Name: Nonomuraea longispora KC201
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 159166

Type strain:

Species: Nonomuraea longispora

Strain Designation: KC201

Culture col. no.: DSM 102917, CGMCC 4.7339, KCTC 39781

Strain history: <- Nevzat Sahin

NCBI tax ID(s): 1848320 (species)

SI-ID 399093

DSM 102917

Special Collections

Section

Nonomuraea longispora KC201 is an aerobe, Gram-positive bacterium that builds an aerial mycelium and was isolated from desert soil.

Gram-positive
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Streptosporangiaceae*
	Genus	*Nonomuraea*
	Species	**Nonomuraea longispora**
	Full Scientific Name (LPSN)	Nonomuraea longispora Saygin et al. 2020

Information on morphological and physiological properties Morphology

[Ref.: #69554]	Gram stain	positive

[Ref.: #69554]	Motility	no

[Ref.: #69263]	Colony color	Clay brown (8003)
[Ref.: #69263]	Cultivation medium used	suter with tyrosine

[Ref.: #69263]	Colony color	Lemon yellow (1012)
[Ref.: #69263]	Cultivation medium used	ISP 3

[Ref.: #69263]	Colony color	Light ivory (1015)
[Ref.: #69263]	Cultivation medium used	ISP 4

[Ref.: #69263]	Colony color	Ochre brown (8001)
[Ref.: #69263]	Cultivation medium used	suter without tyrosine

[Ref.: #69263]	Colony color	Ochre yellow (1024)
[Ref.: #69263]	Cultivation medium used	ISP 6

[Ref.: #69263]	Colony color	Ochre yellow (1024), black red (3007), purple red (3004)
[Ref.: #69263]	Cultivation medium used	ISP 2

[Ref.: #69263]	Colony color	Ochre yellow (1024), oxide red (3009), fawn brown (8007)
[Ref.: #69263]	Cultivation medium used	ISP 5

[Ref.: #69263]	Colony color	Pale brown (8025)
[Ref.: #69263]	Cultivation medium used	ISP 7

[Ref.: #69263]	Pigment production	no
[Ref.: #69263]	Pigment name	Melanin
[Ref.: #69263]	Pigment production	no
[Ref.: #69263]	Pigment name	soluble pigment

[Ref.: #69263]	Multicellular complex forming ability	yes
[Ref.: #69263]	Multicellular complex name	Aerial mycelium
[Ref.: #69263]	Multicellular complex color	Signal white (9003)
[Ref.: #69263]	Medium Name (multicellularity)	ISP 2

[Ref.: #69263]	Multicellular complex forming ability	yes
[Ref.: #69263]	Multicellular complex name	Aerial mycelium
[Ref.: #69263]	Multicellular complex color	Signal white (9003)
[Ref.: #69263]	Medium Name (multicellularity)	ISP 3

[Ref.: #69263]	Multicellular complex forming ability	no
[Ref.: #69263]	Multicellular complex name	Aerial mycelium
[Ref.: #69263]	Medium Name (multicellularity)	ISP 4

[Ref.: #69263]	Multicellular complex forming ability	no
[Ref.: #69263]	Multicellular complex name	Aerial mycelium
[Ref.: #69263]	Medium Name (multicellularity)	ISP 5

[Ref.: #69263]	Multicellular complex forming ability	no
[Ref.: #69263]	Multicellular complex name	Aerial mycelium
[Ref.: #69263]	Medium Name (multicellularity)	ISP 6

[Ref.: #69263]	Multicellular complex forming ability	yes
[Ref.: #69263]	Multicellular complex name	Aerial mycelium
[Ref.: #69263]	Multicellular complex color	Signal white (9003)
[Ref.: #69263]	Medium Name (multicellularity)	ISP 7

[Ref.: #69263]	Multicellular complex forming ability	no
[Ref.: #69263]	Multicellular complex name	Aerial mycelium
[Ref.: #69263]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69263]	Multicellular complex forming ability	no
[Ref.: #69263]	Multicellular complex name	Aerial mycelium
[Ref.: #69263]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

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Caption:

Medium 65 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

N-Z-AMINE-MEDIUM (DSMZ Medium 554)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #65989]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #65989]	growth	28 °C
[Ref.: #67771]	growth	28 °C
[Ref.: #69554]	growth	20-37 °C
[Ref.: #69554]	optimum	28 °C

	pH	Kind of pH		pH
[Ref.: #69554]			optimum	7
[Ref.: #69554]			growth	6.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]

Oxygen tolerance

aerobe

	Salt		Salt conc.
[Ref.: #69263]	NaCl	growth	0-2.5	%
[Ref.: #69554]	NaCl	growth	0-3	%(w/v)
[Ref.: #69554]	NaCl	growth	0-4	%(w/v)

[Ref.: #69554]	Observation	The polar lipids profile includes diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, dihydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, glycophospholipids, phospholipids, a glycolipid and lipids.
[Ref.: #69554]	Observation	Whole-cell hydrolysates contain meso-A 2pm, glucose, mannose, madurose and ribose, and a trace of galactose.
[Ref.: #69554]	Observation	Good growth occurs on ISP 2, modified Bennett's, Czapek's, tryptic soy and nutrient agar, moderate growth on ISP 4, 5 and 7 agar and weak growth on ISP 6 agar substrate mycelia may be brown or cream, not produce diffusible or melanoid pigments.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69554]	4-hydroxy-L-proline ChEBI	+	nitrogen source
[Ref.: #69554]	adenine ChEBI	-	degradation
[Ref.: #69554]	allantoin ChEBI	-	hydrolysis
[Ref.: #69263]	arabinose ChEBI	+/-	growth
[Ref.: #69554]	arbutin ChEBI	+	hydrolysis
[Ref.: #69554]	casein	-	degradation
[Ref.: #69554]	cellobiose ChEBI	+	carbon source
[Ref.: #69554]	cellobiose ChEBI	+	energy source
[Ref.: #69263]	cellulose ChEBI	+/-	growth
[Ref.: #69554]	chitin ChEBI	-	degradation
[Ref.: #69554]	D-fructose ChEBI	-	carbon source
[Ref.: #69554]	D-fructose ChEBI	-	energy source
[Ref.: #69554]	D-galactose ChEBI	-	carbon source
[Ref.: #69554]	D-galactose ChEBI	-	energy source
[Ref.: #69554]	D-glucose ChEBI	+	carbon source
[Ref.: #69554]	D-glucose ChEBI	+	energy source
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69554]	D-mannitol ChEBI	+	carbon source
[Ref.: #69554]	D-mannitol ChEBI	+	energy source
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #69554]	D-mannose ChEBI	-	carbon source
[Ref.: #69554]	D-mannose ChEBI	-	energy source
[Ref.: #69554]	D-mannose ChEBI	+	carbon source
[Ref.: #69554]	D-mannose ChEBI	+	energy source
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69554]	D-sorbitol ChEBI	-	carbon source
[Ref.: #69554]	D-sorbitol ChEBI	-	energy source
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69554]	dextran ChEBI	+	carbon source
[Ref.: #69554]	dextran ChEBI	+	energy source
[Ref.: #69554]	esculin ChEBI	+	hydrolysis
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69263]	fructose ChEBI	+	growth
[Ref.: #69554]	gelatin ChEBI	+	degradation
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69263]	glucose ChEBI	+	growth
[Ref.: #69554]	glycine ChEBI	+	nitrogen source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69554]	guanine ChEBI	-	degradation
[Ref.: #69554]	hydrogen sulfide ChEBI	-
[Ref.: #69554]	hypoxanthine ChEBI	+	degradation
[Ref.: #69263]	inositol ChEBI	+/-	growth
[Ref.: #69554]	inulin ChEBI	-	carbon source
[Ref.: #69554]	inulin ChEBI	-	energy source
[Ref.: #69554]	L-alanine ChEBI	+	nitrogen source
[Ref.: #69554]	L-arabinose ChEBI	-	carbon source
[Ref.: #69554]	L-arabinose ChEBI	-	energy source
[Ref.: #69554]	L-arginine ChEBI	+	nitrogen source
[Ref.: #69554]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #69554]	L-cysteine ChEBI	+	nitrogen source
[Ref.: #69554]	L-glutamine ChEBI	-	carbon source
[Ref.: #69554]	L-glutamine ChEBI	-	energy source
[Ref.: #69554]	L-histidine ChEBI	-	nitrogen source
[Ref.: #69554]	L-methionine ChEBI	+	nitrogen source
[Ref.: #69554]	L-proline ChEBI	+	nitrogen source
[Ref.: #69554]	L-rhamnose ChEBI	-	carbon source
[Ref.: #69554]	L-rhamnose ChEBI	-	energy source
[Ref.: #69554]	L-serine ChEBI	+	nitrogen source
[Ref.: #69554]	L-sorbose ChEBI	-	carbon source
[Ref.: #69554]	L-sorbose ChEBI	-	energy source
[Ref.: #69554]	L-tyrosine ChEBI	+	nitrogen source
[Ref.: #69554]	L-valine ChEBI	+	nitrogen source
[Ref.: #69554]	lactose ChEBI	-	carbon source
[Ref.: #69554]	lactose ChEBI	-	energy source
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69554]	maltose ChEBI	-	carbon source
[Ref.: #69554]	maltose ChEBI	-	energy source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69263]	mannose ChEBI	+/-	growth
[Ref.: #69554]	melezitose ChEBI	-	carbon source
[Ref.: #69554]	melezitose ChEBI	-	energy source
[Ref.: #69554]	melibiose ChEBI	+	carbon source
[Ref.: #69554]	melibiose ChEBI	+	energy source
[Ref.: #69554]	myo-inositol ChEBI	-	carbon source
[Ref.: #69554]	myo-inositol ChEBI	-	energy source
[Ref.: #69554]	N-glutaryl-L-phenylalanine 2-naphthylamide ChEBI	+	nitrogen source
[Ref.: #69554]	nitrate ChEBI	+/-	reduction
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #69263]	raffinose ChEBI	+/-	growth
[Ref.: #69554]	raffinose ChEBI	+	carbon source
[Ref.: #69554]	raffinose ChEBI	+	energy source
[Ref.: #69263]	rhamnose ChEBI	+	growth
[Ref.: #69554]	starch ChEBI	+	degradation
[Ref.: #69263]	sucrose ChEBI	+	growth
[Ref.: #69554]	sucrose ChEBI	-	carbon source
[Ref.: #69554]	sucrose ChEBI	-	energy source
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69554]	tween 40 ChEBI	-	degradation
[Ref.: #69554]	tween 80 ChEBI	-	degradation
[Ref.: #69554]	urea ChEBI	-	hydrolysis
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69554]	xanthine ChEBI	-	degradation
[Ref.: #69554]	xylitol ChEBI	-	carbon source
[Ref.: #69554]	xylitol ChEBI	-	energy source
[Ref.: #69263]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #69554]		hydrogen sulfide ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69263]	3757	-	-	-	+	-	-	+	+	-	-	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69263]	5941	not determinedn.d.	+	+/-	+/-	+/-	+	+/-	-	-	+	+	-	+/-	+	-	+	-	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65989]	Sample type/isolated from	desert soil
[Ref.: #65989]	Geographic location (country and/or sea, region)	Karakum Desert, Darvaz (40° 15' 38.13'' N, 58° 26' 20.39'' E)
[Ref.: #65989]	Country	Turkmenistan
[Ref.: #65989]	Country ISO 3 Code	TKM
[Ref.: #65989]	Continent	Asia
[Ref.: #65989]	Geographic location	40.2606°/58.4390°

[Ref.: #67771]	Sample type/isolated from	Desert Soil
[Ref.: #67771]	Country	Turkey
[Ref.: #67771]	Country ISO 3 Code	TUR
[Ref.: #67771]	Continent	Asia

[Ref.: #69554]	Sample type/isolated from	desert soil sample collected from the Karakum Desert, Turkmenistan.
[Ref.: #69554]	Geographic location (country and/or sea, region)	Karakum Desert
[Ref.: #69554]	Country	Turkmenistan
[Ref.: #69554]	Country ISO 3 Code	TKM
[Ref.: #69554]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Desert
#Environmental	#Terrestrial	#Soil
#Condition	#Xerophilic	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65989]	Nonomuraea longispora strain KC201 16S ribosomal RNA gene, partial sequence	MG770626	1479	GenBank ENA	1848320 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #69554]	genome sequence	SMJZ00000000		GenBank ENA	1848320 tax ID
[Ref.: #66792]	Nonomuraea longispora KC201	GCA_004348345	contig	GenBank ENA	1848320 tax ID	*
[Ref.: #66792]	Nonomuraea longispora KC201	2866664766	draft	JGI IMG/M	1848320 tax ID	*

[Ref.: #65989]	GC-content	70.6 mol%	sequence analysis
[Ref.: #69554]	GC-content	70.6 mol%	genome sequence analysis

Availability in culture collections External links

[Ref.: #65989]

Culture collection no.

DSM 102917, CGMCC 4.7339, KCTC 39781

[Ref.: #111669]

SI-ID 399093

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Polyphasic classification of Nonomuraea strains isolated from the Karakum Desert and description of Nonomuraea deserti sp. nov., Nonomuraea diastatica sp. nov., Nonomuraea longispora sp. nov. and Nonomuraea mesophila sp. nov.	Saygin H, Nouioui I, Ay H, Guven K, Cetin D, Klenk HP, Goodfellow M, Sahin N	Int J Syst Evol Microbiol	10.1099/ijsem.0.003808	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65989

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 102917

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69263

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69554

Hayrettin Saygin, Imen Nouioui, Hilal Ay, Kiymet Guven, Demet Cetin, Hans-Peter Klen2, Michael Goodfellow and Nevzat Sahin: Polyphasic classification of Nonomuraea strains isolated from the Karakum Desert and description of Nonomuraea deserti sp. nov., Nonomuraea diastatica sp. nov., Nonomuraea longispora sp. nov. and Nonomuraea mesophila sp. nov.. IJSEM 70: 2020 ( DOI 10.1099/ijsem.0.003808 )

#111669

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399093.1 )

* These data were automatically processed and therefore are not curated

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