Name: Peribacillus saganii V47-23a
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158858

Type strain:

Species: Peribacillus saganii

Strain Designation: V47-23a

Culture col. no.: DSM 105190, ATCC BAA-2861

Strain history: <- P. A. Vaishampayan, Jet Propulsion Laboratory, NASA; V47-23a

NCBI tax ID(s): 2303992 (species)

SI-ID 403221

DSM 105190

Section

Peribacillus saganii V47-23a is an aerobe, spore-forming, mesophilic bacterium that was isolated from Viking Spacecraft.

Gram-positive
motile
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Peribacillus*
	Species	**Peribacillus saganii**
	Full Scientific Name (LPSN)	Peribacillus saganii (Seuylemezian et al. 2020) Gupta et al. 2020

Synonym

Bacillus saganii

Information on morphological and physiological properties Morphology

[Ref.: #69492]	Gram stain	positive

[Ref.: #69492]	Cell length	3.7-4.2 µm

[Ref.: #69492]	Cell width	0.38-0.52 µm

[Ref.: #69492]	Cell shape	rod-shaped

[Ref.: #69492]	Motility	yes

[Ref.: #69492]	Colony size	2 mm
[Ref.: #69492]	Colony color	white
[Ref.: #69492]	Incubation period	2 days
[Ref.: #69492]	Cultivation medium used	Trypticase Soy Agar (TSA)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65680]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #65680]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #65680]

Culture medium composition

expand / minimize

[Ref.: #69492]

Culture medium

Trypticase Soy Agar (TSA)

[Ref.: #69492]

Culture medium growth

		Temperature
[Ref.: #65680]	growth	30 °C
[Ref.: #69492]	growth	20.0-45.0 °C
[Ref.: #69492]	optimum	25 °C

[Ref.: #65680]	Temperature range	mesophilic
[Ref.: #69492]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #69492]			optimum	9
[Ref.: #69492]			growth	5.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69492]

Oxygen tolerance

aerobe

[Ref.: #69492]	Type of spore	endospore
[Ref.: #69492]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69492]		NaCl		growth	0.0-5.0	%(w/v)
[Ref.: #69492]		NaCl		optimum	0	%(w/v)

[Ref.: #69492]	Observation	The polar lipid profile comprises phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol.
[Ref.: #69492]	Observation	The cell-wall peptidoglycan contains meso-diaminopimelic acid.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69492]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #69492]	4-nitrophenyl beta-D-galactopyranoside hydrolysate	-	builds acid from
[Ref.: #69492]	4-nitrophenyl beta-D-galactopyranoside hydrolysate	-	hydrolysis
[Ref.: #69492]	5-dehydro-D-gluconate ChEBI	+	builds acid from
[Ref.: #69492]	adipate ChEBI	-	assimilation
[Ref.: #69492]	amygdalin ChEBI	-	builds acid from
[Ref.: #69492]	arbutin ChEBI	-	builds acid from
[Ref.: #69492]	beta-gentiobiose ChEBI	-	builds acid from
[Ref.: #69492]	cellobiose ChEBI	-	builds acid from
[Ref.: #69492]	D-arabinose ChEBI	-	builds acid from
[Ref.: #69492]	D-arabitol ChEBI	-	builds acid from
[Ref.: #69492]	D-fructose ChEBI	-	builds acid from
[Ref.: #69492]	D-fucose ChEBI	-	builds acid from
[Ref.: #69492]	D-glucose ChEBI	-	assimilation
[Ref.: #69492]	D-glucose ChEBI	+	builds acid from
[Ref.: #69492]	D-lyxose ChEBI	-	builds acid from
[Ref.: #69492]	D-mannitol ChEBI	-	assimilation
[Ref.: #69492]	D-mannose ChEBI	-	assimilation
[Ref.: #69492]	D-mannose ChEBI	-	builds acid from
[Ref.: #69492]	D-tagatose ChEBI	-	builds acid from
[Ref.: #69492]	D-xylose ChEBI	-	builds acid from
[Ref.: #69492]	decanoate ChEBI	-	assimilation
[Ref.: #69492]	erythritol ChEBI	-	builds acid from
[Ref.: #69492]	esculin ChEBI	-	builds acid from
[Ref.: #69492]	esculin ChEBI	-	hydrolysis
[Ref.: #69492]	ferric citrate ChEBI	-	hydrolysis
[Ref.: #69492]	galactitol ChEBI	-	builds acid from
[Ref.: #69492]	galactose ChEBI	-	builds acid from
[Ref.: #69492]	gelatin ChEBI	-	hydrolysis
[Ref.: #69492]	gluconate ChEBI	-	builds acid from
[Ref.: #69492]	glycerol ChEBI	-	builds acid from
[Ref.: #69492]	glycogen ChEBI	+	builds acid from
[Ref.: #69492]	inulin ChEBI	-	builds acid from
[Ref.: #69492]	L-arabinose ChEBI	-	assimilation
[Ref.: #69492]	L-arabinose ChEBI	-	builds acid from
[Ref.: #69492]	L-arginine ChEBI	-	hydrolysis
[Ref.: #69492]	L-fucose ChEBI	-	builds acid from
[Ref.: #69492]	L-sorbose ChEBI	-	builds acid from
[Ref.: #69492]	L-xylose ChEBI	-	builds acid from
[Ref.: #69492]	lactose ChEBI	-	builds acid from
[Ref.: #69492]	malate ChEBI	-	assimilation
[Ref.: #69492]	maltose ChEBI	-	assimilation
[Ref.: #69492]	maltose ChEBI	+	builds acid from
[Ref.: #69492]	mannitol ChEBI	+	builds acid from
[Ref.: #69492]	melezitose ChEBI	-	builds acid from
[Ref.: #69492]	melibiose ChEBI	-	builds acid from
[Ref.: #69492]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #69492]	methyl D-glucoside ChEBI	-	builds acid from
[Ref.: #69492]	myo-inositol ChEBI	-	builds acid from
[Ref.: #69492]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #69492]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #69492]	nitrate ChEBI	-	reduction
[Ref.: #69492]	phenylacetate ChEBI	-	assimilation
[Ref.: #69492]	potassium gluconate ChEBI	-	assimilation
[Ref.: #69492]	raffinose ChEBI	-	builds acid from
[Ref.: #69492]	rhamnose ChEBI	-	builds acid from
[Ref.: #69492]	ribitol ChEBI	-	builds acid from
[Ref.: #69492]	ribose ChEBI	-	builds acid from
[Ref.: #69492]	salicin ChEBI	-	builds acid from
[Ref.: #69492]	sodium citrate ChEBI	-	assimilation
[Ref.: #69492]	sorbitol ChEBI	-	builds acid from
[Ref.: #69492]	starch ChEBI	-	builds acid from
[Ref.: #69492]	sucrose ChEBI	+	builds acid from
[Ref.: #69492]	trehalose ChEBI	+	builds acid from
[Ref.: #69492]	turanose ChEBI	-	builds acid from
[Ref.: #69492]	urea ChEBI	-	hydrolysis
[Ref.: #69492]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #69492]		indole ChEBI	yes

	Enzymes	Enzyme	Enzyme activity
[Ref.: #69492]		gelatinase	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65680]	Sample type/isolated from	Viking Spacecraft
[Ref.: #65680]	Geographic location (country and/or sea, region)	California
[Ref.: #65680]	Country	USA
[Ref.: #65680]	Country ISO 3 Code	USA
[Ref.: #65680]	Continent	North America

[Ref.: #69492]	Sample type/isolated from	Teflon ribbon aerosol sampling within the vehicle assembly building located at Kennedy Space Center
[Ref.: #69492]	Geographic location (country and/or sea, region)	Cape Canaveral, Florida
[Ref.: #69492]	Country	USA
[Ref.: #69492]	Country ISO 3 Code	USA
[Ref.: #69492]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered

#Built environment

#Clean room

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KT720344 (>99% sequence identity) for Bacillus saganii subclade from Microbeatlas

Aquatic Samples	32
Soil Samples	87
Animal Samples	14
Plant Samples	8
Total Samples	141

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65680]	Bacillus sp. V47.23a 16S ribosomal RNA gene, partial sequence	KT720344	1421	ENA	1758622 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Peribacillus saganii V47-23a	GCA_003429095	contig	GenBank	2303992 tax ID	*

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GC-content

40.6 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Peribacillus saganii V47-23a NCBI: GCA_003429095.1
[Ref.: #69480]	motile	yes	91.84	no
[Ref.: #69480]	flagellated	yes	80.629	no
[Ref.: #69480]	gram-positive	yes	85.907	no
[Ref.: #69480]	anaerobic	no	97.273	no
[Ref.: #69480]	aerobic	yes	88.67	no
[Ref.: #69480]	halophile	no	81.344	no
[Ref.: #69480]	spore-forming	yes	94.915	no
[Ref.: #69480]	glucose-util	yes	87.704	no
[Ref.: #69480]	thermophile	no	97.548	yes
[Ref.: #69480]	glucose-ferment	no	92.528	no

Availability in culture collections External links

[Ref.: #65680]

Culture collection no.

DSM 105190, ATCC BAA-2861

[Ref.: #111377]

SI-ID 403221

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequences of Bacillus glennii V44-8, Bacillus saganii V47-23a, Bacillus sp. Strain V59.32b, Bacillus sp. Strain MER_TA_151, and Paenibacillus sp. Strain MER_111, Isolated from Cleanrooms Where the Viking and Mars Exploration Rover Spacecraft Were Assembled.	Becket E, Johnson KO, Burke CJ, Clark JJ, Cohen MJS, Coil DA, Eggleston CA, Farmer TL, Farr TR, Hernandez SM, Jaureguy JP, Jospin G, Khan A, Lee MD, McKee LN, O'Brien EM, Read BA, Saisho R, Seuylemezian A, Serrato-Arroyo SS, Steinecke D, Vaishampayan P	Microbiol Resour Announc	10.1128/MRA.00354-20	2020	*
Phylogeny	Bacillus glennii sp. nov. and Bacillus saganii sp. nov., isolated from the vehicle assembly building at Kennedy Space Center where the Viking spacecraft were assembled.	Seuylemezian A, Ott L, Wolf S, Fragante J, Yip O, Pukall R, Schumann P, Vaishampayan P	Int J Syst Evol Microbiol	10.1099/ijsem.0.003714	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65680

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105190

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69492

Wen-Nan Yu, Zhao-Zhong Du, Ya-Qi Chang, Da-Shuai Mu, Zong-Jun Du: Bacillus glennii sp. nov. and Bacillus saganii sp. nov., isolated from the vehicle assembly building at Kennedy Space Center where the Viking spacecraft were assembled. IJSEM 70: 2020 ( DOI 10.1099/ijsem.0.003714 )

#111377

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403221.1 )

* These data were automatically processed and therefore are not curated

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