation
[Ref.: #66633]	D-ribose ChEBI	-	builds acid from
[Ref.: #66633]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #66633]	decanoate ChEBI	-	assimilation
[Ref.: #66633]	dna ChEBI	+	hydrolysis
[Ref.: #66633]	esculin ChEBI	+	hydrolysis
[Ref.: #66633]	galactarate ChEBI	+	oxidation
[Ref.: #66633]	gamma-aminobutyric acid ChEBI	+	oxidation
[Ref.: #66633]	gelatin ChEBI	-	hydrolysis
[Ref.: #66633]	glucuronamide ChEBI	+	oxidation
[Ref.: #66633]	glycerol ChEBI	+	oxidation
[Ref.: #66633]	glycogen ChEBI	-	builds acid from
[Ref.: #66633]	inosine ChEBI	+	oxidation
[Ref.: #66633]	inulin ChEBI	-	builds acid from
[Ref.: #66633]	L-arabinose ChEBI	-	assimilation
[Ref.: #66633]	L-arabinose ChEBI	-	builds acid from
[Ref.: #66633]	L-galactonic acid gamma-lactone ChEBI	+	oxidation
[Ref.: #66633]	L-glutamate ChEBI	+	oxidation
[Ref.: #66633]	L-serine ChEBI	+	oxidation
[Ref.: #66633]	lactose ChEBI	-	builds acid from
[Ref.: #66633]	malate ChEBI	+	assimilation
[Ref.: #66633]	maltose ChEBI	-	assimilation
[Ref.: #66633]	maltose ChEBI	+	oxidation
[Ref.: #66633]	methyl pyruvate ChEBI	+	oxidation
[Ref.: #66633]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #66633]	nitrate ChEBI	+	reduction
[Ref.: #66633]	pectin ChEBI	-	hydrolysis
[Ref.: #66633]	pectin ChEBI	+	oxidation
[Ref.: #66633]	phenylacetate ChEBI	-	assimilation
[Ref.: #66633]	potassium gluconate ChEBI	+	assimilation
[Ref.: #66633]	raffinose ChEBI	-	builds acid from
[Ref.: #66633]	sodium citrate ChEBI	-	assimilation
[Ref.: #66633]	sodium formate ChEBI	+	oxidation
[Ref.: #66633]	sodium lactate ChEBI	+	oxidation
[Ref.: #66633]	starch ChEBI	-	builds acid from
[Ref.: #66633]	starch ChEBI	+	hydrolysis
[Ref.: #66633]	sucrose ChEBI	+	oxidation
[Ref.: #66633]	trehalose ChEBI	+	oxidation
[Ref.: #66633]	turanose ChEBI	+	oxidation
[Ref.: #66633]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #66633]		acetoin ChEBI	yes
[Ref.: #66633]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #66633]		acetoin ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #66633]	acid phosphatase	+	3.1.3.2
[Ref.: #66633]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66633]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #66633]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66633]	alpha-galactosidase	-	3.2.1.22
[Ref.: #66633]	alpha-glucosidase	+	3.2.1.20
[Ref.: #66633]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66633]	arginine dihydrolase	-	3.5.3.6
[Ref.: #66633]	beta-galactosidase	+	3.2.1.23
[Ref.: #66633]	beta-glucosidase	+	3.2.1.21
[Ref.: #66633]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66633]	catalase	+	1.11.1.6
[Ref.: #66633]	cystine arylamidase	-	3.4.11.3
[Ref.: #66633]	cytochrome oxidase	-	1.9.3.1
[Ref.: #66633]	esterase (C 4)	+
[Ref.: #66633]	esterase Lipase (C 8)	+
[Ref.: #66633]	leucine arylamidase	+	3.4.11.1
[Ref.: #66633]	lipase (C 14)	+
[Ref.: #66633]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #66633]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66633]	pyrrolidonyl arylamidase	-	3.4.19.3
[Ref.: #66633]	trypsin	-	3.4.21.4
[Ref.: #66633]	urease	-	3.5.1.5
[Ref.: #66633]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66633]

Fatty acid	Percentage
C18:1 w7c and/or C18:1 w6c	44.8
C16:0	26.4
C16:1 w7c and/or C16:1 w6c	13.7
C12:0 3OH	9.2
C12:0	2.3
C12:0 2OH	1.4

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66633]	Sample type/isolated from	from the aerial part of Arthrocnemum macrostachyum
[Ref.: #66633]	Geographic location (country and/or sea, region)	Odiel marshes in Huelva
[Ref.: #66633]	Country	Spain
[Ref.: #66633]	Country ISO 3 Code	ESP
[Ref.: #66633]	Continent	Europe
[Ref.: #66633]	Geographic location	37.2166°/-6.9500°
[Ref.: #66633]	Enrichment culture	tryptic soy agar
[Ref.: #66633]	Enrichment culture composition	supplemented with 0.3 M NaCl
[Ref.: #66633]	Enrichment culture duration	3 days
[Ref.: #66633]	Enrichment culture temperature	28 ̊C
* marker position based on {}

Isolation sources categories

#Host

#Plants

#Shrub (Scrub)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66633]	Kushneria phyllosphaerae strain EAod3 16S ribosomal RNA gene, partial sequence	KU320856	1467	GenBank ENA	2100822 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Kushneria phyllosphaerae EAod3	GCA_900312995	contig	GenBank ENA	2100822 tax ID	*
[Ref.: #66792]	Kushneria phyllosphaerae EAod3	2839383563	draft	JGI IMG/M	2100822 tax ID	*

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GC-content

59.3 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Kushneria phyllosphaerae EAod3 NCBI: GCA_900312995
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	99.7
[Ref.: #125439]	BacteriaNet	motilityⓘ	yes	89.5
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	negative	99.9
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	aerobe	94.5

Availability in culture collections External links

[Ref.: #66633]

Culture collection no.

CECT 9073, LMG 29856

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Kushneria phyllosphaerae sp. nov. and Kushneria endophytica sp. nov., plant growth promoting endophytes isolated from the halophyte plant Arthrocnemum macrostachyum.	Navarro-Torre S, Carro L, Rodriguez-Llorente ID, Pajuelo E, Caviedes MA, Igual JM, Redondo-Gomez S, Camacho M, Klenk HP, Montero-Calasanz MDC	Int J Syst Evol Microbiol	10.1099/ijsem.0.002897	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66633

Salvadora Navarro-Torre, Lorena Carro, Ignacio D. Rodriguez-Llorente, Eloisa Pajuelo, Miguel Angel Caviedes, Jose Mariano Igual, Susana Redondo-Gomez, Maria Camacho, Hans-Peter Klenk, Maria del Carmen Montero-Calasanz: Kushneria phyllosphaerae sp. nov. and Kushneria endophytica sp. nov., plant growth promoting endophytes isolated from the halophyte plant Arthrocnemum macrostachyum. IJSEM 68: 2800 - 2806 2018 ( DOI 10.1099/ijsem.0.002897 , PubMed 30010522 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

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