Name: Propioniciclava flava WS 4900
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159196

Type strain:

Species: Propioniciclava flava

Strain Designation: WS 4900, VG341

Culture col. no.: DSM 100885, LMG 29089, WS 4900

Strain history: <- M. Wenning, TU Munich, Freising-Weihenstephan, Germany; WS4900 <- F. Breitenwieser

NCBI tax ID(s): 2072026 (species)

SI-ID 397841

DSM 100885

Section

Propioniciclava flava WS 4900 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from Raw milk.

Gram-positive
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Propionibacteriaceae*
	Genus	*Propioniciclava*
	Species	**Propioniciclava flava**
	Full Scientific Name (LPSN)	Propioniciclava flava (Wenning et al. 2020) Li et al. 2022

Synonym

Brevilactibacter flavus

Information on morphological and physiological properties Morphology

[Ref.: #69584]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69584]	Cell length	0.8-2.2 µm

[Ref.: #69584]	Cell width	0.4-0.9 mm

[Ref.: #69584]	Cell shape	rod-shaped

[Ref.: #69584]	Motility	no

[Ref.: #69584]	Colony color	yellow to orange pigmented or white
[Ref.: #69584]	Incubation period	3-5 days
[Ref.: #69584]	Cultivation medium used	Trypticase Soy Agar (TSA)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66019]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #66019]

Culture medium growth

[Ref.: #66019]

Culture medium link

Medium recipe at MediaDive

[Ref.: #66019]

Culture medium composition

expand / minimize

[Ref.: #69584]

Culture medium

Trypticase Soy Agar (TSA)

[Ref.: #69584]

Culture medium growth

		Temperature
[Ref.: #66019]	growth	30 °C
[Ref.: #69584]	growth	6-40 °C
[Ref.: #69584]	optimum	30-34 °C

[Ref.: #66019]	Temperature range	mesophilic
[Ref.: #69584]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #69584]			optimum	7.5
[Ref.: #69584]			growth	5.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69584]

Oxygen tolerance

anaerobe

[Ref.: #69584]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.941
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69584]		NaCl		growth	5	%
[Ref.: #69584]		NaCl		optimum	1	%(w/v)

[Ref.: #69584]

Observation

The polar lipid profile is composed of the major compounds diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids (GL1-3) and minor amounts of four unidentified glycolipids (GL4-7) and six unindentified lipids lacking a functional group (L1-6).

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69584]	acetate ChEBI	+	anaerobic catabolization
[Ref.: #69584]	arbutin ChEBI	+	builds acid from
[Ref.: #69584]	beta-D-xylose ChEBI	+/-	builds acid from
[Ref.: #69584]	cellobiose ChEBI	+/-	builds acid from
[Ref.: #69584]	D-arabitol ChEBI	+	builds acid from
[Ref.: #69584]	D-fructose ChEBI	+	builds acid from
[Ref.: #69584]	D-galactose ChEBI	+	builds acid from
[Ref.: #69584]	D-glucose ChEBI	+	builds acid from
[Ref.: #69584]	D-mannitol ChEBI	+	builds acid from
[Ref.: #69584]	D-mannose ChEBI	+	builds acid from
[Ref.: #69584]	D-ribose ChEBI	+	builds acid from
[Ref.: #69584]	D-xylose ChEBI	+	builds acid from
[Ref.: #69584]	esculin ChEBI	+	hydrolysis
[Ref.: #69584]	formate ChEBI	+	anaerobic catabolization
[Ref.: #69584]	gelatin ChEBI	-	hydrolysis
[Ref.: #69584]	glycerol ChEBI	+	builds acid from
[Ref.: #69584]	indole ChEBI	-	fermentation
[Ref.: #69584]	lactate ChEBI	+	anaerobic catabolization
[Ref.: #69584]	lactose ChEBI	+	builds acid from
[Ref.: #69584]	maltose ChEBI	+	builds acid from
[Ref.: #69584]	melezitose ChEBI	+	builds acid from
[Ref.: #69584]	melibiose ChEBI	+	builds acid from
[Ref.: #69584]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #69584]	N-acetylglucosamine ChEBI	+/-	builds acid from
[Ref.: #69584]	nitrate ChEBI	+/-	reduction
[Ref.: #69584]	propionate ChEBI	+	anaerobic catabolization
[Ref.: #69584]	raffinose ChEBI	+/-	builds acid from
[Ref.: #69584]	salicin ChEBI	+	builds acid from
[Ref.: #69584]	starch ChEBI	-	hydrolysis
[Ref.: #69584]	succinate ChEBI	+	anaerobic catabolization
[Ref.: #69584]	sucrose ChEBI	+	builds acid from
[Ref.: #69584]	trehalose ChEBI	+	builds acid from
[Ref.: #69584]	turanose ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #69584]		hydrogen sulfide ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #69584]	arginine dihydrolase	-	3.5.3.6
[Ref.: #69584]	beta-galactosidase	+	3.2.1.23
[Ref.: #69584]	catalase	+	1.11.1.6
[Ref.: #69584]	cytochrome oxidase	+	1.9.3.1
[Ref.: #69584]	urease	-	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #69584]

Fatty acid	Percentage
C15:0 anteiso	54
C16:0	8
C16:0 N alcohol	8
C17:0	6
C17:0 anteiso	5
C18:2 w6,9c / C18:0 anteiso	3

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66019]	Sample type/isolated from	Raw milk
[Ref.: #66019]	Geographic location (country and/or sea, region)	Röttingen
[Ref.: #66019]	Country	Germany
[Ref.: #66019]	Country ISO 3 Code	DEU
[Ref.: #66019]	Continent	Europe

[Ref.: #69584]	Sample type/isolated from	was raw cow milk.
[Ref.: #69584]	Geographic location (country and/or sea, region)	southern Germany
[Ref.: #69584]	Country	Germany
[Ref.: #69584]	Country ISO 3 Code	DEU
[Ref.: #69584]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host Body Product	#Fluids	#Milk
#Host	#Mammals	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66019]	Brevilactibacter flavus strain VG341 16S ribosomal RNA gene, partial sequence	MG799849	1366	ENA	2072026 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #69584]	Propioniciclava flava VG341	GCA_004126535	contig	GenBank	2072026 tax ID
[Ref.: #69584]	genome sequence	PPCV00000000		GenBank	2072026 tax ID
[Ref.: #66792]	Propionibacteriaceae bacterium VG341	2072026.3	wgs	PATRIC	2072026 tax ID	*

[Ref.: #69584]

GC-content

67.7 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Propionibacteriaceae bacterium VG341 PATRIC: 2072026.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Propionibacteriaceae bacterium VG341 PATRIC: 2072026.3
[Ref.: #69480]	motile	no	93.566	no
[Ref.: #69480]	gram-positive	yes	91.872	no
[Ref.: #69480]	anaerobic	no	96.955	no
[Ref.: #69480]	aerobic	yes	51.125	no
[Ref.: #69480]	halophile	no	88.848	no
[Ref.: #69480]	spore-forming	no	92.03	no
[Ref.: #69480]	glucose-util	yes	89.139	no
[Ref.: #69480]	flagellated	no	98.577	no
[Ref.: #69480]	thermophile	no	96.888	yes
[Ref.: #69480]	glucose-ferment	no	59.605	no

Availability in culture collections External links

[Ref.: #66019]

Culture collection no.

DSM 100885, LMG 29089, WS 4900

[Ref.: #111697]

SI-ID 397841

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brevilactibacter flavus gen. nov., sp. nov., a novel bacterium of the family Propionibacteriaceae isolated from raw milk and dairy products and reclassification of Propioniciclava sinopodophylli as Brevilactibacter sinopodophylli comb. nov.	Wenning M, Breitenwieser F, Huptas C, Doll E, Bachler B, Schulz A, Dunkel A, Hofmann T, von Neubeck M, Busse HJ, Scherer S	Int J Syst Evol Microbiol	10.1099/ijsem.0.003909	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66019

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100885

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#69584

Mareike Wenning, Franziska Breitenwieser, Christopher Huptas, Etienne Doll, Benedikt Bächler, Annika Schulz, Andreas Dunkel, Thomas Hofmann, Mario von Neubeck, Hans-Jürgen Busse and Siegfried Scherer: Brevilactibacter flavus gen. nov., sp. nov., a novel bacterium of the family Propionibacteriaceae isolated from raw milk and dairy products and reclassification of Propioniciclava sinopodophylli as Brevilactibacter sinopodophylli comb. nov.. IJSEM 70: 2020 ( DOI 10.1099/ijsem.0.003909 )

#111697

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397841.1 )

* These data were automatically processed and therefore are not curated

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Propionibacteriaceae bacterium VG341

Propionibacteriaceae bacterium VG341

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