Name: Dialister hominis 5BBH33
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158927

Type strain:

Species: Dialister hominis

Strain Designation: 5BBH33

Culture col. no.: DSM 109768, JCM 33369

Strain history: M. Sakamoto; RIKEN BRC-JCM, Japan; 5BBH33.

NCBI tax ID(s): 2582419 (species)

SI-ID 398657

DSM 109768

Section

Dialister hominis 5BBH33 is an anaerobe, mesophilic bacterium that forms circular colonies and was isolated from human feces.

colony-forming
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Negativicutes*
	Order	*Veillonellales*
	Family	*Veillonellaceae*
	Genus	*Dialister*
	Species	**Dialister hominis**
	Full Scientific Name (LPSN)	Dialister hominis Sakamoto et al. 2020

Information on morphological and physiological properties Morphology

[Ref.: #69549]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.974

[Ref.: #69549]	Cell length	1.7 µm

[Ref.: #69549]	Cell width	0.8 µm

[Ref.: #69549]	Cell shape	rod-shaped
[Ref.: #69549]	Cell shape	coccus-shaped

[Ref.: #69549]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %93.39

[Ref.: #69549]	Colony size	0.5-1.0 mm
[Ref.: #69549]	Colony color	translucent
[Ref.: #69549]	Colony shape	circular
[Ref.: #69549]	Incubation period	3 days
[Ref.: #69549]	Cultivation medium used	GAM agar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

GAM agar

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Culture medium growth

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #65749]	growth	37 °C
[Ref.: #67770]	growth	37 °C
[Ref.: #69549]	growth	30-37 °C
[Ref.: #69549]	optimum	37 °C

[Ref.: #65749]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #69549]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69549]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %100

[Ref.: #69549]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.938

[Ref.: #69549]	Observation	In the Rapid ID 32A test, all reactions are negative. Small amounts of propionate are detected as the end-product in PY broth. Large amounts of propionate and small amounts of acetate are also detected in PYG broth supplemented with 1 % (w/v) succinate.
[Ref.: #69549]	Observation	No growth occurs on medium containing 20 % (w/v) bile.
[Ref.: #69549]	Observation	Asaccharolytic. Growth is enhanced by adding 1 % (w/v) succinate to the medium.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69549]	esculin ChEBI	-	hydrolysis
[Ref.: #69549]	gelatin ChEBI	-	assimilation
[Ref.: #69549]	indole ChEBI	-
[Ref.: #69549]	succinate ChEBI	+	growth

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance
[Ref.: #69549]	amoxicillin ChEBI	yes
[Ref.: #69549]	bacitracin ChEBI	yes
[Ref.: #69549]	chloramphenicol ChEBI	yes
[Ref.: #69549]	ciprofloxacin ChEBI	yes
[Ref.: #69549]	colistin ChEBI	yes	10	µg
[Ref.: #69549]	erythromycin ChEBI	yes
[Ref.: #69549]	gentamicin ChEBI	yes
[Ref.: #69549]	kanamycin ChEBI	yes	30	µg
[Ref.: #69549]	neomycin ChEBI				yes
[Ref.: #69549]	oxytetracycline ChEBI	yes
[Ref.: #69549]	penicillin ChEBI	yes
[Ref.: #69549]	streptomycin ChEBI				yes
[Ref.: #69549]	vancomycin ChEBI				yes
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Group ID of combined antibiotics	Metabolite	Antibiotic resistance
[Ref.: #69549]		26	sulfamethoxazole ChEBI	yes
[Ref.: #69549]		26	trimethoprim ChEBI	yes

	Metabolite production	Metabolite	Production
[Ref.: #69549]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #69549]	acid phosphatase	+	3.1.3.2
[Ref.: #69549]	alanine arylamidase	-	3.4.11.2
[Ref.: #69549]	alkaline phosphatase	-	3.1.3.1
[Ref.: #69549]	alpha-fucosidase	-	3.2.1.51
[Ref.: #69549]	alpha-galactosidase	-	3.2.1.22
[Ref.: #69549]	alpha-glucosidase	-	3.2.1.20
[Ref.: #69549]	alpha-mannosidase	-	3.2.1.24
[Ref.: #69549]	arginine arylamidase	-
[Ref.: #69549]	beta-galactosidase	-	3.2.1.23
[Ref.: #69549]	beta-glucosidase	-	3.2.1.21
[Ref.: #69549]	beta-glucuronidase	-	3.2.1.31
[Ref.: #69549]	catalase	-	1.11.1.6
[Ref.: #69549]	chymotrypsin	-	3.4.4.5
[Ref.: #69549]	cystine arylamidase	-	3.4.11.3
[Ref.: #69549]	esterase	+
[Ref.: #69549]	esterase Lipase (C 8)	+
[Ref.: #69549]	glycin arylamidase	-
[Ref.: #69549]	histidine arylamidase	-
[Ref.: #69549]	leucine arylamidase	-	3.4.11.1
[Ref.: #69549]	lipase	-
[Ref.: #69549]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #69549]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #69549]	phenylalanine arylamidase	-
[Ref.: #69549]	serine arylamidase	-
[Ref.: #69549]	trypsin	-	3.4.21.4
[Ref.: #69549]	tyrosine arylamidase	-
[Ref.: #69549]	urease	-	3.5.1.5
[Ref.: #69549]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65749]	Sample type/isolated from	human feces
[Ref.: #65749]	Geographic location (country and/or sea, region)	Tsukuba
[Ref.: #65749]	Country	Japan
[Ref.: #65749]	Country ISO 3 Code	JPN
[Ref.: #65749]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Feces of a woman in the forties
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #69549]	Sample type/isolated from	human faeces
[Ref.: #69549]	Geographic location (country and/or sea, region)	Tsukuba
[Ref.: #69549]	Country	Japan
[Ref.: #69549]	Country ISO 3 Code	JPN
[Ref.: #69549]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

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Global distribution of 16S sequence LC487980 (>99% sequence identity) for Dialister from Microbeatlas

Aquatic Samples	5991
Soil Samples	1301
Animal Samples	90501
Plant Samples	513
Total Samples	98306

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65749]	Dialister hominis 5BBH33 gene for 16S ribosomal RNA, partial sequence	LC487980	1504	ENA	2582419 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #69549]	genome sequence	AP019697		GenBank	2582419 tax ID
[Ref.: #66792]	Dialister hominis 5BBH33	GCA_007164725	complete	GenBank	2582419 tax ID	*
[Ref.: #66792]	Dialister sp. 5BBH33	2582419.3	complete	PATRIC	2582419 tax ID	*
[Ref.: #66792]	Dialister hominis 5BBH33	2883940520	complete	JGI IMG/M	2582419 tax ID	*

[Ref.: #67770]	GC-content	48.2 mol%	genome sequence analysis
[Ref.: #69549]	GC-content	42.8 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Dialister hominis 5BBH33 NCBI: GCA_007164725.1
[Ref.: #69481]	spore-forming	no	83	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Dialister hominis 5BBH33 NCBI: GCA_007164725.1
[Ref.: #69480]	motile	no	89.078	no
[Ref.: #69480]	flagellated	no	95.714	no
[Ref.: #69480]	gram-positive	no	95.451	no
[Ref.: #69480]	anaerobic	yes	99.52	no
[Ref.: #69480]	aerobic	no	96.719	no
[Ref.: #69480]	halophile	no	77.635	no
[Ref.: #69480]	spore-forming	no	88.525	no
[Ref.: #69480]	thermophile	no	97.927	yes
[Ref.: #69480]	glucose-util	yes	64.202	no
[Ref.: #69480]	glucose-ferment	no	54.269	no

Availability in culture collections External links

[Ref.: #65749]

Culture collection no.

DSM 109768, JCM 33369

[Ref.: #111446]

SI-ID 398657

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Dialister hominis sp. nov., isolated from human faeces.	Sakamoto M, Ikeyama N, Toyoda A, Murakami T, Mori H, Iino T, Ohkuma M	Int J Syst Evol Microbiol	10.1099/ijsem.0.003797	2020	*
Pathogenicity	Growth of succinate consumer Dialister hominis is supported by Bacteroides thetaiotaomicron.	Sakamoto M, Ikeyama N, Iino T, Ohkuma M	Anaerobe	10.1016/j.anaerobe.2022.102642	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65749

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 109768

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#69549

Mitsuo Sakamoto, Nao Ikeyama, Atsushi Toyoda, Takumi Murakami, Hiroshi Mori, Takao Iino and Moriya Ohkuma: Dialister hominis sp. nov., isolated from human faeces. IJSEM 70: 2020 ( DOI 10.1099/ijsem.0.003797 )

#111446

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398657.1 )

* These data were automatically processed and therefore are not curated

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